{"id":"https://openalex.org/W1968758290","doi":"https://doi.org/10.1021/ac00071a007","title":"Use of synthetic peptides as tracer antigens in fluorescence polarization immunoassays of high molecular weight analytes","display_name":"Use of synthetic peptides as tracer antigens in fluorescence polarization immunoassays of high molecular weight analytes","publication_year":1993,"publication_date":"1993-12-01","ids":{"openalex":"https://openalex.org/W1968758290","doi":"https://doi.org/10.1021/ac00071a007","mag":"1968758290","pmid":"https://pubmed.ncbi.nlm.nih.gov/8297026"},"language":"en","primary_location":{"id":"doi:10.1021/ac00071a007","doi":"https://doi.org/10.1021/ac00071a007","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac00071a007","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113255199","display_name":"Ai-Ping. Wei","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ai Ping. Wei","raw_affiliation_strings":["Department of Pharmaceutics, University of Utah, Salt Lake City 84108"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics, University of Utah, Salt Lake City 84108","institution_ids":["https://openalex.org/I223532165"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110906177","display_name":"James N. Herron","orcid":"https://orcid.org/0000-0002-8298-0537","observed_orcids":["https://orcid.org/0000-0002-8298-0537","https://orcid.org/0009-0002-0312-7798"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"James N. Herron","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.9009,"has_fulltext":false,"cited_by_count":37,"citation_normalized_percentile":{"value":0.70867575,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"65","issue":"23","first_page":"3372","last_page":"3377"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.4341000020503998,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.4341000020503998,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.14820000529289246,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.07289999723434448,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescence-polarization-immunoassay","display_name":"fluorescence polarization immunoassay","score":0.9750000238418579},{"id":"https://openalex.org/keywords/human-chorionic-gonadotropin","display_name":"human chorionic gonadotropin","score":0.8610000014305115},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.7860000133514404},{"id":"https://openalex.org/keywords/competitive-immunoassay","display_name":"competitive immunoassay","score":0.8949999809265137},{"id":"https://openalex.org/keywords/antigen-binding-affinity","display_name":"antigen binding affinity","score":0.6019999980926514},{"id":"https://openalex.org/keywords/excited-state-lifetime","display_name":"excited state lifetime","score":0.7450000047683716},{"id":"https://openalex.org/keywords/hydrodynamic-volume","display_name":"hydrodynamic volume","score":0.7599999904632568},{"id":"https://openalex.org/keywords/antibody-screening","display_name":"antibody screening","score":0.4970000088214874}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.927058219909668},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.6434025764465332},{"id":"https://openalex.org/C2780420688","wikidata":"https://www.wikidata.org/wiki/Q759183","display_name":"Immunoassay","level":3,"score":0.6366421580314636},{"id":"https://openalex.org/C111357860","wikidata":"https://www.wikidata.org/wiki/Q903807","display_name":"Fluorescence anisotropy","level":3,"score":0.6240501403808594},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6129171252250671},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.6093409657478333},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.6023448705673218},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.5764865875244141},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5355534553527832},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.4712909758090973},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4692882001399994},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.37375372648239136},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20516258478164673},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.09429502487182617},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.08096551895141602},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000941","descriptor_name":"Antigens","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006063","descriptor_name":"Chorionic Gonadotropin","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006063","descriptor_name":"Chorionic Gonadotropin","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D016231","descriptor_name":"Fluorescence Polarization Immunoassay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/ac00071a007","doi":"https://doi.org/10.1021/ac00071a007","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac00071a007","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:8297026","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8297026","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3353418511","display_name":"PHYSICAL PROPERTIES OF PROTEINS AND POLYNUCLEOTIDES","funder_award_id":"1t32gm008393-01a1","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G7535144004","display_name":null,"funder_award_id":"GM 08393","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8868013234","display_name":null,"funder_award_id":"AI22898","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320319990","display_name":"National Institute for Health and Care Research","ror":"https://ror.org/0187kwz08"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1869905660","https://openalex.org/W1888014428","https://openalex.org/W1966797815","https://openalex.org/W1990806939","https://openalex.org/W1991382584","https://openalex.org/W2006420660","https://openalex.org/W2006446936","https://openalex.org/W2033989186","https://openalex.org/W2041719513","https://openalex.org/W2055708140","https://openalex.org/W2059736963","https://openalex.org/W2065236789","https://openalex.org/W2118699011","https://openalex.org/W2417625787"],"related_works":["https://openalex.org/W120047938","https://openalex.org/W2075926479","https://openalex.org/W2035865130","https://openalex.org/W1991382584","https://openalex.org/W429107595","https://openalex.org/W3199724878","https://openalex.org/W1968494254","https://openalex.org/W2041989691","https://openalex.org/W2075439333","https://openalex.org/W2102467514"],"abstract_inverted_index":{"This":[0,177,217],"paper":[1],"describes":[2],"a":[3,31,101,143,146,151,158],"homogeneous":[4],"immunoassay":[5,105],"based":[6],"on":[7],"fluorescence":[8,103,133],"polarization":[9,104,200],"that":[10],"enables":[11],"subnanomolar":[12],"detection":[13],"of":[14,33,40,67,76,123,145,160,201,223,231,236],"high":[15,86,232],"molecular":[16,233],"weight":[17,234],"analytes.":[18],"A":[19],"monoclonal":[20],"antibody":[21],"(Mab)":[22],"to":[23,82,114,181,209,221,226],"human":[24],"chorionic":[25],"gonadotrophin":[26],"(hCG)":[27],"was":[28,71,89,135,155],"screened":[29],"against":[30],"panel":[32],"221":[34],"synthetic":[35],"peptides":[36],"using":[37],"the":[38,74,77,83,97,115,175,182,189,192,202],"method":[39,218],"Geysen":[41],"et":[42,47,58],"al.":[43,48,59],"(Geysen,":[44],"H.":[45,56],"M.;":[46,57],"J.":[49,60],"Immunol.":[50,62],"Methods":[51],"1987,":[52],"102,":[53],"259-274.":[54],"Geysen,":[55],"Mol.":[61],"1986,":[63],"23,":[64],"709-715).":[65],"One":[66],"these":[68],"peptides,":[69],"which":[70,194],"located":[72],"near":[73],"C-terminus":[75],"hCG":[78,154],"beta":[79],"chain,":[80],"bound":[81],"Mab":[84,117],"with":[85,91,118],"affinity.":[87],"It":[88],"labeled":[90],"tetramethylrhodamine":[92],"(TMR)":[93],"and":[94,191,212,228],"used":[95],"as":[96,142],"tracer":[98,190,204],"antigen":[99],"in":[100,174,185,196,199],"competitive":[102],"(FPIA)":[106],"for":[107],"hCG.":[108],"The":[109],"peptide-TMR":[110],"conjugate":[111],"binds":[112],"specifically":[113],"anti-hCG":[116],"an":[119],"antigen-binding":[120],"affinity":[121],"(Ka)":[122],"1.5":[124],"x":[125,162],"10(7)":[126],"M-1":[127],"at":[128,157],"6":[129],"degrees":[130],"C.":[131],"Its":[132],"intensity":[134],"enhanced":[136],"by":[137],"approximately":[138],"20%":[139],"upon":[140,205],"binding":[141],"result":[144],"prolonged":[147],"excited-state":[148],"lifetime.":[149],"In":[150],"typical":[152],"embodiment,":[153],"determined":[156],"level":[159],"1":[161],"10(-9)":[163],"M":[164],"(95%":[165],"confidence":[166],"limit)--a":[167],"100-fold":[168],"improvement":[169],"over":[170],"similar":[171],"systems":[172],"reported":[173],"literature.":[176],"is":[178,219],"mainly":[179],"attributed":[180],"large":[183,197],"difference":[184],"hydrodynamic":[186],"volume":[187],"between":[188],"antibody,":[193],"resulted":[195],"changes":[198],"peptide":[203],"binding.":[206],"Issues":[207],"related":[208],"sensitivity,":[210],"specificity,":[211],"reversibility":[213],"were":[214],"also":[215],"investigated.":[216],"believed":[220],"be":[222],"significant":[224],"importance":[225],"rapid":[227],"economical":[229],"measurements":[230],"antigens":[235],"clinical":[237],"interest.":[238]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}