{"id":"https://openalex.org/W1965315699","doi":"https://doi.org/10.1002/elps.200410042","title":"Protein polymer drag\u2010tags for DNA separations by end\u2010labeled free\u2010solution electrophoresis","display_name":"Protein polymer drag\u2010tags for DNA separations by end\u2010labeled free\u2010solution electrophoresis","publication_year":2005,"publication_date":"2005-05-09","ids":{"openalex":"https://openalex.org/W1965315699","doi":"https://doi.org/10.1002/elps.200410042","mag":"1965315699","pmid":"https://pubmed.ncbi.nlm.nih.gov/15880624"},"language":"en","primary_location":{"id":"doi:10.1002/elps.200410042","doi":"https://doi.org/10.1002/elps.200410042","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.200410042","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010152085","display_name":"Jong\u2010In Won","orcid":"https://orcid.org/0000-0002-3133-4236","observed_orcids":["https://orcid.org/0000-0002-3133-4236","https://orcid.org/0009-0006-3821-8293"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jong\u2010In Won","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020091525","display_name":"Robert J. Meagher","orcid":"https://orcid.org/0000-0002-6262-2126","observed_orcids":["https://orcid.org/0000-0002-6262-2126"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert J. Meagher","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA","Department of Chemical & Biological Engineering Northwestern University  Evanston IL USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA","institution_ids":["https://openalex.org/I111979921"]},{"raw_affiliation_string":"Department of Chemical & Biological Engineering Northwestern University  Evanston IL USA","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013082584","display_name":"Annelise E. Barron","orcid":"https://orcid.org/0000-0002-0735-6873","observed_orcids":["https://orcid.org/0000-0002-0735-6873"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Annelise E. Barron","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA","Northwestern University, Department of Chemical and Biological Engineering, 2145 Sheridan Road, Rm. E136, Evanston, IL 60208, USA Fax: +847\u2010491\u20103728"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA","institution_ids":["https://openalex.org/I111979921"]},{"raw_affiliation_string":"Northwestern University, Department of Chemical and Biological Engineering, 2145 Sheridan Road, Rm. E136, Evanston, IL 60208, USA Fax: +847\u2010491\u20103728","institution_ids":["https://openalex.org/I111979921"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5013082584"],"corresponding_institution_ids":["https://openalex.org/I111979921"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":2.1001,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.84207731,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"26","issue":"11","first_page":"2138","last_page":"2148"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11582","display_name":"Nanopore and Nanochannel Transport Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-separation","display_name":"DNA separation","score":0.8209999799728394},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.5720000267028809},{"id":"https://openalex.org/keywords/electrophoretic-mobility","display_name":"electrophoretic mobility","score":0.7089999914169312},{"id":"https://openalex.org/keywords/microchannel-electrophoresis","display_name":"microchannel electrophoresis","score":0.746999979019165},{"id":"https://openalex.org/keywords/streptavidin","display_name":"streptavidin","score":0.6499999761581421},{"id":"https://openalex.org/keywords/skin-friction","display_name":"skin friction","score":0.6069999933242798}],"concepts":[{"id":"https://openalex.org/C72921944","wikidata":"https://www.wikidata.org/wiki/Q206621","display_name":"Drag","level":2,"score":0.8236435055732727},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.764083743095398},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.7454240918159485},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6249880194664001},{"id":"https://openalex.org/C2900643","wikidata":"https://www.wikidata.org/wiki/Q747468","display_name":"Capillary electrophoresis","level":2,"score":0.5906357765197754},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5881036520004272},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4697589874267578},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.4652588963508606},{"id":"https://openalex.org/C11432220","wikidata":"https://www.wikidata.org/wiki/Q1975334","display_name":"Dispersity","level":2,"score":0.44422805309295654},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23232528567314148},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.17321768403053284},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12351691722869873},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.09652653336524963},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008968","descriptor_name":"Molecular Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015335","descriptor_name":"Molecular Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046209","descriptor_name":"Electrophoresis, Microchip","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/elps.200410042","doi":"https://doi.org/10.1002/elps.200410042","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.200410042","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:15880624","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15880624","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8995062146","display_name":"DNA sequencing by ELFSE on microfluidic devices","funder_award_id":"R01HG002918","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W159732665","https://openalex.org/W1965062979","https://openalex.org/W1970781522","https://openalex.org/W1976332548","https://openalex.org/W1987423375","https://openalex.org/W1992237760","https://openalex.org/W2017017495","https://openalex.org/W2033713657","https://openalex.org/W2044912124","https://openalex.org/W2054123045","https://openalex.org/W2056080046","https://openalex.org/W2068580333","https://openalex.org/W2070325613","https://openalex.org/W2075312336","https://openalex.org/W2093972722","https://openalex.org/W2144524487","https://openalex.org/W2158896328","https://openalex.org/W2165235672"],"related_works":["https://openalex.org/W2094168251","https://openalex.org/W2128773567","https://openalex.org/W2102157799","https://openalex.org/W1993518446","https://openalex.org/W2051754524","https://openalex.org/W2234047260","https://openalex.org/W2107169352","https://openalex.org/W2060165205","https://openalex.org/W7098803652","https://openalex.org/W2154781427"],"abstract_inverted_index":{"We":[0,154,238],"demonstrate":[1],"the":[2,76,89,93,103,108,116,156,162,167,199,220],"feasibility":[3],"of":[4,10,59,75,92,102,107,119,158,188,219,234],"end-labeled":[5],"free-solution":[6,97],"electrophoresis":[7,99],"(ELFSE)":[8],"separation":[9],"DNA":[11,83,124,180,212],"using":[12,217],"genetically":[13],"engineered":[14],"protein":[15,77,109,148,221],"polymers":[16,20,39,78],"as":[17,259,265],"drag-tags.":[18],"Protein":[19],"are":[21,32],"promising":[22],"candidates":[23],"for":[24,51,179,203],"ELFSE":[25,204],"drag-tags":[26,111,134,176,196,223],"because":[27],"their":[28],"sequences":[29],"and":[30,68,87,105,127,130,150,165,194,206],"lengths":[31],"controllable":[33],"not":[34],"only":[35],"to":[36,46,79,132,146,210],"generate":[37],"monodisperse":[38],"with":[40,230],"high":[41],"frictional":[42,249],"drag,":[43],"but":[44],"also":[45,239],"meet":[47],"other":[48],"drag-tag":[49,267],"requirements":[50],"high-resolution":[52],"separations":[53],"by":[54,96,137,161],"microchannel":[55],"electrophoresis.":[56],"A":[57],"series":[58],"repetitive":[60],"polypeptides":[61],"was":[62,135],"designed,":[63],"expressed":[64],"in":[65,182],"Escherichia":[66],"coli,":[67],"purified.":[69],"By":[70],"performing":[71],"an":[72],"end-on":[73],"conjugation":[74],"a":[80,231,266],"fluorescently":[81,139],"labeled":[82,140],"oligomer":[84],"(22":[85],"bases)":[86,129],"analyzing":[88,151],"electrophoretic":[90,117],"mobilities":[91],"conjugate":[94],"molecules":[95],"capillary":[98],"(CE),":[100],"effects":[101],"size":[104],"charge":[106],"polymer":[110,222],"were":[112,177],"investigated.":[113],"In":[114],"addition,":[115],"behavior":[118],"bioconjugates":[120],"comprising":[121],"relatively":[122],"long":[123],"fragments":[125],"(108":[126],"208":[128],"attached":[131],"uncharged":[133,195],"observed,":[136],"conjugating":[138],"polymerase":[141],"chain":[142,232],"reaction":[143],"(PCR)":[144],"products":[145],"charge-neutral":[147],"polymers,":[149],"via":[152],"CE.":[153],"calculated":[155],"amount":[157],"friction":[159],"generated":[160],"various":[163],"drag-tags,":[164],"estimated":[166],"potential":[168],"read-lengths":[169],"that":[170,192,207,241],"could":[171,214],"be":[172,215],"obtained":[173],"if":[174],"these":[175,189],"used":[178,264],"sequencing":[181],"our":[183],"current":[184],"system.":[185],"The":[186],"results":[187],"studies":[190],"indicate":[191],"larger":[193],"will":[197],"have":[198],"best":[200],"DNA-resolving":[201],"capability":[202],"separations,":[205],"theoretically,":[208],"up":[209],"233":[211],"bases":[213],"sequenced":[216],"one":[218],"we":[224],"produced,":[225],"which":[226,261],"is":[227],"electrostatically":[228],"neutral":[229],"length":[233],"337":[235],"amino":[236],"acids.":[237],"show":[240],"denatured":[242],"(unfolded)":[243],"polypeptide":[244],"chains":[245],"impose":[246],"much":[247],"greater":[248],"drag":[250],"per":[251],"unit":[252],"molecular":[253],"weight":[254],"than":[255],"folded":[256],"proteins,":[257],"such":[258],"streptavidin,":[260],"has":[262],"been":[263],"before.":[268]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}