{"id":"https://openalex.org/W1964953277","doi":"https://doi.org/10.1074/jbc.273.45.29519","title":"Differential Effects of Hydroxyurea upon Deoxyribonucleoside Triphosphate Pools, Analyzed with Vaccinia Virus Ribonucleotide Reductase","display_name":"Differential Effects of Hydroxyurea upon Deoxyribonucleoside Triphosphate Pools, Analyzed with Vaccinia Virus Ribonucleotide Reductase","publication_year":1998,"publication_date":"1998-11-01","ids":{"openalex":"https://openalex.org/W1964953277","doi":"https://doi.org/10.1074/jbc.273.45.29519","mag":"1964953277","pmid":"https://pubmed.ncbi.nlm.nih.gov/9792659"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.273.45.29519","doi":"https://doi.org/10.1074/jbc.273.45.29519","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.45.29519","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.273.45.29519","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014544239","display_name":"Stephen P. Hendricks","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen P. Hendricks","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-7305, USA","Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, 97331-7305"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-7305, USA","institution_ids":["https://openalex.org/I131249849"]},{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, 97331-7305","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113535189","display_name":"Christopher K. Mathews","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Christopher K. Mathews","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-7305"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-7305","institution_ids":["https://openalex.org/I131249849"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5113535189"],"corresponding_institution_ids":["https://openalex.org/I131249849"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.5507,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.59709917,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"273","issue":"45","first_page":"29519","last_page":"29523"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydroxyurea","display_name":"hydroxyurea","score":1.0},{"id":"https://openalex.org/keywords/ribonucleotide-reductase","display_name":"ribonucleotide reductase","score":0.8989999890327454},{"id":"https://openalex.org/keywords/vaccinia-virus","display_name":"vaccinia virus","score":0.925000011920929},{"id":"https://openalex.org/keywords/allosteric-regulation","display_name":"allosteric regulation","score":0.6320000290870667},{"id":"https://openalex.org/keywords/dna-synthesis-inhibition","display_name":"DNA synthesis inhibition","score":0.7570000290870667},{"id":"https://openalex.org/keywords/holoenzyme","display_name":"holoenzyme","score":0.675000011920929}],"concepts":[{"id":"https://openalex.org/C4710235","wikidata":"https://www.wikidata.org/wiki/Q420089","display_name":"Ribonucleotide reductase","level":4,"score":0.9699699282646179},{"id":"https://openalex.org/C2776123222","wikidata":"https://www.wikidata.org/wiki/Q28751","display_name":"Deoxyribonucleoside","level":3,"score":0.8007370233535767},{"id":"https://openalex.org/C2776844798","wikidata":"https://www.wikidata.org/wiki/Q34402","display_name":"Ribonucleoside","level":4,"score":0.6500998735427856},{"id":"https://openalex.org/C194101687","wikidata":"https://www.wikidata.org/wiki/Q583813","display_name":"Nucleotide salvage","level":4,"score":0.5470028519630432},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5375757813453674},{"id":"https://openalex.org/C2781356689","wikidata":"https://www.wikidata.org/wiki/Q1986297","display_name":"Vaccinia","level":4,"score":0.5364644527435303},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.49199411273002625},{"id":"https://openalex.org/C98892162","wikidata":"https://www.wikidata.org/wiki/Q422484","display_name":"Ribonucleotide","level":4,"score":0.4758555591106415},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.47557106614112854},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.4661864936351776},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.46089616417884827},{"id":"https://openalex.org/C2910443878","wikidata":"https://www.wikidata.org/wiki/Q28751","display_name":"Deoxyribonucleotides","level":4,"score":0.4208069145679474},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36319220066070557},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.14782023429870605},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.09872928261756897},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.06633025407791138},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D003854","descriptor_name":"Deoxyribonucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006918","descriptor_name":"Hydroxyurea","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D012264","descriptor_name":"Ribonucleotide Reductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014616","descriptor_name":"Vaccinia virus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.273.45.29519","doi":"https://doi.org/10.1074/jbc.273.45.29519","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.45.29519","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:9792659","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9792659","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.273.45.29519","doi":"https://doi.org/10.1074/jbc.273.45.29519","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.45.29519","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2728278873","display_name":null,"funder_award_id":"GM-55234","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W91354526","https://openalex.org/W1488625988","https://openalex.org/W1505281346","https://openalex.org/W1514207813","https://openalex.org/W1569161839","https://openalex.org/W1603488046","https://openalex.org/W1636387388","https://openalex.org/W1670233224","https://openalex.org/W1833536387","https://openalex.org/W1881743461","https://openalex.org/W1947036774","https://openalex.org/W1966538841","https://openalex.org/W1967830103","https://openalex.org/W1973435750","https://openalex.org/W1976410676","https://openalex.org/W2001236884","https://openalex.org/W2029473646","https://openalex.org/W2058563502","https://openalex.org/W2066552121","https://openalex.org/W2066969026","https://openalex.org/W2072620686","https://openalex.org/W2084814230","https://openalex.org/W2092089811","https://openalex.org/W2107918972","https://openalex.org/W2170473454","https://openalex.org/W2413229695","https://openalex.org/W2415213076"],"related_works":["https://openalex.org/W1881743461","https://openalex.org/W2415213076","https://openalex.org/W2052601376","https://openalex.org/W2097357480","https://openalex.org/W1538415171","https://openalex.org/W1988528612","https://openalex.org/W1551180964","https://openalex.org/W1976410676","https://openalex.org/W1576560493","https://openalex.org/W2001236884"],"abstract_inverted_index":{"Hydroxyurea":[0],"inhibits":[1],"DNA":[2],"synthesis":[3,23],"by":[4,32,81,101,196],"destroying":[5],"the":[6,20,42,49,60,76,112,116,126,131,140,147,150,163,169,174],"catalytically":[7],"essential":[8],"free":[9,72,164],"radical":[10,73,135,156,181],"of":[11,24,41,51,62,71,90,111,118,134,142,149,192],"class":[12],"I":[13],"ribonucleoside":[14],"diphosphate":[15],"(rNDP)":[16],"reductase,":[17,80],"thereby":[18],"blocking":[19],"de":[21],"novo":[22],"deoxyribonucleotides.":[25],"In":[26,59],"mammalian":[27],"cells,":[28],"including":[29],"those":[30],"infected":[31],"vaccinia":[33,77],"virus,":[34],"hydroxyurea":[35,93],"treatment":[36],"causes":[37],"a":[38,88,103],"differential":[39],"depletion":[40,191],"four":[43,113],"deoxyribonucleoside":[44],"triphosphate":[45,153],"pools,":[46],"suggesting":[47],"that":[48,107,124],"activities":[50],"rNDP":[52,79],"reductase":[53],"are":[54,185],"differentially":[55],"sensitive":[56],"to":[57,137,168,187],"hydroxyurea.":[58,197],"presence":[61,117],"different":[63],"substrates":[64,119],"and":[65,120],"allosteric":[66,143],"modifiers,":[67],"we":[68,96],"measured":[69],"rates":[70,160],"destruction":[74],"in":[75,115],"virus-coded":[78],"following":[82],"absorbance":[83],"at":[84,122],"417":[85],"nm":[86],"as":[87],"function":[89],"time":[91],"after":[92],"addition.":[94],"Also,":[95],"followed":[97],"enzyme":[98],"activity":[99,148],"directly,":[100],"using":[102],"recently":[104],"developed":[105],"assay":[106],"allows":[108],"simultaneous":[109],"monitoring":[110],"activities,":[114],"effectors":[121,154],"concentrations":[123],"approximate":[125],"intracellular":[127],"environment.":[128],"We":[129],"found":[130],"primary":[132],"determinant":[133],"loss":[136],"be":[138],"not":[139],"ensemble":[141],"ligands":[144],"bound":[145],"but":[146],"enzyme.":[151,165],"Nucleoside":[152],"accelerated":[155,180],"decay,":[157],"compared":[158],"with":[159,162],"seen":[161],"Adding":[166],"substrate":[167],"holoenzyme,":[170],"under":[171],"conditions":[172],"where":[173],"enzymatic":[175],"reaction":[176],"is":[177],"proceeding,":[178],"further":[179],"decay.":[182],"Alternative":[183],"models":[184],"discussed,":[186],"account":[188],"for":[189],"selective":[190],"purine":[193],"nucleotide":[194],"pools":[195]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}