{"id":"https://openalex.org/W1968213697","doi":"https://doi.org/10.1117/12.347530","title":"Spectral properties and biological applications of ELF enzyme substrates that yield fluorescent precipitates at the enzymatic activity sites","display_name":"Spectral properties and biological applications of ELF enzyme substrates that yield fluorescent precipitates at the enzymatic activity sites","publication_year":1999,"publication_date":"1999-05-03","ids":{"openalex":"https://openalex.org/W1968213697","doi":"https://doi.org/10.1117/12.347530","mag":"1968213697"},"language":"en","primary_location":{"id":"doi:10.1117/12.347530","doi":"https://doi.org/10.1117/12.347530","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.347530","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110619228","display_name":"Zhenjun J. Diwu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhenjun Diwu","raw_affiliation_strings":["Molecular Probes, Inc. (United States)","Molecular Probes, Inc., USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Probes, Inc. (United States)","institution_ids":[]},{"raw_affiliation_string":"Molecular Probes, Inc., USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061374782","display_name":"Dieter H. Klaubert","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dieter H. Klaubert","raw_affiliation_strings":["Molecular Probes, Inc. (United States)","Molecular Probes, Inc., USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Probes, Inc. (United States)","institution_ids":[]},{"raw_affiliation_string":"Molecular Probes, Inc., USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112868059","display_name":"Richard P. Haugland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Richard P. Haugland","raw_affiliation_strings":["Molecular Probes, Inc. (United States)","Molecular Probes, Inc., USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Probes, Inc. (United States)","institution_ids":[]},{"raw_affiliation_string":"Molecular Probes, Inc., USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.06797583,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"3602","issue":null,"first_page":"265","last_page":"274"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10532","display_name":"Molecular Sensors and Ion Detection","score":0.5990999937057495,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10532","display_name":"Molecular Sensors and Ion Detection","score":0.5990999937057495,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.2409999966621399,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12318","display_name":"Quinazolinone synthesis and applications","score":0.03229999914765358,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorogenic-substrates","display_name":"fluorogenic substrates","score":0.7850000262260437},{"id":"https://openalex.org/keywords/enzyme-substrates","display_name":"enzyme substrates","score":0.7390000224113464},{"id":"https://openalex.org/keywords/quinazolinones","display_name":"quinazolinone derivatives","score":0.7720000147819519},{"id":"https://openalex.org/keywords/fluorescent-histochemistry","display_name":"fluorescent histochemistry","score":0.7799999713897705},{"id":"https://openalex.org/keywords/enzyme-activity-imaging","display_name":"enzyme activity imaging","score":0.4699999988079071},{"id":"https://openalex.org/keywords/photostability","display_name":"photostability","score":0.593999981880188},{"id":"https://openalex.org/keywords/spectral-properties","display_name":"spectral properties","score":0.48100000619888306}],"concepts":[{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.814383864402771},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.7764773368835449},{"id":"https://openalex.org/C2778015030","wikidata":"https://www.wikidata.org/wiki/Q410922","display_name":"Fluorescein","level":3,"score":0.6629722714424133},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6372843384742737},{"id":"https://openalex.org/C2781338262","wikidata":"https://www.wikidata.org/wiki/Q111812","display_name":"Coumarin","level":2,"score":0.5880041122436523},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.5503296256065369},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5326679944992065},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.47808265686035156},{"id":"https://openalex.org/C2779020807","wikidata":"https://www.wikidata.org/wiki/Q416196","display_name":"Umbelliferone","level":3,"score":0.47625136375427246},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4272410571575165},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3444458246231079},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.17836058139801025},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.14833274483680725},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09125575423240662},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1117/12.347530","doi":"https://doi.org/10.1117/12.347530","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.347530","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Clean water and sanitation","score":0.7900000214576721,"id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W141236626","https://openalex.org/W1856246924","https://openalex.org/W1990853271","https://openalex.org/W1994588414","https://openalex.org/W2010664095","https://openalex.org/W2019956094","https://openalex.org/W2039334537","https://openalex.org/W2068806073","https://openalex.org/W2155135296","https://openalex.org/W2165055481","https://openalex.org/W2167403120","https://openalex.org/W2168467291","https://openalex.org/W2204617243","https://openalex.org/W2407584298","https://openalex.org/W2949343483"],"related_works":["https://openalex.org/W2115880913","https://openalex.org/W2952418178","https://openalex.org/W1990020765","https://openalex.org/W2395699093","https://openalex.org/W2168478301","https://openalex.org/W2082521727","https://openalex.org/W1918355422","https://openalex.org/W6921780690","https://openalex.org/W358291488","https://openalex.org/W1988063409"],"abstract_inverted_index":{"Although":[0],"coumarin-":[1,51],"and":[2,36,52,58,68,97,103,131,137,142],"fluorescein-based":[3,53],"fluorogenic":[4,54,118],"substrates":[5,55,87,99,120],"are":[6,16,56,69],"widely":[7],"used":[8],"in":[9,22,73],"a":[10,80],"variety":[11,81],"of":[12,25,50,65,82,112,125,144],"enzyme":[13,66,86],"assays,":[14],"they":[15],"still":[17],"ill-adapted":[18],"for":[19,72],"continuous":[20],"assays":[21],"the":[23,40,47,63,110,113,145],"cases":[24],"enzymes":[26,126],"that":[27,88],"have":[28,39,77,89],"optimal":[29],"activity":[30],"below":[31],"pH":[32,44],"7.0":[33],"because":[34],"coumarins":[35],"fluoresceins":[37],"generally":[38],"maximum":[41],"fluorescence":[42],"above":[43],"10.":[45],"Additionally":[46],"enzymatic":[48,107],"products":[49],"soluble":[57],"readily":[59],"diffuse":[60],"away":[61],"from":[62],"site":[64],"activity,":[67],"especially":[70],"troublesome":[71],"vivo":[74],"applications.":[75],"We":[76],"now":[78],"developed":[79],"2-(2'-":[83],"hydroxyphenyl)-4(3H)-quinazolinone":[84],"(HPQ)-based":[85],"overcome":[90],"these":[91],"limitations.":[92],"The":[93],"water-":[94],"soluble,":[95],"colorless,":[96],"nonfluorescent":[98],"generate":[100],"highly":[101],"fluorescent":[102,122],"photostable":[104],"precipitates":[105],"upon":[106],"action":[108],"without":[109],"addition":[111],"secondary":[114],"trapping":[115],"agents.":[116],"Such":[117],"precipitating":[119],"ensure":[121],"histochemical":[123],"imaging":[124],"with":[127],"cytologically":[128],"fine":[129],"resolution":[130],"sharp":[132],"contrast":[133],"over":[134],"cellular":[135],"autofluorescence":[136],"therefore":[138],"inherently":[139],"enhance":[140],"sensitivity":[141],"specificity":[143],"targeted":[146],"analysis.":[147]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}
