{"id":"https://openalex.org/W191234557","doi":"https://doi.org/10.2144/04373st01","title":"Polyhydroxybutyrate-Enhanced Transformation of Log-Phase Escherichia Coli","display_name":"Polyhydroxybutyrate-Enhanced Transformation of Log-Phase Escherichia Coli","publication_year":2004,"publication_date":"2004-09-01","ids":{"openalex":"https://openalex.org/W191234557","doi":"https://doi.org/10.2144/04373st01","mag":"191234557","pmid":"https://pubmed.ncbi.nlm.nih.gov/15470891"},"language":"en","primary_location":{"id":"doi:10.2144/04373st01","doi":"https://doi.org/10.2144/04373st01","is_oa":true,"landing_page_url":"https://doi.org/10.2144/04373st01","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/04373st01","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033294776","display_name":"Charles J. Addison","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles J. Addison","raw_affiliation_strings":["Michigan State University, East Lansing, MI, USA","Michigan State University, East Lansing, MI 48824 USA ()"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Michigan State University, East Lansing, MI, USA","institution_ids":["https://openalex.org/I87216513"]},{"raw_affiliation_string":"Michigan State University, East Lansing, MI 48824 USA ()","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"middle","author":{"id":null,"display_name":"S. Haihua Chu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S. Haihua Chu","raw_affiliation_strings":["Michigan State University, East Lansing, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Michigan State University, East Lansing, MI, USA","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110204882","display_name":"Rosetta N. Reusch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rosetta N. Reusch","raw_affiliation_strings":["Michigan State University, East Lansing, MI, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Michigan State University, East Lansing, MI, USA","institution_ids":["https://openalex.org/I87216513"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I87216513"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.4375,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.58717474,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"37","issue":"3","first_page":"376","last_page":"382"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacterial-transformation","display_name":"bacterial transformation","score":0.703000009059906},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9639999866485596},{"id":"https://openalex.org/keywords/polyhydroxybutyrate","display_name":"polyhydroxybutyrate","score":0.9240000247955322},{"id":"https://openalex.org/keywords/log-phase-cells","display_name":"log-phase cells","score":0.765999972820282},{"id":"https://openalex.org/keywords/plasmid-dna-uptake","display_name":"plasmid DNA uptake","score":0.8370000123977661},{"id":"https://openalex.org/keywords/dimethyl-sulfoxide","display_name":"dimethyl sulfoxide","score":0.9079999923706055},{"id":"https://openalex.org/keywords/competence","display_name":"competence","score":0.5640000104904175},{"id":"https://openalex.org/keywords/thermal-shock","display_name":"thermal shock","score":0.7680000066757202},{"id":"https://openalex.org/keywords/factorial-design-optimization","display_name":"factorial design optimization","score":0.6340000033378601},{"id":"https://openalex.org/keywords/automated-transformation","display_name":"automated transformation","score":0.5879999995231628}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7882161140441895},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.704839289188385},{"id":"https://openalex.org/C2778452553","wikidata":"https://www.wikidata.org/wiki/Q407927","display_name":"Dimethyl sulfoxide","level":2,"score":0.5636235475540161},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5024735927581787},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.49831724166870117},{"id":"https://openalex.org/C178050479","wikidata":"https://www.wikidata.org/wiki/Q7834084","display_name":"Transformation efficiency","level":5,"score":0.4947502017021179},{"id":"https://openalex.org/C2780849362","wikidata":"https://www.wikidata.org/wiki/Q424469","display_name":"Polyhydroxybutyrate","level":3,"score":0.4898349940776825},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4741375148296356},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.4581001102924347},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40240955352783203},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3853828012943268},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3426256775856018},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.25138184428215027},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20332831144332886},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.19916284084320068},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.10508990287780762},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000094122","descriptor_name":"Polyhydroxybutyrates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006885","descriptor_name":"Hydroxybutyrates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007202","descriptor_name":"Indicators and Reagents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011091","descriptor_name":"Polyesters","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014169","descriptor_name":"Transformation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.2144/04373st01","doi":"https://doi.org/10.2144/04373st01","is_oa":true,"landing_page_url":"https://doi.org/10.2144/04373st01","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:15470891","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15470891","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:c7e32f9f89d746fdaa2878b68229814f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/c7e32f9f89d746fdaa2878b68229814f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 37, Iss 3, Pp 376-382 (2004)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/04373st01","doi":"https://doi.org/10.2144/04373st01","is_oa":true,"landing_page_url":"https://doi.org/10.2144/04373st01","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310016","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W33546104","https://openalex.org/W184045801","https://openalex.org/W628063787","https://openalex.org/W1507752894","https://openalex.org/W1512810258","https://openalex.org/W1535420773","https://openalex.org/W1996730821","https://openalex.org/W2020888020","https://openalex.org/W2043672744","https://openalex.org/W2106398055","https://openalex.org/W2109286270","https://openalex.org/W2132417094","https://openalex.org/W2144530181","https://openalex.org/W2218155072","https://openalex.org/W2331721152","https://openalex.org/W2970546371","https://openalex.org/W3140959177"],"related_works":["https://openalex.org/W2083714888","https://openalex.org/W3208595378","https://openalex.org/W4235958518","https://openalex.org/W1975920595","https://openalex.org/W2012131407","https://openalex.org/W1999851893","https://openalex.org/W198598427","https://openalex.org/W3048593772","https://openalex.org/W4286852520","https://openalex.org/W3032113618"],"abstract_inverted_index":{"Transformation":[0],"of":[1,54,61,145],"Escherichia":[2],"coli":[3,48],"plays":[4],"an":[5],"important":[6],"role":[7],"in":[8,163],"recombinant":[9],"DNA":[10,63,74],"technology.":[11],"Most":[12],"current":[13],"transformation":[14,34,147,167],"protocols":[15],"require":[16],"that":[17,86,155],"the":[18,59,112,146,156],"cells":[19],"be":[20,159],"treated":[21],"to":[22,57,72],"attain":[23],"a":[24,42,133],"particular":[25],"physiological":[26],"state":[27],"known":[28],"as":[29],"\"competence,\"":[30],"and":[31,37,66,69,91,96,101,106],"this":[32],"makes":[33],"procedures":[35],"lengthy":[36],"arduous.":[38],"Here":[39],"we":[40],"describe":[41],"protocol":[43,77,114],"for":[44,139,161],"transforming":[45],"log-phase":[46,125],"E.":[47],"using":[49,80],"dimethyl":[50],"sulfoxide":[51],"(DMSO)":[52],"solutions":[53],"poly-(R)-3-hydroxybutyrate":[55],"(PHB)":[56],"facilitate":[58],"transfer":[60],"plasmid":[62],"into":[64],"cells,":[65],"certain":[67],"reagents":[68],"temperature":[70],"shocks":[71],"promote":[73],"uptake.":[75],"The":[76,142],"was":[78],"optimized":[79,113],"factorial":[81],"design":[82],"techniques":[83],"across":[84],"variables":[85],"included":[87],"PHB":[88],"molecular":[89],"weight":[90],"concentration,":[92,94],"DMSO":[93],"monovalent":[95],"divalent":[97],"salts,":[98],"glucose,":[99],"cold":[100],"heat":[102],"shocks,":[103],"cell":[104,126],"density,":[105],"pH.":[107],"Using":[108],"10":[109],"ng":[110],"DNA,":[111],"produces":[115],"approximately":[116,129],"1000":[117],"colony-forming":[118],"units":[119],"(CFUs)":[120],"from":[121,132],"100":[122],"microL":[123,153],"early":[124],"culture":[127],"or":[128,165],"300":[130],"CFU":[131],"21-24":[134],"h":[135],"single":[136],"colony,":[137],"sufficient":[138],"many":[140],"applications.":[141],"total":[143],"volume":[144],"reaction":[148],"mixture":[149],"is":[150],"only":[151],"150":[152],"suggesting":[154],"procedure":[157],"may":[158],"adapted":[160],"use":[162],"microplates":[164],"automated":[166],"technologies.":[168]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
