{"id":"https://openalex.org/W1964768202","doi":"https://doi.org/10.1089/cbr.2010.0766","title":"Generation and Characterization of Non-Small-Cell Lung Cancer Cell Lines and Clones for Use in the Study of Immunotherapy","display_name":"Generation and Characterization of Non-Small-Cell Lung Cancer Cell Lines and Clones for Use in the Study of Immunotherapy","publication_year":2010,"publication_date":"2010-06-01","ids":{"openalex":"https://openalex.org/W1964768202","doi":"https://doi.org/10.1089/cbr.2010.0766","mag":"1964768202","pmid":"https://pubmed.ncbi.nlm.nih.gov/20578832"},"language":"en","primary_location":{"id":"doi:10.1089/cbr.2010.0766","doi":"https://doi.org/10.1089/cbr.2010.0766","is_oa":false,"landing_page_url":"https://doi.org/10.1089/cbr.2010.0766","pdf_url":null,"source":{"id":"https://openalex.org/S87235987","display_name":"Cancer Biotherapy and Radiopharmaceuticals","issn_l":"1084-9785","issn":["1084-9785","1557-8852"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Biotherapy and Radiopharmaceuticals","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109093980","display_name":"John R. Yannelli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I143302722","display_name":"University of Kentucky","ror":"https://ror.org/02k3smh20","country_code":"US","type":"education","lineage":["https://openalex.org/I143302722"]},{"id":"https://openalex.org/I2802239656","display_name":"Markey Cancer Center","ror":"https://ror.org/01dhvva97","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285210727","https://openalex.org/I143302722","https://openalex.org/I2802239656"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John R. Yannelli","raw_affiliation_strings":["University of Kentucky","Department of Microbiology, Immunology and Molecular Genetics and the Markey Cancer Center, University of Kentucky, Lexington, Kentucky"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Kentucky","institution_ids":["https://openalex.org/I143302722"]},{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics and the Markey Cancer Center, University of Kentucky, Lexington, Kentucky","institution_ids":["https://openalex.org/I143302722","https://openalex.org/I2802239656"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5109093980"],"corresponding_institution_ids":["https://openalex.org/I143302722","https://openalex.org/I2802239656"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"25","issue":"3","first_page":"269","last_page":"278"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12334","display_name":"Lung Cancer Research Studies","score":0.5746999979019165,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12334","display_name":"Lung Cancer Research Studies","score":0.5746999979019165,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.15719999372959137,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.0681999996304512,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/non-small-cell-lung-cancer","display_name":"non-small cell lung cancer","score":0.9990000128746033},{"id":"https://openalex.org/keywords/cancer-cell-lines","display_name":"cancer cell lines","score":0.9459999799728394},{"id":"https://openalex.org/keywords/cell-line-establishment","display_name":"cell line establishment","score":0.6690000295639038},{"id":"https://openalex.org/keywords/adenocarcinoma","display_name":"adenocarcinoma","score":0.8759999871253967},{"id":"https://openalex.org/keywords/squamous-cell-carcinoma","display_name":"squamous cell carcinoma","score":1.0},{"id":"https://openalex.org/keywords/limiting-dilution-analysis","display_name":"limiting dilution analysis","score":0.6349999904632568},{"id":"https://openalex.org/keywords/antigen-expression","display_name":"antigen expression","score":0.6620000004768372},{"id":"https://openalex.org/keywords/cytokine-production","display_name":"cytokine production","score":0.9139999747276306},{"id":"https://openalex.org/keywords/cell-mediated-immunity","display_name":"cell-mediated immunity","score":0.7480000257492065},{"id":"https://openalex.org/keywords/fibroblast-contamination","display_name":"fibroblast contamination","score":0.675000011920929},{"id":"https://openalex.org/keywords/culture-medium","display_name":"culture media","score":0.4860000014305115}],"concepts":[{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6408847570419312},{"id":"https://openalex.org/C2777701055","wikidata":"https://www.wikidata.org/wiki/Q1427096","display_name":"Immunotherapy","level":3,"score":0.621930718421936},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5959709882736206},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5755125284194946},{"id":"https://openalex.org/C2781182431","wikidata":"https://www.wikidata.org/wiki/Q356033","display_name":"Adenocarcinoma","level":3,"score":0.550190806388855},{"id":"https://openalex.org/C2776256026","wikidata":"https://www.wikidata.org/wiki/Q47912","display_name":"Lung cancer","level":2,"score":0.5385445356369019},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5301460027694702},{"id":"https://openalex.org/C2780868878","wikidata":"https://www.wikidata.org/wiki/Q1660197","display_name":"Immunogenicity","level":3,"score":0.5083310008049011},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4908193051815033},{"id":"https://openalex.org/C2780674031","wikidata":"https://www.wikidata.org/wiki/Q2012719","display_name":"Cancer immunotherapy","level":4,"score":0.4546436071395874},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4487242102622986},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.4339427947998047},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.4126797020435333},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.40708938241004944},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.32079949975013733},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.29989293217658997},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.06799209117889404}],"mesh":[{"descriptor_ui":"D000230","descriptor_name":"Adenocarcinoma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D000951","descriptor_name":"Antigens, Neoplasm","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000951","descriptor_name":"Antigens, Neoplasm","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002289","descriptor_name":"Carcinoma, Non-Small-Cell Lung","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D002289","descriptor_name":"Carcinoma, Non-Small-Cell Lung","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":false},{"descriptor_ui":"D002294","descriptor_name":"Carcinoma, Squamous Cell","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007371","descriptor_name":"Interferon-gamma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":false},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008214","descriptor_name":"Lymphocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008214","descriptor_name":"Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D013602","descriptor_name":"T-Lymphocytes, Cytotoxic","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D013602","descriptor_name":"T-Lymphocytes, Cytotoxic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015395","descriptor_name":"Histocompatibility Antigens Class I","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016233","descriptor_name":"Immunotherapy, Active","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016753","descriptor_name":"Interleukin-10","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018122","descriptor_name":"B7-1 Antigen","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018920","descriptor_name":"Coculture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019496","descriptor_name":"Cancer Vaccines","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053773","descriptor_name":"Transforming Growth Factor beta1","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1089/cbr.2010.0766","doi":"https://doi.org/10.1089/cbr.2010.0766","is_oa":false,"landing_page_url":"https://doi.org/10.1089/cbr.2010.0766","pdf_url":null,"source":{"id":"https://openalex.org/S87235987","display_name":"Cancer Biotherapy and Radiopharmaceuticals","issn_l":"1084-9785","issn":["1084-9785","1557-8852"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Biotherapy and Radiopharmaceuticals","raw_type":"journal-article"},{"id":"pmid:20578832","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20578832","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer biotherapy & radiopharmaceuticals","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W205399872","https://openalex.org/W1492737592","https://openalex.org/W1498829233","https://openalex.org/W1517338238","https://openalex.org/W1965706136","https://openalex.org/W1970472201","https://openalex.org/W1974700255","https://openalex.org/W1981793279","https://openalex.org/W1998284666","https://openalex.org/W1998681197","https://openalex.org/W1999060144","https://openalex.org/W2007085833","https://openalex.org/W2021821919","https://openalex.org/W2029152630","https://openalex.org/W2029621076","https://openalex.org/W2030879320","https://openalex.org/W2054841768","https://openalex.org/W2063517932","https://openalex.org/W2067583381","https://openalex.org/W2067761086","https://openalex.org/W2074600271","https://openalex.org/W2076792998","https://openalex.org/W2077137895","https://openalex.org/W2082751482","https://openalex.org/W2109048628","https://openalex.org/W2124430059","https://openalex.org/W2125506721","https://openalex.org/W2126427337","https://openalex.org/W2128222730","https://openalex.org/W2134640829","https://openalex.org/W2140633397","https://openalex.org/W2142540954","https://openalex.org/W2143899065","https://openalex.org/W2146571367","https://openalex.org/W2149489144","https://openalex.org/W2157535803","https://openalex.org/W2228808639","https://openalex.org/W4242948142"],"related_works":["https://openalex.org/W1976298147","https://openalex.org/W150652571","https://openalex.org/W2153534888","https://openalex.org/W4403692330","https://openalex.org/W3000034067","https://openalex.org/W2336738197","https://openalex.org/W2300788244","https://openalex.org/W1999060144","https://openalex.org/W2241946219","https://openalex.org/W1970686660"],"abstract_inverted_index":{"The":[0,178],"in":[1,44,75,106,158,166,189],"vitro":[2],"study":[3,23,117,205],"of":[4,13,40,60,71,121,162,168,206],"cancer":[5,52],"has":[6],"been":[7],"made":[8],"easier":[9],"by":[10,85,101],"the":[11,37,107,111,119,173,203,207],"use":[12],"stable":[14,151],"tumor":[15,46],"cell":[16,42,76,125,137],"(TC)":[17],"lines":[18,43,126,186],"derived":[19],"from":[20,110,132],"patients":[21],"to":[22,29,68,89,148,175,211],"antigen":[24,170],"expression,":[25,171],"immunogenicity,":[26],"and":[27,32,127,135,143,172,180,195],"response":[28,210],"both":[30,133],"experimental":[31],"conventional":[33],"therapeutic":[34],"agents.":[35],"However,":[36],"routine":[38],"generation":[39],"these":[41,150],"some":[45,96,161],"histologies":[47],"such":[48],"as":[49,199],"non-small-cell":[50],"lung":[51],"(NSCLC)":[53],"is":[54],"difficult.":[55],"In":[56,95],"many":[57],"cases,":[58,97],"colonies":[59,82],"TCs":[61],"do":[62],"not":[63],"survive,":[64],"most":[65],"likely":[66],"due":[67],"a":[69],"lack":[70],"critical":[72,200],"growth":[73,93],"factors":[74],"culture":[77,141],"medium.":[78],"Other":[79],"times,":[80],"TC":[81,152,185],"are":[83,99,187],"overgrown":[84,100],"fibroblasts,":[86],"which":[87,163],"appear":[88],"have":[90],"less":[91],"stringent":[92],"requirements.":[94],"cultures":[98,194],"bacteria":[102],"or":[103,113],"mold":[104],"contained":[105],"biopsy":[108],"arriving":[109],"surgical":[112],"pathology":[114],"suite.":[115],"This":[116],"presents":[118],"characteristics":[120],"three":[122],"new":[123],"NSCLC":[124],"associated":[128,182],"autologous":[129],"clones":[130,197],"generated":[131],"adenocarcinoma":[134],"squamous":[136],"carcinoma":[138],"tissue.":[139],"Different":[140],"media":[142],"variable":[144],"techniques":[145],"were":[146],"used":[147],"generate":[149],"lines.":[153],"Limiting":[154],"dilution":[155],"analysis":[156],"resulted":[157],"numerous":[159],"clones,":[160],"displayed":[164],"heterogeneity":[165],"terms":[167],"growth,":[169],"ability":[174],"release":[176],"cytokines.":[177],"successes":[179],"failures":[181],"with":[183],"generating":[184],"discussed":[188],"this":[190],"article.":[191],"Both":[192],"parental":[193],"related":[196],"serve":[198],"reagents":[201],"for":[202],"continued":[204],"cellular":[208],"immune":[209],"NSCLC.":[212]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}