{"id":"https://openalex.org/W1965740863","doi":"https://doi.org/10.1006/mthe.2002.0713","title":"The Organotypic Multicellular Spheroid Is a Relevant Three-Dimensional Model to Study Adenovirus Replication and Penetration in Human Tumors in Vitro","display_name":"The Organotypic Multicellular Spheroid Is a Relevant Three-Dimensional Model to Study Adenovirus Replication and Penetration in Human Tumors in Vitro","publication_year":2002,"publication_date":"2002-11-01","ids":{"openalex":"https://openalex.org/W1965740863","doi":"https://doi.org/10.1006/mthe.2002.0713","mag":"1965740863","pmid":"https://pubmed.ncbi.nlm.nih.gov/12409259"},"language":"en","primary_location":{"id":"doi:10.1006/mthe.2002.0713","doi":"https://doi.org/10.1006/mthe.2002.0713","is_oa":true,"landing_page_url":"https://doi.org/10.1006/mthe.2002.0713","pdf_url":"http://www.cell.com/article/S1525001602907136/pdf","source":{"id":"https://openalex.org/S51596929","display_name":"Molecular Therapy","issn_l":"1525-0016","issn":["1525-0016","1525-0024"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Therapy","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.cell.com/article/S1525001602907136/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048911676","display_name":"Jacques Grill","orcid":"https://orcid.org/0000-0003-4773-3001","observed_orcids":["https://orcid.org/0000-0003-4773-3001","https://orcid.org/0000-0001-9937-0180","https://orcid.org/0009-0003-9578-8821"]},"institutions":[{"id":"https://openalex.org/I2801843802","display_name":"Institut Gustave Roussy","ror":"https://ror.org/0321g0743","country_code":"FR","type":"facility","lineage":["https://openalex.org/I2801843802"]},{"id":"https://openalex.org/I4405269973","display_name":"Fondation Gustave Roussy","ror":"https://ror.org/05rfnt234","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4405269973"]},{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["FR","NL"],"is_corresponding":false,"raw_author_name":"Jacques Grill","raw_affiliation_strings":["Department of Pediatric Oncology, Gustave Roussy Institute, Villejuif cedex, 94805, France","Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pediatric Oncology, Gustave Roussy Institute, Villejuif cedex, 94805, France","institution_ids":["https://openalex.org/I2801843802","https://openalex.org/I4405269973"]},{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042649357","display_name":"Martine L.M. Lamfers","orcid":"https://orcid.org/0000-0001-7745-9029","observed_orcids":["https://orcid.org/0000-0001-7745-9029"]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"Martine L.M. Lamfers","raw_affiliation_strings":["Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]},{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108504728","display_name":"Victor W. van Beusechem","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Victor W. van Beusechem","raw_affiliation_strings":["Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063092847","display_name":"Clemens M.F. Dirven","orcid":"https://orcid.org/0000-0002-0713-2722","observed_orcids":["https://orcid.org/0000-0002-0713-2722"]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Clemens M. Dirven","raw_affiliation_strings":["Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]},{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048974113","display_name":"D.Shareen Pherai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"D.Shareen Pherai","raw_affiliation_strings":["Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]},{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086060821","display_name":"Mathijs Kater","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Mathijs Kater","raw_affiliation_strings":["Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]},{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103252713","display_name":"Paul van der Valk","orcid":"https://orcid.org/0000-0002-0983-1607","observed_orcids":["https://orcid.org/0000-0002-0983-1607"]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Paul Van der Valk","raw_affiliation_strings":["Department of Pathology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053114102","display_name":"Ronald Vogels","orcid":"https://orcid.org/0009-0007-0818-6160","observed_orcids":["https://orcid.org/0009-0007-0818-6160"]},"institutions":[{"id":"https://openalex.org/I4210157709","display_name":"Johnson & Johnson (Netherlands)","ror":"https://ror.org/04vkhtf23","country_code":"NL","type":"company","lineage":["https://openalex.org/I1330063522","https://openalex.org/I4210157709"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Ronald Vogels","raw_affiliation_strings":["Crucell Holland BV, Leiden, 2301 CA, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Crucell Holland BV, Leiden, 2301 CA, The Netherlands","institution_ids":["https://openalex.org/I4210157709"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004632162","display_name":"William Peter Vandertop","orcid":"https://orcid.org/0000-0001-5417-0265","observed_orcids":["https://orcid.org/0000-0001-5417-0265"]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"W.Peter Vandertop","raw_affiliation_strings":["Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105878379","display_name":"Herbert M. Pinedo","orcid":"https://orcid.org/0000-0002-8980-371X","observed_orcids":["https://orcid.org/0000-0002-8980-371X"]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Herbert M. Pinedo","raw_affiliation_strings":["Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022987623","display_name":"David T. Curiel","orcid":"https://orcid.org/0000-0003-3802-6014","observed_orcids":["https://orcid.org/0000-0003-3802-6014"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]},{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL","US"],"is_corresponding":false,"raw_author_name":"David T. Curiel","raw_affiliation_strings":["Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","Gene Therapy Center, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]},{"raw_affiliation_string":"Gene Therapy Center, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039709555","display_name":"Winald R. Gerritsen","orcid":"https://orcid.org/0000-0003-1110-9119","observed_orcids":["https://orcid.org/0000-0003-1110-9119"]},"institutions":[{"id":"https://openalex.org/I911458345","display_name":"Amsterdam UMC Location Vrije Universiteit Amsterdam","ror":"https://ror.org/00q6h8f30","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210151833","https://openalex.org/I911458345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Winald R. Gerritsen","raw_affiliation_strings":["Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Gene Therapy, Department of Medical Oncology, VU University Medical Center, Amsterdam, 1007 MB, The Netherlands","institution_ids":["https://openalex.org/I911458345"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5042649357"],"corresponding_institution_ids":["https://openalex.org/I911458345"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":{"value":4500,"currency":"USD","value_usd":4500},"fwci":2.3298,"has_fulltext":false,"cited_by_count":61,"citation_normalized_percentile":{"value":0.87539187,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"6","issue":"5","first_page":"609","last_page":"614"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12996","display_name":"Bacterial Cancer Therapy","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adenovirus","display_name":"adenovirus","score":0.8349999785423279},{"id":"https://openalex.org/keywords/3d-spheroids","display_name":"multicellular spheroids","score":0.8360000252723694},{"id":"https://openalex.org/keywords/organotypic-culture","display_name":"organotypic culture","score":0.6980000138282776},{"id":"https://openalex.org/keywords/three-dimensional-tumor-models","display_name":"3D tumor models","score":0.8500000238418579},{"id":"https://openalex.org/keywords/oncolytic-adenovirus","display_name":"oncolytic adenovirus","score":0.7979999780654907},{"id":"https://openalex.org/keywords/viral-replication","display_name":"viral replication","score":0.6060000061988831},{"id":"https://openalex.org/keywords/virus-entry","display_name":"viral entry","score":0.8100000023841858},{"id":"https://openalex.org/keywords/brain-tumors","display_name":"brain tumors","score":0.7239999771118164},{"id":"https://openalex.org/keywords/gene-transfer","display_name":"gene transfer","score":0.49900001287460327},{"id":"https://openalex.org/keywords/luciferase-reporter","display_name":"luciferase reporter","score":0.7310000061988831},{"id":"https://openalex.org/keywords/lacz-reporter","display_name":"lacZ reporter","score":0.9229999780654907},{"id":"https://openalex.org/keywords/oncolysis","display_name":"oncolysis","score":0.6690000295639038}],"concepts":[{"id":"https://openalex.org/C82210918","wikidata":"https://www.wikidata.org/wiki/Q1560099","display_name":"Oncolytic virus","level":3,"score":0.9048376083374023},{"id":"https://openalex.org/C2777283488","wikidata":"https://www.wikidata.org/wiki/Q7095181","display_name":"Oncolytic adenovirus","level":4,"score":0.7600370645523071},{"id":"https://openalex.org/C175369904","wikidata":"https://www.wikidata.org/wiki/Q208395","display_name":"Spheroid","level":3,"score":0.7242758870124817},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6958162188529968},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.5816829800605774},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5733152031898499},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.5716507434844971},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5207933783531189},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.517876386642456},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.4777854382991791},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4671083688735962},{"id":"https://openalex.org/C49744831","wikidata":"https://www.wikidata.org/wiki/Q600121","display_name":"Virotherapy","level":4,"score":0.43372052907943726},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.42226266860961914},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4130077362060547},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4105949401855469},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3823869228363037},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.36112141609191895},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.354770302772522},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.3486858606338501},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.27761584520339966},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.15751540660858154},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09853518009185791},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001932","descriptor_name":"Brain Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D013778","descriptor_name":"Tetrazolium Salts","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018874","descriptor_name":"Spheroids, Cellular","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1006/mthe.2002.0713","doi":"https://doi.org/10.1006/mthe.2002.0713","is_oa":true,"landing_page_url":"https://doi.org/10.1006/mthe.2002.0713","pdf_url":"http://www.cell.com/article/S1525001602907136/pdf","source":{"id":"https://openalex.org/S51596929","display_name":"Molecular Therapy","issn_l":"1525-0016","issn":["1525-0016","1525-0024"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Therapy","raw_type":"journal-article"},{"id":"pmid:12409259","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12409259","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular therapy : the journal of the American Society of Gene Therapy","raw_type":"Journal Article"},{"id":"pmh:oai:pure.amsterdamumc.nl:openaire_cris_publications/1258106e-5b9c-4d6b-a46b-4bfc58c862a0","doi":null,"is_oa":false,"landing_page_url":"https://pure.amsterdamumc.nl/en/publications/1258106e-5b9c-4d6b-a46b-4bfc58c862a0","pdf_url":null,"source":{"id":"https://openalex.org/S7407055222","display_name":"Pure Amsterdam UMC","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Grill, J, Lamfers, M L M, van Beusechem, V W, Dirven, C M, Pherai, D S, Kater, M, Van der Valk, P, Vogels, R, Vandertop, W P, Pinedo, H M, Curiel, D T & Gerritsen, W R 2002, 'The organotypic multicellular spheroid is a relevant three-dimensional model to study adenovirus replication and penetration in human tumors in vitro', Molecular therapy, vol. 6, no. 5, pp. 609-14.","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/dd15800e-660a-43ac-b258-eb1abb28a3cf","doi":null,"is_oa":false,"landing_page_url":"https://research.vumc.nl/en/publications/dd15800e-660a-43ac-b258-eb1abb28a3cf","pdf_url":null,"source":{"id":"https://openalex.org/S7407055350","display_name":"Amsterdam UMC (VU Amsterdam) - Institutional Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Grill, J, Lamfers, M L M, van Beusechem, V W, Dirven, C M, Pherai, D S, Kater, M, Van der Valk, P, Vogels, R, Vandertop, W P, Pinedo, H M, Curiel, D T & Gerritsen, W R 2002, 'The organotypic multicellular spheroid is a relevant three-dimensional model to study adenovirus replication and penetration in human tumors in vitro', Molecular Therapy, vol. 6, no. 5, pp. 609-14.","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:vumc:oai:pure.atira.dk:publications/dd15800e-660a-43ac-b258-eb1abb28a3cf","doi":null,"is_oa":false,"landing_page_url":"https://research.vumc.nl/en/publications/dd15800e-660a-43ac-b258-eb1abb28a3cf","pdf_url":null,"source":{"id":"https://openalex.org/S4306401843","display_name":"Data Archiving and Networked Services (DANS)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1322597698","host_organization_name":"Royal Netherlands Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/I1322597698"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Molecular Therapy, 6(5), 609 - 14. Nature Publishing Group","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1006/mthe.2002.0713","doi":"https://doi.org/10.1006/mthe.2002.0713","is_oa":true,"landing_page_url":"https://doi.org/10.1006/mthe.2002.0713","pdf_url":"http://www.cell.com/article/S1525001602907136/pdf","source":{"id":"https://openalex.org/S51596929","display_name":"Molecular Therapy","issn_l":"1525-0016","issn":["1525-0016","1525-0024"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Therapy","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9074000120162964,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2589755068","display_name":"ADENOVIRAL MEDIATED TARGETED GENE DELIVERY","funder_award_id":"R01CA074242","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G2732183576","display_name":null,"funder_award_id":"R01 HLDK-50277","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G2888511834","display_name":"GENE THERAPY FOR OVARIAN CANCER","funder_award_id":"R01CA068245","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W1965740863.pdf"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W1544229812","https://openalex.org/W2001070008","https://openalex.org/W2005517346","https://openalex.org/W2018390208","https://openalex.org/W2023062664","https://openalex.org/W2034234959","https://openalex.org/W2041585886","https://openalex.org/W2051326848","https://openalex.org/W2052582425","https://openalex.org/W2082305396","https://openalex.org/W2088292624","https://openalex.org/W2089906021","https://openalex.org/W2100405920","https://openalex.org/W2101957677","https://openalex.org/W2107985871","https://openalex.org/W2117520087","https://openalex.org/W2119859866","https://openalex.org/W2138710378","https://openalex.org/W2142058281","https://openalex.org/W2154369685","https://openalex.org/W2406551180","https://openalex.org/W6681143239"],"related_works":["https://openalex.org/W4232296147","https://openalex.org/W2101957677","https://openalex.org/W2024662453","https://openalex.org/W2074520970","https://openalex.org/W2249690945","https://openalex.org/W2178296037","https://openalex.org/W4211071717","https://openalex.org/W4240619052","https://openalex.org/W3009982287","https://openalex.org/W2410852615"],"abstract_inverted_index":{"The":[0],"use":[1],"of":[2,46,85,132,135],"adenoviruses":[3,55,157],"for":[4,34,150],"gene":[5],"transfer":[6],"and":[7,22,54,67,106,154],"as":[8],"oncolytic":[9,130],"agents":[10],"is":[11,31],"currently":[12],"receiving":[13],"widespread":[14],"attention.":[15],"As":[16],"specific":[17],"constraints":[18],"to":[19,81,127],"adenovirus":[20,90],"distribution":[21],"spread":[23],"cannot":[24],"be":[25,125],"studied":[26,49],"in":[27,36,43,100,147,158],"cell":[28],"cultures,":[29],"there":[30],"a":[32,88,119,145,159],"need":[33],"an":[35],"vitro":[37,148],"three-dimensional":[38],"(3D)":[39],"model":[40],"mimicking":[41],"the":[42,50,82,92,101,112,129],"vivo":[44],"biology":[45],"tumors.":[47],"We":[48,138],"interactions":[51],"between":[52],"tumor":[53,63],"using":[56],"multicellular":[57],"spheroids":[58,143],"grown":[59],"from":[60],"primary":[61],"brain":[62],"material.":[64],"Using":[65,87],"beta-galactosidase":[66],"luciferase":[68,93],"reporter":[69],"genes":[70],"expressed":[71],"by":[72,156],"replication-defective":[73],"adenoviruses,":[74],"we":[75,95],"showed":[76,96],"that":[77,97,107,141],"infection":[78],"was":[79,103],"restricted":[80],"first":[83],"layer":[84],"cells.":[86],"replication-competent":[89,136],"expressing":[91],"gene,":[94],"transgene":[98],"expression":[99],"spheroid":[102],"considerably":[104],"enhanced":[105],"viral":[108],"spreading":[109],"deep":[110],"into":[111],"3D":[113],"structure":[114],"took":[115],"place.":[116],"In":[117],"addition,":[118],"tetrazolium":[120],"salt-based":[121],"metabolic":[122],"assay":[123],"could":[124],"used":[126],"compare":[128],"activity":[131],"different":[133],"concentrations":[134],"adenoviruses.":[137],"can":[139],"conclude":[140],"organotypic":[142],"offer":[144],"versatile":[146],"system":[149],"studying":[151],"distribution,":[152],"spread,":[153],"oncolysis":[155],"clinically":[160],"relevant":[161],"model.":[162]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}