{"id":"https://openalex.org/W1908504338","doi":"https://doi.org/10.4049/jimmunol.145.12.4115","title":"Induction of gene expression by IL-1 in NIH 3T3 cells. Possible requirement of protein kinase C activity and independence from arachidonic acid metabolism.","display_name":"Induction of gene expression by IL-1 in NIH 3T3 cells. Possible requirement of protein kinase C activity and independence from arachidonic acid metabolism.","publication_year":1990,"publication_date":"1990-12-01","ids":{"openalex":"https://openalex.org/W1908504338","doi":"https://doi.org/10.4049/jimmunol.145.12.4115","mag":"1908504338","pmid":"https://pubmed.ncbi.nlm.nih.gov/2124235"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.145.12.4115","doi":"https://doi.org/10.4049/jimmunol.145.12.4115","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.145.12.4115","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028520464","display_name":"Daniele Donati","orcid":"https://orcid.org/0000-0002-4791-8762","observed_orcids":["https://orcid.org/0000-0002-4791-8762"]},"institutions":[{"id":"https://openalex.org/I102064193","display_name":"University of Siena","ror":"https://ror.org/01tevnk56","country_code":"IT","type":"education","lineage":["https://openalex.org/I102064193"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"D Donati","raw_affiliation_strings":["Laboratory of Pharmacology, Sclavo Research Centre, University of Siena , Siena ,","Laboratory of Pharmacology, Sclavo Research Centre, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, University of Siena , Siena ,","institution_ids":["https://openalex.org/I102064193"]},{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068478900","display_name":"C. T. Baldari","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102064193","display_name":"University of Siena","ror":"https://ror.org/01tevnk56","country_code":"IT","type":"education","lineage":["https://openalex.org/I102064193"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"C T Baldari","raw_affiliation_strings":["Department of Evolutionary Biology, University of Siena , Siena ,","Laboratory of Pharmacology, Sclavo Research Centre, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Evolutionary Biology, University of Siena , Siena ,","institution_ids":["https://openalex.org/I102064193"]},{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083660696","display_name":"G Macchia","orcid":"https://orcid.org/0000-0002-8795-5883","observed_orcids":["https://orcid.org/0000-0002-8795-5883"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G Macchia","raw_affiliation_strings":["Laboratory of Molecular Biology, Sclavo Research Centre , Siena ,","Laboratory of Pharmacology, Sclavo Research Centre, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Sclavo Research Centre , Siena ,","institution_ids":[]},{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113587260","display_name":"Annalisa Massone","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Massone","raw_affiliation_strings":["Laboratory of Molecular Biology, Sclavo Research Centre , Siena ,","Laboratory of Pharmacology, Sclavo Research Centre, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Sclavo Research Centre , Siena ,","institution_ids":[]},{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050822412","display_name":"John L. Telford","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J L Telford","raw_affiliation_strings":["Laboratory of Molecular Biology, Sclavo Research Centre , Siena ,","Laboratory of Pharmacology, Sclavo Research Centre, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Sclavo Research Centre , Siena ,","institution_ids":[]},{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, Italy","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018449992","display_name":"Luca Parente","orcid":"https://orcid.org/0009-0008-3395-8510","observed_orcids":["https://orcid.org/0009-0008-3395-8510"]},"institutions":[{"id":"https://openalex.org/I102064193","display_name":"University of Siena","ror":"https://ror.org/01tevnk56","country_code":"IT","type":"education","lineage":["https://openalex.org/I102064193"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"L Parente","raw_affiliation_strings":["Laboratory of Pharmacology, Sclavo Research Centre, University of Siena , Siena ,","Laboratory of Pharmacology, Sclavo Research Centre, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, University of Siena , Siena ,","institution_ids":["https://openalex.org/I102064193"]},{"raw_affiliation_string":"Laboratory of Pharmacology, Sclavo Research Centre, Italy","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":2.4204,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.87627871,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"145","issue":"12","first_page":"4115","last_page":"4120"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.6963000297546387,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.6963000297546387,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11503","display_name":"Cytokine Signaling Pathways and Interactions","score":0.10369999706745148,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.08500000089406967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/interleukin-1","display_name":"interleukin-1","score":0.9760000109672546},{"id":"https://openalex.org/keywords/gene-induction","display_name":"gene induction","score":0.6520000100135803},{"id":"https://openalex.org/keywords/protein-kinase-c","display_name":"protein kinase C","score":0.9900000095367432},{"id":"https://openalex.org/keywords/arachidonic-acid-metabolism","display_name":"arachidonic acid metabolism","score":0.9980000257492065},{"id":"https://openalex.org/keywords/prostaglandin-e2-release","display_name":"prostaglandin E2 release","score":0.9670000076293945},{"id":"https://openalex.org/keywords/tnf-alpha","display_name":"TNF-\u03b1","score":0.6470000147819519},{"id":"https://openalex.org/keywords/cytokine-synergy","display_name":"cytokine synergy","score":0.7039999961853027},{"id":"https://openalex.org/keywords/chloramphenicol-acetyltransferase-reporter","display_name":"chloramphenicol acetyltransferase reporter","score":0.8479999899864197},{"id":"https://openalex.org/keywords/fluocinolone","display_name":"fluocinolone","score":0.8410000205039978},{"id":"https://openalex.org/keywords/phorbol-myristate-acetate","display_name":"phorbol myristate acetate","score":0.8270000219345093},{"id":"https://openalex.org/keywords/diacylglycerol-kinase","display_name":"diacylglycerol kinase","score":0.8100000023841858},{"id":"https://openalex.org/keywords/sphingosine","display_name":"sphingosine","score":0.8289999961853027},{"id":"https://openalex.org/keywords/h7-inhibitor","display_name":"H7 inhibitor","score":0.6140000224113464},{"id":"https://openalex.org/keywords/nih-3t3-cells","display_name":"NIH3T3 cells","score":0.8519999980926514}],"concepts":[{"id":"https://openalex.org/C195794163","wikidata":"https://www.wikidata.org/wiki/Q420877","display_name":"Protein kinase C","level":3,"score":0.6566002368927002},{"id":"https://openalex.org/C36020004","wikidata":"https://www.wikidata.org/wiki/Q106951674","display_name":"Diacylglycerol kinase","level":4,"score":0.5223187804222107},{"id":"https://openalex.org/C2779276759","wikidata":"https://www.wikidata.org/wiki/Q412747","display_name":"Forskolin","level":3,"score":0.513105034828186},{"id":"https://openalex.org/C2779084600","wikidata":"https://www.wikidata.org/wiki/Q5957181","display_name":"Staurosporine","level":4,"score":0.4685222804546356},{"id":"https://openalex.org/C2776746612","wikidata":"https://www.wikidata.org/wiki/Q68153243","display_name":"3T3 cells","level":4,"score":0.46671149134635925},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4648771286010742},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.46309852600097656},{"id":"https://openalex.org/C2778078955","wikidata":"https://www.wikidata.org/wiki/Q407699","display_name":"Arachidonic acid","level":3,"score":0.45223870873451233},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4452313184738159},{"id":"https://openalex.org/C2780730277","wikidata":"https://www.wikidata.org/wiki/Q24744759","display_name":"Chloramphenicol acetyltransferase","level":5,"score":0.41657504439353943},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.37550652027130127},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3426138758659363},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.33780786395072937},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.31940141320228577},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26315850019454956},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2177615463733673},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.21416836977005005},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.14018797874450684},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08675208687782288}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001095","descriptor_name":"Arachidonic Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007375","descriptor_name":"Interleukin-1","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D011493","descriptor_name":"Protein Kinase C","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D011493","descriptor_name":"Protein Kinase C","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D011744","descriptor_name":"Pyrimidinones","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013755","descriptor_name":"Tetradecanoylphorbol Acetate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013844","descriptor_name":"Thiazoles","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D014409","descriptor_name":"Tumor Necrosis Factor-alpha","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015232","descriptor_name":"Dinoprostone","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D016718","descriptor_name":"Arachidonic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016859","descriptor_name":"Lipoxygenase Inhibitors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016861","descriptor_name":"Cyclooxygenase Inhibitors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.4049/jimmunol.145.12.4115","doi":"https://doi.org/10.4049/jimmunol.145.12.4115","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.145.12.4115","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},{"id":"pmid:2124235","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2124235","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunology (Baltimore, Md. : 1950)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2070833091","https://openalex.org/W1603149709","https://openalex.org/W1933220465","https://openalex.org/W2085782807","https://openalex.org/W2036612597","https://openalex.org/W2098346866","https://openalex.org/W1972234924","https://openalex.org/W2144282829","https://openalex.org/W2024722044","https://openalex.org/W2039360786"],"abstract_inverted_index":{"NIH":[0,198],"3T3":[1,199],"fibroblasts":[2],"were":[3,35,98,151],"transfected":[4],"with":[5,95],"the":[6,11,14,33,67,201],"chloramphenicol-acetyltransferase":[7],"(CAT)":[8],"gene":[9,213,227],"under":[10],"control":[12,38,172],"of":[13,66,80,203,242],"SV40":[15],"early":[16],"promoter,":[17],"which":[18],"can":[19],"be":[20],"stimulated":[21,40,56,85],"by":[22,182,190,206,236],"IL-1.":[23],"CAT":[24,58,90,102,141,175],"activity":[25,59,103,219],"in":[26,32,37,43,71,197,211,239],"cell":[27,41],"lysates":[28],"and":[29,39,50,60,134,147,159,229],"PGE2":[30,61,76,86,107,178],"release":[31,87,179],"supernatants":[34],"measured":[36],"cultures":[42],"parallel.":[44],"Human":[45],"IL-1":[46,207],"beta":[47],"(180":[48],"pM)":[49],"human":[51],"rTNF-alpha":[52],"(3":[53],"nM)":[54,121],"significantly":[55],"both":[57,126,154],"release.":[62,77,108],"The":[63,78,117,192],"combined":[64],"incubation":[65],"two":[68],"cytokines":[69],"resulted":[70],"a":[72,162,237],"synergistic":[73],"effect":[74,100,186],"on":[75,101,153],"addition":[79],"AA":[81,96,204,234],"(30":[82],"microM)":[83,111,133,137,146,150,158],"greatly":[84],"without":[88,99],"affecting":[89],"activity.":[91,176,245],"Similarly,":[92],"drugs":[93],"interfering":[94],"metabolism":[97,205,235],"although":[104,184],"profoundly":[105],"reducing":[106],"Forskolin":[109],"(0.1":[110],"did":[112,168],"not":[113,169,188,209,223],"modify":[114,170],"either":[115,171],"parameter.":[116],"glucocorticoid":[118],"fluocinolone":[119],"(20":[120],"was":[122,180,187],"able":[123],"to":[124],"decrease":[125],"parameters.":[127,155],"Protein":[128],"kinase":[129,164,217,244],"inhibitors":[130],"H7":[131],"(5-50":[132,145],"sphingosine":[135],"(50":[136,149],"inhibited":[138,189],"only":[139],"IL-1-induced":[140,174,212,226],"activity,":[142],"whereas":[143],"H8":[144],"HA1004":[148],"ineffective":[152],"PMA":[156,231],"(0.5":[157],"R59":[160],"022,":[161],"diacylglycerol":[163],"inhibitor":[165],"(10":[166],"microM),":[167],"or":[173],"IL-1-stimulated":[177],"potentiated":[181],"PMA,":[183],"this":[185],"H7.":[191],"data":[193],"suggest":[194],"that:":[195],"1)":[196],"cells":[200],"activation":[202],"is":[208,220],"involved":[210],"expression;":[214,228],"2)":[215],"protein":[216,243],"C":[218],"required":[221],"but":[222],"sufficient":[224],"for":[225],"3)":[230],"may":[232],"stimulate":[233],"mechanism":[238],"part":[240],"independent":[241]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}