{"id":"https://openalex.org/W1967082011","doi":"https://doi.org/10.1073/pnas.80.4.1101","title":"Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.","display_name":"Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.","publication_year":1983,"publication_date":"1983-02-01","ids":{"openalex":"https://openalex.org/W1967082011","doi":"https://doi.org/10.1073/pnas.80.4.1101","mag":"1967082011","pmid":"https://pubmed.ncbi.nlm.nih.gov/6405380","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/393536"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.80.4.1101","doi":"https://doi.org/10.1073/pnas.80.4.1101","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.80.4.1101","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W1967082011"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC393536","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014782581","display_name":"Mark M. Zukowski","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M M Zukowski","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109092920","display_name":"Dairena Gaffney","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D F Gaffney","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021415400","display_name":"Denis Speck","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Speck","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081597001","display_name":"Muriel Kauffmann","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Kauffmann","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040114795","display_name":"Annie Findeli","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Findeli","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030522607","display_name":"Anne Wisecup","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Wisecup","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5054563796","display_name":"Jean\u2010Pierre Lecocq","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J P Lecocq","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.8855,"has_fulltext":false,"cited_by_count":283,"citation_normalized_percentile":{"value":0.95961756,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"80","issue":"4","first_page":"1101","last_page":"1105"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacillus-subtilis","display_name":"Bacillus subtilis","score":0.9580000042915344},{"id":"https://openalex.org/keywords/catechol-2-3-dioxygenase","display_name":"catechol 2,3-dioxygenase","score":0.9959999918937683},{"id":"https://openalex.org/keywords/promoter-recognition","display_name":"promoter identification","score":0.48100000619888306},{"id":"https://openalex.org/keywords/ribosome-binding-site","display_name":"ribosome binding site","score":0.7649999856948853},{"id":"https://openalex.org/keywords/expression-vectors","display_name":"expression vectors","score":0.5450000166893005},{"id":"https://openalex.org/keywords/catechol","display_name":"catechol","score":0.5350000262260437},{"id":"https://openalex.org/keywords/2-hydroxymuconic-semialdehyde","display_name":"2-hydroxymuconic semialdehyde","score":0.9340000152587891}],"concepts":[{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.8748437166213989},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7927031517028809},{"id":"https://openalex.org/C2780543182","wikidata":"https://www.wikidata.org/wiki/Q2738168","display_name":"Pseudomonas putida","level":3,"score":0.7370014190673828},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6459729075431824},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6365342736244202},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5955471992492676},{"id":"https://openalex.org/C114847915","wikidata":"https://www.wikidata.org/wiki/Q16948788","display_name":"Ribosomal binding site","level":5,"score":0.5763582587242126},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5096352100372314},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.4195464551448822},{"id":"https://openalex.org/C58301748","wikidata":"https://www.wikidata.org/wiki/Q1074969","display_name":"Restriction site","level":4,"score":0.4121430814266205},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36604028940200806},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.3492923378944397},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.3057026267051697},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.29430291056632996},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.17589527368545532},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1615653932094574},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.07986345887184143}],"mesh":[{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005809","descriptor_name":"Genes, Regulator","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010105","descriptor_name":"Oxygenases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D049308","descriptor_name":"Dioxygenases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D050557","descriptor_name":"Catechol 2,3-Dioxygenase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1073/pnas.80.4.1101","doi":"https://doi.org/10.1073/pnas.80.4.1101","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.80.4.1101","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:64835","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC393536","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:6405380","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6405380","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:393536","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC393536/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"mag:2494780102","doi":null,"is_oa":false,"landing_page_url":"https://www.jstor.org/stable/13891","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:europepmc.org:64835","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC393536","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W123626623","https://openalex.org/W174142848","https://openalex.org/W308347375","https://openalex.org/W1482636423","https://openalex.org/W1515262184","https://openalex.org/W1546783991","https://openalex.org/W1571497143","https://openalex.org/W1692010594","https://openalex.org/W1765985509","https://openalex.org/W1775749144","https://openalex.org/W1862034987","https://openalex.org/W1927972199","https://openalex.org/W1949490448","https://openalex.org/W1952635464","https://openalex.org/W1966058599","https://openalex.org/W1980279189","https://openalex.org/W1980481823","https://openalex.org/W1981777358","https://openalex.org/W1984640298","https://openalex.org/W1986002586","https://openalex.org/W1989676333","https://openalex.org/W2013189569","https://openalex.org/W2018949113","https://openalex.org/W2019410656","https://openalex.org/W2021753888","https://openalex.org/W2026626836","https://openalex.org/W2037457710","https://openalex.org/W2047489267","https://openalex.org/W2054468433","https://openalex.org/W2059297512","https://openalex.org/W2067808653","https://openalex.org/W2111301002","https://openalex.org/W2132481027","https://openalex.org/W2141809638","https://openalex.org/W2144203019","https://openalex.org/W2156402236","https://openalex.org/W2172003650","https://openalex.org/W7097930909"],"related_works":["https://openalex.org/W1692010594","https://openalex.org/W2418059293","https://openalex.org/W2130363308","https://openalex.org/W2155538554","https://openalex.org/W1965707517","https://openalex.org/W2957506814","https://openalex.org/W2141809638","https://openalex.org/W2132513354","https://openalex.org/W1496715030"],"abstract_inverted_index":{"A":[0],"method":[1,121],"to":[2,44,50,105,149,215],"isolate":[3],"fragments":[4],"of":[5,22,71,74,90,116,122,125,159,194,225],"DNA":[6,72,92],"that":[7,127,144,174,188],"promote":[8,106],"gene":[9,38,41,76,108,218],"expression":[10,77,109,219],"in":[11,58,65,110,178,197],"Bacillus":[12,96,98],"subtilis":[13,67,180],"is":[14,18,55,62,78,119,145,161],"described.":[15],"The":[16,40,113,155],"system":[17,118],"based":[19],"on":[20,84],"production":[21],"catechol":[23],"2,3-dioxygenase":[24],"[CatO2ase;":[25],"catechol:oxygen":[26],"2,3-oxidoreductase":[27],"(decyclizing),":[28],"EC":[29],"1.13.11.2]":[30],"encoded":[31],"by":[32,147,190],"the":[33,117,120,150,166,184,206],"Pseudomonas":[34],"putida":[35,204],"TOL":[36],"plasmid":[37,48,217],"xylE.":[39,88],"was":[42],"transferred":[43],"aB.":[45],"subtilis/Escherichia":[46],"coli":[47,102],"vector":[49],"construct":[51],"pTG402.":[52],"Although":[53],"xylE":[54,107,129,160,175],"functionally":[56],"expressed":[57],"E.":[59,101,200],"coli,":[60,201],"CatO2ase":[61,148,195],"not":[63],"detected":[64],"B.":[66,94,111,179,198],"unless":[68],"a":[69,81,141,222],"fragment":[70],"capable":[73],"promoting":[75],"ligated":[79],"into":[80],"cleavage":[82],"site":[83,169,186],"pTG402":[85],"upstream":[86],"from":[87,93],"Fragments":[89],"chromosomal":[91],"subtilis,":[95,199],"licheniformis,":[97],"pumilus,":[99],"and":[100,170,202],"are":[103,137],"shown":[104],"subtilis.":[112],"special":[114],"feature":[115],"detection:":[123],"colonies":[124],"cells":[126],"express":[128],"become":[130],"yellow":[131,151],"within":[132],"seconds":[133],"after":[134],"selection":[135],"plates":[136],"sprayed":[138],"with":[139],"catechol,":[140],"colorless":[142],"substrate":[143],"converted":[146],"product,":[152],"2-hydroxymuconic":[153],"semialdehyde.":[154],"complete":[156],"nucleotide":[157],"sequence":[158],"presented.":[162],"Strong":[163],"complementarity":[164],"between":[165],"ribosome":[167],"binding":[168],"16S":[171],"rRNA":[172],"suggests":[173],"mRNA":[176],"translation":[177],"may":[181],"commence":[182],"at":[183],"same":[185],"as":[187],"recognized":[189],"P.":[191,203],"putida.":[192],"Identity":[193],"produced":[196],"support":[205],"hypothesis.":[207],"Our":[208],"sensitive":[209],"color":[210],"assay":[211],"offers":[212],"an":[213],"approach":[214],"develop":[216],"vectors":[220],"for":[221],"wide":[223],"variety":[224],"host":[226],"organisms.":[227]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}