{"id":"https://openalex.org/W1964107343","doi":"https://doi.org/10.1074/jbc.m010290200","title":"Crystal Structure of Mannanase 26A from Pseudomonas cellulosa and Analysis of Residues Involved in Substrate Binding","display_name":"Crystal Structure of Mannanase 26A from Pseudomonas cellulosa and Analysis of Residues Involved in Substrate Binding","publication_year":2001,"publication_date":"2001-08-01","ids":{"openalex":"https://openalex.org/W1964107343","doi":"https://doi.org/10.1074/jbc.m010290200","mag":"1964107343","pmid":"https://pubmed.ncbi.nlm.nih.gov/11382747"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m010290200","doi":"https://doi.org/10.1074/jbc.m010290200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m010290200","pdf_url":"http://www.jbc.org/article/S0021925820802725/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925820802725/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017452466","display_name":"Deborah Hogg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Deborah Hogg","raw_affiliation_strings":["Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089432012","display_name":"Eui\u2010Jeon Woo","orcid":"https://orcid.org/0000-0001-6983-0270","observed_orcids":["https://orcid.org/0000-0001-6983-0270"]},"institutions":[{"id":"https://openalex.org/I166337079","display_name":"Queen Mary University of London","ror":"https://ror.org/026zzn846","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I166337079"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Eui-Jeon Woo","raw_affiliation_strings":["School of Biological Sciences, Medical Sciences Building, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, Medical Sciences Building, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom","institution_ids":["https://openalex.org/I166337079"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070568728","display_name":"David N. Bolam","orcid":"https://orcid.org/0000-0003-0314-3122","observed_orcids":["https://orcid.org/0000-0003-0314-3122"]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"David N. Bolam","raw_affiliation_strings":["Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom and the","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027173851","display_name":"Vincent A. McKie","orcid":"https://orcid.org/0000-0003-3348-5182","observed_orcids":["https://orcid.org/0000-0003-3348-5182"]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Vincent A. McKie","raw_affiliation_strings":["Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom and the","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059560909","display_name":"Harry John Gilbert","orcid":"https://orcid.org/0000-0003-3597-2347","observed_orcids":["https://orcid.org/0000-0003-3597-2347"]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Harry J. Gilbert","raw_affiliation_strings":["Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom and the","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004810388","display_name":"Richard W. Pickersgill","orcid":"https://orcid.org/0000-0001-6600-2381","observed_orcids":["https://orcid.org/0000-0001-6600-2381"]},"institutions":[{"id":"https://openalex.org/I166337079","display_name":"Queen Mary University of London","ror":"https://ror.org/026zzn846","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I166337079"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Richard W. Pickersgill","raw_affiliation_strings":["School of Biological Sciences, Medical Sciences Building, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Sciences, Medical Sciences Building, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom","institution_ids":["https://openalex.org/I166337079"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5004810388"],"corresponding_institution_ids":["https://openalex.org/I166337079"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":4.5811,"has_fulltext":true,"cited_by_count":93,"citation_normalized_percentile":{"value":0.94958216,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"276","issue":"33","first_page":"31186","last_page":"31192"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.8852999806404114,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.8852999806404114,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.03849999979138374,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.026499999687075615,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/substrate-binding","display_name":"substrate binding","score":0.6520000100135803},{"id":"https://openalex.org/keywords/catalytic-glutamate","display_name":"catalytic glutamate","score":0.9309999942779541},{"id":"https://openalex.org/keywords/manno-oligosaccharides","display_name":"manno-oligosaccharides","score":0.8349999785423279},{"id":"https://openalex.org/keywords/polysaccharide-degradation","display_name":"polysaccharide degradation","score":0.7860000133514404},{"id":"https://openalex.org/keywords/enzyme-active-site-residues","display_name":"enzyme active site residues","score":0.5320000052452087}],"concepts":[{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.6264712810516357},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5938376784324646},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.495232492685318},{"id":"https://openalex.org/C115624301","wikidata":"https://www.wikidata.org/wiki/Q895901","display_name":"Crystal structure","level":2,"score":0.44364750385284424},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43536531925201416},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.2797798812389374},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19063407182693481},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1558070182800293},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003461","descriptor_name":"Crystallography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003461","descriptor_name":"Crystallography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003461","descriptor_name":"Crystallography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008351","descriptor_name":"Mannans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008351","descriptor_name":"Mannans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008351","descriptor_name":"Mannans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008361","descriptor_name":"Mannosidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008361","descriptor_name":"Mannosidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008361","descriptor_name":"Mannosidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008361","descriptor_name":"Mannosidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008361","descriptor_name":"Mannosidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008361","descriptor_name":"Mannosidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044902","descriptor_name":"beta-Mannosidase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044902","descriptor_name":"beta-Mannosidase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044902","descriptor_name":"beta-Mannosidase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1074/jbc.m010290200","doi":"https://doi.org/10.1074/jbc.m010290200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m010290200","pdf_url":"http://www.jbc.org/article/S0021925820802725/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:11382747","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11382747","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"doi:10.25405/ncl.34600110","doi":"https://doi.org/10.25405/ncl.34600110","is_oa":true,"landing_page_url":"https://doi.org/10.25405/ncl.34600110","pdf_url":null,"source":{"id":"https://openalex.org/S4306402485","display_name":"Newcastle University ePrints (Newcastle Univesity)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I84884186","host_organization_name":"Newcastle University","host_organization_lineage":["https://openalex.org/I84884186"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"doi:10.25405/ncl.34600110.v1","doi":"https://doi.org/10.25405/ncl.34600110.v1","is_oa":true,"landing_page_url":"https://doi.org/10.25405/ncl.34600110.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306402485","display_name":"Newcastle University ePrints (Newcastle Univesity)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I84884186","host_organization_name":"Newcastle University","host_organization_lineage":["https://openalex.org/I84884186"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.1074/jbc.m010290200","doi":"https://doi.org/10.1074/jbc.m010290200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m010290200","pdf_url":"http://www.jbc.org/article/S0021925820802725/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation","score":0.4099999964237213}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332167","display_name":"Directorate for Biological Sciences","ror":"https://ror.org/001xhss06"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"},{"id":"https://openalex.org/F4320334759","display_name":"Deutsches Elektronen-Synchrotron","ror":"https://ror.org/01js2sh04"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1964107343.pdf","grobid_xml":"https://content.openalex.org/works/W1964107343.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W378873212","https://openalex.org/W1520026927","https://openalex.org/W1539796472","https://openalex.org/W1752301869","https://openalex.org/W1953098791","https://openalex.org/W1960470578","https://openalex.org/W1968637728","https://openalex.org/W1976800522","https://openalex.org/W1978230195","https://openalex.org/W1984297551","https://openalex.org/W1986191025","https://openalex.org/W1993108822","https://openalex.org/W2001641653","https://openalex.org/W2007404842","https://openalex.org/W2013083986","https://openalex.org/W2013900027","https://openalex.org/W2028231353","https://openalex.org/W2030798502","https://openalex.org/W2034453583","https://openalex.org/W2038840577","https://openalex.org/W2057575507","https://openalex.org/W2066674713","https://openalex.org/W2074369001","https://openalex.org/W2074475209","https://openalex.org/W2084575458","https://openalex.org/W2089395894","https://openalex.org/W2091075510","https://openalex.org/W2091418652","https://openalex.org/W2092987196","https://openalex.org/W2095237995","https://openalex.org/W2136531181","https://openalex.org/W2137500068","https://openalex.org/W2144423272","https://openalex.org/W2169821755","https://openalex.org/W2170264257","https://openalex.org/W2239384431"],"related_works":["https://openalex.org/W4387497383","https://openalex.org/W2948807893","https://openalex.org/W2778153218","https://openalex.org/W2748952813","https://openalex.org/W1531601525","https://openalex.org/W4391375266","https://openalex.org/W2078814861","https://openalex.org/W2527526854","https://openalex.org/W1976181487","https://openalex.org/W1986764834"],"abstract_inverted_index":{"The":[0,29,65,148],"crystal":[1,84],"structure":[2,85],"of":[3,24,41,73,78,86,95,125,161],"Pseudomonas":[4],"cellulosa":[5],"mannanase":[6,79,131,162],"26A":[7,132,163],"has":[8],"been":[9],"solved":[10],"by":[11,82],"multiple":[12],"isomorphous":[13],"replacement":[14],"and":[15,44,154],"refined":[16],"at":[17,38,114],"1.85":[18],"A":[19],"resolution":[20],"to":[21,98,122],"an":[22],"R-factor":[23],"0.182":[25],"(R-free":[26],"=":[27],"0.211).":[28],"enzyme":[30],"comprises":[31],"(beta/alpha)(8)-barrel":[32],"architecture":[33],"with":[34],"two":[35],"catalytic":[36,100],"glutamates":[37,54],"the":[39,48,52,83,87,93,99,115,123,126,136,140,144,159],"ends":[40],"beta-strands":[42],"4":[43],"7":[45],"in":[46,55,111,130,143],"precisely":[47],"same":[49,137],"location":[50],"as":[51,139],"corresponding":[53],"other":[56,145],"4/7-superfamily":[57],"glycoside":[58,63,68],"hydrolase":[59],"enzymes":[60],"(clan":[61],"GH-A":[62,146],"hydrolases).":[64],"family":[66],"26":[67],"hydrolases":[69],"are":[70,156,167],"therefore":[71],"members":[72],"clan":[74],"GH-A.":[75],"Functional":[76],"analyses":[77],"26A,":[80],"informed":[81],"enzyme,":[88],"provided":[89],"important":[90,121,157,169],"insights":[91],"into":[92],"role":[94,110],"residues":[96],"close":[97],"glutamates.":[101],"These":[102],"data":[103,149],"showed":[104],"that":[105,152],"Trp-360":[106],"played":[107],"a":[108],"critical":[109],"binding":[112],"substrate":[113],"-1":[116],"subsite,":[117],"whereas":[118],"Tyr-285":[119],"was":[120],"function":[124,138],"nucleophile":[127],"catalyst.":[128],"His-211":[129],"does":[133],"not":[134],"have":[135],"equivalent":[141],"asparagine":[142],"enzymes.":[147],"also":[150],"suggest":[151],"Trp-217":[153],"Trp-162":[155],"for":[158,170],"activity":[160,171],"against":[164,172],"mannooligosaccharides":[165],"but":[166],"less":[168],"polysaccharides.":[173]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":5}],"updated_date":"2026-10-19T09:35:57.686232","created_date":"2025-10-10T00:00:00"}