{"id":"https://openalex.org/W1968762322","doi":"https://doi.org/10.1042/ba19990107","title":"Development of allergen\u2010coated paper discs for allergy diagnosis by ELISA","display_name":"Development of allergen\u2010coated paper discs for allergy diagnosis by ELISA","publication_year":2000,"publication_date":"2000-08-01","ids":{"openalex":"https://openalex.org/W1968762322","doi":"https://doi.org/10.1042/ba19990107","mag":"1968762322","pmid":"https://pubmed.ncbi.nlm.nih.gov/10918033"},"language":"en","primary_location":{"id":"doi:10.1042/ba19990107","doi":"https://doi.org/10.1042/ba19990107","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba19990107","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071840021","display_name":"Bhanu Singh","orcid":"https://orcid.org/0000-0003-3309-8772","observed_orcids":["https://orcid.org/0000-0003-3309-8772"]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]},{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Bhanu Pratap Singh","raw_affiliation_strings":["Centre for Biochemical Technology, Delhi University Campus, Delhi-1 10007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Biochemical Technology, Delhi University Campus, Delhi-1 10007, India","institution_ids":["https://openalex.org/I110166357","https://openalex.org/I177828482"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103687312","display_name":"Susheela Sridhara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]},{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Susheela Sridhara","raw_affiliation_strings":["Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India","institution_ids":["https://openalex.org/I110166357","https://openalex.org/I177828482"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111529265","display_name":"M Malhotra","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]},{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Mittoo Malhotra","raw_affiliation_strings":["Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India","institution_ids":["https://openalex.org/I110166357","https://openalex.org/I177828482"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062322302","display_name":"N. B. Tulsani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]},{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Narayan B. Tulsani","raw_affiliation_strings":["Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India","institution_ids":["https://openalex.org/I110166357","https://openalex.org/I177828482"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111667890","display_name":"Sharad V. Gangal","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]},{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sharad V. Gangal","raw_affiliation_strings":["Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Biochemical Technology, Delhi University Campus, Delhi-110007, India","institution_ids":["https://openalex.org/I110166357","https://openalex.org/I177828482"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5071840021"],"corresponding_institution_ids":["https://openalex.org/I110166357","https://openalex.org/I177828482"],"apc_list":{"value":3470,"currency":"USD","value_usd":3470},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.11937673,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"32","issue":"1","first_page":"15","last_page":"19"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10877","display_name":"Allergic Rhinitis and Sensitization","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10877","display_name":"Allergic Rhinitis and Sensitization","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10634","display_name":"Food Allergy and Anaphylaxis Research","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10051","display_name":"Asthma and respiratory diseases","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/allergen-sensitization","display_name":"allergen sensitization","score":0.5649999976158142},{"id":"https://openalex.org/keywords/allergic-rhinitis","display_name":"allergic rhinitis","score":0.765999972820282},{"id":"https://openalex.org/keywords/fungal-allergens","display_name":"fungal allergens","score":0.8370000123977661},{"id":"https://openalex.org/keywords/polyvinyl-alcohol","display_name":"polyvinyl alcohol","score":0.8709999918937683},{"id":"https://openalex.org/keywords/glutaraldehyde","display_name":"glutaraldehyde","score":0.9330000281333923},{"id":"https://openalex.org/keywords/prosopis-juliflora","display_name":"Prosopis juliflora","score":0.8569999933242798},{"id":"https://openalex.org/keywords/cutoff-values","display_name":"cut-off values","score":0.8309999704360962},{"id":"https://openalex.org/keywords/assay-reproducibility","display_name":"assay reproducibility","score":0.5490000247955322}],"concepts":[{"id":"https://openalex.org/C2780510475","wikidata":"https://www.wikidata.org/wiki/Q186752","display_name":"Allergen","level":3,"score":0.8652782440185547},{"id":"https://openalex.org/C207480886","wikidata":"https://www.wikidata.org/wiki/Q42982","display_name":"Allergy","level":2,"score":0.6507230997085571},{"id":"https://openalex.org/C2777529624","wikidata":"https://www.wikidata.org/wiki/Q416475","display_name":"Glutaraldehyde","level":2,"score":0.4898190200328827},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4377838373184204},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38879117369651794},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.37617766857147217},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3191949725151062}],"mesh":[{"descriptor_ui":"D000485","descriptor_name":"Allergens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006967","descriptor_name":"Hypersensitivity","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D010209","descriptor_name":"Paper","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011933","descriptor_name":"Reagent Kits, Diagnostic","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":true},{"descriptor_ui":"D012882","descriptor_name":"Skin Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1042/ba19990107","doi":"https://doi.org/10.1042/ba19990107","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba19990107","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:10918033","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10918033","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Clinical Trial"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1568578986","https://openalex.org/W1963998251","https://openalex.org/W1980565188","https://openalex.org/W1981059550","https://openalex.org/W1985506884","https://openalex.org/W1988917156","https://openalex.org/W2004607227","https://openalex.org/W2020304270","https://openalex.org/W2025141800","https://openalex.org/W2044799733","https://openalex.org/W2056500263","https://openalex.org/W2071138083","https://openalex.org/W2072885493","https://openalex.org/W2404350313"],"related_works":["https://openalex.org/W2037620109","https://openalex.org/W2023768042","https://openalex.org/W4230799626","https://openalex.org/W2009022904","https://openalex.org/W2402461455","https://openalex.org/W2094388245","https://openalex.org/W2722164778","https://openalex.org/W2416783229","https://openalex.org/W3186295892","https://openalex.org/W4416827457"],"abstract_inverted_index":{"In":[0],"vitro":[1],"testing":[2],"is":[3,92,131],"useful":[4],"for":[5,47,80,98,107],"detecting":[6],"pollen":[7],"and":[8,38,62,91,118,134,152],"fungal-allergen":[9],"sensitivities":[10],"in":[11,20],"nasobronchial-allergy":[12],"patients.":[13],"However,":[14],"allergen-coated":[15,71,103],"discs":[16,46,53,72,120,156],"or":[17],"solid-phases":[18],"supplied":[19],"kits":[21],"do":[22],"not":[23],"contain":[24],"all":[25],"the":[26,70,95,99],"relevant":[27],"extracts.":[28],"The":[29,76,102],"present":[30],"study":[31],"was":[32,82],"aimed":[33],"to":[34,68,142],"develop":[35],"a":[36],"safe":[37],"credible":[39],"procedure":[40],"of":[41,137,149],"allergen":[42],"coating":[43],"on":[44,94],"paper":[45],"allergy":[48],"diagnosis":[49],"by":[50,85],"ELISA.":[51],"Paper":[52],"were":[54,65],"coated":[55],"with":[56,115,145,154],"indigenous":[57],"allergens":[58],"using":[59,73],"poly(vinyl":[60],"alcohol)":[61],"glutaraldehyde.":[63],"ELISAs":[64],"carried":[66],"out":[67,84],"standardize":[69],"skin-test-positive-patients'":[74],"sera.":[75],"cut-off":[77],"value":[78,125],"(A)":[79],"ELISA":[81,153],"worked":[83],"analysing":[86],"normal":[87],"human":[88],"sera":[89],"(NHS)":[90],"based":[93],"mean+/-2":[96],"S.D.":[97],"NHS":[100],"value.":[101],"discs,":[104,111],"i.e.":[105],"Centre":[106],"Biochemical":[108],"Technology":[109],"(CBT)":[110],"demonstrated":[112],"excellent":[113],"comparison":[114],"plate-binding":[116],"method":[117],"Pharmacia":[119],"[Prosopis":[121],"juliflora":[122],"(mesquite),":[123],"critical":[124],"0.879":[126],"at":[127],"5%":[128],"probability+/-0.599,":[129],"n=11,":[130],"significant].":[132],"Inter-":[133],"Intra-assay":[135],"coefficients":[136],"variations":[138],"ranged":[139],"from":[140],"2.5-14.05%":[141],"4.":[143],"8-11.5%":[144],"different":[146],"allergens.":[147],"Results":[148],"skin":[150],"tests":[151],"CBT":[155],"showed":[157],"60-70%":[158],"correlation.":[159]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}