{"id":"https://openalex.org/W1968178019","doi":"https://doi.org/10.1074/jbc.272.26.16453","title":"Identification of Transcription Factor Binding Sites Important in the Regulation of the Human Interleukin-5 Gene","display_name":"Identification of Transcription Factor Binding Sites Important in the Regulation of the Human Interleukin-5 Gene","publication_year":1997,"publication_date":"1997-06-01","ids":{"openalex":"https://openalex.org/W1968178019","doi":"https://doi.org/10.1074/jbc.272.26.16453","mag":"1968178019","pmid":"https://pubmed.ncbi.nlm.nih.gov/9195954"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.272.26.16453","doi":"https://doi.org/10.1074/jbc.272.26.16453","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.272.26.16453","pdf_url":"http://www.jbc.org/article/S0021925818701221/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925818701221/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087394240","display_name":"Kimberly S. Stranick","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Kimberly S. Stranick","raw_affiliation_strings":["Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA. kimberly.stranick@spcorp.com","From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA. kimberly.stranick@spcorp.com","institution_ids":[]},{"raw_affiliation_string":"From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032351192","display_name":"Demetris N. Zambas","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Demetris N. Zambas","raw_affiliation_strings":["Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]},{"raw_affiliation_string":"From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053706599","display_name":"Annette Schettino Uss","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Annette Schettino Uss","raw_affiliation_strings":["Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]},{"raw_affiliation_string":"From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046002593","display_name":"Robert W. Egan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Robert W. Egan","raw_affiliation_strings":["Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]},{"raw_affiliation_string":"From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111104506","display_name":"M. Motasim Billah","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M. Motasim Billah","raw_affiliation_strings":["Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]},{"raw_affiliation_string":"From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111559545","display_name":"Shelby P. Umland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shelby P. Umland","raw_affiliation_strings":["Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Allergy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033","institution_ids":[]},{"raw_affiliation_string":"From the Department of Allergy, Schering-Plough Research Institute, Kenilworth, New\u2009Jersey\u200907033","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5087394240"],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.369,"has_fulltext":true,"cited_by_count":53,"citation_normalized_percentile":{"value":0.89111205,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":96},"biblio":{"volume":"272","issue":"26","first_page":"16453","last_page":"16465"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12620","display_name":"Signaling Pathways in Disease","score":0.9648000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12620","display_name":"Signaling Pathways in Disease","score":0.9648000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.03280000016093254,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/il-5","display_name":"IL-5","score":0.9210000038146973},{"id":"https://openalex.org/keywords/transcription-factor-binding-sites","display_name":"transcription factor binding sites","score":0.9879999756813049},{"id":"https://openalex.org/keywords/response-elements","display_name":"response elements","score":0.718999981880188},{"id":"https://openalex.org/keywords/t-cell-activation","display_name":"T cell activation","score":0.7480000257492065},{"id":"https://openalex.org/keywords/negative-regulatory-element","display_name":"negative regulatory element","score":0.753000020980835},{"id":"https://openalex.org/keywords/cyclosporine","display_name":"cyclosporine","score":0.925000011920929}],"concepts":[{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.655709445476532},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.5809741020202637},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5319322347640991},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.480782687664032},{"id":"https://openalex.org/C3662595","wikidata":"https://www.wikidata.org/wiki/Q5205743","display_name":"DNA binding site","level":5,"score":0.4563087522983551},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.42273035645484924},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.4107985198497772},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40551403164863586},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34753668308258057},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3075123429298401},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.2964167296886444},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007376","descriptor_name":"Interleukin-2","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014157","descriptor_name":"Transcription Factors","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D015848","descriptor_name":"Interleukin-5","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016572","descriptor_name":"Cyclosporine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D050778","descriptor_name":"NFATC Transcription Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.272.26.16453","doi":"https://doi.org/10.1074/jbc.272.26.16453","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.272.26.16453","pdf_url":"http://www.jbc.org/article/S0021925818701221/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:9195954","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9195954","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.272.26.16453","doi":"https://doi.org/10.1074/jbc.272.26.16453","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.272.26.16453","pdf_url":"http://www.jbc.org/article/S0021925818701221/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1968178019.pdf","grobid_xml":"https://content.openalex.org/works/W1968178019.grobid-xml"},"referenced_works_count":63,"referenced_works":["https://openalex.org/W210641201","https://openalex.org/W236654392","https://openalex.org/W1504084694","https://openalex.org/W1514209228","https://openalex.org/W1572269513","https://openalex.org/W1573672011","https://openalex.org/W1590787771","https://openalex.org/W1593158365","https://openalex.org/W1651302448","https://openalex.org/W1772298148","https://openalex.org/W1827812119","https://openalex.org/W1836845228","https://openalex.org/W1918266073","https://openalex.org/W1919937099","https://openalex.org/W1946763737","https://openalex.org/W1964494877","https://openalex.org/W1972922050","https://openalex.org/W1973253292","https://openalex.org/W1974464589","https://openalex.org/W1975092015","https://openalex.org/W1988227673","https://openalex.org/W1989080074","https://openalex.org/W1993243592","https://openalex.org/W1999248691","https://openalex.org/W2002485919","https://openalex.org/W2009081607","https://openalex.org/W2012597218","https://openalex.org/W2012612191","https://openalex.org/W2017122238","https://openalex.org/W2017718332","https://openalex.org/W2028727751","https://openalex.org/W2039766781","https://openalex.org/W2045722811","https://openalex.org/W2054639404","https://openalex.org/W2056527553","https://openalex.org/W2056666301","https://openalex.org/W2060543236","https://openalex.org/W2061029168","https://openalex.org/W2062567403","https://openalex.org/W2063096720","https://openalex.org/W2064969462","https://openalex.org/W2076338812","https://openalex.org/W2078198072","https://openalex.org/W2078254067","https://openalex.org/W2081135713","https://openalex.org/W2083692829","https://openalex.org/W2089019735","https://openalex.org/W2092957238","https://openalex.org/W2093339251","https://openalex.org/W2093760702","https://openalex.org/W2120478829","https://openalex.org/W2124473841","https://openalex.org/W2125118355","https://openalex.org/W2128635872","https://openalex.org/W2133438408","https://openalex.org/W2144634347","https://openalex.org/W2147551041","https://openalex.org/W2162010631","https://openalex.org/W2172466148","https://openalex.org/W2227294536","https://openalex.org/W2314225431","https://openalex.org/W2413011293","https://openalex.org/W4236478138"],"related_works":["https://openalex.org/W2045722811","https://openalex.org/W2054215910","https://openalex.org/W1977093117","https://openalex.org/W1520499963","https://openalex.org/W1991338307","https://openalex.org/W2008800346","https://openalex.org/W2093760702","https://openalex.org/W2157972566","https://openalex.org/W2063191111","https://openalex.org/W2126122040"],"abstract_inverted_index":{"This":[0],"study":[1],"identifies":[2],"three":[3],"regions":[4],"of":[5,42,64,115,173,177,183,188],"the":[6,31,104,116,134,146,174,191],"human":[7,32],"interleukin":[8],"(IL)-5":[9],"promoter":[10,93],"involved":[11,148],"in":[12,79,95,99,133,149],"binding":[13,189],"nuclear":[14,28,175],"factors":[15,185],"from":[16,30,200],"activated":[17,178],"T":[18,33,80,101,179],"cells.":[19,81],"DNase":[20],"I":[21],"footprinting":[22],"and":[23,51,55,58,60,68,125,152],"mobility":[24],"shift":[25],"assays":[26,98,127],"with":[27,40,128],"proteins":[29],"cell":[34],"clone,":[35],"SP-B21,":[36],"demonstrated":[37],"protein":[38,161],"interactions":[39],"each":[41],"these":[43,65],"response":[44],"elements":[45],"(REs),":[46],"located":[47],"between":[48],"positions":[49],"-79":[50],"-45":[52],"(RE-I),":[53],"-123":[54],"-92":[56],"(RE-II),":[57],"-170":[59],"-130":[61],"(RE-III).":[62],"Two":[63],"regions,":[66],"RE-II":[67,83,117,135,193],"RE-III,":[69],"have":[70,144,153],"not":[71],"previously":[72,201],"been":[73],"described":[74],"to":[75,87,165,190],"regulate":[76],"IL-5":[77,92,129,192],"expression":[78],"The":[82,113],"site":[84,106,118,136],"was":[85,119],"shown":[86],"be":[88,198],"critical":[89],"for":[90],"inducible":[91,159],"activity":[94,114],"transient":[96],"transfection":[97,126],"D10.G4.1":[100],"cells,":[102],"while":[103],"RE-III":[105],"functions":[107],"as":[108,156,158],"a":[109],"negative":[110],"regulatory":[111],"element.":[112],"specifically":[120],"inhibited":[121],"by":[122,205],"cyclosporin":[123],"A,":[124],"constructs":[130],"containing":[131],"mutations":[132],"showed":[137],"greatly":[138],"reduced":[139],"reporter":[140],"gene":[141],"activity.":[142],"We":[143],"defined":[145],"sequence":[147],"stimulation-dependent":[150],"transcription":[151,184],"identified":[154,202],"constitutive":[155],"well":[157],"DNA-binding":[160],"complexes":[162,204],"that":[163],"bind":[164],"RE-II.":[166],"Antibodies":[167],"against":[168],"at":[169],"least":[170],"two":[171],"members":[172],"factor":[176],"cells":[180],"(NFAT)":[181],"family":[182],"are":[186],"capable":[187],"complexes,":[194],"although":[195],"they":[196],"can":[197],"distinguished":[199],"NFAT-specific":[203],"several":[206],"characteristics.":[207]},"counts_by_year":[{"year":2018,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}