{"id":"https://openalex.org/W1965819869","doi":"https://doi.org/10.1095/biolreprod46.5.912","title":"Qualitative Characterization of Oligosaccharide Chains Present on the Rat Zona Pellucida Glycoconjugates1","display_name":"Qualitative Characterization of Oligosaccharide Chains Present on the Rat Zona Pellucida Glycoconjugates1","publication_year":1992,"publication_date":"1992-05-01","ids":{"openalex":"https://openalex.org/W1965819869","doi":"https://doi.org/10.1095/biolreprod46.5.912","mag":"1965819869","pmid":"https://pubmed.ncbi.nlm.nih.gov/1591346"},"language":"en","primary_location":{"id":"doi:10.1095/biolreprod46.5.912","doi":"https://doi.org/10.1095/biolreprod46.5.912","is_oa":false,"landing_page_url":"https://doi.org/10.1095/biolreprod46.5.912","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051917586","display_name":"Yoshihiko Araki","orcid":"https://orcid.org/0000-0002-4759-9765","observed_orcids":["https://orcid.org/0000-0002-4759-9765"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoshihiko Araki Araki","raw_affiliation_strings":["Center for Reproductive Biology Research, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2633","Center for Reproductive Biology Research and Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232\u20132633"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Reproductive Biology Research, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2633","institution_ids":["https://openalex.org/I200719446"]},{"raw_affiliation_string":"Center for Reproductive Biology Research and Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232\u20132633","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110100370","display_name":"Marie\u2010Claire Orgebin\u2010Crist","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marie-Claire Orgebin-Crist","raw_affiliation_strings":["Center for Reproductive Biology Research and Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232\u20132633"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Reproductive Biology Research and Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232\u20132633","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113728681","display_name":"Daulat R.P. Tulsiani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Daulat Ram P. Tulsiani","raw_affiliation_strings":["Center for Reproductive Biology Research and Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232\u20132633"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Reproductive Biology Research and Department of Obstetrics & Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232\u20132633","institution_ids":["https://openalex.org/I200719446"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5113728681"],"corresponding_institution_ids":["https://openalex.org/I200719446"],"apc_list":{"value":4605,"currency":"USD","value_usd":4605},"apc_paid":null,"fwci":0.7822,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.66532957,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"46","issue":"5","first_page":"912","last_page":"919"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.8955000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.8955000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.08940000087022781,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/zona-pellucida","display_name":"zona pellucida","score":0.9919999837875366},{"id":"https://openalex.org/keywords/glycoconjugates","display_name":"glycoconjugates","score":0.8529999852180481},{"id":"https://openalex.org/keywords/oligosaccharide-chains","display_name":"oligosaccharide chains","score":0.9380000233650208},{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.7870000004768372},{"id":"https://openalex.org/keywords/o-glycosylation","display_name":"O-glycosylation","score":0.9990000128746033},{"id":"https://openalex.org/keywords/zp1","display_name":"ZP1","score":0.6769999861717224},{"id":"https://openalex.org/keywords/zp2","display_name":"ZP2","score":1.0},{"id":"https://openalex.org/keywords/zp3","display_name":"ZP3","score":0.9990000128746033},{"id":"https://openalex.org/keywords/rat-oocytes","display_name":"rat oocytes","score":0.6480000019073486},{"id":"https://openalex.org/keywords/sperm-egg-interaction","display_name":"sperm-egg interaction","score":0.7940000295639038},{"id":"https://openalex.org/keywords/endoglycosidase-f","display_name":"endoglycosidase F","score":0.8059999942779541},{"id":"https://openalex.org/keywords/n-glycanase","display_name":"N-glycanase","score":0.9409999847412109},{"id":"https://openalex.org/keywords/neuraminidase","display_name":"neuraminidase","score":0.8289999961853027},{"id":"https://openalex.org/keywords/alpha-l-fucosidase","display_name":"alpha-L-fucosidase","score":0.9440000057220459},{"id":"https://openalex.org/keywords/sialic-acid","display_name":"sialic acid","score":0.6069999933242798},{"id":"https://openalex.org/keywords/fucosyl-residues","display_name":"fucosyl residues","score":0.7929999828338623}],"concepts":[{"id":"https://openalex.org/C2776010925","wikidata":"https://www.wikidata.org/wiki/Q219841","display_name":"Zona pellucida","level":4,"score":0.9432188272476196},{"id":"https://openalex.org/C2780654058","wikidata":"https://www.wikidata.org/wiki/Q5376339","display_name":"Endoglycosidase","level":3,"score":0.924039363861084},{"id":"https://openalex.org/C2779571893","wikidata":"https://www.wikidata.org/wiki/Q24775767","display_name":"Endoglycosidase H","level":4,"score":0.7500635385513306},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7398982048034668},{"id":"https://openalex.org/C2910492233","wikidata":"https://www.wikidata.org/wiki/Q219841","display_name":"Zona pellucida glycoprotein","level":5,"score":0.7127594351768494},{"id":"https://openalex.org/C13426554","wikidata":"https://www.wikidata.org/wiki/Q416019","display_name":"Glycoconjugate","level":2,"score":0.6230447292327881},{"id":"https://openalex.org/C2781060939","wikidata":"https://www.wikidata.org/wiki/Q753104","display_name":"Castanospermine","level":3,"score":0.6011449694633484},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.5505014061927795},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5005538463592529},{"id":"https://openalex.org/C73695083","wikidata":"https://www.wikidata.org/wiki/Q409485","display_name":"Sialidase","level":4,"score":0.46716558933258057},{"id":"https://openalex.org/C134164806","wikidata":"https://www.wikidata.org/wiki/Q409485","display_name":"Neuraminidase","level":3,"score":0.4671575129032135},{"id":"https://openalex.org/C2777726330","wikidata":"https://www.wikidata.org/wiki/Q320607","display_name":"Oligosaccharide","level":2,"score":0.4658212661743164},{"id":"https://openalex.org/C206212055","wikidata":"https://www.wikidata.org/wiki/Q2553138","display_name":"Glycan","level":3,"score":0.4639871120452881},{"id":"https://openalex.org/C2776690073","wikidata":"https://www.wikidata.org/wiki/Q3246226","display_name":"Oocyte","level":3,"score":0.4503806233406067},{"id":"https://openalex.org/C2780098789","wikidata":"https://www.wikidata.org/wiki/Q898356","display_name":"Glycocalyx","level":2,"score":0.4372698664665222},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.30614709854125977},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.23797157406806946},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.23751205205917358},{"id":"https://openalex.org/C119062480","wikidata":"https://www.wikidata.org/wiki/Q83181","display_name":"Golgi apparatus","level":3,"score":0.098320871591568},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.08427464962005615}],"mesh":[{"descriptor_ui":"D000072216","descriptor_name":"Zona Pellucida Glycoproteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004527","descriptor_name":"Egg Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011919","descriptor_name":"Rats, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011956","descriptor_name":"Receptors, Cell Surface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015044","descriptor_name":"Zona Pellucida","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1095/biolreprod46.5.912","doi":"https://doi.org/10.1095/biolreprod46.5.912","is_oa":false,"landing_page_url":"https://doi.org/10.1095/biolreprod46.5.912","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},{"id":"pmid:1591346","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1591346","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of reproduction","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5111909320","display_name":"MAMMALIAN SPERM SURFACE MANNOSIDASE","funder_award_id":"R01HD025869","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G6168058362","display_name":null,"funder_award_id":"HD-03820","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"funders":[{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2057589247","https://openalex.org/W2003521113","https://openalex.org/W2015777045","https://openalex.org/W2003914055","https://openalex.org/W1996380673","https://openalex.org/W2588792149","https://openalex.org/W2025356775","https://openalex.org/W2021431729","https://openalex.org/W2135515001","https://openalex.org/W1529389226"],"abstract_inverted_index":{"Zona":[0],"pellucida":[1],"(ZP),":[2],"the":[3,7,35,41,45,54,58,73,77,92,142,162,195,209,238,246],"extracellular":[4],"glycocalyx":[5],"surrounding":[6],"mammalian":[8],"oocyte,":[9],"is":[10,32],"believed":[11],"to":[12,180,221],"mediate":[13],"species-specific":[14],"sperm-egg":[15],"interaction.":[16],"Despite":[17],"numerous":[18],"studies":[19,89],"on":[20,34,83,124,245],"characterization":[21,56],"of":[22,40,44,57,96,103,141,194,240,250],"ZP":[23,60,79,94,144,211,226],"glycoconjugates":[24],"in":[25,133,192],"several":[26],"species,":[27],"little":[28],"or":[29,242],"no":[30],"information":[31],"available":[33],"number":[36],"and":[37,76,110,117,128,137,149,158,168,183,198,204],"chemical":[38],"nature":[39],"various":[42],"components":[43,80,98,123,145,200,212],"rat":[46,59,93,126,143,166,196,251],"ZP.":[47],"In":[48],"this":[49,225],"study":[50],"we":[51],"have":[52],"attempted":[53],"biochemical":[55],"using":[61],"endo-":[62,152],"and/or":[63,151],"exo-glycohydrolases.":[64],"Intact":[65],"eggs":[66],"from":[67],"superovulated":[68],"rats":[69],"were":[70,81],"radioiodinated":[71],"by":[72,177,201],"chloramine-T":[74],"method,":[75],"labeled":[78],"resolved":[82],"SDS-PAGE":[84],"under":[85],"nonreducing":[86],"conditions.":[87],"These":[88],"show":[90,130],"that":[91,208,224],"consists":[95],"three":[97],"with":[99,146,187],"apparent":[100],"molecular":[101,135],"masses":[102],"205":[104],"kDa":[105,108,112],"(ZP1),":[106],"119":[107],"(ZP2),":[109],"115":[111],"(ZP3).":[113],"Unlike":[114],"mouse":[115],"ZP2":[116,127,167,197],"ZP3,":[118],"which":[119],"resolve":[120],"as":[121,175],"distinct":[122],"SDS-PAGE,":[125],"ZP3":[129,169,199,218],"substantial":[131],"overlap":[132],"their":[134,178],"sizes":[136],"isoelectric":[138],"points.":[139],"Treatment":[140,186],"exo-":[147],"(neuraminidase":[148],"alpha-L-fucosidase)":[150],"(endoglycosidase":[153],"H,":[154],"endoglycosidase":[155,181],"F,":[156],"N-glycanase,":[157],"O-glycanase)":[159],"glycohydrolases":[160],"indicated":[161,176],"following:":[163],"1)":[164],"Both":[165],"contain":[170],"N-linked":[171],"oligosaccharide":[172],"(OS)":[173],"units":[174],"sensitivity":[179],"F":[182],"N-glycanase.":[184],"2)":[185],"N-glycanase":[188],"caused":[189],"a":[190],"reduction":[191],"size":[193],"nearly":[202],"50%":[203],"60%,":[205],"respectively,":[206],"indicating":[207],"two":[210],"are":[213],"highly":[214],"glycosylated.":[215],"3)":[216],"Rat":[217],"was":[219,235],"sensitive":[220],"O-glycanase,":[222],"suggesting":[223],"component":[227],"contains":[228],"O-linked":[229,247],"OS":[230,248],"unit(s).":[231],"4)":[232],"No":[233],"evidence":[234],"obtained":[236],"for":[237],"presence":[239],"fucosyl":[241],"sialyl":[243],"residue(s)":[244],"unit(s)":[249],"ZP3.(ABSTRACT":[252],"TRUNCATED":[253],"AT":[254],"250":[255],"WORDS)":[256]},"counts_by_year":[{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}