{"id":"https://openalex.org/W1908521937","doi":"https://doi.org/10.1128/iai.64.3.739-743.1996","title":"Antibody-mediated inhibition of Aedes aegypti midgut trypsins blocks sporogonic development of Plasmodium gallinaceum","display_name":"Antibody-mediated inhibition of Aedes aegypti midgut trypsins blocks sporogonic development of Plasmodium gallinaceum","publication_year":1996,"publication_date":"1996-03-01","ids":{"openalex":"https://openalex.org/W1908521937","doi":"https://doi.org/10.1128/iai.64.3.739-743.1996","mag":"1908521937","pmid":"https://pubmed.ncbi.nlm.nih.gov/8641775","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/173831"},"language":"en","primary_location":{"id":"doi:10.1128/iai.64.3.739-743.1996","doi":"https://doi.org/10.1128/iai.64.3.739-743.1996","is_oa":true,"landing_page_url":"https://doi.org/10.1128/iai.64.3.739-743.1996","pdf_url":"https://iai.asm.org/content/iai/64/3/739.full.pdf","source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W1908521937"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://iai.asm.org/content/iai/64/3/739.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"M Shahabuddin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M Shahabuddin","raw_affiliation_strings":["Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA","institution_ids":["https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082887357","display_name":"Francisco Jos\u00e9 Alves Lemos","orcid":"https://orcid.org/0000-0002-1435-4104","observed_orcids":["https://orcid.org/0000-0002-1435-4104"]},"institutions":[{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F J Lemos","raw_affiliation_strings":["Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA","institution_ids":["https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011187406","display_name":"David C. Kaslow","orcid":"https://orcid.org/0000-0003-3557-383X","observed_orcids":["https://orcid.org/0000-0003-3557-383X"]},"institutions":[{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D C Kaslow","raw_affiliation_strings":["Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA","institution_ids":["https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5001978382","display_name":"Marcelo Jacobs\u2010Lorena","orcid":"https://orcid.org/0000-0003-0449-432X","observed_orcids":["https://orcid.org/0000-0003-0449-432X"]},"institutions":[{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M Jacobs-Lorena","raw_affiliation_strings":["Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0425, USA","institution_ids":["https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210134534"],"apc_list":null,"apc_paid":null,"fwci":5.9218,"has_fulltext":false,"cited_by_count":68,"citation_normalized_percentile":{"value":0.95709777,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"64","issue":"3","first_page":"739","last_page":"743"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12178","display_name":"Bird parasitology and diseases","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aedes-aegypti","display_name":"Aedes aegypti","score":0.8939999938011169},{"id":"https://openalex.org/keywords/plasmodium-gallinaceum","display_name":"Plasmodium gallinaceum","score":0.9750000238418579},{"id":"https://openalex.org/keywords/peritrophic-matrix","display_name":"peritrophic matrix","score":0.8199999928474426},{"id":"https://openalex.org/keywords/chitinase","display_name":"chitinase","score":0.9739999771118164},{"id":"https://openalex.org/keywords/sporogony","display_name":"sporogony","score":0.8330000042915344},{"id":"https://openalex.org/keywords/malaria-transmission-blocking","display_name":"malaria transmission blocking","score":0.5899999737739563},{"id":"https://openalex.org/keywords/mosquito-midgut","display_name":"mosquito midgut","score":0.8169999718666077},{"id":"https://openalex.org/keywords/blood-meal","display_name":"blood meal","score":0.40299999713897705}],"concepts":[{"id":"https://openalex.org/C2779190360","wikidata":"https://www.wikidata.org/wiki/Q7201888","display_name":"Plasmodium gallinaceum","level":5,"score":0.9359545111656189},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8431491851806641},{"id":"https://openalex.org/C2777775583","wikidata":"https://www.wikidata.org/wiki/Q1148004","display_name":"Aedes aegypti","level":3,"score":0.8294236660003662},{"id":"https://openalex.org/C150149183","wikidata":"https://www.wikidata.org/wiki/Q1940081","display_name":"Midgut","level":3,"score":0.6718583703041077},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6675357222557068},{"id":"https://openalex.org/C106358424","wikidata":"https://www.wikidata.org/wiki/Q1662346","display_name":"Infectivity","level":3,"score":0.5966904163360596},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5907418131828308},{"id":"https://openalex.org/C2778675580","wikidata":"https://www.wikidata.org/wiki/Q614956","display_name":"Plasmodium (life cycle)","level":3,"score":0.5166335701942444},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4985349178314209},{"id":"https://openalex.org/C2778371730","wikidata":"https://www.wikidata.org/wiki/Q311383","display_name":"Plasmodium falciparum","level":3,"score":0.4541766345500946},{"id":"https://openalex.org/C71928629","wikidata":"https://www.wikidata.org/wiki/Q804206","display_name":"Parasite hosting","level":2,"score":0.41974854469299316},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.32563427090644836},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.24054789543151855},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.22113674879074097},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.22000089287757874},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2045949101448059},{"id":"https://openalex.org/C99389464","wikidata":"https://www.wikidata.org/wiki/Q3319949","display_name":"Gametocyte","level":4,"score":0.18355289101600647},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1264924705028534},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000330","descriptor_name":"Aedes","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002688","descriptor_name":"Chitinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008288","descriptor_name":"Malaria","qualifier_ui":"Q000635","qualifier_name":"transmission","is_major_topic":true},{"descriptor_ui":"D010964","descriptor_name":"Plasmodium gallinaceum","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/iai.64.3.739-743.1996","doi":"https://doi.org/10.1128/iai.64.3.739-743.1996","is_oa":true,"landing_page_url":"https://doi.org/10.1128/iai.64.3.739-743.1996","pdf_url":"https://iai.asm.org/content/iai/64/3/739.full.pdf","source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:149726","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC173831","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:8641775","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8641775","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and immunity","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:173831","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC173831/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infect Immun","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1128/iai.64.3.739-743.1996","doi":"https://doi.org/10.1128/iai.64.3.739-743.1996","is_oa":true,"landing_page_url":"https://doi.org/10.1128/iai.64.3.739-743.1996","pdf_url":"https://iai.asm.org/content/iai/64/3/739.full.pdf","source":{"id":"https://openalex.org/S128227336","display_name":"Infection and Immunity","issn_l":"0019-9567","issn":["0019-9567","1070-6313","1098-5522"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Infection and Immunity","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7246000170707703}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1908521937.pdf"},"referenced_works_count":15,"referenced_works":["https://openalex.org/W150596933","https://openalex.org/W605026253","https://openalex.org/W1516594363","https://openalex.org/W1584868072","https://openalex.org/W1965439340","https://openalex.org/W1973190704","https://openalex.org/W1984849164","https://openalex.org/W1987911729","https://openalex.org/W1996708499","https://openalex.org/W2060277682","https://openalex.org/W2079396741","https://openalex.org/W2093389993","https://openalex.org/W2093566013","https://openalex.org/W2321690467","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W1984849164","https://openalex.org/W4237786348","https://openalex.org/W2095390288","https://openalex.org/W2009485252","https://openalex.org/W1987911729","https://openalex.org/W1979307172","https://openalex.org/W2148962899","https://openalex.org/W2006601636","https://openalex.org/W2328536519","https://openalex.org/W1974336670"],"abstract_inverted_index":{"The":[0],"peritrophic":[1],"matrix":[2],"(PM)":[3],"that":[4,23,34,46,52,155],"forms":[5],"around":[6],"a":[7,11,31,111,176,183],"blood":[8,128],"meal":[9],"is":[10,48],"potential":[12],"barrier":[13],"of":[14,37,83,100,135,144,175],"Plasmodium":[15,28,55,87,140],"development":[16],"in":[17],"mosquitoes.":[18],"Previously,":[19],"we":[20,44,96],"have":[21],"shown":[22],"to":[24,172],"traverse":[25],"the":[26,49,54,61,84,98,104,130,156,166,173],"PM,":[27],"ookinetes":[29],"secrete":[30],"prochitinase":[32,161],"and":[33,70,77,81,116,163],"an":[35,126],"inhibitor":[36],"chitinase":[38],"blocks":[39],"further":[40],"parasite":[41,105,167],"development.":[42],"Here":[43],"report":[45],"it":[47],"mosquito":[50,119,177],"trypsin":[51,68,114,120],"activates":[53],"prochitinase.":[56],"Trypsin":[57],"was":[58],"identified":[59],"as":[60,182],"chitinase-activating":[62],"enzyme":[63],"by":[64,86,92,139,149,159],"two":[65],"criteria:":[66],"(i)":[67],"activity":[69,72,80],"activating":[71,79],"comigrated":[73],"on":[74,103,165],"one-dimensional":[75],"gels,":[76],"(ii)":[78],"penetration":[82],"PM":[85],"parasites":[88],"were":[89],"both":[90],"hindered":[91],"trypsin-specific":[93],"inhibitors.":[94],"Subsequently,":[95],"examined":[97],"effect":[99],"antitrypsin":[101,131],"antibodies":[102,132,157],"life":[106],"cycle.":[107],"Antibodies":[108],"prepared":[109],"against":[110],"recombinant":[112],"blackfly":[113],"effectively":[115],"specifically":[117],"inhibited":[118],"activity.":[121],"Moreover,":[122],"when":[123],"incorporated":[124],"into":[125],"infective":[127],"meal,":[129],"blocked":[133],"infectivity":[134,145],"Aedes":[136],"aegypti":[137],"mosquitoes":[138],"gallinaceum.":[141],"This":[142,169],"block":[143],"could":[146],"be":[147],"reversed":[148],"exogenously":[150],"provided":[151],"chitinase,":[152],"strongly":[153],"suggesting":[154],"act":[158],"inhibiting":[160],"activation":[162],"not":[164],"itself.":[168],"work":[170],"led":[171],"identification":[174],"antigen,":[178],"i.e.,":[179],"midgut":[180],"trypsin,":[181],"novel":[184],"target":[185],"for":[186],"blocking":[187],"malaria":[188],"transmission.":[189]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}