{"id":"https://openalex.org/W1936601620","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb06446.x","title":"Reconstitution of Lactic Dehydrogenase after Acid Dissociation","display_name":"Reconstitution of Lactic Dehydrogenase after Acid Dissociation","publication_year":1981,"publication_date":"1981-12-01","ids":{"openalex":"https://openalex.org/W1936601620","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb06446.x","mag":"1936601620","pmid":"https://pubmed.ncbi.nlm.nih.gov/7327168"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1981.tb06446.x","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb06446.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1981.tb06446.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088878291","display_name":"Gerd Zettlmei\u00dfl","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Gerd ZETTLMEISSL","raw_affiliation_strings":["Biochemie II, Naturwissenschaftliche Fakulta\u0308t III der Universita\u0308t Regensburg, Universita\u0308tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","Biochemie II, Naturwissenschaftliche Fakult\u00e4t III der Universit\u00e4t Regensburg, Universit\u00e4tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemie II, Naturwissenschaftliche Fakulta\u0308t III der Universita\u0308t Regensburg, Universita\u0308tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","institution_ids":["https://openalex.org/I60668342"]},{"raw_affiliation_string":"Biochemie II, Naturwissenschaftliche Fakult\u00e4t III der Universit\u00e4t Regensburg, Universit\u00e4tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","institution_ids":["https://openalex.org/I60668342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110603064","display_name":"Rainer Rudolph","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Rainer RUDOLPH","raw_affiliation_strings":["Biochemie II, Naturwissenschaftliche Fakulta\u0308t III der Universita\u0308t Regensburg, Universita\u0308tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","Biochemie II, Naturwissenschaftliche Fakult\u00e4t III der Universit\u00e4t Regensburg, Universit\u00e4tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemie II, Naturwissenschaftliche Fakulta\u0308t III der Universita\u0308t Regensburg, Universita\u0308tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","institution_ids":["https://openalex.org/I60668342"]},{"raw_affiliation_string":"Biochemie II, Naturwissenschaftliche Fakult\u00e4t III der Universit\u00e4t Regensburg, Universit\u00e4tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","institution_ids":["https://openalex.org/I60668342"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023088787","display_name":"Rainer Jaenicke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60668342","display_name":"University of Regensburg","ror":"https://ror.org/01eezs655","country_code":"DE","type":"education","lineage":["https://openalex.org/I60668342"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Rainer JAENICKE","raw_affiliation_strings":["Biochemie II, Naturwissenschaftliche Fakulta\u0308t III der Universita\u0308t Regensburg, Universita\u0308tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","Biochemie II, Naturwissenschaftliche Fakult\u00e4t III der Universit\u00e4t Regensburg, Universit\u00e4tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemie II, Naturwissenschaftliche Fakulta\u0308t III der Universita\u0308t Regensburg, Universita\u0308tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","institution_ids":["https://openalex.org/I60668342"]},{"raw_affiliation_string":"Biochemie II, Naturwissenschaftliche Fakult\u00e4t III der Universit\u00e4t Regensburg, Universit\u00e4tsstra\u00dfe 31, D-8400 Regensburg, Federal Republic of Germany","institution_ids":["https://openalex.org/I60668342"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I60668342"],"apc_list":null,"apc_paid":null,"fwci":1.1848,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":{"value":0.74740403,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"121","issue":"1","first_page":"169","last_page":"175"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactate-dehydrogenase","display_name":"lactate dehydrogenase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/enzyme-reconstitution","display_name":"enzyme reconstitution","score":0.7979999780654907},{"id":"https://openalex.org/keywords/acid-dissociation","display_name":"acid dissociation","score":0.9070000052452087},{"id":"https://openalex.org/keywords/protein-denaturation","display_name":"protein denaturation","score":0.6740000247955322},{"id":"https://openalex.org/keywords/partial-unfolding","display_name":"partial unfolding","score":0.5630000233650208},{"id":"https://openalex.org/keywords/reactivation-kinetics","display_name":"reactivation kinetics","score":0.5199999809265137},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.8730000257492065},{"id":"https://openalex.org/keywords/far-ultraviolet-circular-dichroism","display_name":"far-ultraviolet circular dichroism","score":0.8610000014305115},{"id":"https://openalex.org/keywords/helix-content","display_name":"helix content","score":0.8389999866485596},{"id":"https://openalex.org/keywords/activation-energy","display_name":"activation energy","score":0.8569999933242798},{"id":"https://openalex.org/keywords/guanidine-hydrochloride","display_name":"guanidine hydrochloride","score":0.7950000166893005},{"id":"https://openalex.org/keywords/pig-skeletal-muscle","display_name":"pig skeletal muscle","score":0.5879999995231628}],"concepts":[{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.8488348126411438},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7854970693588257},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.6826778650283813},{"id":"https://openalex.org/C102931765","wikidata":"https://www.wikidata.org/wiki/Q189673","display_name":"Dissociation (chemistry)","level":2,"score":0.6712582111358643},{"id":"https://openalex.org/C2776923230","wikidata":"https://www.wikidata.org/wiki/Q16955798","display_name":"Denaturation (fissile materials)","level":2,"score":0.5199221968650818},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.468943327665329},{"id":"https://openalex.org/C2779463914","wikidata":"https://www.wikidata.org/wiki/Q183309","display_name":"Guanidine","level":2,"score":0.4431200623512268},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4308006167411804},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.41022202372550964},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3620235323905945},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2836509644985199},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.18639019131660461},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12168416380882263},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.07396882772445679},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.07277840375900269},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006146","descriptor_name":"Guanidines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019791","descriptor_name":"Guanidine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1981.tb06446.x","doi":"https://doi.org/10.1111/j.1432-1033.1981.tb06446.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1981.tb06446.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:7327168","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7327168","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W69390289","https://openalex.org/W268238884","https://openalex.org/W1994732788","https://openalex.org/W1997088693","https://openalex.org/W1998058974","https://openalex.org/W2006975595","https://openalex.org/W2012664948","https://openalex.org/W2014170948","https://openalex.org/W2014550271","https://openalex.org/W2017639307","https://openalex.org/W2028994061","https://openalex.org/W2053088135","https://openalex.org/W2054479934","https://openalex.org/W2122436159","https://openalex.org/W2253254707","https://openalex.org/W2626547789","https://openalex.org/W2975826617","https://openalex.org/W3026651526"],"related_works":["https://openalex.org/W2025021649","https://openalex.org/W2054479934","https://openalex.org/W1994732788","https://openalex.org/W1997088693","https://openalex.org/W2053088135","https://openalex.org/W2092389531","https://openalex.org/W2079312558","https://openalex.org/W2092383329","https://openalex.org/W2016385753","https://openalex.org/W2049986828"],"abstract_inverted_index":{"Reconstitution":[0],"of":[1,19,34,39,49,72,83,95,100,119,124,148,166,173,193,218,265,278,281,297,303,312,315,344,349],"lactic":[2,304],"dehydrogenase":[3,305],"from":[4,134,262],"pig":[5],"muscle":[6],"after":[7,138,146,225],"dissociation":[8],"at":[9,87,335],"acidic":[10],"pH":[11,89,231,237,299,337],"produces":[12],"native":[13],"tetramers":[14],"and":[15,31,51,57,98,157,191,211,239,283,347],"inactive":[16],"aggregates,":[17],"both":[18,273],"which":[20],"have":[21],"been":[22],"previously":[23],"described":[24],"with":[25,69,208],"respect":[26],"to":[27,91,141,144,164,170,205,245,290],"their":[28,35],"structural":[29,74,162,333],"properties":[30,159],"the":[32,40,46,70,77,81,96,107,120,125,139,149,171,174,179,185,189,198,201,219,222,248,253,257,263,266,269,274,279,284,301,313,316,331,341,345],"kinetics":[33],"formation.":[36],"The":[37,64,152,214,295],"ratio":[38],"two":[41],"fractions":[42],"is":[43,67,288],"determined":[44],"by":[45,103,116,309,320],"relative":[47],"rates":[48],"reassociation":[50],"aggregation":[52],"[G.":[53],"Zettlmei\u00dfI,":[54],"R.":[55,58],"Rudolph":[56],"Jaenicke":[59],"(1979)":[60],"Biochemistry":[61],"18,":[62],"5567\u20135571].":[63],"present":[65],"study":[66],"concerned":[68],"influence":[71],"residual":[73,175],"elements":[75],"within":[76,106],"acid\u2010denatured":[78],"monomers":[79],"on":[80,300],"process":[82],"reconstitution.":[84],"Long":[85],"incubation":[86,209,226],"low":[88,142,298],"leads":[90],"a":[92,129],"marked":[93],"decrease":[94,130,187,220,343],"rate":[97,190,216,267,346],"yield":[99,192,224,280,348],"reactivation,":[101,194],"caused":[102],"conformational":[104],"changes":[105,118,172,199,255,277],"partially":[108],"unfolded":[109],"monomers.":[110],"These":[111],"slow":[112,186],"rearrangements":[113,334],"are":[114,338],"reflected":[115],"slight":[117],"far\u2010ultraviolet":[121,202,258,285],"circular":[122,155,203,259,286],"dichroism":[123,156,204,287],"acid\u2010dissociated":[126],"monomers,":[127],"indicating":[128],"in":[131,188,200,221,227,247,256],"helix":[132],"content":[133],"30":[135],"observed":[136,342],"immediately":[137],"transfer":[140],"pH,":[143],"25%":[145],"completion":[147],"acid":[150,275,336],"transition.":[151],"unchanged":[153],"near\u2010ultraviolet":[154],"fluorescence":[158],"prove":[160],"local":[161],"alterations":[163],"be":[165,206,246,291,307],"lesser":[167],"importance":[168],"compared":[169],"backbone":[176],"folding.":[177],"Varying":[178],"experimental":[180],"conditions":[181],"during":[182],"denaturation":[183],"proves":[184,328],"as":[195,197,252],"well":[196],"enhanced":[207],"time":[210,250],"temperature":[212],"(0\u201330\u00b0C).":[213],"first\u2010order":[215],"constants":[217],"reactivation":[223,282],"1":[228,240],"M":[229,234,241,323],"glycine/H3PO4":[230],"2.3,":[232,238],"0.1":[233],"sodium":[235],"phosphate":[236],"H3PO4":[242],"were":[243],"found":[244,289],"same":[249],"range":[251],"respective":[254],"dichroism.":[260],"Apart":[261],"similarity":[264],"constants,":[268],"activation":[270],"energy":[271],"for":[272,340],"induced":[276],"identical":[292],"(80":[293],"kJ/mol).":[294],"effects":[296],"reconstitution":[302],"can":[306],"eliminated":[308],"complete":[310],"disruption":[311],"structure":[314],"polypeptide":[317],"chain,":[318],"e.g.":[319],"adding":[321],"6":[322],"guanidine":[324],"\u00b7":[325],"HCl.":[326],"This":[327],"unambiguously":[329],"that":[330],"above\u2010mentioned":[332],"responsible":[339],"reactivation.":[350]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2017,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
