{"id":"https://openalex.org/W1864767661","doi":null,"title":"Infection of human embryonic cell cultures with the Rauscher murine leukemia virus.","display_name":"Infection of human embryonic cell cultures with the Rauscher murine leukemia virus.","publication_year":1969,"publication_date":"1969-10-01","ids":{"openalex":"https://openalex.org/W1864767661","mag":"1864767661","pmid":"https://pubmed.ncbi.nlm.nih.gov/4980925"},"language":"en","primary_location":{"id":"pmid:4980925","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4980925","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017900670","display_name":"Bruce S. Wright","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B S Wright","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5016175277","display_name":"Wlo Korol","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W Korol","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.1049,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.85511111,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"29","issue":"10","first_page":"1886","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rauscher-murine-leukemia-virus","display_name":"Rauscher leukemia virus","score":0.9890000224113464},{"id":"https://openalex.org/keywords/c-type-particles","display_name":"C-type particles","score":0.6060000061988831},{"id":"https://openalex.org/keywords/virus-budding","display_name":"viral budding","score":0.4869999885559082},{"id":"https://openalex.org/keywords/cytopathic-effect","display_name":"cytopathic effect","score":0.6639999747276306},{"id":"https://openalex.org/keywords/oncogenic-rna-viruses","display_name":"oncogenic RNA viruses","score":0.6980000138282776},{"id":"https://openalex.org/keywords/electron-microscopy-of-infected-cells","display_name":"electron microscopy of infected cells","score":0.6460000276565552}],"concepts":[{"id":"https://openalex.org/C2779562191","wikidata":"https://www.wikidata.org/wiki/Q673848","display_name":"Murine leukemia virus","level":3,"score":0.6822988390922546},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6697579026222229},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.649138867855072},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.639485239982605},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.6317855715751648},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.5872361660003662},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5775381922721863},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.573814868927002},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.25023049116134644},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.16907444596290588},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003168","descriptor_name":"Complement Fixation Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005779","descriptor_name":"Immunodiffusion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007181","descriptor_name":"Inclusion Bodies, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009132","descriptor_name":"Muscles","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D011925","descriptor_name":"Rauscher Virus","qualifier_ui":"Q000472","qualifier_name":"pathogenicity","is_major_topic":true},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:4980925","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4980925","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"pmh:oai:mouseion.jax.org:ssbb1969-2482","doi":null,"is_oa":false,"landing_page_url":"https://mouseion.jax.org/ssbb1969/1483","pdf_url":null,"source":{"id":"https://openalex.org/S4377196421","display_name":"The Mouseion at the JAXlibrary (Jackson Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210115256","host_organization_name":"Jackson Laboratory","host_organization_lineage":["https://openalex.org/I4210115256"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Subject Strain Bibliography 1969","raw_type":"text"},{"id":"mag:1864767661","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4980925","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1515274028","https://openalex.org/W1536813579","https://openalex.org/W1585894187","https://openalex.org/W1915937367","https://openalex.org/W1979058305","https://openalex.org/W1981324523","https://openalex.org/W2017638565","https://openalex.org/W2042752766","https://openalex.org/W2090879212","https://openalex.org/W2302434084"],"related_works":["https://openalex.org/W2606484039","https://openalex.org/W7097568405","https://openalex.org/W7214270862","https://openalex.org/W2138251806","https://openalex.org/W7111873114","https://openalex.org/W2302434084","https://openalex.org/W2319528857","https://openalex.org/W2314529594","https://openalex.org/W2099033957","https://openalex.org/W128350768"],"abstract_inverted_index":{"The":[0,392,551,611,622,686,705],"John":[1],"L.":[2],"Smith":[3],"Memorial":[4],"for":[5,202,433,664,714],"Cancer":[6],"Research,":[7],"Chas.":[8],"Pfizer":[9],"&":[10],"Company,":[11],"Inc.,":[12],"Maywood,":[13],"New":[14],"Jersey":[15],"07607":[16],"Although":[17],"infection":[18,39,59,111,339,439],"of":[19,40,60,80,89,104,112,126,132,142,166,241,250,294,320,358,383,440,464,476,482,495,572,579,645,722,733,742],"mouse":[20,167,172,434,501,528,659,692],"cell":[21,42,115,442,502,614],"lines":[22],"with":[23,32,68,117,154,161,215,461,492,525,581,750],"murine":[24,70,120,301],"leukemia":[25,121,507,688],"and":[26,77,124,129,195,213,224,438,453,546],"sarcoma":[27,71],"viruses":[28,84],"can":[29],"be":[30,96,523],"achieved":[31],"relative":[33],"ease":[34],"(4,":[35],"6,":[36],"12),":[37],"similar":[38,752],"human":[41,63,86,105,113,143,597,613,650,674,717],"cultures":[43,116,137,197,229,443,503,548],"has":[44,479,504],"not":[45,222,246],"been":[46],"reported":[47],"until":[48],"very":[49],"recently.":[50],"Boiron":[51],"et":[52,567],"al.":[53,568],"(2)":[54],"have":[55,422],"described":[56,564],"the":[57,69,93,101,110,118,127,133,139,196,208,216,228,242,248,260,269,286,292,295,300,313,321,353,359,381,449,469,477,483,562,582,596,603,628,646,665,672,679,715,755],"successful":[58],"a":[61,97,331,375,409,526,608,635,642,683,734,743,751],"diploid":[62],"embryonic":[64,114,144],"lung":[65],"culture,":[66],"WI-38,":[67],"virus":[72,122,134,174,206,251,263,308,327,393,427,466,486,497,530,559,599,605,647,661,670,681,689,724,736],"Moloney":[73],"strain":[74],"(MSV-M).":[75],"Infection":[76],"continuous":[78,377],"replication":[79],"animal":[81],"oncogenic":[82],"RNA":[83],"in":[85,149,181,189,285,299,318,328,334,374,396,444,555,619,625,649,691,702],"cells":[87,244,592,675,693],"is":[88],"considerable":[90],"interest":[91],"as":[92,274,276,549],"phenomenon":[94],"may":[95],"step":[98],"towards":[99],"proving":[100],"viral":[102],"etiology":[103],"leukemia.":[106],"This":[107,747],"report":[108],"describes":[109],"Rauscher":[119,485,496,527,687],"(RLV)":[123],"some":[125],"physical":[128],"biologic":[130],"properties":[131],"synthesized.":[135],"Monolayer":[136],"from":[138,268,330,499,544,671],"4th":[140],"transfer":[141,337],"muscle":[145],"cells,":[146],"G8-6,":[147],"growing":[148],"RPMI":[150],"medium":[151,183,329,399],"#1629":[152],"supplemented":[153],"20%":[155],"fetal":[156],"calf":[157],"serum":[158,656],"were":[159,198,210,221,230,265,310,415,431,458,539,738],"washed":[160,211],"fresh":[162,398],"medium.":[163,218],"One":[164],"ml":[165,463,732],"plasma":[168,173,270,529,660],"virus,":[169],"HL-67-4":[170],"(Rauscher":[171],"supplied":[175],"by":[176,312,342,417,565,589,634,727],"Hazleton":[177],"Laboratories)":[178],"diluted":[179],"1:4":[180,609],"growth":[182,217],"was":[184,340,345,394,553,574,587,617,632,662,699,711,725],"added":[185],"to":[186,232,407,421,447,512,531,561,640],"each":[187],"monolayer":[188],"30-ml":[190],"plastic":[191],"tissue":[192,235,323],"culture":[193,236,324,333,615],"flasks,":[194],"incubated":[199],"at":[200,252,380],"37~":[201],"30":[203],"minutes.":[204],"After":[205,517],"adsorption,":[207],"monolayers":[209],"once":[212],"refed":[214],"Cytopathic":[219],"effects":[220],"observed,":[223],"after":[225,256],"one":[226,384,511,753],"week":[227],"transferred":[231],"250-rnl":[233],"Falcon":[234],"flasks.":[237],"Electron":[238],"microscopic":[239,419,707],"studies":[240,420],"infected":[243,322,500,590,673],"did":[245],"reveal":[247],"presence":[249],"that":[253,506,586],"time;":[254],"however,":[255],"four":[257,577],"weeks,":[258],"during":[259],"17th":[261],"passage,":[262],"particles":[264,283,297,309,428],"seen":[266,311],"budding":[267],"membranes":[271],"(Fig.":[272,280,289],"1A)":[273],"well":[275],"into":[277],"cytoplasmic":[278],"vacuoles":[279],"1B).":[281],"Virus":[282,355],"found":[284,298],"extracellular":[287],"spaces":[288],"1C)":[290],"had":[291,602],"morphology":[293],"C-type":[296],"leukemias":[302],"(1).":[303],"In":[304,594],"subsequent":[305],"passages,":[306],"many":[307],"negative":[314,618,684,713],"stain":[315],"technic":[316],"(3)":[317],"concentrates":[319,543],"fluids.":[325],"Extracellular":[326],"5-day-old":[332],"its":[335],"25th":[336],"since":[338],"concentrated":[341],"1This":[343],"study":[344],"conducted":[346,540],"under":[347],"contract":[348],"No.":[349],"PH":[350],"43-66-98":[351],"within":[352,510],"Special":[354],"Leukemia":[356],"Program":[357],"NIH,":[360],"USPHS,":[361],"Bethesda,":[362],"Maryland":[363],"20014.":[364],"Received":[365],"May":[366],"5,":[367],"1969;":[368],"accepted":[369],"June":[370],"13,":[371],"1969.":[372],"ultracentrifugation":[373],"Sharples":[376],"flow":[378],"centrifuge":[379],"rate":[382],"liter":[385],"per":[386,429],"hour":[387],"(approx.":[388],"55,000":[389],"X":[390,425],"g).":[391],"resuspended":[395],"either":[397],"or":[400,697],"0.05":[401],"M":[402],"sodium":[403],"citrate,":[404],"pH":[405],"6.8,":[406],"give":[408],"100-fold":[410,542],"concentrate.":[411],"These":[412],"preparations,":[413],"which":[414,601,631],"estimated":[416],"electron":[418,706],"approximately":[423],"5":[424],"109":[426],"ml,":[430],"used":[432,663],"inoculation,":[435],"serologic":[436],"tests,":[437],"other":[441],"an":[445],"effort":[446],"characterize":[448],"virus.":[450,719],"Sixty":[451],"newborn":[452],"ten":[454],"weanling":[455],"BALB/c":[456],"mice":[457,478,521],"inoculated":[459],"intraperitoneally":[460],"0.1":[462],"this":[465,620,723],"preparation.":[467],"At":[468],"present":[470],"time,":[471],"three":[472],"months":[473,514,519],"postinoculation,":[474],"none":[475],"shown":[480,505],"any":[481],"typical":[484],"disease":[487],"symptoms":[488],"(8).":[489],"Previous":[490],"experience":[491],"equivalent":[493],"doses":[494],"derived":[498],"should":[508],"develop":[509],"two":[513,629],"(5,":[515],"10).":[516],"six":[518],"these":[520,703],"will":[522],"challenged":[524],"determine":[532],"their":[533],"immune":[534],"status.":[535],"Complement":[536],"fixation":[537],"tests":[538],"using":[541],"control":[545,584],"virus-infected":[547],"antigens.":[550],"antiserum":[552],"prepared":[554,657],"rats":[556],"against":[557,576,658],"mouse-derived":[558],"according":[560],"procedure":[563],"Hartley":[566],"(5).":[569],"A":[570,652],"titer":[571],"1:16":[573],"obtained":[575],"units":[578],"antibody":[580],"positive":[583,701],"RLV":[585],"produced":[588,648,676,690],"JLS-V9":[591],"(11).":[593],"contrast,":[595],"cell-derived":[598,718],"concentrate,":[600],"same":[604],"count,":[606],"gave":[607],"titer.":[610],"uninfected":[612],"concentrate":[616,737],"test.":[621],"four-fold":[623],"difference":[624],"titers":[626],"between":[627],"antigens,":[630],"confirmed":[633],"repeat":[636],"test,":[637],"would":[638],"seem":[639],"indicate":[641],"modified":[643],"nature":[644],"cells.":[651],"potent":[653],"rabbit":[654],"anti-RLV":[655],"immunodiffusion":[666],"tests.":[667,704],"While":[668],"ether-treated":[669,696],"precipitin":[677],"lines,":[678],"intact":[680,716],"showed":[682],"reaction.":[685],"(JLS-V9),":[694],"whether":[695],"intact,":[698],"always":[700],"agglutination":[708],"test":[709],"(7)":[710],"also":[712],"Further":[720],"characterization":[721],"attempted":[726],"density":[728],"gradient":[729],"centrifugation.":[730],"Two":[731],"50-fold":[735],"layered":[739],"on":[740],"top":[741],"preformed":[744],"sucrose":[745],"gradient.":[746],"gradient,":[748],"along":[749],"containing":[754],"1886":[756],"CANCER":[757],"RESEARCH":[758],"VOL.":[759],"29":[760]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}