{"id":"https://openalex.org/W1964042467","doi":"https://doi.org/10.1042/ba20070178","title":"Selection and characterization of Affibody\u00ae ligands to the transcription factor c\u2010Jun","display_name":"Selection and characterization of Affibody\u00ae ligands to the transcription factor c\u2010Jun","publication_year":2008,"publication_date":"2008-05-08","ids":{"openalex":"https://openalex.org/W1964042467","doi":"https://doi.org/10.1042/ba20070178","mag":"1964042467","pmid":"https://pubmed.ncbi.nlm.nih.gov/18260830"},"language":"en","primary_location":{"id":"doi:10.1042/ba20070178","doi":"https://doi.org/10.1042/ba20070178","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba20070178","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052101351","display_name":"Emma K. Lundberg","orcid":"https://orcid.org/0000-0001-7034-0850","observed_orcids":["https://orcid.org/0000-0001-7034-0850"]},"institutions":[{"id":"https://openalex.org/I4210135511","display_name":"AlbaNova","ror":"https://ror.org/044kkfr75","country_code":"SE","type":"facility","lineage":["https://openalex.org/I4210135511"]},{"id":"https://openalex.org/I86987016","display_name":"KTH Royal Institute of Technology","ror":"https://ror.org/026vcq606","country_code":"SE","type":"education","lineage":["https://openalex.org/I86987016"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Emma Lundberg","raw_affiliation_strings":["School of Biotechnology, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, Stockholm, Sweden","School of Biotechnology, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, SE\u2010106 91, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biotechnology, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, Stockholm, Sweden","institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"]},{"raw_affiliation_string":"School of Biotechnology, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, SE\u2010106 91, Stockholm, Sweden","institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059443988","display_name":"Hjalmar Brismar","orcid":"https://orcid.org/0000-0003-0578-4003","observed_orcids":["https://orcid.org/0000-0003-0578-4003"]},"institutions":[{"id":"https://openalex.org/I4210135511","display_name":"AlbaNova","ror":"https://ror.org/044kkfr75","country_code":"SE","type":"facility","lineage":["https://openalex.org/I4210135511"]},{"id":"https://openalex.org/I86987016","display_name":"KTH Royal Institute of Technology","ror":"https://ror.org/026vcq606","country_code":"SE","type":"education","lineage":["https://openalex.org/I86987016"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Hjalmar Brismar","raw_affiliation_strings":["Department of Cell Physics, Albanova University Center, Kungliga Tekniska Ho\u0308gskolan, SE-106 91, Stockholm, Sweden","Department of Cell Physics, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, SE\u2010106 91, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Physics, Albanova University Center, Kungliga Tekniska Ho\u0308gskolan, SE-106 91, Stockholm, Sweden","institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"]},{"raw_affiliation_string":"Department of Cell Physics, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, SE\u2010106 91, Stockholm, Sweden","institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5074744219","display_name":"Torbj\u00f6rn Gr\u00e4slund","orcid":"https://orcid.org/0000-0002-5391-600X","observed_orcids":["https://orcid.org/0000-0002-5391-600X"]},"institutions":[{"id":"https://openalex.org/I4210135511","display_name":"AlbaNova","ror":"https://ror.org/044kkfr75","country_code":"SE","type":"facility","lineage":["https://openalex.org/I4210135511"]},{"id":"https://openalex.org/I86987016","display_name":"KTH Royal Institute of Technology","ror":"https://ror.org/026vcq606","country_code":"SE","type":"education","lineage":["https://openalex.org/I86987016"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Torbj\u00f6rn Gr\u00e4slund","raw_affiliation_strings":["School of Biotechnology, Albanova University Center, Kungliga Tekniska Ho\u0308gskolan, SE-106 91, Stockholm, Sweden","School of Biotechnology, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, SE\u2010106 91, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biotechnology, Albanova University Center, Kungliga Tekniska Ho\u0308gskolan, SE-106 91, Stockholm, Sweden","institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"]},{"raw_affiliation_string":"School of Biotechnology, Albanova University Center, Kungliga Tekniska H\u00f6gskolan, SE\u2010106 91, Stockholm, Sweden","institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5074744219"],"corresponding_institution_ids":["https://openalex.org/I4210135511","https://openalex.org/I86987016"],"apc_list":{"value":3470,"currency":"USD","value_usd":3470},"apc_paid":null,"fwci":2.5597,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.87726002,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"52","issue":"1","first_page":"17","last_page":"27"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/c-jun","display_name":"c-Jun","score":0.9869999885559082},{"id":"https://openalex.org/keywords/transcription-factors","display_name":"transcription factors","score":0.7829999923706055},{"id":"https://openalex.org/keywords/phage-display","display_name":"phage display","score":0.9070000052452087},{"id":"https://openalex.org/keywords/protein-binding-affinity","display_name":"protein binding affinity","score":0.4729999899864197},{"id":"https://openalex.org/keywords/combinatorial-protein-engineering","display_name":"combinatorial protein engineering","score":0.6520000100135803},{"id":"https://openalex.org/keywords/cooperative-binding","display_name":"cooperative binding","score":0.8019999861717224},{"id":"https://openalex.org/keywords/intracellular-protein-detection","display_name":"intracellular protein detection","score":0.4650000035762787},{"id":"https://openalex.org/keywords/oncoprotein","display_name":"oncoprotein","score":0.5899999737739563},{"id":"https://openalex.org/keywords/binding-epitope","display_name":"binding epitope","score":0.531000018119812}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5905543565750122},{"id":"https://openalex.org/C186268636","wikidata":"https://www.wikidata.org/wiki/Q896217","display_name":"Phage display","level":3,"score":0.5438980460166931},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5098909139633179},{"id":"https://openalex.org/C2776471321","wikidata":"https://www.wikidata.org/wiki/Q7166526","display_name":"Peptide library","level":4,"score":0.4846085011959076},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.4832075834274292},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4574718475341797},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.44476965069770813},{"id":"https://openalex.org/C74998103","wikidata":"https://www.wikidata.org/wiki/Q898254","display_name":"Dissociation constant","level":3,"score":0.43669265508651733},{"id":"https://openalex.org/C2779087070","wikidata":"https://www.wikidata.org/wiki/Q5005943","display_name":"c-jun","level":4,"score":0.43648597598075867},{"id":"https://openalex.org/C2780430158","wikidata":"https://www.wikidata.org/wiki/Q7685902","display_name":"Target protein","level":3,"score":0.41468772292137146},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40409308671951294},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3942975401878357},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3713986277580261},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.22693970799446106},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.21486768126487732},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16681215167045593},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.11942929029464722}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001667","descriptor_name":"Binding, Competitive","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016755","descriptor_name":"Proto-Oncogene Proteins c-jun","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016755","descriptor_name":"Proto-Oncogene Proteins c-jun","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019151","descriptor_name":"Peptide Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055767","descriptor_name":"Immobilized Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1042/ba20070178","doi":"https://doi.org/10.1042/ba20070178","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba20070178","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:18260830","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18260830","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W289505252","https://openalex.org/W1518343494","https://openalex.org/W1567848155","https://openalex.org/W1949973946","https://openalex.org/W1965706807","https://openalex.org/W1966886639","https://openalex.org/W1973974738","https://openalex.org/W1975548192","https://openalex.org/W1985206160","https://openalex.org/W1987897953","https://openalex.org/W1989588669","https://openalex.org/W1990850715","https://openalex.org/W1993870692","https://openalex.org/W1994219871","https://openalex.org/W2005537634","https://openalex.org/W2012536704","https://openalex.org/W2013535202","https://openalex.org/W2034375388","https://openalex.org/W2035758624","https://openalex.org/W2036224418","https://openalex.org/W2043048893","https://openalex.org/W2045586192","https://openalex.org/W2048408428","https://openalex.org/W2065335520","https://openalex.org/W2071957951","https://openalex.org/W2093519703","https://openalex.org/W2094330253","https://openalex.org/W2099330397","https://openalex.org/W2103719918","https://openalex.org/W2124597917","https://openalex.org/W2128130662","https://openalex.org/W2134450909","https://openalex.org/W2161579926","https://openalex.org/W2170915150"],"related_works":["https://openalex.org/W6996522143","https://openalex.org/W84945160","https://openalex.org/W2171223865","https://openalex.org/W7164388855","https://openalex.org/W1711405391","https://openalex.org/W2393680218","https://openalex.org/W2103719918","https://openalex.org/W7048158099","https://openalex.org/W604607026","https://openalex.org/W2045132978"],"abstract_inverted_index":{"c-Jun":[0,43,78,107,124,146,160,186,228,258],"is":[1,276],"a":[2,26,38,71,77,84,136,191,197,242,252,257],"highly":[3],"oncogenic":[4,274],"transcription":[5,270],"factor":[6],"involved":[7],"in":[8,48,96,154,170,190],"the":[9,17,55,58,91,155,158,165,185,208,224,230,237,247],"development":[10],"of":[11,14,29,37,57,131,140,164,196,240,265],"different":[12],"types":[13],"cancer.":[15],"In":[16,110],"present":[18,189],"study":[19],"we":[20],"have":[21],"generated":[22],"c-Jun-binding-affinity":[23],"proteins":[24],"from":[25,90,219,236],"phage-displayed":[27],"library":[28],"so-called":[30],"'Affibody":[31],"ligands',":[32],"developed":[33],"by":[34,63,100,214],"combinatorial":[35],"engineering":[36],"non-immunoglobulin-based":[39],"scaffold":[40],"protein.":[41,161],"Homodimeric":[42],"protein":[44,60,125,193],"was":[45,61,108,118,188,234],"recombinantly":[46],"produced":[47],"Escherichia":[49],"coli":[50],"and,":[51],"prior":[52],"to":[53,70,83,120,157,178,206],"selection,":[54],"quality":[56],"target":[59,187],"investigated":[62],"binding":[64,69,156,225,238],"analyses,":[65,112],"which":[66],"indicated":[67,222],"specific":[68],"double-stranded":[72,253],"DNA":[73,254],"hairpin":[74,255],"construct":[75],"containing":[76,256],"response":[79,259],"element,":[80],"but":[81],"not":[82],"control":[85],"sequence.":[86],"Isolated":[87],"Affibody":[88,114,167,232,266],"variants":[89],"phage":[92],"selection":[93],"were":[94],"expressed":[95],"E.":[97],"coli,":[98],"purified":[99],"affinity":[101,144],"chromatography":[102],"and":[103,173,251,272],"their":[104],"interaction":[105],"with":[106,122,126,181],"analysed.":[109],"biosensor":[111],"one":[113],"ligand,":[115],"denoted":[116],"Z(cJun518),":[117,141],"shown":[119],"interact":[121,179],"immobilized":[123,159],"an":[127],"apparent":[128,143],"dissociation":[129],"constant":[130],"5":[132],"microM.":[133],"By":[134],"constructing":[135],"head-to-tail":[137],"homodimeric":[138],"version":[139],"its":[142,176],"for":[145,229],"could":[147,202],"be":[148,204],"increased":[149],"threefold,":[150],"suggesting":[151],"co-operativity":[152],"effects":[153],"Further":[162],"characterization":[163],"Z(cJun518)":[166,201,231],"molecule":[168,233],"demonstrated,":[169],"both":[171,241],"affinity-capture":[172],"Western-blotting":[174],"experiments,":[175],"ability":[177],"selectively":[180],"c-Jun,":[182],"even":[183],"when":[184],"complex":[192],"background":[194],"consisting":[195],"bacterial":[198],"cell":[199,210],"lysate.":[200],"also":[203],"used":[205],"stain":[207],"c-Jun-overexpressing":[209],"line":[211],"C8161":[212],"visualized":[213],"confocal":[215],"fluorescence":[216],"microscopy.":[217],"Results":[218],"competition":[220],"experiments":[221],"that":[223],"epitope":[226],"on":[227],"separate":[235],"sites":[239],"polyclonal":[243],"antibody":[244],"raised":[245],"against":[246,269],"unstructured":[248],"N-terminal":[249],"domain":[250],"element.":[260],"The":[261],"potential":[262],"intracellular":[263],"use":[264],"ligands":[267],"directed":[268],"factors":[271,275],"other":[273],"discussed.":[277]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
