{"id":"https://openalex.org/W187733564","doi":null,"title":"The molecular mechanism of chronic delta-opioid-mediated adenylyl cyclase superactivation in Chinese hamster ovary cells stably expressing the delta-opiod receptor: A cellular model for tolerance and withdrawal","display_name":"The molecular mechanism of chronic delta-opioid-mediated adenylyl cyclase superactivation in Chinese hamster ovary cells stably expressing the delta-opiod receptor: A cellular model for tolerance and withdrawal","publication_year":2002,"publication_date":"2002-01-01","ids":{"openalex":"https://openalex.org/W187733564","mag":"187733564"},"language":"en","primary_location":{"id":"pmh:oai:repository.arizona.edu:10150/289820","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/289820","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation-Reproduction (electronic)"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061688911","display_name":"Marc K. Rubenzik","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Marc Rubenzik","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5061688911"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.9765999913215637,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.9765999913215637,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.021400000900030136,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/delta-opioid-receptor","display_name":"delta opioid receptor","score":0.9950000047683716},{"id":"https://openalex.org/keywords/drug-tolerance","display_name":"drug tolerance","score":0.7799999713897705},{"id":"https://openalex.org/keywords/opioid-withdrawal","display_name":"opioid withdrawal","score":0.996999979019165},{"id":"https://openalex.org/keywords/g-protein-beta-gamma-subunits","display_name":"G protein beta gamma subunits","score":0.8109999895095825},{"id":"https://openalex.org/keywords/phosducin","display_name":"phosducin","score":0.9900000095367432},{"id":"https://openalex.org/keywords/calmodulin","display_name":"calmodulin","score":0.9620000123977661},{"id":"https://openalex.org/keywords/atypical-protein-kinase-c","display_name":"atypical protein kinase C","score":0.9340000152587891},{"id":"https://openalex.org/keywords/raf-1","display_name":"Raf-1","score":0.9660000205039978},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.4390000104904175}],"concepts":[{"id":"https://openalex.org/C2779178603","wikidata":"https://www.wikidata.org/wiki/Q356257","display_name":"Adenylyl cyclase","level":3,"score":0.8909050822257996},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.7271738052368164},{"id":"https://openalex.org/C2778938600","wikidata":"https://www.wikidata.org/wiki/Q389934","display_name":"Agonist","level":3,"score":0.5987354516983032},{"id":"https://openalex.org/C2781063702","wikidata":"https://www.wikidata.org/wiki/Q427523","display_name":"Opioid","level":3,"score":0.5171153545379639},{"id":"https://openalex.org/C2779886867","wikidata":"https://www.wikidata.org/wiki/Q421797","display_name":"Opioid receptor","level":4,"score":0.5073310732841492},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4898676574230194},{"id":"https://openalex.org/C115466790","wikidata":"https://www.wikidata.org/wiki/Q5008667","display_name":"cAMP-dependent pathway","level":4,"score":0.48372581601142883},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.47587481141090393},{"id":"https://openalex.org/C14397066","wikidata":"https://www.wikidata.org/wiki/Q14911924","display_name":"\u03bc-opioid receptor","level":4,"score":0.47304195165634155},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4606077969074249},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.44333916902542114},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.43971139192581177},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34903356432914734},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.29623058438301086},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.18011847138404846}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repository.arizona.edu:10150/289820","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/289820","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation-Reproduction (electronic)"},{"id":"mag:187733564","doi":null,"is_oa":false,"landing_page_url":"https://arizona.openrepository.com/handle/10150/289820","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":110,"referenced_works":["https://openalex.org/W46084203","https://openalex.org/W147875601","https://openalex.org/W153687226","https://openalex.org/W775019103","https://openalex.org/W1483350500","https://openalex.org/W1564392853","https://openalex.org/W1567065545","https://openalex.org/W1577210465","https://openalex.org/W1585650753","https://openalex.org/W1592545874","https://openalex.org/W1596804482","https://openalex.org/W1604214492","https://openalex.org/W1686581241","https://openalex.org/W1753843006","https://openalex.org/W1852817901","https://openalex.org/W1902330701","https://openalex.org/W1923904866","https://openalex.org/W1965816376","https://openalex.org/W1968553219","https://openalex.org/W1969198649","https://openalex.org/W1970361438","https://openalex.org/W1972870617","https://openalex.org/W1975262853","https://openalex.org/W1976542302","https://openalex.org/W1978086282","https://openalex.org/W1979293468","https://openalex.org/W1979385488","https://openalex.org/W1980605502","https://openalex.org/W1981493299","https://openalex.org/W1981891173","https://openalex.org/W1991473840","https://openalex.org/W1993587560","https://openalex.org/W1993878963","https://openalex.org/W1994986373","https://openalex.org/W1995147161","https://openalex.org/W1996285297","https://openalex.org/W1996466550","https://openalex.org/W1996995735","https://openalex.org/W2007174401","https://openalex.org/W2008206320","https://openalex.org/W2009192678","https://openalex.org/W2010051841","https://openalex.org/W2016878695","https://openalex.org/W2021979138","https://openalex.org/W2022199479","https://openalex.org/W2024398910","https://openalex.org/W2024454130","https://openalex.org/W2032425419","https://openalex.org/W2034115580","https://openalex.org/W2034395399","https://openalex.org/W2034825884","https://openalex.org/W2036258710","https://openalex.org/W2037444729","https://openalex.org/W2042729100","https://openalex.org/W2043578693","https://openalex.org/W2047826668","https://openalex.org/W2051152907","https://openalex.org/W2051260684","https://openalex.org/W2054119130","https://openalex.org/W2062565407","https://openalex.org/W2062670765","https://openalex.org/W2067441158","https://openalex.org/W2067477282","https://openalex.org/W2068367040","https://openalex.org/W2068877244","https://openalex.org/W2069066733","https://openalex.org/W2069818934","https://openalex.org/W2074853678","https://openalex.org/W2076789428","https://openalex.org/W2079051863","https://openalex.org/W2084043489","https://openalex.org/W2090599898","https://openalex.org/W2090845744","https://openalex.org/W2092014913","https://openalex.org/W2093385852","https://openalex.org/W2101413460","https://openalex.org/W2104969843","https://openalex.org/W2107258432","https://openalex.org/W2108111134","https://openalex.org/W2108711557","https://openalex.org/W2112120457","https://openalex.org/W2114707776","https://openalex.org/W2117514208","https://openalex.org/W2122054605","https://openalex.org/W2124939272","https://openalex.org/W2133354537","https://openalex.org/W2133691334","https://openalex.org/W2144001627","https://openalex.org/W2145242708","https://openalex.org/W2156437245","https://openalex.org/W2162027734","https://openalex.org/W2163364932","https://openalex.org/W2165590312","https://openalex.org/W2166285615","https://openalex.org/W2166320770","https://openalex.org/W2169316199","https://openalex.org/W2188537475","https://openalex.org/W2260577419","https://openalex.org/W2268369589","https://openalex.org/W2269570071","https://openalex.org/W2272342182","https://openalex.org/W2282014285","https://openalex.org/W2291476174","https://openalex.org/W2404575208","https://openalex.org/W2408984145","https://openalex.org/W2415238973","https://openalex.org/W2418252308","https://openalex.org/W4298446475","https://openalex.org/W4406065712","https://openalex.org/W4406968089"],"related_works":["https://openalex.org/W2145764581","https://openalex.org/W2046755498","https://openalex.org/W2142417997","https://openalex.org/W2028249028","https://openalex.org/W7095290321","https://openalex.org/W2163364932","https://openalex.org/W3168659301","https://openalex.org/W2112120457","https://openalex.org/W7151493314","https://openalex.org/W2103475169"],"abstract_inverted_index":{"We":[0,215],"are":[1],"studying":[2,158],"the":[3,6,24,66,81,97,103,106,121,124,148,209,220,241],"pharmacology":[4],"of":[5,43,75,80,105,117,123,130,174,208,222,226,236],"human":[7],"delta-opioid":[8,19,37,107],"receptor":[9,20,82],"stably":[10],"expressed":[11],"in":[12,46,92,102,143,254],"Chinese":[13],"hamster":[14],"ovary":[15],"cells":[16,48],"(hDOR/CHO).":[17],"The":[18,109,128],"mediates":[21],"analgesia,":[22],"without":[23],"negative":[25],"side":[26],"effects":[27],"noted":[28],"by":[29,53,171,188],"mu-":[30],"and":[31,147,179,197,206,250],"kappa-opioid":[32],"agonists.":[33],"However,":[34],"tolerance":[35,45],"to":[36,73,88,140,142,154,203,234],"agonists":[38],"still":[39],"occurs.":[40],"One":[41],"mechanism":[42],"drug":[44,145],"CHO":[47,255],"involves":[49],"a":[50,89,164],"compensatory":[51,90],"response":[52],"adenylyl":[54,60],"cyclase":[55,61],"VI.":[56],"This":[57],"response,":[58],"termed":[59],"(AC)":[62],"superactivation,":[63,196],"arises":[64],"from":[65],"observation":[67],"that":[68,198,232,245],"acute":[69,98],"hDOR":[70],"activation":[71,79,235],"leads":[72,87],"inhibition":[74,99,133],"AC,":[76],"whereas":[77],"chronic":[78,135,189],"(greater":[83],"than":[84],"4":[85],"hours)":[86],"increase":[91],"AC":[93,111,132,185,195,252],"activity,":[94],"effectively":[95],"negating":[96],"normally":[100],"seen":[101],"presence":[104],"agonist.":[108],"increased":[110],"activity":[112],"also":[113,217],"causes":[114],"an":[115],"overshoot":[116,151],"cAMP":[118,150,242],"formation":[119],"upon":[120],"removal":[122],"agonistic":[125],"inhibitory":[126],"influence.":[127],"loss":[129],"receptor-mediated":[131],"after":[134],"agonist":[136,190],"treatment":[137],"is":[138,201],"thought":[139],"contribute":[141,153],"vivo":[144],"tolerance,":[146],"resulting":[149],"may":[152,193],"opioid":[155],"withdrawal.":[156],"In":[157],"this":[159,199,237],"phenomenon,":[160],"we":[161,182],"have":[162,183,216],"demonstrated":[163],"requirement":[165],"for":[166],"G-protein":[167],"betagamma":[168],"subunits":[169],"(Gbetagamma)":[170],"expressing":[172],"scavengers":[173],"Gbetagamma,":[175],"such":[176],"as":[177,228,230],"alpha-transducin":[178],"phosducin.":[180],"Additionally,":[181],"shown":[184],"VI":[186,253],"phosphorylation":[187,200],"treatment,":[191],"which":[192],"cause":[194],"sensitive":[202],"calmodulin":[204],"antagonists":[205],"inhibitors":[207],"atypical":[210],"protein":[211],"kinase":[212],"C":[213],"enzymes.":[214],"recently":[218],"postulated":[219],"involvement":[221],"Raf-1":[223,247],"kinase.":[224],"Inhibitors":[225],"Raf-1,":[227],"well":[229],"pathways":[231],"lead":[233],"enzyme,":[238],"significantly":[239],"attenuate":[240],"overshoot,":[243],"suggesting":[244],"hDOR-activated":[246],"can":[248],"phosphorylate":[249],"superactivate":[251],"cells.":[256]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}