{"id":"https://openalex.org/W1968508196","doi":"https://doi.org/10.1006/bbrc.1994.2022","title":"Replacement of All \u03b1-Domain Lysines with Glutamates Reduces Metallothionein Detoxification Function","display_name":"Replacement of All \u03b1-Domain Lysines with Glutamates Reduces Metallothionein Detoxification Function","publication_year":1994,"publication_date":"1994-07-01","ids":{"openalex":"https://openalex.org/W1968508196","doi":"https://doi.org/10.1006/bbrc.1994.2022","mag":"1968508196","pmid":"https://pubmed.ncbi.nlm.nih.gov/7914080"},"language":"en","primary_location":{"id":"doi:10.1006/bbrc.1994.2022","doi":"https://doi.org/10.1006/bbrc.1994.2022","is_oa":true,"landing_page_url":"https://doi.org/10.1006/bbrc.1994.2022","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1006/bbrc.1994.2022","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072050903","display_name":"Chris W. Cody","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C.W. Cody","raw_affiliation_strings":["Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205-2103","Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Biochem, Baltimore, MD 21205, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205-2103","institution_ids":["https://openalex.org/I145311948"]},{"raw_affiliation_string":"Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Biochem, Baltimore, MD 21205, USA","institution_ids":["https://openalex.org/I145311948"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113561385","display_name":"P. C. Huang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P.C. Huang","raw_affiliation_strings":["Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Biochem, Baltimore, MD 21205, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Biochem, Baltimore, MD 21205, USA","institution_ids":["https://openalex.org/I145311948"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I145311948"],"apc_list":{"value":3110,"currency":"USD","value_usd":3110},"apc_paid":{"value":3110,"currency":"USD","value_usd":3110},"fwci":1.3459,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.76159847,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"202","issue":"2","first_page":"954","last_page":"959"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10915","display_name":"Trace Elements in Health","score":0.9506999850273132,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10915","display_name":"Trace Elements in Health","score":0.9506999850273132,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10790","display_name":"Heavy Metal Exposure and Toxicity","score":0.04050000011920929,"subfield":{"id":"https://openalex.org/subfields/2307","display_name":"Health, Toxicology and Mutagenesis"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12780","display_name":"Heavy Metal Pollution Remediation","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2312","display_name":"Water Science and Technology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metallothionein","display_name":"metallothionein","score":0.9810000061988831},{"id":"https://openalex.org/keywords/metal-binding-domain","display_name":"metal-binding domain","score":0.6169999837875366},{"id":"https://openalex.org/keywords/lysine-residues","display_name":"lysine residues","score":0.7429999709129333},{"id":"https://openalex.org/keywords/glutamate-substitution","display_name":"glutamate substitution","score":0.8519999980926514},{"id":"https://openalex.org/keywords/cadmium-detoxification","display_name":"cadmium detoxification","score":0.7889999747276306},{"id":"https://openalex.org/keywords/metallothionein-2","display_name":"metallothionein-2","score":0.6980000138282776},{"id":"https://openalex.org/keywords/protein-conformation","display_name":"protein conformation","score":0.7179999947547913},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.777999997138977},{"id":"https://openalex.org/keywords/metal-ion-binding","display_name":"metal ion binding","score":0.3970000147819519}],"concepts":[{"id":"https://openalex.org/C20719415","wikidata":"https://www.wikidata.org/wiki/Q416896","display_name":"Metallothionein","level":3,"score":0.7666519284248352},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.753589391708374},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.6561949849128723},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6362142562866211},{"id":"https://openalex.org/C2779349466","wikidata":"https://www.wikidata.org/wiki/Q181619","display_name":"Glutamine","level":3,"score":0.5425721406936646},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5345358848571777},{"id":"https://openalex.org/C2776016237","wikidata":"https://www.wikidata.org/wiki/Q178430","display_name":"Lysine","level":3,"score":0.5226961374282837},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.5067833065986633},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4446987807750702},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44101133942604065},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4389329254627228},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.07810965180397034},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002104","descriptor_name":"Cadmium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002104","descriptor_name":"Cadmium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004351","descriptor_name":"Drug Resistance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005971","descriptor_name":"Glutamates","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008668","descriptor_name":"Metallothionein","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008668","descriptor_name":"Metallothionein","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008668","descriptor_name":"Metallothionein","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1006/bbrc.1994.2022","doi":"https://doi.org/10.1006/bbrc.1994.2022","is_oa":true,"landing_page_url":"https://doi.org/10.1006/bbrc.1994.2022","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},{"id":"pmid:7914080","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7914080","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and biophysical research communications","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1006/bbrc.1994.2022","doi":"https://doi.org/10.1006/bbrc.1994.2022","is_oa":true,"landing_page_url":"https://doi.org/10.1006/bbrc.1994.2022","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Life on land","score":0.46000000834465027,"id":"https://metadata.un.org/sdg/15"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2274559769","display_name":null,"funder_award_id":"GM R01 32606","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8697679407","display_name":"SITE SPECIFIC MUTATION IN METALLOTHIONEIN","funder_award_id":"5r01gm032606-08","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2080940431","https://openalex.org/W2211159822","https://openalex.org/W2025890979","https://openalex.org/W2115624202","https://openalex.org/W2055228066","https://openalex.org/W2008495072","https://openalex.org/W2090691996","https://openalex.org/W1971287848","https://openalex.org/W4379057719","https://openalex.org/W4312336187"],"abstract_inverted_index":{"Mammalian":[0],"metallothioneins":[1],"(MTs)":[2],"possess":[3],"eight":[4],"highly":[5],"conserved":[6],"lysine":[7,100],"residues,":[8],"including":[9],"three":[10,53],"in":[11,27,42,55,109],"each":[12],"of":[13,78,114],"two":[14],"metal":[15,44],"binding":[16],"domains.":[17],"We":[18],"used":[19],"site-directed":[20],"mutagenesis":[21],"to":[22,67,70,93],"replace":[23],"these":[24],"intradomain":[25,105],"lysines":[26,54,106],"Chinese":[28],"hamster":[29],"ovary":[30],"MT2":[31],"with":[32],"glutamic":[33],"acid":[34],"and/or":[35],"glutamine.":[36],"These":[37],"mutant":[38,49,83],"MTs":[39],"were":[40],"expressed":[41],"a":[43,63,89],"sensitive":[45],"yeast":[46,72],"host.":[47],"One":[48],"which":[50],"had":[51],"all":[52],"the":[56,104,111],"alpha-domain":[57],"replaced":[58],"by":[59],"glutamates":[60],"(K43,51,56E)":[61],"exhibited":[62,85],"reduced":[64],"ability,":[65],"relative":[66,92],"native":[68],"MT,":[69],"protect":[71],"transformants":[73],"against":[74],"otherwise":[75],"toxic":[76],"levels":[77],"cadmium.":[79],"This":[80],"triply":[81],"substituted":[82],"also":[84],"anomalous":[86],"migration":[87],"on":[88],"non-denaturing":[90],"gel":[91],"wild":[94],"type":[95],"MT":[96,99],"and":[97],"other":[98],"mutants,":[101],"suggesting":[102],"that":[103],"are":[107],"important":[108],"maintaining":[110],"conformational":[112],"integrity":[113],"MT.":[115]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}