{"id":"https://openalex.org/W1967487532","doi":"https://doi.org/10.1364/oe.16.004237","title":"Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding","display_name":"Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding","publication_year":2008,"publication_date":"2008-03-13","ids":{"openalex":"https://openalex.org/W1967487532","doi":"https://doi.org/10.1364/oe.16.004237","mag":"1967487532","pmid":"https://pubmed.ncbi.nlm.nih.gov/18542519"},"language":"en","primary_location":{"id":"doi:10.1364/oe.16.004237","doi":"https://doi.org/10.1364/oe.16.004237","is_oa":true,"landing_page_url":"https://doi.org/10.1364/oe.16.004237","pdf_url":null,"source":{"id":"https://openalex.org/S178776955","display_name":"Optics Express","issn_l":"1094-4087","issn":["1094-4087"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics Express","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1364/oe.16.004237","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030942630","display_name":"Thomas H. Chia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas H. Chia","raw_affiliation_strings":["Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT 06511, USA","Dept. of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT 06511, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT 06511, USA","institution_ids":["https://openalex.org/I32971472"]},{"raw_affiliation_string":"Dept. of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT 06511, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048225812","display_name":"Anne Williamson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anne Williamson","raw_affiliation_strings":["Department of Neurosurgery, Yale University, 333 Cedar St., New Haven, CT 06511, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, Yale University, 333 Cedar St., New Haven, CT 06511, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070693463","display_name":"Dennis D. Spencer","orcid":"https://orcid.org/0000-0002-1276-0467","observed_orcids":["https://orcid.org/0000-0002-1276-0467"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dennis D. Spencer","raw_affiliation_strings":["Department of Neurosurgery, Yale University, 333 Cedar St., New Haven, CT 06511, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, Yale University, 333 Cedar St., New Haven, CT 06511, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008543506","display_name":"Michael J. Levene","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael J. Levene","raw_affiliation_strings":["Dept. of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT 06511, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, CT 06511, USA","institution_ids":["https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I32971472"],"apc_list":{"value":2270,"currency":"USD","value_usd":2270},"apc_paid":{"value":2270,"currency":"USD","value_usd":2270},"fwci":3.2838,"has_fulltext":false,"cited_by_count":71,"citation_normalized_percentile":{"value":0.91814201,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"16","issue":"6","first_page":"4237","last_page":"4237"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nadh","display_name":"NADH","score":0.9440000057220459},{"id":"https://openalex.org/keywords/intrinsic-fluorescence","display_name":"intrinsic fluorescence","score":0.7879999876022339},{"id":"https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy","display_name":"fluorescence lifetime imaging","score":0.9020000100135803},{"id":"https://openalex.org/keywords/two-photon-microscopy","display_name":"two-photon microscopy","score":0.8709999918937683},{"id":"https://openalex.org/keywords/nadh-binding","display_name":"NADH binding","score":0.847000002861023},{"id":"https://openalex.org/keywords/brain-tissue","display_name":"brain tissue","score":0.9129999876022339},{"id":"https://openalex.org/keywords/epilepsy","display_name":"epilepsy","score":0.5649999976158142},{"id":"https://openalex.org/keywords/cerebral-metabolism","display_name":"brain metabolism","score":0.7680000066757202},{"id":"https://openalex.org/keywords/image-processing-techniques","display_name":"image processing algorithms","score":0.800000011920929},{"id":"https://openalex.org/keywords/human-brain-slices","display_name":"human brain slices","score":0.6809999942779541},{"id":"https://openalex.org/keywords/rat-brain-slices","display_name":"rat brain slices","score":0.968999981880188}],"concepts":[{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.8555222153663635},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.6852060556411743},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5207284092903137},{"id":"https://openalex.org/C46141821","wikidata":"https://www.wikidata.org/wiki/Q209402","display_name":"Nuclear magnetic resonance","level":1,"score":0.353908896446228},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33918243646621704},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.3056272864341736},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2566998600959778},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.14913958311080933}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007090","descriptor_name":"Image Interpretation, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007090","descriptor_name":"Image Interpretation, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007090","descriptor_name":"Image Interpretation, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014018","descriptor_name":"Tissue Distribution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014018","descriptor_name":"Tissue Distribution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014018","descriptor_name":"Tissue Distribution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036641","descriptor_name":"Microscopy, Fluorescence, Multiphoton","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D036641","descriptor_name":"Microscopy, Fluorescence, Multiphoton","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D036641","descriptor_name":"Microscopy, Fluorescence, Multiphoton","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1364/oe.16.004237","doi":"https://doi.org/10.1364/oe.16.004237","is_oa":true,"landing_page_url":"https://doi.org/10.1364/oe.16.004237","pdf_url":null,"source":{"id":"https://openalex.org/S178776955","display_name":"Optics Express","issn_l":"1094-4087","issn":["1094-4087"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics Express","raw_type":"journal-article"},{"id":"pmid:18542519","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18542519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics express","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1364/oe.16.004237","doi":"https://doi.org/10.1364/oe.16.004237","is_oa":true,"landing_page_url":"https://doi.org/10.1364/oe.16.004237","pdf_url":null,"source":{"id":"https://openalex.org/S178776955","display_name":"Optics Express","issn_l":"1094-4087","issn":["1094-4087"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics Express","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G538936467","display_name":null,"funder_award_id":"R01-NS054038","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G5482452404","display_name":null,"funder_award_id":"NS054038","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G7666978570","display_name":"Origin of Extracellular Glutamate in Human Temporal Lobe Epilepsy","funder_award_id":"5r01ns054038-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W1964867265","https://openalex.org/W1979968811","https://openalex.org/W1981101976","https://openalex.org/W1984333244","https://openalex.org/W1984853516","https://openalex.org/W1986622270","https://openalex.org/W1987741147","https://openalex.org/W2001170557","https://openalex.org/W2030286287","https://openalex.org/W2032924239","https://openalex.org/W2051011708","https://openalex.org/W2062149105","https://openalex.org/W2072376785","https://openalex.org/W2073890361","https://openalex.org/W2094633691","https://openalex.org/W2095138980","https://openalex.org/W2111920179","https://openalex.org/W2135572946","https://openalex.org/W2139155172","https://openalex.org/W2143647842","https://openalex.org/W2165807302","https://openalex.org/W2166168429","https://openalex.org/W2168846506","https://openalex.org/W2337154557","https://openalex.org/W2409927356","https://openalex.org/W2412260864"],"related_works":["https://openalex.org/W2382309260","https://openalex.org/W2369913389","https://openalex.org/W1968431014","https://openalex.org/W2353900815","https://openalex.org/W2149482486","https://openalex.org/W2053588607","https://openalex.org/W4246308838","https://openalex.org/W2080243894","https://openalex.org/W943367353","https://openalex.org/W2091121756"],"abstract_inverted_index":{"Two-photon":[0],"fluorescence":[1,41,54],"lifetime":[2,26,42],"imaging":[3],"(FLIM)":[4],"of":[5,58,78,98],"molecules":[6],"can":[7,100],"reveal":[8],"important":[9],"information":[10,102],"on":[11,30,103],"the":[12,82],"local":[13],"microenvironment.":[14],"NADH,":[15],"an":[16,48],"intrinsic":[17],"fluorescent":[18],"molecule":[19],"and":[20,43,60,70,87],"ubiquitous":[21],"metabolic":[22,89,104],"co-enzyme,":[23],"has":[24],"a":[25,35],"that":[27,46,84],"depends":[28],"strongly":[29],"enzymatic":[31],"binding.":[32],"We":[33],"present":[34],"custom":[36],"image-processing":[37],"algorithm":[38],"for":[39],"raw":[40],"amplitude":[44],"data":[45],"produces":[47],"image":[49],"showing":[50],"spatially":[51],"distinct":[52],"NADH":[53,63,79,85],"lifetimes":[55,80],"in":[56,68,75,106],"slices":[57],"rat":[59,72],"human":[61],"brain.":[62],"FLIM":[64],"images":[65],"were":[66],"collected":[67],"control":[69],"epileptic":[71],"tissue.":[73],"Differences":[74],"spatial":[76],"patterns":[77],"support":[81],"hypothesis":[83],"binding,":[86],"thus":[88],"capacity,":[90],"is":[91],"significantly":[92],"different":[93],"between":[94],"groups.":[95],"This":[96],"type":[97],"analysis":[99],"provide":[101],"states":[105],"pathological":[107],"material.":[108]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":5}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}
