{"id":"https://openalex.org/W1883707147","doi":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","title":"Chemical modification of the brown\u2010fat\u2010mitochondrial uncoupling protein with tetranitromethane and N\u2010ethylmaleimide","display_name":"Chemical modification of the brown\u2010fat\u2010mitochondrial uncoupling protein with tetranitromethane and N\u2010ethylmaleimide","publication_year":1986,"publication_date":"1986-12-01","ids":{"openalex":"https://openalex.org/W1883707147","doi":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","mag":"1883707147","pmid":"https://pubmed.ncbi.nlm.nih.gov/3024980"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1986.tb10494.x","doi":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1986.tb10494.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1986.tb10494.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5062758530","display_name":"Eduardo Rial","orcid":"https://orcid.org/0000-0001-8634-8902","observed_orcids":["https://orcid.org/0000-0001-8634-8902"]},"institutions":[{"id":"https://openalex.org/I177639307","display_name":"University of Dundee","ror":"https://ror.org/03h2bxq36","country_code":"GB","type":"education","lineage":["https://openalex.org/I177639307"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Eduardo RIAL","raw_affiliation_strings":["Neurosciences Research Group, Department of Psychiatry, Ninewells Medical School, University of Dundee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Neurosciences Research Group, Department of Psychiatry, Ninewells Medical School, University of Dundee","institution_ids":["https://openalex.org/I177639307"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004270076","display_name":"David G. Nicholls","orcid":"https://orcid.org/0000-0001-5036-808X","observed_orcids":["https://orcid.org/0000-0001-5036-808X"]},"institutions":[{"id":"https://openalex.org/I177639307","display_name":"University of Dundee","ror":"https://ror.org/03h2bxq36","country_code":"GB","type":"education","lineage":["https://openalex.org/I177639307"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"David G. NICHOLLS","raw_affiliation_strings":["Neurosciences Research Group, Department of Psychiatry, Ninewells Medical School, University of Dundee"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Neurosciences Research Group, Department of Psychiatry, Ninewells Medical School, University of Dundee","institution_ids":["https://openalex.org/I177639307"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5062758530"],"corresponding_institution_ids":["https://openalex.org/I177639307"],"apc_list":null,"apc_paid":null,"fwci":4.0626,"has_fulltext":true,"cited_by_count":26,"citation_normalized_percentile":{"value":0.93669865,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"161","issue":"3","first_page":"689","last_page":"694"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11457","display_name":"Adipose Tissue and Metabolism","score":0.3361999988555908,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11457","display_name":"Adipose Tissue and Metabolism","score":0.3361999988555908,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.3197999894618988,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.20999999344348907,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brown-adipose-tissue-mitochondria","display_name":"brown adipose tissue mitochondria","score":0.8479999899864197},{"id":"https://openalex.org/keywords/uncoupling-proteins","display_name":"uncoupling proteins","score":0.8690000176429749},{"id":"https://openalex.org/keywords/tetranitromethane","display_name":"tetranitromethane","score":0.9079999923706055},{"id":"https://openalex.org/keywords/n-ethylmaleimide","display_name":"N-ethylmaleimide","score":1.0},{"id":"https://openalex.org/keywords/cysteine-modification","display_name":"cysteine modification","score":0.8820000290870667},{"id":"https://openalex.org/keywords/gdp-binding","display_name":"GDP binding","score":0.8399999737739563},{"id":"https://openalex.org/keywords/chloride-permeability","display_name":"chloride permeability","score":0.6209999918937683},{"id":"https://openalex.org/keywords/proton-permeability","display_name":"proton permeability","score":0.8479999899864197},{"id":"https://openalex.org/keywords/chemical-modification-of-proteins","display_name":"protein chemical modification","score":0.35499998927116394}],"concepts":[{"id":"https://openalex.org/C2777337673","wikidata":"https://www.wikidata.org/wiki/Q412241","display_name":"Tetranitromethane","level":3,"score":0.9929444789886475},{"id":"https://openalex.org/C2777646807","wikidata":"https://www.wikidata.org/wiki/Q292393","display_name":"N-Ethylmaleimide","level":2,"score":0.8764972686767578},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.703525960445404},{"id":"https://openalex.org/C2780642333","wikidata":"https://www.wikidata.org/wiki/Q901269","display_name":"Brown adipose tissue","level":3,"score":0.6212063431739807},{"id":"https://openalex.org/C2779406938","wikidata":"https://www.wikidata.org/wiki/Q24720289","display_name":"Uncoupling protein","level":4,"score":0.6169258952140808},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4619283080101013},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.41683048009872437},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.41520172357559204},{"id":"https://openalex.org/C171089720","wikidata":"https://www.wikidata.org/wiki/Q193583","display_name":"Adipose tissue","level":2,"score":0.22910627722740173},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.0977189838886261},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000071256","descriptor_name":"Uncoupling Protein 1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002001","descriptor_name":"Adipose Tissue, Brown","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002712","descriptor_name":"Chlorides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005033","descriptor_name":"Ethylmaleimide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D006153","descriptor_name":"Guanosine Diphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008647","descriptor_name":"Mesocricetus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008697","descriptor_name":"Methane","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010539","descriptor_name":"Permeability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D011522","descriptor_name":"Protons","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013774","descriptor_name":"Tetranitromethane","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D024101","descriptor_name":"Mitochondrial Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1986.tb10494.x","doi":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1986.tb10494.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:3024980","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3024980","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1111/j.1432-1033.1986.tb10494.x","doi":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1986.tb10494.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1986.tb10494.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334626","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1883707147.pdf","grobid_xml":"https://content.openalex.org/works/W1883707147.grobid-xml"},"referenced_works_count":33,"referenced_works":["https://openalex.org/W124504601","https://openalex.org/W125432973","https://openalex.org/W143137125","https://openalex.org/W203099801","https://openalex.org/W331117150","https://openalex.org/W1441295830","https://openalex.org/W1480789058","https://openalex.org/W1535995715","https://openalex.org/W1540415460","https://openalex.org/W1549944358","https://openalex.org/W1557140781","https://openalex.org/W1564572504","https://openalex.org/W1900866157","https://openalex.org/W1971048984","https://openalex.org/W1975323382","https://openalex.org/W1978888607","https://openalex.org/W1980455487","https://openalex.org/W1989744185","https://openalex.org/W1993452074","https://openalex.org/W2016936385","https://openalex.org/W2033259034","https://openalex.org/W2033506055","https://openalex.org/W2041416218","https://openalex.org/W2042770659","https://openalex.org/W2053994489","https://openalex.org/W2060477585","https://openalex.org/W2075332877","https://openalex.org/W2080368716","https://openalex.org/W2128796325","https://openalex.org/W2336889373","https://openalex.org/W2892750390","https://openalex.org/W2910062165","https://openalex.org/W4285701690"],"related_works":["https://openalex.org/W2060477585","https://openalex.org/W2400842204","https://openalex.org/W1534267539","https://openalex.org/W2094348039","https://openalex.org/W2087141032","https://openalex.org/W2147724871","https://openalex.org/W1980455487","https://openalex.org/W1884957018","https://openalex.org/W143137125","https://openalex.org/W1503321170"],"abstract_inverted_index":{"Treatment":[0],"of":[1,39,48,63,69,84,95],"brown":[2],"adipose":[3],"tissue":[4],"mitochondria":[5],"with":[6,13,45],"tetranitromethane":[7,90],"or":[8,60,89],"N-ethylmaleimide":[9,72,88],"decreases":[10],"the":[11,20,40,46,58,64,67,96],"affinity":[12],"which":[14,29],"inhibitory":[15],"nucleotide":[16],"GDP":[17,78],"binds":[18],"to":[19,34,98],"tissue-specific":[21],"uncoupling":[22],"protein.":[23],"Both":[24],"reagents":[25],"modify":[26],"cysteine":[27],"residues":[28],"are":[30],"'accessible'":[31],"and":[32],"'buried'":[33],"5,5'-dithio-bis(2-nitrobenzoic":[35],"acid)":[36],"(Nbs2).":[37],"Modification":[38],"single":[41],"Nbs2-accessible":[42],"residue":[43],"correlates":[44],"loss":[47],"high-affinity":[49],"binding":[50],"sites":[51],"for":[52],"GDP.":[53],"Tetranitromethane":[54],"does":[55],"not":[56],"affect":[57],"Cl-":[59,85],"H+":[61,100],"permeability":[62,86],"protein":[65],"in":[66],"absence":[68],"nucleotide,":[70],"while":[71],"increases":[73],"both":[74],"by":[75],"70-80%.":[76],"Bound":[77],"is":[79],"a":[80],"less":[81],"effective":[82],"inhibitor":[83],"after":[87],"treatment,":[91],"but":[92],"retains":[93],"much":[94],"ability":[97],"inhibit":[99],"permeation.":[101]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}