{"id":"https://openalex.org/W189125649","doi":null,"title":"HER2/neu expression and gene alterations in pancreatic ductal adenocarcinoma: a comparative immunohistochemistry and chromogenic in situ hybridization study based on tissue microarrays and computerized image analysis.","display_name":"HER2/neu expression and gene alterations in pancreatic ductal adenocarcinoma: a comparative immunohistochemistry and chromogenic in situ hybridization study based on tissue microarrays and computerized image analysis.","publication_year":2006,"publication_date":"2006-05-09","ids":{"openalex":"https://openalex.org/W189125649","mag":"189125649","pmid":"https://pubmed.ncbi.nlm.nih.gov/16685109"},"language":"en","primary_location":{"id":"pmid:16685109","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16685109","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JOP : Journal of the pancreas","raw_type":"Comparative Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085110763","display_name":"Evangelos Tsiambas","orcid":"https://orcid.org/0000-0001-5541-2465","observed_orcids":["https://orcid.org/0000-0001-5541-2465"]},"institutions":[{"id":"https://openalex.org/I4210106119","display_name":"NIMTS Hospital","ror":"https://ror.org/00y1kmp77","country_code":"GR","type":"healthcare","lineage":["https://openalex.org/I4210106119"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"Evangelos Tsiambas","raw_affiliation_strings":["Department of Pathology, 417 VA Hospital (NIMTS), Athens, Greece. tsiambasecyto@yahoo.gr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, 417 VA Hospital (NIMTS), Athens, Greece. tsiambasecyto@yahoo.gr","institution_ids":["https://openalex.org/I4210106119"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000349587","display_name":"Andreas Karameris","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Andreas Karameris","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108218933","display_name":"Christos Dervenis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Christos Dervenis","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007937371","display_name":"Andreas Ch Lazaris","orcid":"https://orcid.org/0000-0002-8118-7780","observed_orcids":["https://orcid.org/0000-0002-8118-7780"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Andreas C Lazaris","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109397037","display_name":"Niki Giannakou","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Niki Giannakou","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031343112","display_name":"Kyriakos Gerontopoulos","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kyriakos Gerontopoulos","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5113636651","display_name":"Efstratios Patsouris","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Efstratios Patsouris","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.4315,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.87905172,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"7","issue":"3","first_page":"283","last_page":"94"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.5346999764442444,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.5346999764442444,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10231","display_name":"Pancreatic and Hepatic Oncology Research","score":0.4359999895095825,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10696","display_name":"Gastric Cancer Management and Outcomes","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/her2-neu","display_name":"HER2/neu","score":0.9210000038146973},{"id":"https://openalex.org/keywords/pancreatic-ductal-adenocarcinoma","display_name":"pancreatic ductal adenocarcinoma","score":0.9359999895095825},{"id":"https://openalex.org/keywords/her2-amplification","display_name":"HER2 amplification","score":0.9039999842643738},{"id":"https://openalex.org/keywords/protein-overexpression","display_name":"protein overexpression","score":0.8539999723434448},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.8299999833106995},{"id":"https://openalex.org/keywords/digital-image-analysis","display_name":"digital image analysis","score":0.8560000061988831},{"id":"https://openalex.org/keywords/interobserver-agreement","display_name":"interobserver agreement","score":0.9419999718666077},{"id":"https://openalex.org/keywords/tumor-grade","display_name":"tumor grade","score":0.9150000214576721},{"id":"https://openalex.org/keywords/targeted-therapy","display_name":"targeted therapy","score":0.5929999947547913},{"id":"https://openalex.org/keywords/anti-her2-monoclonal-antibody","display_name":"anti-HER2 monoclonal antibody","score":0.8209999799728394},{"id":"https://openalex.org/keywords/chromogenic-in-situ-hybridization","display_name":"chromogenic in situ hybridization","score":0.777999997138977},{"id":"https://openalex.org/keywords/tissue-microarray","display_name":"tissue microarray","score":0.746999979019165},{"id":"https://openalex.org/keywords/breast-cancer-comparison","display_name":"breast cancer comparison","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C193270364","wikidata":"https://www.wikidata.org/wiki/Q17141925","display_name":"Tissue microarray","level":3,"score":0.7538291215896606},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.7288423776626587},{"id":"https://openalex.org/C2776643354","wikidata":"https://www.wikidata.org/wiki/Q5113886","display_name":"Chromogenic in situ hybridization","level":5,"score":0.678114652633667},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.586871325969696},{"id":"https://openalex.org/C2781182431","wikidata":"https://www.wikidata.org/wiki/Q356033","display_name":"Adenocarcinoma","level":3,"score":0.4886372685432434},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.47295090556144714},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.4654472768306732},{"id":"https://openalex.org/C2780210213","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic cancer","level":3,"score":0.42667779326438904},{"id":"https://openalex.org/C2780862961","wikidata":"https://www.wikidata.org/wiki/Q5311598","display_name":"Ductal carcinoma","level":4,"score":0.41760337352752686},{"id":"https://openalex.org/C530470458","wikidata":"https://www.wikidata.org/wiki/Q128581","display_name":"Breast cancer","level":3,"score":0.4076421856880188},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3917408585548401},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3445597290992737},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.33056068420410156},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2739395797252655},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1945543885231018},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.15958401560783386},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000782","descriptor_name":"Aneuploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002886","descriptor_name":"Chromosomes, Human, Pair 17","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005784","descriptor_name":"Gene Amplification","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018270","descriptor_name":"Carcinoma, Ductal, Breast","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018270","descriptor_name":"Carcinoma, Ductal, Breast","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018270","descriptor_name":"Carcinoma, Ductal, Breast","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D018719","descriptor_name":"Erb-b2 Receptor Tyrosine Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018734","descriptor_name":"Genes, erbB-2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:16685109","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16685109","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JOP : Journal of the pancreas","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.540.6679","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.540.6679","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.joplink.net/prev/200605/200605_05.pdf","raw_type":"text"},{"id":"mag:189125649","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16685109","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[{"display_name":"Observational Study","id":"https://openalex.org/study-designs/observational-study"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1586153330","https://openalex.org/W1849285658","https://openalex.org/W1890330462","https://openalex.org/W1973953149","https://openalex.org/W1978651690","https://openalex.org/W1991341116","https://openalex.org/W1992885576","https://openalex.org/W1995215272","https://openalex.org/W1997752162","https://openalex.org/W2021532283","https://openalex.org/W2026049167","https://openalex.org/W2030095488","https://openalex.org/W2030753600","https://openalex.org/W2053488663","https://openalex.org/W2060084956","https://openalex.org/W2072648072","https://openalex.org/W2076440683","https://openalex.org/W2090354070","https://openalex.org/W2093758103","https://openalex.org/W2100723992","https://openalex.org/W2108100098","https://openalex.org/W2114864270","https://openalex.org/W2114974083","https://openalex.org/W2129211249","https://openalex.org/W2136701318","https://openalex.org/W2139713460","https://openalex.org/W2157819047","https://openalex.org/W2159363679","https://openalex.org/W2160149526","https://openalex.org/W2161942325","https://openalex.org/W2164632816","https://openalex.org/W2187449372","https://openalex.org/W2333953473","https://openalex.org/W2404465863","https://openalex.org/W2417796160","https://openalex.org/W2434343465"],"related_works":["https://openalex.org/W7096927817","https://openalex.org/W2361281112","https://openalex.org/W1959036788","https://openalex.org/W2030753600","https://openalex.org/W2035758183","https://openalex.org/W2249571958","https://openalex.org/W2129211249","https://openalex.org/W1964010757","https://openalex.org/W2409035899","https://openalex.org/W2357760177"],"abstract_inverted_index":{"CONTEXT:":[0],"HER2/neu":[1,20,117,184,226,275],"overexpression":[2,161,235],"is":[3,223],"observed":[4],"in":[5,47,62,122,131,137,157,198,255,308],"many":[6],"cancers":[7],"including":[8],"pancreatic":[9,39,220,309],"ductal":[10,40,221,310],"adenocarcinoma.":[11,311],"Although":[12],"immunohistochemistry":[13],"remains":[14],"the":[15,86,93,107,146,158,248,253,261,291],"basic":[16],"method":[17],"for":[18],"evaluating":[19],"protein":[21,185,234,276],"expression,":[22],"significant":[23],"information":[24],"regarding":[25],"gene":[26,118,203,227,241],"status":[27,204],"cannot":[28],"be":[29],"assessed.":[30],"DESIGN:":[31],"Using":[32],"tissue":[33],"microarray":[34],"technology,":[35],"fifty":[36],"histologically":[37],"confirmed":[38],"adenocarcinomas":[41,222],"were":[42,66,71,89,104,175,205],"cored":[43],"twice":[44],"and":[45,55,77,85,153,171,181,202,210,283,293,305],"re-embedded":[46],"one":[48],"paraffin":[49],"block.":[50],"Immunohistochemistry":[51],"(clone":[52],"TAB":[53],"250)":[54],"chromogenic":[56],"(HER2/neu":[57],"amplification":[58,119],"Spot":[59],"Light":[60],"kit)":[61],"situ":[63],"hybridization":[64],"protocols":[65],"performed.":[67],"The":[68,81,99],"immunostained":[69],"slides":[70],"evaluated":[72],"by":[73,91,106,150,225],"conventional":[74,151,287],"eye":[75,152,288],"microscopy":[76,289],"digital":[78,154,280],"image":[79,113,281],"analysis.":[80],"chi":[82],"square":[83],"test":[84],"kappa":[87],"statistic":[88],"applied":[90],"running":[92],"SPSS":[94],"package.":[95],"MAIN":[96],"OUTCOME":[97],"MEASURES:":[98],"levels":[100],"of":[101,109,160,219,252,274,295],"staining":[102],"intensity":[103],"estimated":[105],"performance":[108],"a":[110,217],"semi":[111],"automated":[112],"analysis":[114,282],"system.":[115],"RESULTS:":[116],"was":[120,129,143,187],"detected":[121,130],"8/50":[123],"cases":[124,133,159,174],"(16%).":[125],"Chromosome":[126],"17":[127,201],"aneuploidy":[128],"19":[132],"(38%).":[134],"Significant":[135],"improvement":[136],"interobserver":[138],"agreement":[139],"(kappa=0.76":[140],"vs.":[141],"0.94)":[142],"achieved":[144],"correlating":[145],"immunohistochemical":[147,296],"results":[148,214],"obtained":[149],"microscopy,":[155],"especially":[156],"(2+,":[162],"3+).":[163],"Finally,":[164],"29":[165],"(58%),":[166],"11":[167],"(22%),":[168],"6":[169],"(12%)":[170],"4":[172],"(8%)":[173],"characterized":[176,224],"as":[177,268],"0,":[178],"1+,":[179],"2+":[180],"3+,":[182],"respectively.":[183],"expression":[186,277],"significantly":[188],"associated":[189],"with":[190,195,208],"grade":[191],"(P=0.019),":[192],"but":[193],"not":[194,206,237,284,301],"stage":[196,209],"(P=0.466).":[197],"addition,":[199],"chromosome":[200],"correlated":[207],"grade.":[211],"CONCLUSION:":[212],"Our":[213],"indicate":[215],"that":[216,299],"subset":[218],"amplification.":[228],"In":[229],"contrast":[230],"to":[231,263],"breast":[232],"cancer,":[233],"does":[236,300],"predict":[238],"this":[239],"specific":[240],"deregulation":[242],"mechanism.":[243],"This":[244],"event":[245],"may":[246],"reflect":[247],"different":[249],"biological":[250],"role":[251],"molecule":[254],"those":[256],"two":[257],"solid":[258],"tumours,":[259],"affecting":[260],"response":[262],"novel":[264],"targeted":[265],"agents,":[266],"such":[267],"monoclonal":[269],"anti-HER2/neu":[270],"antibodies.":[271],"Furthermore,":[272],"evaluation":[273],"based":[278],"on":[279,286],"only":[285],"improves":[290],"accuracy":[292],"reliability":[294],"estimation,":[297],"although":[298],"demonstrate":[302],"clinical":[303],"significance":[304],"prognostic":[306],"value":[307]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}