{"id":"https://openalex.org/W1948708264","doi":"https://doi.org/10.1093/genetics/149.2.857","title":"Modulation of Tubulin Polypeptide Ratios by the Yeast Protein Pac10p","display_name":"Modulation of Tubulin Polypeptide Ratios by the Yeast Protein Pac10p","publication_year":1998,"publication_date":"1998-06-01","ids":{"openalex":"https://openalex.org/W1948708264","doi":"https://doi.org/10.1093/genetics/149.2.857","mag":"1948708264","pmid":"https://pubmed.ncbi.nlm.nih.gov/9611197","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1460170"},"language":"en","primary_location":{"id":"doi:10.1093/genetics/149.2.857","doi":"https://doi.org/10.1093/genetics/149.2.857","is_oa":false,"landing_page_url":"https://doi.org/10.1093/genetics/149.2.857","pdf_url":null,"source":{"id":"https://openalex.org/S65932378","display_name":"Genetics","issn_l":"0016-6731","issn":["0016-6731","1943-2631"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Pablo Alvarez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pablo Alvarez","raw_affiliation_strings":["Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111511747","display_name":"Adelle Smith","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adelle Smith","raw_affiliation_strings":["Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081001764","display_name":"James Fleming","orcid":"https://orcid.org/0000-0002-9259-3522","observed_orcids":["https://orcid.org/0000-0002-9259-3522"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James Fleming","raw_affiliation_strings":["Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108451279","display_name":"Frank Solomon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Frank Solomon","raw_affiliation_strings":["Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5108451279"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":4477,"currency":"USD","value_usd":4477},"apc_paid":null,"fwci":0.9017,"has_fulltext":true,"cited_by_count":23,"citation_normalized_percentile":{"value":0.71338112,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"149","issue":"2","first_page":"857","last_page":"864"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11412","display_name":"Genetic and Kidney Cyst Diseases","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-tubulin","display_name":"alpha-tubulin","score":0.8510000109672546},{"id":"https://openalex.org/keywords/beta-tubulin","display_name":"beta-tubulin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/microtubule-polymerization","display_name":"microtubule assembly","score":0.8339999914169312},{"id":"https://openalex.org/keywords/microtubule-disassembly","display_name":"microtubule disassembly","score":0.9380000233650208},{"id":"https://openalex.org/keywords/yeast","display_name":"yeast","score":0.44999998807907104},{"id":"https://openalex.org/keywords/protein-levels","display_name":"protein abundance","score":0.31700000166893005}],"concepts":[{"id":"https://openalex.org/C84425145","wikidata":"https://www.wikidata.org/wiki/Q422492","display_name":"Tubulin","level":3,"score":0.9664453268051147},{"id":"https://openalex.org/C20418707","wikidata":"https://www.wikidata.org/wiki/Q189933","display_name":"Microtubule","level":2,"score":0.8548930287361145},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8325890898704529},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.562030017375946},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5264697074890137},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.4763469994068146},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.459725558757782},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.33525073528289795},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3178229331970215}],"mesh":[{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004722","descriptor_name":"Endoribonucleases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D005800","descriptor_name":"Genes, Fungal","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D005804","descriptor_name":"Genes, Lethal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008869","descriptor_name":"Microtubule-Associated Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016547","descriptor_name":"Kinesins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1093/genetics/149.2.857","doi":"https://doi.org/10.1093/genetics/149.2.857","is_oa":false,"landing_page_url":"https://doi.org/10.1093/genetics/149.2.857","pdf_url":null,"source":{"id":"https://openalex.org/S65932378","display_name":"Genetics","issn_l":"0016-6731","issn":["0016-6731","1943-2631"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetics","raw_type":"journal-article"},{"id":"pmid:9611197","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9611197","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetics","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.333.4373","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.333.4373","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.genetics.org/content/149/2/857.full.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W1597800304","https://openalex.org/W1608303629","https://openalex.org/W1802253821","https://openalex.org/W1816239584","https://openalex.org/W1856170453","https://openalex.org/W1879738300","https://openalex.org/W1894461767","https://openalex.org/W1897196422","https://openalex.org/W1920405460","https://openalex.org/W1965121426","https://openalex.org/W1969213324","https://openalex.org/W1973622904","https://openalex.org/W1985108909","https://openalex.org/W2018203229","https://openalex.org/W2027580481","https://openalex.org/W2037094686","https://openalex.org/W2037830911","https://openalex.org/W2046006262","https://openalex.org/W2052166374","https://openalex.org/W2075993250","https://openalex.org/W2096261825","https://openalex.org/W2100634607","https://openalex.org/W2133433041","https://openalex.org/W2142181044","https://openalex.org/W2159693676","https://openalex.org/W2167826437","https://openalex.org/W2350650972","https://openalex.org/W6681174304"],"related_works":["https://openalex.org/W4233917708","https://openalex.org/W4318670743","https://openalex.org/W2105186159","https://openalex.org/W1969213324","https://openalex.org/W7134975423","https://openalex.org/W2037144229","https://openalex.org/W1597800304","https://openalex.org/W4213220166","https://openalex.org/W2115568470","https://openalex.org/W1879738300"],"abstract_inverted_index":{"Normal":[0],"assembly":[1],"and":[2,33,59,162,167],"function":[3],"of":[4,8,12,27,41,50,70,79,98,109,114,145,157],"microtubules":[5],"require":[6],"maintenance":[7],"the":[9,16,38,51,68,71,76,80,88,112,120,143,155,160],"proper":[10],"levels":[11,69,78,97],"several":[13],"proteins,":[14],"including":[15],"tubulin":[17,81,168],"polypeptides":[18],"themselves.":[19],"For":[20],"example,":[21],"in":[22,142,159],"yeast":[23,72],"a":[24,131,139],"significant":[25],"excess":[26,40,124],"beta-tubulin":[28,42,132],"causes":[29],"rapid":[30],"microtubule":[31,62,165],"disassembly":[32],"subsequent":[34],"cell":[35,57],"death.":[36],"Even":[37],"modest":[39],"produced":[43],"by":[44],"genetic":[45],"alterations":[46],"such":[47],"as":[48,91],"deletion":[49],"minor":[52],"alpha-tubulin":[53,99,115,146,158],"gene":[54],"TUB3":[55],"affects":[56],"growth":[58],"can":[60],"confer":[61],"phenotypes.":[63],"We":[64],"show":[65,138],"here":[66],"that":[67,94,104,152],"protein":[73],"Pac10p":[74,110,127,153],"affect":[75],"relative":[77],"polypeptides.":[82],"Cells":[83],"deleted":[84],"for":[85],"PAC10":[86],"have":[87],"same":[89],"phenotypes":[90],"do":[92],"cells":[93,137],"express":[95],"reduced":[96],"or":[100,116],"Rbl2p,":[101],"two":[102],"proteins":[103],"bind":[105],"beta-tubulin.":[106,125,148],"Conversely,":[107],"overexpression":[108],"enhances":[111],"ability":[113],"Rbl2p":[117],"to":[118,147],"suppress":[119],"lethality":[121],"associated":[122],"with":[123],"However,":[126],"is":[128],"itself":[129],"not":[130],"binding":[133],"protein.":[134],"Pac10":[135],"null":[136],"30%":[140],"decrease":[141],"ratio":[144],"The":[149],"results":[150],"suggest":[151],"modulates":[154],"level":[156],"cell,":[161],"so":[163],"influences":[164],"morphogenesis":[166],"metabolism.":[169]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}