{"id":"https://openalex.org/W1885412271","doi":null,"title":"Application of PI-deconvolution to the screening of protein ligand combinatorial libraries using the yeast-two-hybrid assay","display_name":"Application of PI-deconvolution to the screening of protein ligand combinatorial libraries using the yeast-two-hybrid assay","publication_year":2008,"publication_date":"2008-11-28","ids":{"openalex":"https://openalex.org/W1885412271","mag":"1885412271"},"language":"en","primary_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-11242008-141444","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-11242008-141444","pdf_url":"http://hdl.handle.net/10388/etd-11242008-141444","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10388/etd-11242008-141444","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084681352","display_name":"Alberto Aparicio de Navaraez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Alberto Aparicio de Navaraez","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5084681352"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.21250000596046448,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.21250000596046448,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.16189999878406525,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11798","display_name":"Optimal Experimental Design Methods","score":0.1103999987244606,"subfield":{"id":"https://openalex.org/subfields/1803","display_name":"Management Science and Operations Research"},"field":{"id":"https://openalex.org/fields/18","display_name":"Decision Sciences"},"domain":{"id":"https://openalex.org/domains/2","display_name":"Social Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/yeast-two-hybrid","display_name":"yeast two-hybrid","score":0.8930000066757202},{"id":"https://openalex.org/keywords/pooling-designs","display_name":"pooling designs","score":0.6690000295639038},{"id":"https://openalex.org/keywords/false-positive-detection","display_name":"false positive detection","score":0.6800000071525574},{"id":"https://openalex.org/keywords/bcr-abl","display_name":"BCR-ABL","score":0.9350000023841858},{"id":"https://openalex.org/keywords/intein","display_name":"intein","score":0.6110000014305115},{"id":"https://openalex.org/keywords/sh2-domain","display_name":"SH2 domain","score":0.43700000643730164},{"id":"https://openalex.org/keywords/sh3-domain","display_name":"SH3 domain","score":0.9010000228881836},{"id":"https://openalex.org/keywords/kinase-inhibitors","display_name":"kinase inhibitors","score":0.5659999847412109},{"id":"https://openalex.org/keywords/chronic-myeloid-leukemia","display_name":"chronic myeloid leukemia","score":0.9490000009536743},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.6259999871253967},{"id":"https://openalex.org/keywords/combinatorial-library-screening","display_name":"combinatorial library screening","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6691670417785645},{"id":"https://openalex.org/C174576160","wikidata":"https://www.wikidata.org/wiki/Q1183700","display_name":"Deconvolution","level":2,"score":0.5263563394546509},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.45076143741607666},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.42992717027664185},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42194676399230957},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.41628581285476685},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32370656728744507},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27074432373046875},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.22732222080230713},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.10256937146186829}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ecommons.usask.ca:10388/etd-11242008-141444","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-11242008-141444","pdf_url":"http://hdl.handle.net/10388/etd-11242008-141444","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},{"id":"pmh:oai:harvest.usask.ca:10388/etd-11242008-141444","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10388/etd-11242008-141444","pdf_url":null,"source":{"id":"https://openalex.org/S7407063267","display_name":"HARVEST (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:1885412271","doi":null,"is_oa":false,"landing_page_url":"https://harvest.usask.ca/bitstream/10388/etd-11242008-141444/1/APARICIO_2008_MSc_Thesis.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-11242008-141444","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-11242008-141444","pdf_url":"http://hdl.handle.net/10388/etd-11242008-141444","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1885412271.pdf","grobid_xml":"https://content.openalex.org/works/W1885412271.grobid-xml"},"referenced_works_count":119,"referenced_works":["https://openalex.org/W127928206","https://openalex.org/W158709665","https://openalex.org/W1484834653","https://openalex.org/W1491301840","https://openalex.org/W1507805168","https://openalex.org/W1511922189","https://openalex.org/W1545752332","https://openalex.org/W1564113586","https://openalex.org/W1585894933","https://openalex.org/W1678954343","https://openalex.org/W1719330231","https://openalex.org/W1840524043","https://openalex.org/W1895993106","https://openalex.org/W1918211917","https://openalex.org/W1964602459","https://openalex.org/W1965176789","https://openalex.org/W1967024254","https://openalex.org/W1969782679","https://openalex.org/W1974798932","https://openalex.org/W1975771882","https://openalex.org/W1976704214","https://openalex.org/W1977186059","https://openalex.org/W1979454287","https://openalex.org/W1982717121","https://openalex.org/W1983860551","https://openalex.org/W1987029283","https://openalex.org/W1989187403","https://openalex.org/W1989427963","https://openalex.org/W1991198879","https://openalex.org/W1991296267","https://openalex.org/W1994652908","https://openalex.org/W1995238451","https://openalex.org/W1997140988","https://openalex.org/W1998003752","https://openalex.org/W2002503059","https://openalex.org/W2003746638","https://openalex.org/W2005997381","https://openalex.org/W2006467763","https://openalex.org/W2008295579","https://openalex.org/W2009179035","https://openalex.org/W2012536704","https://openalex.org/W2012828386","https://openalex.org/W2012879673","https://openalex.org/W2016248432","https://openalex.org/W2016656445","https://openalex.org/W2016970095","https://openalex.org/W2017047728","https://openalex.org/W2017376571","https://openalex.org/W2022228265","https://openalex.org/W2023569481","https://openalex.org/W2026879594","https://openalex.org/W2028646293","https://openalex.org/W2029242273","https://openalex.org/W2034862493","https://openalex.org/W2036158456","https://openalex.org/W2037735021","https://openalex.org/W2039504078","https://openalex.org/W2041236494","https://openalex.org/W2044154766","https://openalex.org/W2046970733","https://openalex.org/W2047498978","https://openalex.org/W2048322056","https://openalex.org/W2049743105","https://openalex.org/W2051471006","https://openalex.org/W2053912380","https://openalex.org/W2054338414","https://openalex.org/W2054592480","https://openalex.org/W2055013289","https://openalex.org/W2057147161","https://openalex.org/W2057520802","https://openalex.org/W2057684979","https://openalex.org/W2058320634","https://openalex.org/W2061913305","https://openalex.org/W2065625685","https://openalex.org/W2065630930","https://openalex.org/W2067315458","https://openalex.org/W2068339906","https://openalex.org/W2069282766","https://openalex.org/W2070758793","https://openalex.org/W2071099979","https://openalex.org/W2073517578","https://openalex.org/W2074598493","https://openalex.org/W2082580993","https://openalex.org/W2084938664","https://openalex.org/W2086889737","https://openalex.org/W2096003848","https://openalex.org/W2108796583","https://openalex.org/W2108944462","https://openalex.org/W2109516955","https://openalex.org/W2109999981","https://openalex.org/W2113654464","https://openalex.org/W2115093096","https://openalex.org/W2116531509","https://openalex.org/W2117894482","https://openalex.org/W2123812502","https://openalex.org/W2126528399","https://openalex.org/W2128341317","https://openalex.org/W2129552562","https://openalex.org/W2131174104","https://openalex.org/W2133124453","https://openalex.org/W2134962337","https://openalex.org/W2146697568","https://openalex.org/W2150053237","https://openalex.org/W2150148922","https://openalex.org/W2150891631","https://openalex.org/W2152458717","https://openalex.org/W2161835431","https://openalex.org/W2162525969","https://openalex.org/W2163753017","https://openalex.org/W2164689880","https://openalex.org/W2165701362","https://openalex.org/W2166558964","https://openalex.org/W2170272380","https://openalex.org/W2293685045","https://openalex.org/W2303097896","https://openalex.org/W2331506194","https://openalex.org/W2415539763","https://openalex.org/W2537517747","https://openalex.org/W2608773137"],"related_works":["https://openalex.org/W2141209986","https://openalex.org/W2109516955","https://openalex.org/W2384925484","https://openalex.org/W2578680839","https://openalex.org/W7146367374","https://openalex.org/W2090715543","https://openalex.org/W4312742371","https://openalex.org/W1500022004","https://openalex.org/W7030363085","https://openalex.org/W313406992"],"abstract_inverted_index":{"Reagents":[0],"that":[1,165,216,233,254,325],"bind":[2],"proteins":[3],"are":[4,30,78,101,150,320],"applicable":[5],"in":[6,80,103,169,225,248],"biology":[7],"for":[8,219,271,283,314],"detection":[9,57,181],"of":[10,13,18,58,75,123,133,162,176,182,193,200,239,251,265,280,310,323,335],"molecules,":[11],"perturbation":[12],"signaling":[14],"pathways":[15],"and":[16,172,282,295],"development":[17],"small-molecule":[19],"pharmaceuticals.Protein":[20],"ligands":[21],"interact":[22,36],"with":[23,37,197,212,276,286,291,297,338],"proteins,":[24],"inhibiting":[25],"or":[26,48],"altering":[27],"their":[28,315],"function.They":[29],"isolated":[31,317],"from":[32],"combinatorial":[33,108,126,138,195,227,336],"libraries":[34,95,127,337],"to":[35,71,92,110,117,120,178,189,206,306],"a":[38,84,170,191,194,209,237,301],"specific":[39],"target,":[40],"using":[41,96,128],"selection":[42],"techniques":[43],"such":[44],"as":[45],"phage":[46],"display":[47],"yeast-two-hybrid":[49,130,185,340],"assay.For":[50],"the":[51,56,69,81,97,111,121,129,134,137,143,159,173,180,226,252,266,277,287,292,298,308,311,339],"latter,":[52],"one":[53,290,296],"inconvenience":[54],"is":[55,208,217],"false":[59],"positives,":[60],"which":[61,100,202],"can":[62,166,326],"be":[63,72,167],"solved":[64],"by":[65],"screening":[66,113,122,192,238],"pools":[67,82],"containing":[68,136],"samples":[70],"tested,":[73],"instead":[74],"individual":[76],"samples.Samples":[77],"distributed":[79],"following":[83],"pooling":[85,88],"design.The":[86],"PI-deconvolution":[87,112,152],"design":[89],"was":[90,140,187,229,242,304],"developed":[91,116],"screen":[93],"cDNA":[94],"yeasttwo-hybrid":[98,144],"assay,":[99],"smaller":[102],"size":[104],"than":[105],"protein":[106,124,211],"ligand":[107,125],"libraries.Modifications":[109],"technique":[114],"were":[115,203,246,261,269],"adapt":[118],"it":[119],"assay.Every":[131],"spot":[132],"array":[135],"library":[139,163,196,228,241],"randomly":[141],"pooled.However,":[142],"assay":[145,186,303],"loses":[146],"sensitivity":[147],"when":[148],"strains":[149],"pooled.As":[151],"requires":[153],"detecting":[154],"every":[155],"interaction,":[156],"we":[157],"determined":[158,270],"optimal":[160,174],"amount":[161],"members":[164],"pooled":[168,204],"spot,":[171],"number":[175],"replicates":[177],"ensure":[179],"an":[183,230],"interaction.The":[184],"used":[188,224],"perform":[190],"seven":[198],"domains":[199],"BCR-ABL,":[201],"according":[205],"PI-deconvolution.BCR-ABL":[207],"chimeric":[210],"unregulated":[213],"kinase":[214],"activity":[215],"responsible":[218],"chronic":[220],"myelogenous":[221],"leukemia.The":[222],"scaffold":[223],"engineered":[231],"intein":[232],"forms":[234],"lariat":[235,267,273,312,318],"peptides.After":[236],"this":[240],"performed,":[243],"positive":[244,257],"interactions":[245],"detected":[247],"775":[249],"spots":[250,260],"arrays":[253],"contained":[255],"1432":[256],"hits.Only":[258],"53":[259],"deconvoluted.The":[262],"coding":[263],"sequences":[264],"peptides":[268,274,313,319],"23":[272],"interacted":[275],"GEF":[278],"domain":[279],"BCR,":[281],"ABL,":[284],"two":[285],"FABD":[288],"domain,":[289,294],"SH1":[293],"SH3":[299],"domain.Finally,":[300],"\u03b2-galactosidase":[302],"performed":[305],"assess":[307],"affinity":[309],"target.The":[316],"potential":[321],"inhibitors":[322],"BCR-ABL":[324],"have":[327],"therapeutic":[328],"potential.This":[329],"study":[330],"will":[331],"improve":[332],"other":[333],"screenings":[334],"assay.ORF":[341],"Open":[342],"reading":[343],"frame":[344],"PCR":[345],"Polymerase":[346],"chain":[347],"reaction":[348],"PEG":[349],"Polyethylene":[350],"glycol":[351],"ix":[352],"x":[353],"S.":[354],"cerevisiae":[355,357],"Saccharomyces":[356],"SNP":[358],"Single":[359],"nucleotide":[360],"polymorphism":[361],"STM":[362],"Human":[363],"Stefin":[364],"A":[365],"TF":[366],"Transcription":[367],"factor":[368],"-TRP":[369],"Minus":[370],"Tryptophan":[371],"X-Gal":[372],"5-bromo-4chloro-indoyl-\u03b2-D-galactopyranoside":[373],"YPDA":[374],"Yeast":[375],"peptone":[376],"dextrose":[377],"adenine":[378]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
