{"id":"https://openalex.org/W1964790718","doi":"https://doi.org/10.1021/bm501546k","title":"Cysi\u2013Lysi+3\u2013Lysi+4 Triad: A General Approach for PEG-Based Stabilization of \u03b1-Helical Proteins","display_name":"Cysi\u2013Lysi+3\u2013Lysi+4 Triad: A General Approach for PEG-Based Stabilization of \u03b1-Helical Proteins","publication_year":2014,"publication_date":"2014-11-11","ids":{"openalex":"https://openalex.org/W1964790718","doi":"https://doi.org/10.1021/bm501546k","mag":"1964790718","pmid":"https://pubmed.ncbi.nlm.nih.gov/25387132"},"language":"en","primary_location":{"id":"doi:10.1021/bm501546k","doi":"https://doi.org/10.1021/bm501546k","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bm501546k","pdf_url":null,"source":{"id":"https://openalex.org/S11629336","display_name":"Biomacromolecules","issn_l":"1525-7797","issn":["1525-7797","1526-4602"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomacromolecules","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Brijesh K. Pandey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brijesh K. Pandey","raw_affiliation_strings":["Department of Chemistry and\rBiochemistry, Brigham Young University, Provo, Utah 84602, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and\rBiochemistry, Brigham Young University, Provo, Utah 84602, United States","institution_ids":["https://openalex.org/I100005738"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077387378","display_name":"M.S. Smith","orcid":"https://orcid.org/0000-0002-3772-143X","observed_orcids":["https://orcid.org/0000-0002-3772-143X"]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mason S. Smith","raw_affiliation_strings":["Department of Chemistry and\rBiochemistry, Brigham Young University, Provo, Utah 84602, United States","Department of Chemistry and#R#Biochemistry, Brigham Young University,#R#Provo, Utah 84602, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and\rBiochemistry, Brigham Young University, Provo, Utah 84602, United States","institution_ids":["https://openalex.org/I100005738"]},{"raw_affiliation_string":"Department of Chemistry and#R#Biochemistry, Brigham Young University,#R#Provo, Utah 84602, United States","institution_ids":["https://openalex.org/I100005738"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081564025","display_name":"Joshua L. Price","orcid":"https://orcid.org/0000-0002-0116-0968","observed_orcids":["https://orcid.org/0000-0002-0116-0968"]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joshua L. Price","raw_affiliation_strings":["Department of Chemistry and\rBiochemistry, Brigham Young University, Provo, Utah 84602, United States","Department of Chemistry and#R#Biochemistry, Brigham Young University,#R#Provo, Utah 84602, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and\rBiochemistry, Brigham Young University, Provo, Utah 84602, United States","institution_ids":["https://openalex.org/I100005738"]},{"raw_affiliation_string":"Department of Chemistry and#R#Biochemistry, Brigham Young University,#R#Provo, Utah 84602, United States","institution_ids":["https://openalex.org/I100005738"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5081564025"],"corresponding_institution_ids":["https://openalex.org/I100005738"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.4098,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.58635909,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"15","issue":"12","first_page":"4643","last_page":"4647"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.7348999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.7348999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.13130000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.07169999927282333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pegylation","display_name":"PEGylation","score":0.9800000190734863},{"id":"https://openalex.org/keywords/protein-stabilization","display_name":"protein stabilization","score":0.6069999933242798},{"id":"https://openalex.org/keywords/alpha-helical-proteins","display_name":"alpha-helical proteins","score":0.8199999928474426},{"id":"https://openalex.org/keywords/coiled-coil","display_name":"coiled coil","score":0.871999979019165},{"id":"https://openalex.org/keywords/conformational-stability","display_name":"conformational stability","score":0.8830000162124634}],"concepts":[{"id":"https://openalex.org/C2779149719","wikidata":"https://www.wikidata.org/wiki/Q682252","display_name":"PEGylation","level":3,"score":0.9280529618263245},{"id":"https://openalex.org/C2780105360","wikidata":"https://www.wikidata.org/wiki/Q6743002","display_name":"Maleimide","level":2,"score":0.7710801362991333},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7605726718902588},{"id":"https://openalex.org/C55988834","wikidata":"https://www.wikidata.org/wiki/Q903650","display_name":"Coiled coil","level":2,"score":0.7042137384414673},{"id":"https://openalex.org/C54400483","wikidata":"https://www.wikidata.org/wiki/Q1793202","display_name":"PEG ratio","level":2,"score":0.6204412579536438},{"id":"https://openalex.org/C176038584","wikidata":"https://www.wikidata.org/wiki/Q626432","display_name":"Triad (sociology)","level":2,"score":0.5956340432167053},{"id":"https://openalex.org/C2778530040","wikidata":"https://www.wikidata.org/wiki/Q1937315","display_name":"Helix (gastropod)","level":3,"score":0.5206798315048218},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.48630914092063904},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.44641315937042236},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4427562654018402},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.3485279679298401},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3305298686027527},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17400813102722168},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.1565835177898407},{"id":"https://openalex.org/C2776964284","wikidata":"https://www.wikidata.org/wiki/Q410083","display_name":"Polyethylene glycol","level":2,"score":0.12262803316116333},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.09671956300735474},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C10138342","wikidata":"https://www.wikidata.org/wiki/Q43015","display_name":"Finance","level":1,"score":0.0},{"id":"https://openalex.org/C2779965526","wikidata":"https://www.wikidata.org/wiki/Q308841","display_name":"Snail","level":2,"score":0.0},{"id":"https://openalex.org/C11171543","wikidata":"https://www.wikidata.org/wiki/Q41630","display_name":"Psychoanalysis","level":1,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bm501546k","doi":"https://doi.org/10.1021/bm501546k","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bm501546k","pdf_url":null,"source":{"id":"https://openalex.org/S11629336","display_name":"Biomacromolecules","issn_l":"1525-7797","issn":["1525-7797","1526-4602"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomacromolecules","raw_type":"journal-article"},{"id":"pmid:25387132","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25387132","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomacromolecules","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320309309","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W1490299254","https://openalex.org/W1659323555","https://openalex.org/W1766185368","https://openalex.org/W1964641915","https://openalex.org/W1973897761","https://openalex.org/W1975251855","https://openalex.org/W1992726856","https://openalex.org/W2003451283","https://openalex.org/W2007088212","https://openalex.org/W2018473603","https://openalex.org/W2022234371","https://openalex.org/W2036350738","https://openalex.org/W2039839088","https://openalex.org/W2048049691","https://openalex.org/W2052061962","https://openalex.org/W2055290772","https://openalex.org/W2063258624","https://openalex.org/W2087777969","https://openalex.org/W2098353657","https://openalex.org/W2100513413","https://openalex.org/W2130939465","https://openalex.org/W2139616692","https://openalex.org/W2146029618","https://openalex.org/W2154794547","https://openalex.org/W2163553212","https://openalex.org/W2314876208","https://openalex.org/W2315456628","https://openalex.org/W2321067616","https://openalex.org/W2326149775","https://openalex.org/W2329902654","https://openalex.org/W2496025240"],"related_works":["https://openalex.org/W2512641548","https://openalex.org/W2328777086","https://openalex.org/W2588663839","https://openalex.org/W2316754003","https://openalex.org/W2056042015","https://openalex.org/W2763822422","https://openalex.org/W7023646273","https://openalex.org/W7052143647","https://openalex.org/W2405350396","https://openalex.org/W2148458061"],"abstract_inverted_index":{"PEGylation":[0,40,48,68],"is":[1],"an":[2,56],"important":[3],"strategy":[4,141],"for":[5,37,142],"enhancing":[6],"the":[7,45,50,85,99,102,114,118,131,135],"pharmacokinetic":[8],"properties":[9],"of":[10,20,47,53,69,117,133,145],"protein":[11,29],"drugs.":[12],"Modern":[13],"chemoselective":[14],"reactions":[15],"now":[16],"enable":[17],"specific":[18],"placement":[19],"a":[21,28,61,70,110,139],"single":[22],"PEG":[23],"at":[24],"any":[25],"site":[26],"on":[27,49],"surface.":[30],"However,":[31],"few":[32],"rational":[33],"structure-based":[34],"guidelines":[35],"exist":[36],"selecting":[38],"optimal":[39],"sites.":[41],"Here,":[42],"we":[43],"explore":[44],"impact":[46],"conformational":[51,128],"stability":[52],"\u03b1-helices":[54],"using":[55,134],"\u03b1-helical":[57],"coiled":[58],"coil":[59],"as":[60,138],"model":[62],"system.":[63],"We":[64],"find":[65],"that":[66],"maleimide-based":[67],"solvent-exposed":[71,111],"i":[72,86,90],"position":[73,112],"Cys":[74],"can":[75],"stabilize":[76],"coiled-coil":[77],"quaternary":[78],"structure":[79],"when":[80],"Lys":[81,103],"residues":[82],"occupy":[83],"both":[84],"+":[87,91],"3":[88],"and":[89,101],"4":[92],"positions,":[93],"due":[94],"to":[95,109,123],"favorable":[96],"interactions":[97],"between":[98],"PEG-maleimide":[100],"residues.":[104],"Applying":[105],"this":[106],"Cys(i)-Lys(i+3)-Lys(i+4)":[107,136],"triad":[108,137],"within":[113],"C-terminal":[115],"helix":[116],"villin":[119],"headpiece":[120],"domain":[121],"leads":[122],"similar":[124],"PEG-based":[125,143],"increases":[126],"in":[127],"stability,":[129],"highlighting":[130],"possibility":[132],"general":[140],"stabilization":[144],"helical":[146],"proteins.":[147]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}