{"id":"https://openalex.org/W1966414797","doi":"https://doi.org/10.1016/s0092-8674(88)90489-8","title":"Purification and visualization of native spliceosomes","display_name":"Purification and visualization of native spliceosomes","publication_year":1988,"publication_date":"1988-06-01","ids":{"openalex":"https://openalex.org/W1966414797","doi":"https://doi.org/10.1016/s0092-8674(88)90489-8","mag":"1966414797","pmid":"https://pubmed.ncbi.nlm.nih.gov/2968159"},"language":"en","primary_location":{"id":"doi:10.1016/s0092-8674(88)90489-8","doi":"https://doi.org/10.1016/s0092-8674(88)90489-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0092-8674(88)90489-8","pdf_url":null,"source":{"id":"https://openalex.org/S110447773","display_name":"Cell","issn_l":"0092-8674","issn":["0092-8674","1097-4172"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315673","host_organization_name":"Cell Press","host_organization_lineage":["https://openalex.org/P4310315673","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Cell Press","Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038314229","display_name":"Robin Reed","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robin Reed","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061660001","display_name":"Jack D. Griffith","orcid":"https://orcid.org/0000-0002-8357-8689","observed_orcids":["https://orcid.org/0000-0002-8357-8689"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jack Griffith","raw_affiliation_strings":["Lineberger Cancer Research Center and Department of Microbiology and Immunology University of North Carolina Medical School Chapel Hill, North Carolina 27514, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lineberger Cancer Research Center and Department of Microbiology and Immunology University of North Carolina Medical School Chapel Hill, North Carolina 27514, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091116725","display_name":"Tom Maniatis","orcid":"https://orcid.org/0000-0002-2722-8633","observed_orcids":["https://orcid.org/0000-0002-2722-8633"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tom Maniatis","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology Harvard University 7 Divinity Avenue Cambridge, Massachusetts 02138, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology Harvard University 7 Divinity Avenue Cambridge, Massachusetts 02138, USA","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":11400,"currency":"USD","value_usd":11400},"apc_paid":null,"fwci":6.0148,"has_fulltext":false,"cited_by_count":114,"citation_normalized_percentile":{"value":0.97273948,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"53","issue":"6","first_page":"949","last_page":"961"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/spliceosome","display_name":"spliceosome","score":0.9290000200271606},{"id":"https://openalex.org/keywords/small-nuclear-ribonucleoproteins","display_name":"small nuclear ribonucleoproteins","score":0.7450000047683716},{"id":"https://openalex.org/keywords/u1-snrnp","display_name":"U1 snRNP","score":0.8389999866485596},{"id":"https://openalex.org/keywords/small-nuclear-rna","display_name":"small nuclear RNA","score":0.7129999995231628},{"id":"https://openalex.org/keywords/pre-mrna-splicing","display_name":"pre-mRNA splicing","score":0.9229999780654907},{"id":"https://openalex.org/keywords/spliceosome-assembly","display_name":"spliceosome assembly","score":0.6029999852180481},{"id":"https://openalex.org/keywords/micrococcal-nuclease-sensitivity","display_name":"micrococcal nuclease sensitivity","score":0.8009999990463257},{"id":"https://openalex.org/keywords/in-vitro-complementation","display_name":"in vitro complementation","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C40000846","wikidata":"https://www.wikidata.org/wiki/Q915868","display_name":"Spliceosome","level":5,"score":0.9500652551651001},{"id":"https://openalex.org/C68483431","wikidata":"https://www.wikidata.org/wiki/Q2076770","display_name":"snRNP","level":5,"score":0.8952960968017578},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7840741872787476},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.6387384533882141},{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.5708608031272888},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5170817375183105},{"id":"https://openalex.org/C139024627","wikidata":"https://www.wikidata.org/wiki/Q4293132","display_name":"Micrococcal nuclease","level":5,"score":0.4913152754306793},{"id":"https://openalex.org/C102063271","wikidata":"https://www.wikidata.org/wiki/Q7243183","display_name":"Precursor mRNA","level":5,"score":0.44832178950309753},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.4465581178665161},{"id":"https://openalex.org/C100299639","wikidata":"https://www.wikidata.org/wiki/Q284578","display_name":"Small nuclear RNA","level":5,"score":0.4294772744178772},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.38646063208580017},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3724815845489502},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.33937227725982666},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.20616701245307922},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18328648805618286},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.13096806406974792},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.09042441844940186},{"id":"https://openalex.org/C194993378","wikidata":"https://www.wikidata.org/wiki/Q427087","display_name":"Non-coding RNA","level":4,"score":0.08534196019172668},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06886911392211914},{"id":"https://openalex.org/C84772758","wikidata":"https://www.wikidata.org/wiki/Q271794","display_name":"Nucleosome","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002458","descriptor_name":"Cell Fractionation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005615","descriptor_name":"Freezing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005816","descriptor_name":"Genetic Complementation Test","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007118","descriptor_name":"Immunoassay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012261","descriptor_name":"Ribonucleoproteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012322","descriptor_name":"RNA Precursors","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D012326","descriptor_name":"RNA Splicing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D012342","descriptor_name":"RNA, Small Nuclear","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012342","descriptor_name":"RNA, Small Nuclear","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D017411","descriptor_name":"Ribonucleoproteins, Small Nuclear","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0092-8674(88)90489-8","doi":"https://doi.org/10.1016/s0092-8674(88)90489-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0092-8674(88)90489-8","pdf_url":null,"source":{"id":"https://openalex.org/S110447773","display_name":"Cell","issn_l":"0092-8674","issn":["0092-8674","1097-4172"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315673","host_organization_name":"Cell Press","host_organization_lineage":["https://openalex.org/P4310315673","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Cell Press","Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell","raw_type":"journal-article"},{"id":"pmid:2968159","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2968159","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1084920605","display_name":null,"funder_award_id":"HL27898","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G2774293502","display_name":null,"funder_award_id":"GM-31819","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306095","display_name":"American Cancer Society","ror":"https://ror.org/02e463172"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W52144977","https://openalex.org/W131136481","https://openalex.org/W177901988","https://openalex.org/W280710876","https://openalex.org/W1496392745","https://openalex.org/W1541258859","https://openalex.org/W1545060567","https://openalex.org/W1968322556","https://openalex.org/W1971314065","https://openalex.org/W1975642325","https://openalex.org/W1976644449","https://openalex.org/W1980424685","https://openalex.org/W1980428104","https://openalex.org/W1981587090","https://openalex.org/W1990823895","https://openalex.org/W1994261379","https://openalex.org/W2002944098","https://openalex.org/W2008422577","https://openalex.org/W2014551753","https://openalex.org/W2021783076","https://openalex.org/W2024588724","https://openalex.org/W2025975378","https://openalex.org/W2039762550","https://openalex.org/W2040539493","https://openalex.org/W2063550395","https://openalex.org/W2063749542","https://openalex.org/W2065104935","https://openalex.org/W2075362101","https://openalex.org/W2093200857","https://openalex.org/W2094972420","https://openalex.org/W2100179968","https://openalex.org/W2110090133","https://openalex.org/W2112590565","https://openalex.org/W2114992132","https://openalex.org/W2121813899","https://openalex.org/W2122071753","https://openalex.org/W2125959590","https://openalex.org/W2128635872","https://openalex.org/W2153635048","https://openalex.org/W2169345229","https://openalex.org/W2174405729","https://openalex.org/W3021597811","https://openalex.org/W4254469900","https://openalex.org/W6654117918","https://openalex.org/W7097080242"],"related_works":["https://openalex.org/W2162338462","https://openalex.org/W2043472165","https://openalex.org/W1984122961","https://openalex.org/W2012565885","https://openalex.org/W2076794781","https://openalex.org/W2061434726","https://openalex.org/W2118118220","https://openalex.org/W2010657933","https://openalex.org/W320630928","https://openalex.org/W2070301252"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}