{"id":"https://openalex.org/W187258010","doi":"https://doi.org/10.1042/bj1410127","title":"Kinetic properties of rat liver pyruvate kinase at cellular concentrations of enzyme, substrates and modifiers","display_name":"Kinetic properties of rat liver pyruvate kinase at cellular concentrations of enzyme, substrates and modifiers","publication_year":1974,"publication_date":"1974-07-01","ids":{"openalex":"https://openalex.org/W187258010","doi":"https://doi.org/10.1042/bj1410127","mag":"187258010","pmid":"https://pubmed.ncbi.nlm.nih.gov/4455196","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1168057"},"language":"en","primary_location":{"id":"doi:10.1042/bj1410127","doi":"https://doi.org/10.1042/bj1410127","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj1410127","pdf_url":"https://portlandpress.com/biochemj/article-pdf/141/1/127/563210/bj1410127.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/141/1/127/563210/bj1410127.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059057737","display_name":"Wayne Flory","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115475287","display_name":"Oklahoma State University","ror":"https://ror.org/01g9vbr38","country_code":"US","type":"education","lineage":["https://openalex.org/I115475287"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wayne Flory","raw_affiliation_strings":["Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A","institution_ids":["https://openalex.org/I115475287"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030990474","display_name":"Benigno D. Peczon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115475287","display_name":"Oklahoma State University","ror":"https://ror.org/01g9vbr38","country_code":"US","type":"education","lineage":["https://openalex.org/I115475287"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benigno D. Peczon","raw_affiliation_strings":["Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A","institution_ids":["https://openalex.org/I115475287"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Roger E. Koeppe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115475287","display_name":"Oklahoma State University","ror":"https://ror.org/01g9vbr38","country_code":"US","type":"education","lineage":["https://openalex.org/I115475287"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Roger E. Koeppe","raw_affiliation_strings":["Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A","institution_ids":["https://openalex.org/I115475287"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043485914","display_name":"H. Olin Spivey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115475287","display_name":"Oklahoma State University","ror":"https://ror.org/01g9vbr38","country_code":"US","type":"education","lineage":["https://openalex.org/I115475287"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H. Olin Spivey","raw_affiliation_strings":["Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074, U.S.A","institution_ids":["https://openalex.org/I115475287"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I115475287"],"apc_list":null,"apc_paid":null,"fwci":1.5719,"has_fulltext":true,"cited_by_count":37,"citation_normalized_percentile":{"value":0.79729052,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"141","issue":"1","first_page":"127","last_page":"131"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.8949999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.8949999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12915","display_name":"Biochemical Acid Research Studies","score":0.03200000151991844,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.02070000022649765,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7649999856948853},{"id":"https://openalex.org/keywords/fructose-1-6-bisphosphate","display_name":"fructose-1,6-bisphosphate","score":0.9139999747276306},{"id":"https://openalex.org/keywords/allosteric-regulation","display_name":"allosteric regulation","score":0.7670000195503235},{"id":"https://openalex.org/keywords/mgatp","display_name":"MgATP","score":0.6269999742507935},{"id":"https://openalex.org/keywords/l-alanine","display_name":"L-alanine","score":0.7710000276565552},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.5329999923706055},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.640999972820282},{"id":"https://openalex.org/keywords/rat-liver","display_name":"rat liver","score":0.5590000152587891},{"id":"https://openalex.org/keywords/glycolysis","display_name":"glycolysis","score":0.5070000290870667}],"concepts":[{"id":"https://openalex.org/C2780854706","wikidata":"https://www.wikidata.org/wiki/Q900970","display_name":"Pyruvate kinase","level":4,"score":0.9186187982559204},{"id":"https://openalex.org/C190098323","wikidata":"https://www.wikidata.org/wiki/Q14912712","display_name":"Pyruvate dehydrogenase phosphatase","level":4,"score":0.7385357618331909},{"id":"https://openalex.org/C10730799","wikidata":"https://www.wikidata.org/wiki/Q18030568","display_name":"PKM2","level":5,"score":0.7365835905075073},{"id":"https://openalex.org/C143937172","wikidata":"https://www.wikidata.org/wiki/Q303568","display_name":"Phosphoenolpyruvate carboxykinase","level":3,"score":0.7062523365020752},{"id":"https://openalex.org/C9506865","wikidata":"https://www.wikidata.org/wiki/Q6321025","display_name":"Pyruvate dehydrogenase kinase","level":4,"score":0.6737436056137085},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6624339818954468},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6553053855895996},{"id":"https://openalex.org/C101972281","wikidata":"https://www.wikidata.org/wiki/Q899965","display_name":"Pyruvate decarboxylation","level":4,"score":0.6466280221939087},{"id":"https://openalex.org/C2776970464","wikidata":"https://www.wikidata.org/wiki/Q122043","display_name":"Fructose","level":2,"score":0.6395056843757629},{"id":"https://openalex.org/C140137476","wikidata":"https://www.wikidata.org/wiki/Q21114968","display_name":"Pyruvate carboxylase","level":3,"score":0.535101056098938},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46942904591560364},{"id":"https://openalex.org/C88099638","wikidata":"https://www.wikidata.org/wiki/Q412856","display_name":"Pyruvate dehydrogenase complex","level":3,"score":0.4530968964099884},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4395189881324768},{"id":"https://openalex.org/C20251656","wikidata":"https://www.wikidata.org/wiki/Q162643","display_name":"Glycolysis","level":3,"score":0.43792539834976196},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4129115641117096},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34663310647010803}],"mesh":[{"descriptor_ui":"D000244","descriptor_name":"Adenosine Diphosphate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000409","descriptor_name":"Alanine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002848","descriptor_name":"Chromatography, DEAE-Cellulose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005636","descriptor_name":"Fructosephosphates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005994","descriptor_name":"Glycerophosphates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008274","descriptor_name":"Magnesium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010728","descriptor_name":"Phosphoenolpyruvate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011770","descriptor_name":"Pyruvate Kinase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D011773","descriptor_name":"Pyruvates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1042/bj1410127","doi":"https://doi.org/10.1042/bj1410127","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj1410127","pdf_url":"https://portlandpress.com/biochemj/article-pdf/141/1/127/563210/bj1410127.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1168057","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1168057","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:4455196","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4455196","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1042/bj1410127","doi":"https://doi.org/10.1042/bj1410127","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj1410127","pdf_url":"https://portlandpress.com/biochemj/article-pdf/141/1/127/563210/bj1410127.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320306095","display_name":"American Cancer Society","ror":"https://ror.org/02e463172"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"},{"id":"https://openalex.org/F4320332866","display_name":"Oklahoma Agricultural Experiment Station","ror":"https://ror.org/01g9vbr38"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W187258010.pdf","grobid_xml":"https://content.openalex.org/works/W187258010.grobid-xml"},"referenced_works_count":19,"referenced_works":["https://openalex.org/W26309257","https://openalex.org/W1502101722","https://openalex.org/W1511922886","https://openalex.org/W1533366558","https://openalex.org/W1533618603","https://openalex.org/W1570385971","https://openalex.org/W1775749144","https://openalex.org/W1863850981","https://openalex.org/W1904193938","https://openalex.org/W1964883811","https://openalex.org/W2021920512","https://openalex.org/W2099129407","https://openalex.org/W2122638544","https://openalex.org/W2123598700","https://openalex.org/W2165484107","https://openalex.org/W2317739944","https://openalex.org/W2324672218","https://openalex.org/W2345214698","https://openalex.org/W6610205693"],"related_works":["https://openalex.org/W2053552604","https://openalex.org/W2047746451","https://openalex.org/W1511922886","https://openalex.org/W2059712364","https://openalex.org/W1999883047","https://openalex.org/W2094172422","https://openalex.org/W2014631913","https://openalex.org/W2069903761","https://openalex.org/W2276916325","https://openalex.org/W1959881753"],"abstract_inverted_index":{"Kinetic":[0],"properties":[1,36],"of":[2,18,66,74,85,95,103,121,127],"rat":[3],"liver":[4],"pyruvate":[5,75,87,113,131],"kinase":[6,76,88,114],"type":[7],"I":[8,77,89],"at":[9,24,78,134],"pH7.5":[10],"and":[11,21,47,68],"6.5":[12,110],"were":[13],"studied":[14],"with":[15,51,57],"physiological":[16,101],"ranges":[17],"substrates,":[19],"modifiers":[20],"Mg(2+)":[22],"concentrations":[23,32,65,136],"increasing":[25],"enzyme":[26,41,52,96,139],"concentrations,":[27],"including":[28],"the":[29,55,92,99,107,125,138],"estimated":[30],"cellular":[31,64,83,135],"(approx.":[33],"0.1mg/ml).":[34],"Enzyme":[35],"appear":[37],"unaffected":[38],"by":[39,98],"increased":[40],"concentration":[42],"if":[43],"phosphoenolpyruvate,":[44],"fructose":[45,104,128],"1,6-diphosphate":[46],"inhibitors":[48],"are":[49],"incubated":[50],"before":[53],"starting":[54],"reaction":[56],"ADP.":[58],"Our":[59],"data":[60],"suggest":[61],"that":[62],"minimum":[63],"MgATP":[67],"l-alanine":[69],"provide":[70],"virtually":[71],"complete":[72],"inhibition":[73],"pH7.5.":[79],"The":[80],"most":[81],"likely":[82],"control":[84],"existing":[86],"results":[90],"from":[91],"strong":[93],"restoration":[94],"activity":[97,123],"small":[100],"amounts":[102],"1,6-diphosphate.":[105,129],"Decreasing":[106],"pH":[108],"to":[109,117],"also":[111],"restores":[112],"activity,":[115],"but":[116],"only":[118],"about":[119],"one-third":[120],"its":[122],"in":[124],"presence":[126],"Neither":[130],"nor":[132],"2-phosphoglycerate":[133],"inhibit":[137],"significantly.":[140]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}