{"id":"https://openalex.org/W1895042658","doi":"https://doi.org/10.1242/dev.104.2.205","title":"Tissue effects on the expression of serotonin, tyrosine hydroxylase and GABA in cultures of neurogenic cells from the neuraxis and branchial arches","display_name":"Tissue effects on the expression of serotonin, tyrosine hydroxylase and GABA in cultures of neurogenic cells from the neuraxis and branchial arches","publication_year":1988,"publication_date":"1988-10-01","ids":{"openalex":"https://openalex.org/W1895042658","doi":"https://doi.org/10.1242/dev.104.2.205","mag":"1895042658","pmid":"https://pubmed.ncbi.nlm.nih.gov/2908201"},"language":"en","primary_location":{"id":"doi:10.1242/dev.104.2.205","doi":"https://doi.org/10.1242/dev.104.2.205","is_oa":false,"landing_page_url":"https://doi.org/10.1242/dev.104.2.205","pdf_url":null,"source":{"id":"https://openalex.org/S86558740","display_name":"Development","issn_l":"0950-1991","issn":["0950-1991","1477-9129"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311847","host_organization_name":"The Company of Biologists","host_organization_lineage":["https://openalex.org/P4310311847"],"host_organization_lineage_names":["The Company of Biologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Development","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113514224","display_name":"Harris M. Mackey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Harris M. Mackey","raw_affiliation_strings":["College of Physicians and Surgeons of Columbia University Department of Anatomy and Cell Biology , , 630 West 168th Street, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Physicians and Surgeons of Columbia University Department of Anatomy and Cell Biology , , 630 West 168th Street, New York, NY 10032, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030839138","display_name":"Robert F. Payette","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert F. Payette","raw_affiliation_strings":["College of Physicians and Surgeons of Columbia University Department of Anatomy and Cell Biology , , 630 West 168th Street, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Physicians and Surgeons of Columbia University Department of Anatomy and Cell Biology , , 630 West 168th Street, New York, NY 10032, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019329770","display_name":"Michael David Gershon","orcid":"https://orcid.org/0000-0002-5893-7643","observed_orcids":["https://orcid.org/0000-0002-5893-7643"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael D. Gershon","raw_affiliation_strings":["College of Physicians and Surgeons of Columbia University Department of Anatomy and Cell Biology , , 630 West 168th Street, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Physicians and Surgeons of Columbia University Department of Anatomy and Cell Biology , , 630 West 168th Street, New York, NY 10032, USA","institution_ids":["https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I78577930"],"apc_list":null,"apc_paid":null,"fwci":5.1748,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.9481952,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"104","issue":"2","first_page":"205","last_page":"217"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12237","display_name":"Congenital gastrointestinal and neural anomalies","score":0.7949000000953674,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12237","display_name":"Congenital gastrointestinal and neural anomalies","score":0.7949000000953674,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0737999975681305,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.06970000267028809,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enteric-nervous-system","display_name":"enteric nervous system","score":0.8970000147819519},{"id":"https://openalex.org/keywords/neural-crest","display_name":"neural crest","score":0.7310000061988831},{"id":"https://openalex.org/keywords/serotonin","display_name":"serotonin","score":0.5740000009536743},{"id":"https://openalex.org/keywords/tyrosine-hydroxylase","display_name":"tyrosine hydroxylase","score":0.9649999737739563},{"id":"https://openalex.org/keywords/gaba","display_name":"GABA","score":0.9909999966621399},{"id":"https://openalex.org/keywords/neuronal-differentiation","display_name":"neuronal differentiation","score":0.33500000834465027},{"id":"https://openalex.org/keywords/neuraxis","display_name":"neuraxis","score":0.4959999918937683},{"id":"https://openalex.org/keywords/pharyngeal-arches","display_name":"branchial arches","score":0.9929999709129333},{"id":"https://openalex.org/keywords/nerve-growth-factor","display_name":"nerve growth factor","score":0.7710000276565552},{"id":"https://openalex.org/keywords/co-culture","display_name":"co-culture","score":0.5260000228881836},{"id":"https://openalex.org/keywords/chick-embryo","display_name":"chick embryo","score":0.7689999938011169},{"id":"https://openalex.org/keywords/phenotypic-suppression","display_name":"phenotypic suppression","score":0.6179999709129333},{"id":"https://openalex.org/keywords/avian-intestine","display_name":"avian intestine","score":0.5450000166893005},{"id":"https://openalex.org/keywords/hindgut","display_name":"hindgut","score":0.42100000381469727}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9160956144332886},{"id":"https://openalex.org/C159167319","wikidata":"https://www.wikidata.org/wiki/Q420766","display_name":"Tyrosine hydroxylase","level":3,"score":0.7867302298545837},{"id":"https://openalex.org/C2775864247","wikidata":"https://www.wikidata.org/wiki/Q167934","display_name":"Serotonin","level":3,"score":0.6794485449790955},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.49767258763313293},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.45996102690696716},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.45738524198532104},{"id":"https://openalex.org/C2778094651","wikidata":"https://www.wikidata.org/wiki/Q3621760","display_name":"Branchial arch","level":3,"score":0.44828617572784424},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4378418028354645},{"id":"https://openalex.org/C2775951788","wikidata":"https://www.wikidata.org/wiki/Q418293","display_name":"Tryptophan hydroxylase","level":5,"score":0.4350675940513611},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3694270849227905},{"id":"https://openalex.org/C37000724","wikidata":"https://www.wikidata.org/wiki/Q50430226","display_name":"Serotonergic","level":4,"score":0.3383074402809143},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.153262197971344},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13647383451461792},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.12839168310165405},{"id":"https://openalex.org/C513476851","wikidata":"https://www.wikidata.org/wiki/Q170304","display_name":"Dopamine","level":2,"score":0.12382903695106506},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001934","descriptor_name":"Branchial Region","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002642","descriptor_name":"Chick Embryo","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005680","descriptor_name":"gamma-Aminobutyric Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007422","descriptor_name":"Intestines","qualifier_ui":"Q000294","qualifier_name":"innervation","is_major_topic":true},{"descriptor_ui":"D009432","descriptor_name":"Neural Crest","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011784","descriptor_name":"Quail","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012701","descriptor_name":"Serotonin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014446","descriptor_name":"Tyrosine 3-Monooxygenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1242/dev.104.2.205","doi":"https://doi.org/10.1242/dev.104.2.205","is_oa":false,"landing_page_url":"https://doi.org/10.1242/dev.104.2.205","pdf_url":null,"source":{"id":"https://openalex.org/S86558740","display_name":"Development","issn_l":"0950-1991","issn":["0950-1991","1477-9129"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311847","host_organization_name":"The Company of Biologists","host_organization_lineage":["https://openalex.org/P4310311847"],"host_organization_lineage_names":["The Company of Biologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Development","raw_type":"journal-article"},{"id":"pmid:2908201","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2908201","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Development (Cambridge, England)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2401284123","display_name":null,"funder_award_id":"NS 15547","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G3460354724","display_name":"CONGENITAL MEGACOLON: TISSUE INTERACTIONS IN DEVELOPMENT","funder_award_id":"R01HD017736","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"funders":[{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":50,"referenced_works":["https://openalex.org/W1491527820","https://openalex.org/W1497427077","https://openalex.org/W1505379989","https://openalex.org/W1508582984","https://openalex.org/W1520420711","https://openalex.org/W1565566882","https://openalex.org/W1671477864","https://openalex.org/W1963871661","https://openalex.org/W1966742876","https://openalex.org/W1979647423","https://openalex.org/W1985268058","https://openalex.org/W1985467463","https://openalex.org/W1989847792","https://openalex.org/W1990123221","https://openalex.org/W1991197291","https://openalex.org/W1996306346","https://openalex.org/W2000159936","https://openalex.org/W2000619428","https://openalex.org/W2004561489","https://openalex.org/W2006780982","https://openalex.org/W2008681849","https://openalex.org/W2010091519","https://openalex.org/W2011001942","https://openalex.org/W2017653616","https://openalex.org/W2021429658","https://openalex.org/W2025134970","https://openalex.org/W2027966767","https://openalex.org/W2031694081","https://openalex.org/W2041346939","https://openalex.org/W2041566213","https://openalex.org/W2043418794","https://openalex.org/W2049874270","https://openalex.org/W2051376619","https://openalex.org/W2058656221","https://openalex.org/W2086227547","https://openalex.org/W2086543710","https://openalex.org/W2088078672","https://openalex.org/W2089348287","https://openalex.org/W2089855907","https://openalex.org/W2089896043","https://openalex.org/W2096509337","https://openalex.org/W2133023377","https://openalex.org/W2140034535","https://openalex.org/W2158385323","https://openalex.org/W2161045170","https://openalex.org/W2181892845","https://openalex.org/W2296858391","https://openalex.org/W2345783831","https://openalex.org/W2394544256","https://openalex.org/W2410574573"],"related_works":["https://openalex.org/W2011001942","https://openalex.org/W2185492648","https://openalex.org/W2089855907","https://openalex.org/W2021429658","https://openalex.org/W1979185250","https://openalex.org/W2000619428","https://openalex.org/W1491527820","https://openalex.org/W2166928208","https://openalex.org/W2021923636","https://openalex.org/W2091455331"],"abstract_inverted_index":{"The":[0],"phenotypically":[1],"diverse":[2],"neurones":[3,59],"of":[4,25,36,44,83,142,158,180,202,225,236,238,248,280,336],"the":[5,17,20,26,34,42,54,91,233,241,246,256,261,281,284,295,311,324,334,337],"enteric":[6,37],"nervous":[7],"system":[8],"are":[9,253,275,298],"developmentally":[10],"derived":[11],"from":[12,19,182],"precursors":[13,259],"that":[14,118,150,157,258,268],"migrate":[15],"to":[16,30,240,263,266,286],"bowel":[18,203],"vagal":[21,85],"and":[22,49,101,107,146,196,272,303,331],"sacral":[23],"regions":[24],"neuraxis.":[27],"In":[28,53],"order":[29],"gain":[31],"insight":[32],"into":[33],"generation":[35],"neuronal":[38],"diversity,":[39],"we":[40],"examined":[41],"expression":[43,247,305],"serotonin":[45],"(5-HT),":[46],"tyrosine":[47,66,111,152,190,211,273,321],"hydroxylase":[48,126,153,191,212,274,322],"GABA":[50,63,197,271,304],"in":[51,81,95,116,121,315,323],"vitro.":[52],"mature":[55],"avian":[56],"intestine,":[57],"intrinsic":[58],"contain":[60],"5-HT":[61,106,163,195,249,302],"or":[62,74,86,160,208,222,314],"but":[64,109,155],"not":[65,110,156],"hydroxylase.":[67],"These":[68,251],"markers":[69,79],"were":[70,93,119,170,217,230],"demonstrated":[71],"immunocytochemically,":[72],"singly":[73],"simultaneously.":[75],"All":[76],"three":[77],"phenotypic":[78],"developed":[80,115],"cultures":[82,92,117,201],"cranial,":[84],"truncal":[87],"neural":[88],"crest":[89,296,307],"when":[90,130,167],"grown":[94,120],"enriched":[96],"medium,":[97],"containing":[98],"horse":[99],"serum":[100],"chick":[102,177,183],"embryo":[103],"extract;":[104],"however,":[105,162,199,283],"GABA,":[108],"hydroxylase-immunoreactive":[112],"cells,":[113],"also":[114],"partially":[122,131],"defined":[123,132],"medium.":[124],"Tyrosine":[125],"immunoreactivity":[127,164],"was":[128,134,165],"seen":[129,166],"medium":[133],"supplemented":[135],"with":[136,172,219,255,260],"nerve":[137],"growth":[138],"factor":[139],"(NGF).":[140],"Cultures":[141,179],"branchial":[143,168,220,312],"arches":[144,169,221],"(III":[145],"IV)":[147],"contained":[148,189],"cells":[149,181,216,229,267,297,308],"displayed":[151],"immunoreactivity,":[154],"5-HT-":[159],"GABA-;":[161],"cocultured":[171,218],"aneuronal":[173],"hindgut":[174],"(from":[175],"4-day":[176],"embryos).":[178],"gut":[184,239],"dissociated":[185,204],"at":[186,205,277],"7":[187],"days":[188,207],"as":[192,194],"well":[193],"immunoreactivities;":[198],"no":[200,227],"8":[206],"later":[209],"expressed":[210],"immunoreactivity.":[213,250],"When":[214],"neuraxial":[215],"heart":[223],"instead":[224],"gut,":[226],"5-HT-immunoreactive":[228],"seen;":[231],"nevertheless,":[232],"further":[234],"addition":[235],"explants":[237],"heart/crest":[242],"cocultures":[243],"did":[244],"permit":[245],"results":[252],"consistent":[254],"hypotheses":[257],"potential":[262],"give":[264],"rise":[265],"express":[269,287],"5-HT,":[270],"found":[276],"several":[278],"levels":[279],"neuraxis;":[282],"ability":[285],"these":[288],"phenotypes":[289],"may":[290,328],"be":[291,329],"suppressed":[292],"either":[293],"while":[294],"migrating":[299],"(for":[300,319],"example,":[301,320],"by":[306,333],"passing":[309],"through":[310],"arches)":[313],"their":[316],"final":[317],"destination":[318],"gut).":[325],"This":[326],"suppression":[327],"transient":[330],"reversed":[332],"microenvironment":[335],"target":[338],"organs.":[339]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}