{"id":"https://openalex.org/W192160929","doi":null,"title":"Single-Molecule Studies Of Local And Global Nucleic-Acid Dynamics","display_name":"Single-Molecule Studies Of Local And Global Nucleic-Acid Dynamics","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W192160929","mag":"192160929"},"language":"en","primary_location":{"id":"pmh:oai:digitalcommons.wayne.edu:oa_dissertations-1789","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","pdf_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","source":{"id":"https://openalex.org/S171990522","display_name":"Human Biology","issn_l":"0018-7143","issn":["0018-7143","1534-6617"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","erih-plus","jufo-1","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310316469","host_organization_name":"Wayne State University Press","host_organization_lineage":["https://openalex.org/P4310316469"],"host_organization_lineage_names":["Wayne State University Press"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Wayne State University Dissertations","raw_type":"dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013083205","display_name":"Eric M. Patrick","orcid":"https://orcid.org/0000-0003-4915-4257","observed_orcids":["https://orcid.org/0000-0003-4915-4257"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Eric M. Patrick","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4915-4257","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5013083205"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.023099999874830246,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nucleic-acid-dynamics","display_name":"nucleic acid dynamics","score":0.8640000224113464},{"id":"https://openalex.org/keywords/single-molecule-fluorescence","display_name":"single-molecule fluorescence","score":0.7279999852180481},{"id":"https://openalex.org/keywords/2-aminopurine","display_name":"2-aminopurine","score":0.9739999771118164},{"id":"https://openalex.org/keywords/rna-folding","display_name":"RNA folding","score":0.5740000009536743},{"id":"https://openalex.org/keywords/macromolecular-crowding","display_name":"macromolecular crowding","score":0.8429999947547913},{"id":"https://openalex.org/keywords/rna-conformational-change","display_name":"RNA conformational change","score":0.6570000052452087},{"id":"https://openalex.org/keywords/ligand-binding","display_name":"ligand binding","score":0.6620000004768372},{"id":"https://openalex.org/keywords/fluorescence-resonance-energy-transfer","display_name":"fluorescence resonance energy transfer","score":0.5260000228881836}],"concepts":[{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.5726847648620605},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44394034147262573},{"id":"https://openalex.org/C145912823","wikidata":"https://www.wikidata.org/wiki/Q113558","display_name":"Dynamics (music)","level":2,"score":0.43222275376319885},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.410695344209671},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.20919159054756165},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1819053590297699},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:digitalcommons.wayne.edu:oa_dissertations-1789","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","pdf_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","source":{"id":"https://openalex.org/S171990522","display_name":"Human Biology","issn_l":"0018-7143","issn":["0018-7143","1534-6617"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","erih-plus","jufo-1","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310316469","host_organization_name":"Wayne State University Press","host_organization_lineage":["https://openalex.org/P4310316469"],"host_organization_lineage_names":["Wayne State University Press"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Wayne State University Dissertations","raw_type":"dissertation"},{"id":"mag:192160929","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.wayne.edu/cgi/viewcontent.cgi?article=1789&context=oa_dissertations","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:digitalcommons.wayne.edu:oa_dissertations-1789","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","pdf_url":"https://digitalcommons.wayne.edu/oa_dissertations/790","source":{"id":"https://openalex.org/S171990522","display_name":"Human Biology","issn_l":"0018-7143","issn":["0018-7143","1534-6617"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","erih-plus","jufo-1","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310316469","host_organization_name":"Wayne State University Press","host_organization_lineage":["https://openalex.org/P4310316469"],"host_organization_lineage_names":["Wayne State University Press"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Wayne State University Dissertations","raw_type":"dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W192160929.pdf","grobid_xml":"https://content.openalex.org/works/W192160929.grobid-xml"},"referenced_works_count":196,"referenced_works":["https://openalex.org/W68267872","https://openalex.org/W1487724057","https://openalex.org/W1507388704","https://openalex.org/W1509099686","https://openalex.org/W1556324230","https://openalex.org/W1579716589","https://openalex.org/W1584476217","https://openalex.org/W1802567635","https://openalex.org/W1830699959","https://openalex.org/W1964581840","https://openalex.org/W1967689204","https://openalex.org/W1968000756","https://openalex.org/W1968282235","https://openalex.org/W1968869557","https://openalex.org/W1969342142","https://openalex.org/W1971729824","https://openalex.org/W1972101882","https://openalex.org/W1973818768","https://openalex.org/W1974259661","https://openalex.org/W1974370838","https://openalex.org/W1975715632","https://openalex.org/W1975819619","https://openalex.org/W1976608344","https://openalex.org/W1977733607","https://openalex.org/W1979431512","https://openalex.org/W1980945629","https://openalex.org/W1983637347","https://openalex.org/W1986369974","https://openalex.org/W1986414204","https://openalex.org/W1994028292","https://openalex.org/W1994162276","https://openalex.org/W1997756012","https://openalex.org/W1997889144","https://openalex.org/W2000896263","https://openalex.org/W2002969314","https://openalex.org/W2003026778","https://openalex.org/W2003099788","https://openalex.org/W2004518911","https://openalex.org/W2007704891","https://openalex.org/W2007909703","https://openalex.org/W2008382943","https://openalex.org/W2008697605","https://openalex.org/W2009359545","https://openalex.org/W2010460685","https://openalex.org/W2012879790","https://openalex.org/W2013306140","https://openalex.org/W2014095962","https://openalex.org/W2017429695","https://openalex.org/W2017832306","https://openalex.org/W2018012741","https://openalex.org/W2019509622","https://openalex.org/W2021774273","https://openalex.org/W2022208182","https://openalex.org/W2022890528","https://openalex.org/W2023364466","https://openalex.org/W2023447708","https://openalex.org/W2024412145","https://openalex.org/W2025668322","https://openalex.org/W2028617936","https://openalex.org/W2028704745","https://openalex.org/W2028846063","https://openalex.org/W2029667189","https://openalex.org/W2029750840","https://openalex.org/W2030621944","https://openalex.org/W2034728705","https://openalex.org/W2035546741","https://openalex.org/W2035984044","https://openalex.org/W2036116609","https://openalex.org/W2037133353","https://openalex.org/W2037394306","https://openalex.org/W2037413627","https://openalex.org/W2040436341","https://openalex.org/W2041053455","https://openalex.org/W2041139051","https://openalex.org/W2041245086","https://openalex.org/W2043127660","https://openalex.org/W2043411229","https://openalex.org/W2043995349","https://openalex.org/W2044563998","https://openalex.org/W2046258235","https://openalex.org/W2046819990","https://openalex.org/W2048706341","https://openalex.org/W2048912747","https://openalex.org/W2049230793","https://openalex.org/W2049267302","https://openalex.org/W2049681307","https://openalex.org/W2055214702","https://openalex.org/W2058276221","https://openalex.org/W2059306569","https://openalex.org/W2060512844","https://openalex.org/W2060872711","https://openalex.org/W2061922513","https://openalex.org/W2062021590","https://openalex.org/W2062762944","https://openalex.org/W2064175645","https://openalex.org/W2065270611","https://openalex.org/W2065375318","https://openalex.org/W2065986370","https://openalex.org/W2066997160","https://openalex.org/W2068455221","https://openalex.org/W2069122773","https://openalex.org/W2069508494","https://openalex.org/W2069779808","https://openalex.org/W2070293903","https://openalex.org/W2075723202","https://openalex.org/W2075782624","https://openalex.org/W2078334480","https://openalex.org/W2079057579","https://openalex.org/W2079603800","https://openalex.org/W2079632539","https://openalex.org/W2079991169","https://openalex.org/W2080042971","https://openalex.org/W2082419092","https://openalex.org/W2082589966","https://openalex.org/W2083321361","https://openalex.org/W2086401460","https://openalex.org/W2086490175","https://openalex.org/W2088376685","https://openalex.org/W2088892367","https://openalex.org/W2089606505","https://openalex.org/W2091603938","https://openalex.org/W2091829158","https://openalex.org/W2092218280","https://openalex.org/W2092323250","https://openalex.org/W2093700929","https://openalex.org/W2093860423","https://openalex.org/W2100453710","https://openalex.org/W2102032812","https://openalex.org/W2102267264","https://openalex.org/W2102911014","https://openalex.org/W2104247152","https://openalex.org/W2104616613","https://openalex.org/W2106462936","https://openalex.org/W2106798647","https://openalex.org/W2106982820","https://openalex.org/W2110118545","https://openalex.org/W2110298114","https://openalex.org/W2111410489","https://openalex.org/W2111652614","https://openalex.org/W2112333437","https://openalex.org/W2114001343","https://openalex.org/W2117391818","https://openalex.org/W2118974433","https://openalex.org/W2119196820","https://openalex.org/W2120634113","https://openalex.org/W2123416485","https://openalex.org/W2124081058","https://openalex.org/W2126466006","https://openalex.org/W2126516772","https://openalex.org/W2126881800","https://openalex.org/W2127613646","https://openalex.org/W2128868625","https://openalex.org/W2129215648","https://openalex.org/W2131187223","https://openalex.org/W2132976721","https://openalex.org/W2134326554","https://openalex.org/W2134664665","https://openalex.org/W2136440665","https://openalex.org/W2139068617","https://openalex.org/W2140151251","https://openalex.org/W2140885472","https://openalex.org/W2141502463","https://openalex.org/W2141617805","https://openalex.org/W2142844453","https://openalex.org/W2143975644","https://openalex.org/W2145662716","https://openalex.org/W2147488317","https://openalex.org/W2147993766","https://openalex.org/W2148425128","https://openalex.org/W2148715777","https://openalex.org/W2150085801","https://openalex.org/W2151490567","https://openalex.org/W2151842147","https://openalex.org/W2152054441","https://openalex.org/W2153337043","https://openalex.org/W2154194262","https://openalex.org/W2154684482","https://openalex.org/W2154714625","https://openalex.org/W2155376412","https://openalex.org/W2156021317","https://openalex.org/W2156837310","https://openalex.org/W2157867429","https://openalex.org/W2158661016","https://openalex.org/W2160393299","https://openalex.org/W2160594875","https://openalex.org/W2162522101","https://openalex.org/W2163043365","https://openalex.org/W2165410540","https://openalex.org/W2168421163","https://openalex.org/W2169784712","https://openalex.org/W2325945175","https://openalex.org/W2328746876","https://openalex.org/W2332506601","https://openalex.org/W2406148465","https://openalex.org/W4256503849","https://openalex.org/W4297851215"],"related_works":["https://openalex.org/W2326118054","https://openalex.org/W2062944844","https://openalex.org/W2037205953","https://openalex.org/W6926680592","https://openalex.org/W2126330691","https://openalex.org/W40492146","https://openalex.org/W2320337119","https://openalex.org/W657606190","https://openalex.org/W7071669323","https://openalex.org/W2128479875"],"abstract_inverted_index":{"Nucleic":[0],"acids":[1,99],"undergo":[2],"both":[3,146],"global":[4,185],"and":[5,63,100,110,148,177,225,252,259,277,324,342],"local":[6,95,115],"conformational":[7],"changes":[8,66],"that":[9,127,216,282,316],"are":[10,181,197,211,226],"important":[11],"for":[12,152,160],"their":[13,53,157,222,230,263],"function.":[14],"Structural":[15],"studies":[16,280],"have":[17],"over":[18],"the":[19,33,47,60,104,141,194,200,236,244,255,283,291,300,312,319,337],"decades":[20],"been":[21],"invaluable":[22],"in":[23,46,67,70,187,219,234,268,272,299,317],"elucidation":[24],"of":[25,35,42,52,103,107,131,143,213,247,265,285,290,302,307,311],"various":[26],"biomolecular":[27],"mechanisms,":[28],"hence":[29,155,227,253],"contributing":[30],"significantly":[31],"to":[32,82,113,134,221,270],"understanding":[34,41],"biological":[36],"events.":[37],"However,":[38],"a":[39,79,87,168,208,273,308],"clear":[40],"how":[43],"molecules":[44],"function":[45],"cellular":[48,195],"context":[49],"requires":[50],"investigation":[51],"interconversion":[54],"between":[55,339],"multiple":[56],"conformations,":[57],"including":[58],"mapping":[59],"folding":[61,245,257],"landscape":[62,246],"any":[64],"coupled":[65],"conformation.":[68],"Work":[69],"this":[71],"thesis":[72],"focuses":[73],"on":[74,94],"fluorescence":[75,133],"experiments,":[76],"mainly":[77],"at":[78,117],"single-molecule":[80,89,124,136,161],"level":[81],"investigate":[83],"such":[84],"processes.":[85],"First,":[86],"novel":[88],"approach":[90],"is":[91,240],"described":[92],"focusing":[93],"dynamics":[96,116,164],"within":[97,229],"nucleic":[98],"taking":[101],"advantage":[102],"fluorescent":[105],"properties":[106],"2-aminopurine":[108],"(2AP)":[109],"pyrrolo-cytosine":[111],"(PC)":[112],"study":[114],"single":[118],"base":[119],"resolution.":[120],"A":[121],"click":[122],"chemistry-based,":[123],"immobilization":[125],"methodology":[126],"enables":[128],"sufficient":[129,158],"minimization":[130],"background":[132],"allow":[135],"detection":[137],"was":[138],"utilized.":[139],"In":[140],"absence":[142,301],"stacking":[144],"interactions,":[145],"PC":[147],"2AP":[149,166],"fluoresce":[150],"steadily":[151],"several":[153],"seconds":[154],"demonstrating":[156],"photostability":[159],"utilization.":[162],"Local":[163],"using":[165,199],"across":[167],"DNA":[169],"abasic-site":[170],"mimic,":[171],"SAM-1":[172],"riboswitch":[173,204,256,295,320],"binding":[174,327],"pocket":[175],"reorganization,":[176],"tRNAPro":[178],"3'":[179],"end":[180],"reported.":[182],"Additionally,":[183],"RNA":[184],"motions":[186],"conditions":[188],"with":[189],"molecular":[190,286],"crowding,":[191],"which":[192,239],"mimics":[193],"environment,":[196],"reported":[198],"cyclic-diguanylate":[201],"monophosphate":[202],"(c-di-GMP)":[203],"aptamer":[205,296],"domain":[206],"as":[207],"model.":[209],"Riboswitches":[210],"examples":[212],"gene-regulating":[214],"elements":[215],"normally":[217],"act":[218],"cis":[220],"host":[223],"mRNAs":[224],"located":[228],"5'":[231],"untranslated":[232],"regions.":[233],"vivo,":[235],"intracellular":[237],"physiology,":[238],"highly":[241],"crowded,":[242],"dictates":[243],"RNAs.":[248],"Macromolecular":[249],"crowding":[250,287],"influences,":[251],"alters,":[254],"pathway,":[258],"subsequently":[260],"may":[261,328,334],"change":[262],"mode":[264],"ligand/substrate":[266],"recognition":[267],"comparison":[269],"experiments":[271],"non-crowded":[274],"medium.":[275],"Single-molecule":[276],"steady-state":[278],"FRET":[279],"show":[281],"presence":[284],"enhances":[288],"docking":[289],"ubiquitous":[292],"bacterial":[293],"c-di-GMP":[294],"region":[297],"even":[298],"it's":[303],"metabolite.":[304],"The":[305],"adoption":[306],"structure":[309],"reminiscent":[310],"ligand-bound":[313],"form":[314],"implies":[315],"vivo":[318],"can":[321],"fully":[322],"fold,":[323],"hence,":[325],"ligand":[326],"only":[329],"require":[330],"minimal":[331],"rearrangements.":[332],"This":[333],"further":[335],"decrease":[336],"time":[338],"ligand-recognition,":[340],"interaction,":[341],"transcription":[343],"enhancement.":[344]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}