{"id":"https://openalex.org/W1965733735","doi":"https://doi.org/10.1016/0166-0934(93)90104-y","title":"A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens","display_name":"A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens","publication_year":1993,"publication_date":"1993-12-01","ids":{"openalex":"https://openalex.org/W1965733735","doi":"https://doi.org/10.1016/0166-0934(93)90104-y","mag":"1965733735","pmid":"https://pubmed.ncbi.nlm.nih.gov/8113346"},"language":"en","primary_location":{"id":"doi:10.1016/0166-0934(93)90104-y","doi":"https://doi.org/10.1016/0166-0934(93)90104-y","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0166-0934(93)90104-y","pdf_url":null,"source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/20.500.12792/3135","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017617705","display_name":"Gustavo Nolasco","orcid":"https://orcid.org/0000-0002-7025-6023","observed_orcids":["https://orcid.org/0000-0002-7025-6023"]},"institutions":[{"id":"https://openalex.org/I4210106474","display_name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","ror":"https://ror.org/011q66e29","country_code":"ES","type":"government","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210106474"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"G. Nolasco","raw_affiliation_strings":["Departamento Protecci\u00f3n Vegetal, CIT-INIA, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento Protecci\u00f3n Vegetal, CIT-INIA, Madrid, Spain","institution_ids":["https://openalex.org/I4210106474"]}]},{"author_position":"middle","author":{"id":null,"display_name":"C. De Blas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210106474","display_name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","ror":"https://ror.org/011q66e29","country_code":"ES","type":"government","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210106474"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"C. De Blas","raw_affiliation_strings":["Departamento Protecci\u00f3n Vegetal, CIT-INIA, Apartado 8111, 28080 MadridSpain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento Protecci\u00f3n Vegetal, CIT-INIA, Apartado 8111, 28080 MadridSpain","institution_ids":["https://openalex.org/I4210106474"]}]},{"author_position":"middle","author":{"id":null,"display_name":"V. Torres","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210106474","display_name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","ror":"https://ror.org/011q66e29","country_code":"ES","type":"government","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210106474"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"V. Torres","raw_affiliation_strings":["Departamento Protecci\u00f3n Vegetal, CIT-INIA, Apartado 8111, 28080 MadridSpain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento Protecci\u00f3n Vegetal, CIT-INIA, Apartado 8111, 28080 MadridSpain","institution_ids":["https://openalex.org/I4210106474"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084144306","display_name":"F Ponz","orcid":"https://orcid.org/0000-0003-0344-4363","observed_orcids":["https://orcid.org/0000-0003-0344-4363"]},"institutions":[{"id":"https://openalex.org/I4210106474","display_name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","ror":"https://ror.org/011q66e29","country_code":"ES","type":"government","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210106474"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"F. Ponz","raw_affiliation_strings":["Departamento Protecci\u00f3n Vegetal, CIT-INIA, Apartado 8111, 28080 MadridSpain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento Protecci\u00f3n Vegetal, CIT-INIA, Apartado 8111, 28080 MadridSpain","institution_ids":["https://openalex.org/I4210106474"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5084144306"],"corresponding_institution_ids":["https://openalex.org/I4210106474"],"apc_list":{"value":3210,"currency":"USD","value_usd":3210},"apc_paid":null,"fwci":6.4168,"has_fulltext":false,"cited_by_count":211,"citation_normalized_percentile":{"value":0.96346853,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"45","issue":"2","first_page":"201","last_page":"218"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13220","display_name":"Plant and Fungal Interactions Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plant-virus-detection","display_name":"plant virus detection","score":0.9369999766349792},{"id":"https://openalex.org/keywords/subviral-pathogens","display_name":"subviral pathogens","score":0.9210000038146973},{"id":"https://openalex.org/keywords/viroids","display_name":"viroids","score":0.8769999742507935},{"id":"https://openalex.org/keywords/satellite-rna","display_name":"satellite RNA","score":0.9649999737739563},{"id":"https://openalex.org/keywords/immunocapture","display_name":"immunocapture","score":0.9559999704360962},{"id":"https://openalex.org/keywords/rt-pcr","display_name":"RT-PCR","score":0.8519999980926514},{"id":"https://openalex.org/keywords/microtiter-plate-assay","display_name":"microtiter plate assay","score":0.8949999809265137},{"id":"https://openalex.org/keywords/bean-yellow-mosaic-virus","display_name":"bean yellow mosaic virus","score":0.875},{"id":"https://openalex.org/keywords/cucumber-mosaic-virus","display_name":"cucumber mosaic virus","score":0.9919999837875366},{"id":"https://openalex.org/keywords/citrus-tristeza-virus","display_name":"citrus tristeza virus","score":0.9909999966621399},{"id":"https://openalex.org/keywords/grapevine-fanleaf-virus","display_name":"grapevine fanleaf virus","score":0.9980000257492065},{"id":"https://openalex.org/keywords/potato-leafroll-virus","display_name":"potato leafroll virus","score":0.9950000047683716},{"id":"https://openalex.org/keywords/pepper-mild-mottle-virus","display_name":"pepper mild mottle virus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/tomato-spotted-wilt-virus","display_name":"tomato spotted wilt virus","score":0.9890000224113464},{"id":"https://openalex.org/keywords/potato-spindle-tuber-viroid","display_name":"potato spindle tuber viroid","score":0.9670000076293945}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8354806900024414},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7603155374526978},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.7602437734603882},{"id":"https://openalex.org/C2778580959","wikidata":"https://www.wikidata.org/wiki/Q882236","display_name":"Potato leafroll virus","level":4,"score":0.6610569357872009},{"id":"https://openalex.org/C2776513250","wikidata":"https://www.wikidata.org/wiki/Q1518692","display_name":"Cucumber mosaic virus","level":4,"score":0.6321351528167725},{"id":"https://openalex.org/C109110057","wikidata":"https://www.wikidata.org/wiki/Q1495434","display_name":"Plant virus","level":3,"score":0.5054662227630615},{"id":"https://openalex.org/C4692481","wikidata":"https://www.wikidata.org/wiki/Q169526","display_name":"Reverse transcription polymerase chain reaction","level":4,"score":0.4853779077529907},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.47998473048210144},{"id":"https://openalex.org/C144707691","wikidata":"https://www.wikidata.org/wiki/Q332874","display_name":"Tobacco mosaic virus","level":3,"score":0.4755142331123352},{"id":"https://openalex.org/C2777642389","wikidata":"https://www.wikidata.org/wiki/Q2414314","display_name":"Potato virus X","level":4,"score":0.448709100484848},{"id":"https://openalex.org/C2779174946","wikidata":"https://www.wikidata.org/wiki/Q751911","display_name":"RNA virus","level":4,"score":0.4105727970600128},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13338258862495422},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.10301095247268677},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000914","descriptor_name":"Antibodies, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010942","descriptor_name":"Plant Viruses","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010942","descriptor_name":"Plant Viruses","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010942","descriptor_name":"Plant Viruses","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014773","descriptor_name":"Virology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D014773","descriptor_name":"Virology","qualifier_ui":"Q000706","qualifier_name":"statistics & numerical data","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000706","qualifier_name":"statistics & numerical data","is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1016/0166-0934(93)90104-y","doi":"https://doi.org/10.1016/0166-0934(93)90104-y","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0166-0934(93)90104-y","pdf_url":null,"source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},{"id":"pmid:8113346","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8113346","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virological methods","raw_type":"Journal Article"},{"id":"pmh:oai:digital.csic.es:10261/292388","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10261/292388","pdf_url":null,"source":{"id":"https://openalex.org/S4306400616","display_name":"DIGITAL.CSIC (Spanish National Research Council (CSIC))","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I134820265","host_organization_name":"Consejo Superior de Investigaciones Cient\u00edficas","host_organization_lineage":["https://openalex.org/I134820265"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"art\u00edculo"},{"id":"pmh:oai:dnet:r2i2________::76c00b60a2540258da7142dcf3273a86","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.12792/3135","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:sapientia.ualg.pt:10400.1/6224","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10400.1/6224","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"journal article"}],"best_oa_location":{"id":"pmh:oai:dnet:r2i2________::76c00b60a2540258da7142dcf3273a86","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.12792/3135","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.41260001063346863}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310009","display_name":"University of Florida","ror":"https://ror.org/02y3ad647"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W63003135","https://openalex.org/W1589680365","https://openalex.org/W1772424160","https://openalex.org/W1969893775","https://openalex.org/W1969942361","https://openalex.org/W1971941732","https://openalex.org/W1972046136","https://openalex.org/W1972934647","https://openalex.org/W1973473614","https://openalex.org/W1983892719","https://openalex.org/W1986803448","https://openalex.org/W1992920804","https://openalex.org/W1997405570","https://openalex.org/W2003431268","https://openalex.org/W2024954683","https://openalex.org/W2025233870","https://openalex.org/W2026437342","https://openalex.org/W2036484042","https://openalex.org/W2047546869","https://openalex.org/W2048005830","https://openalex.org/W2050717506","https://openalex.org/W2050961711","https://openalex.org/W2054282979","https://openalex.org/W2062913104","https://openalex.org/W2067758591","https://openalex.org/W2070201579","https://openalex.org/W2071121656","https://openalex.org/W2082579798","https://openalex.org/W2087666605","https://openalex.org/W2090487254","https://openalex.org/W2095176950","https://openalex.org/W2098104967","https://openalex.org/W2104259684","https://openalex.org/W2117295257","https://openalex.org/W2129931786","https://openalex.org/W2144634347","https://openalex.org/W2158206240","https://openalex.org/W2166037777","https://openalex.org/W2167575871","https://openalex.org/W2167921568","https://openalex.org/W2168978266","https://openalex.org/W2314225431","https://openalex.org/W2401817009","https://openalex.org/W2409086233","https://openalex.org/W6757686404","https://openalex.org/W7098124190"],"related_works":["https://openalex.org/W2154088640","https://openalex.org/W2010529665","https://openalex.org/W600164049","https://openalex.org/W2613982797","https://openalex.org/W1039560223","https://openalex.org/W2077683182","https://openalex.org/W298236017","https://openalex.org/W2057643260","https://openalex.org/W2157592471","https://openalex.org/W3030069633"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":8},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":9},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}