{"id":"https://openalex.org/W1968600644","doi":"https://doi.org/10.1016/j.ymben.2012.06.003","title":"Parallel labeling experiments with [U-13C]glucose validate E. coli metabolic network model for 13C metabolic flux analysis","display_name":"Parallel labeling experiments with [U-13C]glucose validate E. coli metabolic network model for 13C metabolic flux analysis","publication_year":2012,"publication_date":"2012-07-06","ids":{"openalex":"https://openalex.org/W1968600644","doi":"https://doi.org/10.1016/j.ymben.2012.06.003","mag":"1968600644","pmid":"https://pubmed.ncbi.nlm.nih.gov/22771935"},"language":"en","primary_location":{"id":"doi:10.1016/j.ymben.2012.06.003","doi":"https://doi.org/10.1016/j.ymben.2012.06.003","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.ymben.2012.06.003","pdf_url":null,"source":{"id":"https://openalex.org/S173328182","display_name":"Metabolic Engineering","issn_l":"1096-7176","issn":["1096-7176","1096-7184"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067930278","display_name":"Robert W. Leighty","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert W. Leighty","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, Newark, DE 19716, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, Newark, DE 19716, USA","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060360737","display_name":"Maciek R. Antoniewicz","orcid":"https://orcid.org/0000-0002-0087-2875","observed_orcids":["https://orcid.org/0000-0002-0087-2875"]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Maciek R. Antoniewicz","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, Newark, DE 19716, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, Newark, DE 19716, USA","institution_ids":["https://openalex.org/I86501945"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5060360737"],"corresponding_institution_ids":["https://openalex.org/I86501945"],"apc_list":{"value":4780,"currency":"USD","value_usd":4780},"apc_paid":null,"fwci":4.6244,"has_fulltext":false,"cited_by_count":85,"citation_normalized_percentile":{"value":0.96124576,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":100},"biblio":{"volume":"14","issue":"5","first_page":"533","last_page":"541"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/13c-metabolic-flux-analysis","display_name":"13C metabolic flux analysis","score":0.9779999852180481},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.875},{"id":"https://openalex.org/keywords/u-13c-glucose","display_name":"[U-13C]glucose","score":0.8859999775886536},{"id":"https://openalex.org/keywords/kinetic-isotope-effects","display_name":"kinetic isotope effects","score":0.8610000014305115},{"id":"https://openalex.org/keywords/isotopic-dilution","display_name":"isotopic dilution","score":0.6899999976158142},{"id":"https://openalex.org/keywords/co2-flux","display_name":"CO2 flux","score":0.7110000252723694},{"id":"https://openalex.org/keywords/model-completeness","display_name":"model completeness","score":0.656000018119812},{"id":"https://openalex.org/keywords/mini-bioreactors","display_name":"mini-bioreactors","score":0.7289999723434448}],"concepts":[{"id":"https://openalex.org/C160941953","wikidata":"https://www.wikidata.org/wiki/Q6822342","display_name":"Metabolic flux analysis","level":3,"score":0.8544889092445374},{"id":"https://openalex.org/C101810790","wikidata":"https://www.wikidata.org/wiki/Q2263094","display_name":"Metabolic network","level":2,"score":0.6642996072769165},{"id":"https://openalex.org/C68709404","wikidata":"https://www.wikidata.org/wiki/Q1134475","display_name":"Flux (metallurgy)","level":2,"score":0.6113677024841309},{"id":"https://openalex.org/C156398188","wikidata":"https://www.wikidata.org/wiki/Q3278929","display_name":"\u03b413C","level":3,"score":0.542803168296814},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.5080947875976562},{"id":"https://openalex.org/C36674120","wikidata":"https://www.wikidata.org/wiki/Q370228","display_name":"Isotopic labeling","level":2,"score":0.47801315784454346},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46704381704330444},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.46661844849586487},{"id":"https://openalex.org/C161573976","wikidata":"https://www.wikidata.org/wiki/Q420277","display_name":"Glucose transporter","level":3,"score":0.46129950881004333},{"id":"https://openalex.org/C2778863792","wikidata":"https://www.wikidata.org/wiki/Q16909918","display_name":"TRACER","level":2,"score":0.42587313055992126},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4072417616844177},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.40401503443717957},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.4033068120479584},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38554656505584717},{"id":"https://openalex.org/C22117777","wikidata":"https://www.wikidata.org/wiki/Q17148629","display_name":"Stable isotope ratio","level":2,"score":0.3300149440765381},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0902058482170105},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.08358722925186157},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08085659146308899},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.07930389046669006},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002247","descriptor_name":"Carbon Isotopes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002247","descriptor_name":"Carbon Isotopes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D048168","descriptor_name":"Escherichia coli K12","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D048168","descriptor_name":"Escherichia coli K12","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D055442","descriptor_name":"Metabolome","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.ymben.2012.06.003","doi":"https://doi.org/10.1016/j.ymben.2012.06.003","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.ymben.2012.06.003","pdf_url":null,"source":{"id":"https://openalex.org/S173328182","display_name":"Metabolic Engineering","issn_l":"1096-7176","issn":["1096-7176","1096-7184"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering","raw_type":"journal-article"},{"id":"pmid:22771935","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22771935","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic engineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/10","display_name":"Reduced inequalities","score":0.5699999928474426}],"study_designs":[],"awards":[{"id":"https://openalex.org/G618755477","display_name":"CAREER: A novel approach for deciphering cellular metabolic phenotypes using tandem mass spectrometry","funder_award_id":"1054120","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1971645053","https://openalex.org/W1976266924","https://openalex.org/W1989310500","https://openalex.org/W1992170087","https://openalex.org/W2002633990","https://openalex.org/W2007728060","https://openalex.org/W2008421457","https://openalex.org/W2014323212","https://openalex.org/W2023617120","https://openalex.org/W2028449239","https://openalex.org/W2030540851","https://openalex.org/W2032283180","https://openalex.org/W2033471108","https://openalex.org/W2044354987","https://openalex.org/W2049759143","https://openalex.org/W2059902696","https://openalex.org/W2065163714","https://openalex.org/W2065401987","https://openalex.org/W2065785909","https://openalex.org/W2067177470","https://openalex.org/W2072280409","https://openalex.org/W2075368931","https://openalex.org/W2076863223","https://openalex.org/W2083807792","https://openalex.org/W2092493462","https://openalex.org/W2096767559","https://openalex.org/W2098824895","https://openalex.org/W2103041540","https://openalex.org/W2127359939","https://openalex.org/W2131812615","https://openalex.org/W2134972743","https://openalex.org/W2148497594","https://openalex.org/W2150975723","https://openalex.org/W2157149907","https://openalex.org/W2160079005","https://openalex.org/W2316274763"],"related_works":["https://openalex.org/W2564510448","https://openalex.org/W1981299471","https://openalex.org/W1781745633","https://openalex.org/W72378364","https://openalex.org/W2970801850","https://openalex.org/W2095203814","https://openalex.org/W7101002179","https://openalex.org/W2067238003","https://openalex.org/W2411445023","https://openalex.org/W2122079849"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":12},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":15},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}