{"id":"https://openalex.org/W192706554","doi":"https://doi.org/10.31274/etd-180810-3618","title":"Characterization of Ebola virus VP35 first basic patch as a therapeutic target","display_name":"Characterization of Ebola virus VP35 first basic patch as a therapeutic target","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W192706554","doi":"https://doi.org/10.31274/etd-180810-3618","mag":"192706554"},"language":"en","primary_location":{"id":"doi:10.31274/etd-180810-3618","doi":"https://doi.org/10.31274/etd-180810-3618","is_oa":false,"landing_page_url":"https://doi.org/10.31274/etd-180810-3618","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iowa State University, Digital Repository","raw_type":"dissertation"},"volume":null,"type":"dissertation","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003937372","display_name":"Jennifer M. Binning","orcid":"https://orcid.org/0000-0001-5871-686X","observed_orcids":["https://orcid.org/0000-0001-5871-686X"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jennifer Binning","raw_affiliation_strings":["Iowa State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Iowa State University","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5003937372"],"corresponding_institution_ids":["https://openalex.org/I173911158"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11581","display_name":"Viral Infections and Outbreaks Research","score":0.9672999978065491,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11581","display_name":"Viral Infections and Outbreaks Research","score":0.9672999978065491,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.016599999740719795,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11668","display_name":"interferon and immune responses","score":0.006000000052154064,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ebola-virus","display_name":"Ebola virus","score":0.8399999737739563},{"id":"https://openalex.org/keywords/vp35","display_name":"VP35","score":0.9570000171661377},{"id":"https://openalex.org/keywords/dsrna-binding","display_name":"dsRNA binding","score":0.8119999766349792},{"id":"https://openalex.org/keywords/interferon-antagonism","display_name":"interferon antagonism","score":0.6890000104904175},{"id":"https://openalex.org/keywords/aptamers","display_name":"aptamers","score":0.6840000152587891},{"id":"https://openalex.org/keywords/selex","display_name":"SELEX","score":0.9649999737739563},{"id":"https://openalex.org/keywords/antiviral-drug-targets","display_name":"antiviral drug targets","score":0.7699999809265137},{"id":"https://openalex.org/keywords/filoviruses","display_name":"filoviruses","score":0.6019999980926514},{"id":"https://openalex.org/keywords/rna-dependent-rna-polymerase","display_name":"RNA-dependent RNA polymerase","score":0.5669999718666077},{"id":"https://openalex.org/keywords/viral-replication-inhibition","display_name":"viral replication inhibition","score":0.4350000023841858},{"id":"https://openalex.org/keywords/protein-protein-interaction-inhibition","display_name":"protein-protein interaction inhibition","score":0.48399999737739563}],"concepts":[{"id":"https://openalex.org/C2777469322","wikidata":"https://www.wikidata.org/wiki/Q8064876","display_name":"Ebola virus","level":3,"score":0.7761917114257812},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5757759809494019},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.4704683721065521},{"id":"https://openalex.org/C2909623084","wikidata":"https://www.wikidata.org/wiki/Q5331908","display_name":"Ebolavirus","level":4,"score":0.426453173160553},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3674207925796509},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.28812235593795776},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.13827916979789734},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.07980716228485107}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.31274/etd-180810-3618","doi":"https://doi.org/10.31274/etd-180810-3618","is_oa":false,"landing_page_url":"https://doi.org/10.31274/etd-180810-3618","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iowa State University, Digital Repository","raw_type":"dissertation"},{"id":"pmh:oai:dr.lib.iastate.edu:20.500.12876/28073","doi":null,"is_oa":false,"landing_page_url":"https://dr.lib.iastate.edu/handle/20.500.12876/28073","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"archive/lib.dr.iastate.edu/etd/13886/Binning_iastate_0097E_14282.pdf|||Fri Jan 14 20:03:07 UTC 2022","raw_type":"dissertation"},{"id":"pmh:oai:lib.dr.iastate.edu:etd-4893","doi":null,"is_oa":false,"landing_page_url":"https://lib.dr.iastate.edu/etd/13886","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2118986667","https://openalex.org/W1984936484","https://openalex.org/W2162395676","https://openalex.org/W4412791598","https://openalex.org/W2017700325","https://openalex.org/W2137587180","https://openalex.org/W3217233727","https://openalex.org/W2155409207","https://openalex.org/W2010072146","https://openalex.org/W2584575235"],"abstract_inverted_index":{"Viral":[0],"protein":[1],"35":[2],"(VP35),":[3],"encoded":[4],"by":[5,351],"filoviruses,":[6],"are":[7,57,193,200],"multifunctional":[8,24,51],"dsRNA\\nbinding":[9],"proteins":[10,28,100,391,406],"that":[11,35,76,136,188,235,299,374,387],"play":[12],"important":[13,140,201],"roles":[14],"in":[15,88,146,215,366],"viral":[16,232,355],"replication,":[17],"innate":[18],"immune":[19],"evasion":[20],"and":[21,43,113,174,177,257,263,314,326,380,395,397],"pathogenesis.":[22],"The":[23,154],"nature":[25],"of":[26,46,64,82,156,166,172,211,255,306,345,353,370,384],"these":[27,212,252],"also":[29],"presents":[30],"opportunities":[31],"to":[32,71,79,134,151,161,228,249,293,303,324,335,401],"develop":[33],"countermeasures":[34],"target":[36,72,251,403],"distinct":[37,195,283,304],"functional":[38,41,164,253],"regions.":[39],"However,":[40],"validation":[42],"the":[44,61,80,108,114,121,137,163,167,191,197,208,220,231,236,244,300,328,333,343,346,371,376,382,399],"establishment":[45],"therapeutic":[47,68,183,409],"approaches":[48],"toward":[49],"such":[50,83],"proteins,":[52],"particularly":[53],"for":[54,141,202,274,322],"non-enzymatic":[55],"targets,":[56],"often":[58],"challenging.":[59],"With":[60],"current":[62],"lack":[63],"approved":[65],"vaccines":[66],"or":[67],"options":[69],"available":[70],"filoviral":[73,98,389,404],"infections,":[74],"work":[75,96,131,159],"lends":[77],"itself":[78],"development":[81],"inhibitors":[84],"will":[85],"be":[86],"instrumental":[87],"countering":[89],"this":[90],"highly":[91,103],"pathogenic":[92],"virus.":[93],"Our":[94],"previous":[95],"on":[97,133,289],"VP35":[99,142,179,203,213,237,256,259,272,390,405],"defined":[101],"two":[102,282,385],"conserved":[104,105],"basic":[106,110,116],"patches,":[107],"first":[109],"patch":[111,117],"(FBP)":[112],"central":[115],"(CBP),":[118],"located":[119],"within":[120,190],"C-terminal":[122],"dsRNA":[123,321],"binding":[124,323],"interferon":[125],"(IFN)":[126],"inhibitory":[127],"domain":[128],"(IID).":[129],"This":[130],"went":[132],"show":[135],"CBP":[138],"is":[139,217],"mediated":[143],"IFN":[144],"antagonism,":[145],"part":[147],"through":[148,169],"its":[149],"ability":[150,227,334],"bind":[152,302,388],"dsRNA.":[153],"goal":[155],"my":[157],"thesis":[158],"was":[160],"investigate":[162],"importance":[165],"FBP":[168,192,223,238],"a":[170,181,261,408],"combination":[171],"structural":[173],"biochemical":[175],"studies,":[176],"validate":[178,258],"as":[180,260,362,407],"potential":[182,262],"target.":[184,410],"These":[185,296,316],"efforts":[186],"established":[187],"residues":[189,214],"functionally":[194],"from":[196],"CBP,":[198],"but":[199],"polymerase":[204,348,378],"co-factor":[205],"function.":[206],"Although":[207],"exact":[209],"role":[210],"replication":[216,221],"poorly":[218],"defined,":[219],"defective":[222],"mutants":[224],"lost":[225],"their":[226,290],"interact":[229],"with":[230,309,320,332,392],"nucleoprotein,":[233],"indicating":[234],"provides":[239],"critical":[240],"contacts":[241],"which":[242],"establish":[243,375],"VP35-NP":[245],"interaction.":[246,330],"In":[247],"order":[248],"therapeutically":[250],"regions":[254,305],"promising":[264],"antiviral":[265],"target,":[266],"we":[267],"targeted":[268],"Ebola":[269],"virus":[270],"(EBOV)":[271],"(eVP35)":[273],"aptamer":[275],"selection":[276],"using":[277],"SELEX.":[278],"Select":[279],"aptamers,":[280],"representing":[281],"classes,":[284],"were":[285],"further":[286],"characterized":[287],"based":[288],"interaction":[291],"properties":[292],"eVP35":[294,307,325],"IID.":[295],"results":[297,360],"revealed":[298],"aptamers":[301,317,340,386],"IID":[308],"high":[310,393],"affinity":[311,394],"(10-50":[312],"nM)":[313],"specificity.":[315],"can":[318,341],"compete":[319],"disrupt":[327],"eVP35-NP":[329,337],"Consistent":[331],"antagonize":[336],"interaction,":[338],"select":[339,354],"inhibit":[342],"function":[344],"EBOV":[347,377],"complex":[349],"reconstituted":[350],"expression":[352],"proteins.":[356],"Taken":[357],"together,":[358],"our":[359,368],"serve":[361],"an":[363],"initial":[364],"step":[365],"enhancing":[367],"understanding":[369],"protein-protein":[372],"interactions":[373],"complex,":[379],"support":[381],"identification":[383],"specificity":[396],"have":[398],"capacity":[400],"potentially":[402]},"counts_by_year":[],"updated_date":"2026-09-11T09:22:31.653822","created_date":"2016-06-24T00:00:00"}