{"id":"https://openalex.org/W1868650648","doi":null,"title":"Genome Engineering Technology and Its Application in Mammalian Cells","display_name":"Genome Engineering Technology and Its Application in Mammalian Cells","publication_year":2014,"publication_date":"2014-06-06","ids":{"openalex":"https://openalex.org/W1868650648","mag":"1868650648"},"language":"en","primary_location":{"id":"pmh:oai:dash.harvard.edu:1/12274330","doi":null,"is_oa":false,"landing_page_url":"http://dissertations.umi.com/gsas.harvard:11309","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis or Dissertation"},"volume":null,"type":"dissertation","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080765172","display_name":"Le Cong","orcid":"https://orcid.org/0000-0003-4725-8714","observed_orcids":["https://orcid.org/0000-0003-4725-8714"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Le Cong","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4725-8714","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5080765172"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.9760000109672546},{"id":"https://openalex.org/keywords/mammalian-cells","display_name":"mammalian cells","score":0.9879999756813049},{"id":"https://openalex.org/keywords/biomaterials","display_name":"biomaterials","score":0.5680000185966492}],"concepts":[{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.5796218514442444},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4790724217891693},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4571433365345001},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43257761001586914},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.37047892808914185},{"id":"https://openalex.org/C2522767166","wikidata":"https://www.wikidata.org/wiki/Q2374463","display_name":"Data science","level":1,"score":0.3675386905670166},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.28544938564300537},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.264889121055603},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.16648408770561218},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12700438499450684}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:dash.harvard.edu:1/12274330","doi":null,"is_oa":false,"landing_page_url":"http://dissertations.umi.com/gsas.harvard:11309","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis or Dissertation"},{"id":"mag:1868650648","doi":null,"is_oa":false,"landing_page_url":"https://dash.harvard.edu/handle/1/12274330","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":151,"referenced_works":["https://openalex.org/W35995934","https://openalex.org/W193111756","https://openalex.org/W795187385","https://openalex.org/W1578187492","https://openalex.org/W1970893992","https://openalex.org/W1972255439","https://openalex.org/W1976508887","https://openalex.org/W1977036449","https://openalex.org/W1978094899","https://openalex.org/W1978129709","https://openalex.org/W1980556586","https://openalex.org/W1981476536","https://openalex.org/W1981521195","https://openalex.org/W1983166726","https://openalex.org/W1983792557","https://openalex.org/W1983858690","https://openalex.org/W1983926199","https://openalex.org/W1985195841","https://openalex.org/W1987188990","https://openalex.org/W1988457474","https://openalex.org/W1988508505","https://openalex.org/W1988840491","https://openalex.org/W1989454102","https://openalex.org/W1990319434","https://openalex.org/W1991062831","https://openalex.org/W1991200056","https://openalex.org/W1991389689","https://openalex.org/W1991710875","https://openalex.org/W1994390268","https://openalex.org/W1995280535","https://openalex.org/W1997250478","https://openalex.org/W1999068651","https://openalex.org/W2001368885","https://openalex.org/W2001831734","https://openalex.org/W2002767268","https://openalex.org/W2004360848","https://openalex.org/W2008183439","https://openalex.org/W2008553597","https://openalex.org/W2009626008","https://openalex.org/W2010986636","https://openalex.org/W2013850452","https://openalex.org/W2014850190","https://openalex.org/W2016667239","https://openalex.org/W2016745966","https://openalex.org/W2016955606","https://openalex.org/W2019670881","https://openalex.org/W2020026526","https://openalex.org/W2020091112","https://openalex.org/W2022016707","https://openalex.org/W2022321112","https://openalex.org/W2023186109","https://openalex.org/W2025074364","https://openalex.org/W2026871784","https://openalex.org/W2027387232","https://openalex.org/W2028603053","https://openalex.org/W2029425876","https://openalex.org/W2032387485","https://openalex.org/W2033433686","https://openalex.org/W2034252391","https://openalex.org/W2037174767","https://openalex.org/W2037566247","https://openalex.org/W2039316426","https://openalex.org/W2039865022","https://openalex.org/W2042758414","https://openalex.org/W2044741494","https://openalex.org/W2045435533","https://openalex.org/W2047032063","https://openalex.org/W2050757906","https://openalex.org/W2051395442","https://openalex.org/W2053558244","https://openalex.org/W2056026468","https://openalex.org/W2056793372","https://openalex.org/W2058304957","https://openalex.org/W2059128096","https://openalex.org/W2060075809","https://openalex.org/W2062119305","https://openalex.org/W2064815984","https://openalex.org/W2065027194","https://openalex.org/W2068847708","https://openalex.org/W2073377349","https://openalex.org/W2073873559","https://openalex.org/W2074353166","https://openalex.org/W2074579392","https://openalex.org/W2075529916","https://openalex.org/W2078308455","https://openalex.org/W2081636325","https://openalex.org/W2082131511","https://openalex.org/W2082182783","https://openalex.org/W2083582836","https://openalex.org/W2085516892","https://openalex.org/W2085819365","https://openalex.org/W2087264052","https://openalex.org/W2087879073","https://openalex.org/W2088816616","https://openalex.org/W2090701628","https://openalex.org/W2091708974","https://openalex.org/W2091776576","https://openalex.org/W2092806325","https://openalex.org/W2097049188","https://openalex.org/W2098448867","https://openalex.org/W2099106733","https://openalex.org/W2101027021","https://openalex.org/W2102484627","https://openalex.org/W2102560467","https://openalex.org/W2104809172","https://openalex.org/W2107077624","https://openalex.org/W2107696332","https://openalex.org/W2107900025","https://openalex.org/W2108244474","https://openalex.org/W2110260408","https://openalex.org/W2112006537","https://openalex.org/W2112295554","https://openalex.org/W2115268444","https://openalex.org/W2116565356","https://openalex.org/W2118149818","https://openalex.org/W2120503144","https://openalex.org/W2123502778","https://openalex.org/W2124990349","https://openalex.org/W2125987139","https://openalex.org/W2126942185","https://openalex.org/W2130069008","https://openalex.org/W2130931930","https://openalex.org/W2132256310","https://openalex.org/W2132291850","https://openalex.org/W2133704193","https://openalex.org/W2135598273","https://openalex.org/W2137209165","https://openalex.org/W2143554177","https://openalex.org/W2143774753","https://openalex.org/W2144249817","https://openalex.org/W2144269887","https://openalex.org/W2144779133","https://openalex.org/W2146370769","https://openalex.org/W2149071011","https://openalex.org/W2149751339","https://openalex.org/W2150935817","https://openalex.org/W2157645703","https://openalex.org/W2158023793","https://openalex.org/W2158308376","https://openalex.org/W2161974563","https://openalex.org/W2163241233","https://openalex.org/W2164330524","https://openalex.org/W2164618384","https://openalex.org/W2164852115","https://openalex.org/W2167639593","https://openalex.org/W2168245648","https://openalex.org/W2169064463","https://openalex.org/W2171586071","https://openalex.org/W2326434797","https://openalex.org/W2397885344","https://openalex.org/W2410626038"],"related_works":["https://openalex.org/W2026788452","https://openalex.org/W2535341543","https://openalex.org/W3172702098","https://openalex.org/W2001439073","https://openalex.org/W4213361471","https://openalex.org/W2467967955","https://openalex.org/W2697347174","https://openalex.org/W2028650313","https://openalex.org/W6989931658","https://openalex.org/W2346906262"],"abstract_inverted_index":{"The":[0],"advancement":[1],"of":[2,14,17,27,85],"high-throughput,":[3],"large-scale":[4],"biochemical,":[5],"biophysical,":[6],"and":[7,20,41,49,56,79,87],"genetic":[8],"technologies":[9],"has":[10],"enabled":[11,33],"the":[12,46,64,73,83],"generation":[13],"massive":[15],"amounts":[16],"biological":[18,55,92],"data":[19],"allowed":[21],"us":[22,38,62],"to":[23,39,54,67,71,82,90,103],"synthesize":[24],"various":[25],"types":[26],"biomaterial":[28],"for":[29,37],"engineering":[30],"purposes.":[31],"This":[32],"improved":[34],"observational":[35],"methodologies":[36],"navigate":[40],"locate,":[42],"with":[43,63],"unprecedented":[44],"resolution,":[45],"potential":[47],"factors":[48],"connections":[50],"that":[51],"may":[52],"contribute":[53],"biomedical":[57],"processes.":[58],"Nonetheless,":[59],"it":[60],"leaves":[61],"increasing":[65],"demand":[66],"validate":[68],"these":[69,97],"observations":[70],"elucidate":[72],"actual":[74],"causal":[75],"mechanisms":[76],"in":[77,94],"biology":[78],"medicine.":[80],"Due":[81],"lack":[84],"powerful":[86],"precise":[88],"tools":[89],"manipulate":[91],"systems":[93],"mammalian":[95],"cells,":[96],"efforts":[98],"have":[99],"not":[100],"been":[101],"able":[102],"progress":[104],"at":[105],"an":[106],"adequate":[107],"pace.":[108]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2016-06-24T00:00:00"}
