{"id":"https://openalex.org/W1908212314","doi":"https://doi.org/10.1046/j.1365-2613.1998.00083.x","title":"The current status of primary hepatocyte culture","display_name":"The current status of primary hepatocyte culture","publication_year":1998,"publication_date":"1998-12-01","ids":{"openalex":"https://openalex.org/W1908212314","doi":"https://doi.org/10.1046/j.1365-2613.1998.00083.x","mag":"1908212314","pmid":"https://pubmed.ncbi.nlm.nih.gov/10319020","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3220367"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2613.1998.00083.x","doi":"https://doi.org/10.1046/j.1365-2613.1998.00083.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2613.1998.00083.x","pdf_url":null,"source":{"id":"https://openalex.org/S82103187","display_name":"International Journal of Experimental Pathology","issn_l":"0959-9673","issn":["0959-9673","1365-2613"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Experimental Pathology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3220367","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017018776","display_name":"Toshihiro Mitaka","orcid":"https://orcid.org/0000-0001-9467-4871","observed_orcids":["https://orcid.org/0000-0001-9467-4871"]},"institutions":[{"id":"https://openalex.org/I141882310","display_name":"Sapporo Medical University","ror":"https://ror.org/01h7cca57","country_code":"JP","type":"education","lineage":["https://openalex.org/I141882310"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Toshihiro Mitaka","raw_affiliation_strings":["Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Japan. tmitaka@cc.sapmed.ac.jp","Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Japan. tmitaka@cc.sapmed.ac.jp","institution_ids":["https://openalex.org/I141882310"]},{"raw_affiliation_string":"Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Japan","institution_ids":["https://openalex.org/I141882310"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5017018776"],"corresponding_institution_ids":["https://openalex.org/I141882310"],"apc_list":{"value":3730,"currency":"USD","value_usd":3730},"apc_paid":null,"fwci":2.4731,"has_fulltext":false,"cited_by_count":116,"citation_normalized_percentile":{"value":0.89135992,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"79","issue":"6","first_page":"393","last_page":"409"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10999","display_name":"Organ Transplantation Techniques and Outcomes","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11465","display_name":"Drug-Induced Hepatotoxicity and Protection","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/primary-hepatocyte-culture","display_name":"primary hepatocyte culture","score":0.9440000057220459},{"id":"https://openalex.org/keywords/hepatocyte-proliferation","display_name":"hepatocyte proliferation","score":0.9610000252723694},{"id":"https://openalex.org/keywords/nicotinamide","display_name":"nicotinamide","score":0.9290000200271606},{"id":"https://openalex.org/keywords/dimethyl-sulfoxide","display_name":"dimethyl sulfoxide","score":0.9520000219345093},{"id":"https://openalex.org/keywords/hepatocyte-differentiation","display_name":"hepatocyte differentiation","score":0.9520000219345093},{"id":"https://openalex.org/keywords/liver-growth","display_name":"liver growth","score":0.6980000138282776}],"concepts":[{"id":"https://openalex.org/C2776200302","wikidata":"https://www.wikidata.org/wiki/Q827450","display_name":"Hepatocyte","level":3,"score":0.8290425539016724},{"id":"https://openalex.org/C2994367422","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Primary culture","level":3,"score":0.7204757928848267},{"id":"https://openalex.org/C2779728014","wikidata":"https://www.wikidata.org/wiki/Q192423","display_name":"Nicotinamide","level":3,"score":0.70802903175354},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6315836310386658},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6125876307487488},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5647830963134766},{"id":"https://openalex.org/C2777977315","wikidata":"https://www.wikidata.org/wiki/Q7243056","display_name":"Primary (astronomy)","level":2,"score":0.5049133896827698},{"id":"https://openalex.org/C2776996007","wikidata":"https://www.wikidata.org/wiki/Q2399112","display_name":"Hepatocyte growth factor","level":3,"score":0.48558157682418823},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26269370317459106},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1046/j.1365-2613.1998.00083.x","doi":"https://doi.org/10.1046/j.1365-2613.1998.00083.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2613.1998.00083.x","pdf_url":null,"source":{"id":"https://openalex.org/S82103187","display_name":"International Journal of Experimental Pathology","issn_l":"0959-9673","issn":["0959-9673","1365-2613"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Experimental Pathology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3220367","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3220367","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Exp Pathol","raw_type":"Text"},{"id":"pmid:10319020","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10319020","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of experimental pathology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:3220367","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3220367","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Exp Pathol","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":176,"referenced_works":["https://openalex.org/W64775550","https://openalex.org/W109562680","https://openalex.org/W110259188","https://openalex.org/W124367642","https://openalex.org/W143089095","https://openalex.org/W186151347","https://openalex.org/W200379842","https://openalex.org/W318334032","https://openalex.org/W594356966","https://openalex.org/W868238619","https://openalex.org/W1224253673","https://openalex.org/W1504270866","https://openalex.org/W1516498539","https://openalex.org/W1529304947","https://openalex.org/W1561865903","https://openalex.org/W1562841260","https://openalex.org/W1563645938","https://openalex.org/W1565037888","https://openalex.org/W1590655041","https://openalex.org/W1604066767","https://openalex.org/W1606465625","https://openalex.org/W1616193782","https://openalex.org/W1702287731","https://openalex.org/W1704094093","https://openalex.org/W1793177151","https://openalex.org/W1821053548","https://openalex.org/W1833862072","https://openalex.org/W1870688656","https://openalex.org/W1892608609","https://openalex.org/W1924905604","https://openalex.org/W1967025210","https://openalex.org/W1970175603","https://openalex.org/W1970938899","https://openalex.org/W1972274914","https://openalex.org/W1974128758","https://openalex.org/W1974854390","https://openalex.org/W1975042363","https://openalex.org/W1978040580","https://openalex.org/W1978348250","https://openalex.org/W1978381523","https://openalex.org/W1978944567","https://openalex.org/W1980273449","https://openalex.org/W1980544949","https://openalex.org/W1981305073","https://openalex.org/W1981521685","https://openalex.org/W1983352401","https://openalex.org/W1983524666","https://openalex.org/W1983796700","https://openalex.org/W1983883945","https://openalex.org/W1988300335","https://openalex.org/W1989297142","https://openalex.org/W1991501430","https://openalex.org/W1991506429","https://openalex.org/W1991972774","https://openalex.org/W1994006745","https://openalex.org/W1995612506","https://openalex.org/W1996053339","https://openalex.org/W1996497126","https://openalex.org/W1998223524","https://openalex.org/W2000101394","https://openalex.org/W2002783994","https://openalex.org/W2008037342","https://openalex.org/W2011853969","https://openalex.org/W2013504292","https://openalex.org/W2016150537","https://openalex.org/W2016699164","https://openalex.org/W2017953073","https://openalex.org/W2018097826","https://openalex.org/W2019477145","https://openalex.org/W2023401082","https://openalex.org/W2024661515","https://openalex.org/W2025348942","https://openalex.org/W2030045789","https://openalex.org/W2031521660","https://openalex.org/W2031597564","https://openalex.org/W2036670909","https://openalex.org/W2037180748","https://openalex.org/W2040773265","https://openalex.org/W2040820914","https://openalex.org/W2041986497","https://openalex.org/W2042909802","https://openalex.org/W2044397978","https://openalex.org/W2044827162","https://openalex.org/W2045365669","https://openalex.org/W2047180894","https://openalex.org/W2047767613","https://openalex.org/W2047855859","https://openalex.org/W2048367533","https://openalex.org/W2049325362","https://openalex.org/W2050529746","https://openalex.org/W2051514038","https://openalex.org/W2052227382","https://openalex.org/W2052674146","https://openalex.org/W2056798726","https://openalex.org/W2059340308","https://openalex.org/W2059623464","https://openalex.org/W2063110228","https://openalex.org/W2063247265","https://openalex.org/W2064520441","https://openalex.org/W2065758637","https://openalex.org/W2066644227","https://openalex.org/W2067188612","https://openalex.org/W2067460968","https://openalex.org/W2068554546","https://openalex.org/W2068629141","https://openalex.org/W2068764558","https://openalex.org/W2068823091","https://openalex.org/W2069564134","https://openalex.org/W2071284470","https://openalex.org/W2072045976","https://openalex.org/W2074361517","https://openalex.org/W2075645013","https://openalex.org/W2076356139","https://openalex.org/W2078330627","https://openalex.org/W2081989366","https://openalex.org/W2082075384","https://openalex.org/W2082539950","https://openalex.org/W2084156003","https://openalex.org/W2085145054","https://openalex.org/W2086658389","https://openalex.org/W2087497166","https://openalex.org/W2089140170","https://openalex.org/W2089395393","https://openalex.org/W2090563259","https://openalex.org/W2091116836","https://openalex.org/W2091345117","https://openalex.org/W2091475341","https://openalex.org/W2093266344","https://openalex.org/W2099371414","https://openalex.org/W2104428993","https://openalex.org/W2105844708","https://openalex.org/W2108102256","https://openalex.org/W2108214240","https://openalex.org/W2109769822","https://openalex.org/W2111367456","https://openalex.org/W2116630730","https://openalex.org/W2125355407","https://openalex.org/W2135396216","https://openalex.org/W2139432060","https://openalex.org/W2141083396","https://openalex.org/W2141183114","https://openalex.org/W2144836511","https://openalex.org/W2146810221","https://openalex.org/W2150662486","https://openalex.org/W2152565601","https://openalex.org/W2168362482","https://openalex.org/W2168854413","https://openalex.org/W2169425069","https://openalex.org/W2174184943","https://openalex.org/W2200120976","https://openalex.org/W2216501506","https://openalex.org/W2227470433","https://openalex.org/W2247149472","https://openalex.org/W2261330103","https://openalex.org/W2316532201","https://openalex.org/W2326424320","https://openalex.org/W2331630900","https://openalex.org/W2344419589","https://openalex.org/W2397059378","https://openalex.org/W2403343829","https://openalex.org/W2409080721","https://openalex.org/W2412111055","https://openalex.org/W2414175657","https://openalex.org/W2415014782","https://openalex.org/W2419151069","https://openalex.org/W2419160648","https://openalex.org/W2419309763","https://openalex.org/W2424673444","https://openalex.org/W2491004180","https://openalex.org/W2499954806","https://openalex.org/W2503069172","https://openalex.org/W2743361728","https://openalex.org/W2771040262","https://openalex.org/W2897309473","https://openalex.org/W2911519741","https://openalex.org/W4299362479"],"related_works":["https://openalex.org/W46539579","https://openalex.org/W2433116393","https://openalex.org/W2042699855","https://openalex.org/W1983796700","https://openalex.org/W2058444351","https://openalex.org/W1997904672","https://openalex.org/W2403209435","https://openalex.org/W186151347","https://openalex.org/W4317423845","https://openalex.org/W2105844708"],"abstract_inverted_index":{"Recently,":[0],"there":[1],"have":[2,82],"been":[3],"significant":[4],"advances":[5],"toward":[6],"the":[7,25,33,37,40,47,99,103,109,114,130,134,143],"development":[8],"of":[9,15,27,35,42,53,138],"culture":[10,66,131],"conditions":[11,132],"that":[12],"promote":[13],"proliferation":[14,135],"primary":[16,59,139],"rodent":[17],"hepatocytes.":[18],"There":[19],"are":[20,72],"two":[21],"major":[22],"methods":[23],"for":[24,121,133],"multiplication":[26],"hepatocytes":[28,60,81,111,140],"in":[29,149],"vitro:":[30],"one":[31],"is":[32,39],"use":[34,41],"nicotinamide,":[36],"other":[38,100],"a":[43,50,56,83,122],"nutrient-rich":[44,104],"medium.":[45],"In":[46,64,125],"medium":[48,105],"containing":[49],"high":[51,84],"concentration":[52],"nicotinamide":[54],"and":[55,77,92,118,136,141],"growth":[57],"factor,":[58],"can":[61,93,112],"proliferate":[62,87],"well.":[63],"this":[65,126],"condition":[67],"small":[68,74,144],"mononucleate":[69],"cells,":[70],"which":[71],"named":[73],"hepatocytes,":[75,145],"appear":[76],"form":[78],"colonies.":[79],"Small":[80],"potential":[85],"to":[86],"while":[88],"maintaining":[89],"hepatic":[90,115],"characteristics,":[91],"differentiate":[94],"into":[95],"mature":[96],"ones.":[97],"On":[98],"hand,":[101],"combining":[102],"with":[106],"2%":[107],"DMSO,":[108],"proliferated":[110],"recover":[113],"differentiated":[116],"functions":[117],"maintain":[119],"them":[120],"long":[123],"time.":[124],"review":[127],"I":[128],"describe":[129],"differentiation":[137],"discuss":[142],"especially":[146],"their":[147],"roles":[148],"liver":[150],"growth.":[151]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}