{"id":"https://openalex.org/W189048726","doi":"https://doi.org/10.1007/978-1-62703-426-5_7","title":"A Novel Approach to Quantify G-Protein-Coupled Receptor Dimerization Equilibrium Using Bioluminescence Resonance Energy Transfer","display_name":"A Novel Approach to Quantify G-Protein-Coupled Receptor Dimerization Equilibrium Using Bioluminescence Resonance Energy Transfer","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W189048726","doi":"https://doi.org/10.1007/978-1-62703-426-5_7","mag":"189048726","pmid":"https://pubmed.ncbi.nlm.nih.gov/23625495","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4091634"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-62703-426-5_7","doi":"https://doi.org/10.1007/978-1-62703-426-5_7","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-426-5_7","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4244880158","display_name":"Chemokines"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4091634","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004550798","display_name":"Irina Kufareva","orcid":"https://orcid.org/0000-0001-9083-7039","observed_orcids":["https://orcid.org/0000-0001-9083-7039"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Irina Kufareva","raw_affiliation_strings":["Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030326848","display_name":"Bryan S. Stephens","orcid":"https://orcid.org/0000-0002-5398-7406","observed_orcids":["https://orcid.org/0000-0002-5398-7406"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bryan Stephens","raw_affiliation_strings":["Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113387797","display_name":"Christian Taylor Gilliland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Taylor Gilliland","raw_affiliation_strings":["Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062705887","display_name":"Beili Wu","orcid":"https://orcid.org/0000-0002-1936-0909","observed_orcids":["https://orcid.org/0000-0002-1936-0909"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]},{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210112820","display_name":"Shanghai Institute of Materia Medica","ror":"https://ror.org/022syn853","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210112820"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Beili Wu","raw_affiliation_strings":["Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China","The Scripps Research Institute, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210112820"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064748016","display_name":"Gustavo Fenalti","orcid":"https://orcid.org/0000-0001-6554-2351","observed_orcids":["https://orcid.org/0000-0001-6554-2351"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gustavo Fenalti","raw_affiliation_strings":["The Scripps Research Institute, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040423604","display_name":"Damon J. Hamel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Damon Hamel","raw_affiliation_strings":["Janssen Research and Development, San Diego, CA, USA","Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Janssen Research and Development, San Diego, CA, USA","institution_ids":[]},{"raw_affiliation_string":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017648766","display_name":"Raymond C. Stevens","orcid":"https://orcid.org/0000-0002-4522-8725","observed_orcids":["https://orcid.org/0000-0002-4522-8725"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Raymond C. Stevens","raw_affiliation_strings":["The Scripps Research Institute, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064101943","display_name":"Ruben A Abagyan","orcid":"https://orcid.org/0000-0001-9309-2976","observed_orcids":["https://orcid.org/0000-0001-9309-2976"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruben Abagyan","raw_affiliation_strings":["Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050290780","display_name":"Tracy M. Handel","orcid":"https://orcid.org/0000-0002-2558-6138","observed_orcids":["https://orcid.org/0000-0002-2558-6138"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tracy M. Handel","raw_affiliation_strings":["Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA","institution_ids":["https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.1842,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.8763911,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"1013","issue":null,"first_page":"93","last_page":"127"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.5468999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.5468999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.4284999966621399,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.9599999785423279},{"id":"https://openalex.org/keywords/receptor-dimerization","display_name":"receptor dimerization","score":0.9539999961853027},{"id":"https://openalex.org/keywords/homodimerization","display_name":"homodimerization","score":0.765999972820282},{"id":"https://openalex.org/keywords/heterodimerization","display_name":"heterodimerization","score":0.9990000128746033},{"id":"https://openalex.org/keywords/chemokine-receptors","display_name":"chemokine receptors","score":0.8600000143051147},{"id":"https://openalex.org/keywords/cxcr4","display_name":"CXCR4","score":0.9990000128746033},{"id":"https://openalex.org/keywords/bioluminescence-resonance-energy-transfer","display_name":"bioluminescence resonance energy transfer","score":0.9559999704360962},{"id":"https://openalex.org/keywords/receptor-expression-levels","display_name":"receptor expression levels","score":0.9549999833106995},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.8550000190734863}],"concepts":[{"id":"https://openalex.org/C5176359","wikidata":"https://www.wikidata.org/wiki/Q179924","display_name":"Bioluminescence","level":2,"score":0.9133360385894775},{"id":"https://openalex.org/C106447425","wikidata":"https://www.wikidata.org/wiki/Q11379","display_name":"Energy transfer","level":2,"score":0.6052869558334351},{"id":"https://openalex.org/C139210041","wikidata":"https://www.wikidata.org/wiki/Q2145840","display_name":"Resonance (particle physics)","level":2,"score":0.5309455394744873},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.5300999283790588},{"id":"https://openalex.org/C91845479","wikidata":"https://www.wikidata.org/wiki/Q4118065","display_name":"Resonant inductive coupling","level":3,"score":0.5030116438865662},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4335673451423645},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.41728493571281433},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.3100956678390503},{"id":"https://openalex.org/C159467904","wikidata":"https://www.wikidata.org/wiki/Q2001702","display_name":"Chemical physics","level":1,"score":0.23540747165679932},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22651568055152893},{"id":"https://openalex.org/C184779094","wikidata":"https://www.wikidata.org/wiki/Q26383","display_name":"Atomic physics","level":1,"score":0.17698130011558533},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12360301613807678},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.07113054394721985},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.059491097927093506}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019718","descriptor_name":"Receptors, CXCR4","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019718","descriptor_name":"Receptors, CXCR4","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019718","descriptor_name":"Receptors, CXCR4","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D043562","descriptor_name":"Receptors, G-Protein-Coupled","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D043562","descriptor_name":"Receptors, G-Protein-Coupled","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D043562","descriptor_name":"Receptors, G-Protein-Coupled","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D055503","descriptor_name":"Protein Multimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060807","descriptor_name":"Bioluminescence Resonance Energy Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1007/978-1-62703-426-5_7","doi":"https://doi.org/10.1007/978-1-62703-426-5_7","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-426-5_7","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:23625495","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23625495","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4091634","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4091634","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:4091634","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4091634","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Affordable and clean energy","id":"https://metadata.un.org/sdg/7","score":0.8799999952316284}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1567938228","display_name":"Nanodisc Reconstitution and Characterization of Chemokine Receptors","funder_award_id":"F32GM083463","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G4277153620","display_name":null,"funder_award_id":"R01 AL100604","funder_id":"https://openalex.org/F4320332505","funder_display_name":"U.S. Public Health Service"},{"id":"https://openalex.org/G5471768970","display_name":"Graduate Training in Cellular and Molecular Pharmacology","funder_award_id":"T32GM007752","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5993825863","display_name":"Flexible Macromolecular Docking","funder_award_id":"R01GM071872","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6301329862","display_name":"Engineering Chemokine Receptor Signaling Complexes In Vitro","funder_award_id":"R01GM081763","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7016751746","display_name":"Structure, Dynamics and Activation Mechanisms of Chemokine Receptors","funder_award_id":"U01GM094612","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7026519940","display_name":"GPCR Network","funder_award_id":"U54GM094618","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8976673989","display_name":"MOLECULAR BIOPHYSICS TRAINING PROGRAM","funder_award_id":"T32GM008326","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":65,"referenced_works":["https://openalex.org/W38875437","https://openalex.org/W60873507","https://openalex.org/W1543220384","https://openalex.org/W1593172464","https://openalex.org/W1958433286","https://openalex.org/W1966815718","https://openalex.org/W1967738476","https://openalex.org/W1971256251","https://openalex.org/W1972905173","https://openalex.org/W1973245565","https://openalex.org/W1976136773","https://openalex.org/W1977700439","https://openalex.org/W1979194254","https://openalex.org/W1984429646","https://openalex.org/W1986308794","https://openalex.org/W1988880542","https://openalex.org/W1994113226","https://openalex.org/W1994511844","https://openalex.org/W1997773971","https://openalex.org/W2001541137","https://openalex.org/W2002567921","https://openalex.org/W2002768212","https://openalex.org/W2004424136","https://openalex.org/W2008544466","https://openalex.org/W2014055152","https://openalex.org/W2028953854","https://openalex.org/W2041138404","https://openalex.org/W2041211150","https://openalex.org/W2043246373","https://openalex.org/W2045120140","https://openalex.org/W2049619797","https://openalex.org/W2052392084","https://openalex.org/W2053153178","https://openalex.org/W2054483530","https://openalex.org/W2054923889","https://openalex.org/W2061568866","https://openalex.org/W2067813540","https://openalex.org/W2070524062","https://openalex.org/W2071129811","https://openalex.org/W2079052124","https://openalex.org/W2079694858","https://openalex.org/W2080092207","https://openalex.org/W2082991065","https://openalex.org/W2083725367","https://openalex.org/W2090630729","https://openalex.org/W2100491213","https://openalex.org/W2107871391","https://openalex.org/W2110783096","https://openalex.org/W2120365849","https://openalex.org/W2136672278","https://openalex.org/W2137241608","https://openalex.org/W2138325877","https://openalex.org/W2150757265","https://openalex.org/W2152853006","https://openalex.org/W2153776586","https://openalex.org/W2158207659","https://openalex.org/W2159913536","https://openalex.org/W2160121812","https://openalex.org/W2161758034","https://openalex.org/W2165357853","https://openalex.org/W2167070743","https://openalex.org/W2169101659","https://openalex.org/W2409230958","https://openalex.org/W2413395767","https://openalex.org/W2433403805"],"related_works":["https://openalex.org/W2036985430","https://openalex.org/W2074904272","https://openalex.org/W2409230958","https://openalex.org/W2417271624","https://openalex.org/W2107871391","https://openalex.org/W2067448578","https://openalex.org/W2913246513","https://openalex.org/W2901483441","https://openalex.org/W2978583522","https://openalex.org/W2004424136"],"abstract_inverted_index":{"Along":[0],"with":[1],"other":[2],"resonance":[3,8],"energy":[4,9],"transfer":[5,10],"techniques,":[6],"bioluminescence":[7],"(BRET)":[11],"has":[12,34],"emerged":[13],"as":[14,51],"an":[15],"important":[16,182],"method":[17],"for":[18,63,107,130,136,161,186],"demonstrating":[19,58],"protein-protein":[20],"interactions":[21,47],"in":[22,85,119,151],"cells.":[23],"In":[24,122],"the":[25,59,86,95,108,192,195,203,212],"field":[26],"of":[27,45,61,74,82,88,94,101,111,139,148,154,167,174,194,205,211],"G-protein-coupled":[28],"receptors,":[29,32],"including":[30],"chemokine":[31,213],"BRET":[33,112,140,155,179],"been":[35],"widely":[36],"used":[37],"to":[38,53,68,202],"investigate":[39],"homo-":[40],"and":[41,55,114,157,163,177,184,191,208],"heterodimerization,":[42],"a":[43,64,145,159],"feature":[44],"their":[46],"that":[48,127],"is":[49,134,197],"emerging":[50],"integral":[52],"function":[54],"regulation.":[56],"While":[57],"existence":[60],"dimers":[62],"given":[65],"receptor":[66,89,116,131,214],"proved":[67],"be":[69],"fairly":[70],"straightforward,":[71],"quantitative":[72,137,165,187],"comparisons":[73],"different":[75],"receptors":[76],"or":[77],"mutants":[78],"are":[79,189],"nontrivial":[80],"because":[81],"inevitable":[83],"variations":[84],"expression":[87,97,109,132],"constructs.":[90],"The":[91,170,181],"uncontrollable":[92],"parameters":[93],"cellular":[96],"machinery":[98],"make":[99],"amounts":[100],"transfected":[102],"DNA":[103],"extremely":[104],"poor":[105],"predictors":[106],"levels":[110,133],"donor":[113],"acceptor":[115],"constructs,":[117],"even":[118],"relative":[120],"terms.":[121],"this":[123],"chapter,":[124],"we":[125],"show":[126],"properly":[128],"accounting":[129],"critical":[135],"interpretation":[138],"data.":[141,180],"We":[142],"also":[143],"provide":[144],"comprehensive":[146],"account":[147],"expected":[149],"responses":[150],"all":[152],"types":[153],"experiments":[156],"propose":[158],"framework":[160,171],"uniform":[162],"accurate":[164],"treatment":[166,188],"these":[168],"responses.":[169],"allows":[172],"analysis":[173],"both":[175],"homodimer":[176],"heterodimer":[178],"caveats":[183],"obstacles":[185],"outlined,":[190],"utility":[193],"approach":[196],"illustrated":[198],"by":[199],"its":[200],"application":[201],"homodimerization":[204],"wild-type":[206],"(WT)":[207],"mutant":[209],"forms":[210],"CXCR4.":[215]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}