{"id":"https://openalex.org/W195634606","doi":"https://doi.org/10.2144/99274st08","title":"Budding Yeast as a Screening Tool for Discovery of Nucleoside Analogs for Use in HSV-1 TK Suicide-Gene Therapy","display_name":"Budding Yeast as a Screening Tool for Discovery of Nucleoside Analogs for Use in HSV-1 TK Suicide-Gene Therapy","publication_year":1999,"publication_date":"1999-10-01","ids":{"openalex":"https://openalex.org/W195634606","doi":"https://doi.org/10.2144/99274st08","mag":"195634606","pmid":"https://pubmed.ncbi.nlm.nih.gov/10524320"},"language":"en","primary_location":{"id":"doi:10.2144/99274st08","doi":"https://doi.org/10.2144/99274st08","is_oa":true,"landing_page_url":"https://doi.org/10.2144/99274st08","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/99274st08","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055706034","display_name":"Stefaan Wera","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"S. Wera","raw_affiliation_strings":["Katholieke Universiteit Leuven","Katholieke Universiteit , Leuven, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]},{"raw_affiliation_string":"Katholieke Universiteit , Leuven, Belgium","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025603977","display_name":"Bart Degr\u00e8ve","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24156275","display_name":"Rega Institute for Medical Research","ror":"https://ror.org/03w5j8p12","country_code":"BE","type":"facility","lineage":["https://openalex.org/I24156275"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"B. Degr\u00e8ve","raw_affiliation_strings":["Rega Institute for Medical Research Leuven, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rega Institute for Medical Research Leuven, Belgium","institution_ids":["https://openalex.org/I24156275"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038319371","display_name":"Jan M. Balzarini","orcid":"https://orcid.org/0000-0003-2240-3321","observed_orcids":["https://orcid.org/0000-0003-2240-3321","https://orcid.org/0000-0003-1144-1808"]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"J. Balzarini","raw_affiliation_strings":["Katholieke Universiteit Leuven","Katholieke Universiteit , Leuven, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]},{"raw_affiliation_string":"Katholieke Universiteit , Leuven, Belgium","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040662398","display_name":"Erik De Clercq","orcid":"https://orcid.org/0000-0002-2985-8890","observed_orcids":["https://orcid.org/0000-0002-2985-8890"]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"E. De Clercq","raw_affiliation_strings":["Katholieke Universiteit Leuven","Katholieke Universiteit , Leuven, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]},{"raw_affiliation_string":"Katholieke Universiteit , Leuven, Belgium","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035778660","display_name":"Johan M. Thevelein","orcid":"https://orcid.org/0000-0002-2626-0180","observed_orcids":["https://orcid.org/0000-0002-2626-0180"]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"J.M. Thevelein","raw_affiliation_strings":["Katholieke Universiteit Leuven","Katholieke Universiteit , Leuven, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]},{"raw_affiliation_string":"Katholieke Universiteit , Leuven, Belgium","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089010495","display_name":"Johan H. Neyts","orcid":"https://orcid.org/0000-0002-0033-7514","observed_orcids":["https://orcid.org/0000-0002-0033-7514"]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"J. Neyts","raw_affiliation_strings":["Katholieke Universiteit Leuven","Katholieke Universiteit , Leuven, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]},{"raw_affiliation_string":"Katholieke Universiteit , Leuven, Belgium","institution_ids":["https://openalex.org/I99464096"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.02570903,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"27","issue":"4","first_page":"772","last_page":"777"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.47189998626708984,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.47189998626708984,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.2290000021457672,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.13830000162124634,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hsv-1-thymidine-kinase","display_name":"HSV-1 thymidine kinase","score":0.9610000252723694},{"id":"https://openalex.org/keywords/nucleoside-analogs","display_name":"nucleoside analogues","score":0.925000011920929},{"id":"https://openalex.org/keywords/suicide-gene-therapy","display_name":"suicide gene therapy","score":0.9430000185966492},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8899999856948853},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7099999785423279},{"id":"https://openalex.org/keywords/chemical-library-screening","display_name":"chemical library screening","score":0.6990000009536743},{"id":"https://openalex.org/keywords/acyclovir","display_name":"acyclovir","score":0.7329999804496765},{"id":"https://openalex.org/keywords/ganciclovir","display_name":"ganciclovir","score":0.9959999918937683},{"id":"https://openalex.org/keywords/penciclovir","display_name":"penciclovir","score":0.996999979019165},{"id":"https://openalex.org/keywords/brivudin","display_name":"brivudin","score":0.9419999718666077},{"id":"https://openalex.org/keywords/sorivudine","display_name":"sorivudine","score":0.8980000019073486},{"id":"https://openalex.org/keywords/cytostatic-effect","display_name":"cytostatic activity","score":0.640999972820282},{"id":"https://openalex.org/keywords/multicopy-plasmid","display_name":"multicopy plasmid","score":0.6539999842643738}],"concepts":[{"id":"https://openalex.org/C2776147627","wikidata":"https://www.wikidata.org/wiki/Q24783855","display_name":"Thymidine kinase","level":4,"score":0.8167818784713745},{"id":"https://openalex.org/C34981463","wikidata":"https://www.wikidata.org/wiki/Q3311663","display_name":"Suicide gene","level":4,"score":0.7655096650123596},{"id":"https://openalex.org/C2780796145","wikidata":"https://www.wikidata.org/wiki/Q420364","display_name":"Penciclovir","level":4,"score":0.7130551338195801},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7028366327285767},{"id":"https://openalex.org/C2777965375","wikidata":"https://www.wikidata.org/wiki/Q417640","display_name":"Ganciclovir","level":4,"score":0.639981746673584},{"id":"https://openalex.org/C2781196997","wikidata":"https://www.wikidata.org/wiki/Q655331","display_name":"Herpes simplex virus","level":3,"score":0.5601652264595032},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5131528973579407},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.4975173771381378},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4947492480278015},{"id":"https://openalex.org/C2776543447","wikidata":"https://www.wikidata.org/wiki/Q28734","display_name":"Nucleoside","level":2,"score":0.4664463698863983},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.43913936614990234},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4368714392185211},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.40216144919395447},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.3525286912918091},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.29173383116722107},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2670828402042389},{"id":"https://openalex.org/C2779820661","wikidata":"https://www.wikidata.org/wiki/Q6929","display_name":"Human cytomegalovirus","level":3,"score":0.2510978579521179},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.06334555149078369},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000212","descriptor_name":"Acyclovir","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000998","descriptor_name":"Antiviral Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D013937","descriptor_name":"Thymidine Kinase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018259","descriptor_name":"Herpesvirus 1, Human","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.2144/99274st08","doi":"https://doi.org/10.2144/99274st08","is_oa":true,"landing_page_url":"https://doi.org/10.2144/99274st08","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:10524320","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10524320","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Research Support, Non-U.S. Gov't"},{"id":"pmh:oai:doaj.org/article:362dba905586471682c58b7cfb238123","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/362dba905586471682c58b7cfb238123","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 27, Iss 4, Pp 772-777 (1999)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/99274st08","doi":"https://doi.org/10.2144/99274st08","is_oa":true,"landing_page_url":"https://doi.org/10.2144/99274st08","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2012636731","https://openalex.org/W1992322591","https://openalex.org/W2329169879","https://openalex.org/W7099506188","https://openalex.org/W1889433567","https://openalex.org/W2951030440","https://openalex.org/W2141070882","https://openalex.org/W7099009358","https://openalex.org/W2439321259","https://openalex.org/W2467361245"],"abstract_inverted_index":{"We":[0],"present":[1],"a":[2,57,126,167],"fast,":[3],"convenient":[4],"and":[5,70],"inexpensive":[6],"method":[7,39,161],"that":[8,19],"allows":[9,142],"the":[10,23,43,52,74,81,96,101,114,120,129,134,174],"automated,":[11],"large-scale":[12],"screening":[13,144],"of":[14,35,45,98,116,131,138,176,179,182],"chemical":[15],"libraries":[16],"for":[17,72,91,149],"compounds":[18,150],"are":[20],"converted":[21],"by":[22],"herpes":[24],"simplex":[25],"virus":[26],"type":[27],"1":[28],"(HSV-1)":[29],"thymidine":[30],"kinase":[31],"(TK)":[32],"into":[33],"inhibitors":[34],"cell":[36,185],"growth.":[37],"The":[38,160],"is":[40],"based":[41],"on":[42,56,95,113],"use":[44,153],"budding":[46],"yeast":[47,99,132,141,180,184],"(Saccharomyces":[48],"cerevisiae)":[49],"transformed":[50,118],"with":[51,119,151],"HSV-1":[53,82,139,155],"TK":[54,83,156],"gene":[55,84,158],"multicopy":[58],"plasmid.":[59],"Eight":[60],"nucleoside":[61,105],"analogs":[62,106],"(acyclovir,":[63],"ganciclovir,":[64],"penciclovir,":[65],"lobucavir,":[66],"brivudin,":[67],"sorivudine,":[68],"IVDU":[69],"ara-T),":[71],"which":[73],"cytostatic":[75],"action":[76],"against":[77],"mammalian":[78],"cells":[79],"expressing":[80,100,133],"has":[85],"been":[86],"well":[87],"documented,":[88],"were":[89],"studied":[90],"their":[92],"inhibitory":[93,111],"effect":[94,112],"growth":[97,115,130,175],"viral":[102,135],"TK.":[103,136],"These":[104],"had":[107],"little":[108],"or":[109,172],"no":[110],"yeasts":[117],"empty":[121],"vector,":[122],"but":[123],"inhibited":[124],"to":[125,169],"significant":[127],"extent":[128],"Use":[137],"TK-expressing":[140],"quick":[143],"in":[145,154],"multi-well":[146],"plate":[147],"format":[148],"potential":[152],"suicide":[157],"therapy.":[159],"may":[162],"also":[163],"be":[164],"used":[165],"as":[166],"tool":[168],"selectively":[170],"suppress":[171],"arrest":[173],"one":[177],"population":[178],"out":[181],"mixed":[183],"cultures.":[186]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}