{"id":"https://openalex.org/W182723788","doi":"https://doi.org/10.1096/fasebj.21.6.a890","title":"Angiotensin AT1a shRNA Demonstrates Interactions between Brainstem Angiotensin AT1a Receptors and Angiotensin Converting Enzyme 2","display_name":"Angiotensin AT1a shRNA Demonstrates Interactions between Brainstem Angiotensin AT1a Receptors and Angiotensin Converting Enzyme 2","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W182723788","doi":"https://doi.org/10.1096/fasebj.21.6.a890","mag":"182723788"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.21.6.a890","doi":"https://doi.org/10.1096/fasebj.21.6.a890","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.21.6.a890","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102896378","display_name":"Zhanyi Lin","orcid":"https://orcid.org/0009-0006-8239-8690","observed_orcids":["https://orcid.org/0009-0006-8239-8690","https://orcid.org/0000-0002-7114-885X"]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]},{"id":"https://openalex.org/I4210153930","display_name":"Guangdong Provincial People's Hospital","ror":"https://ror.org/045kpgw45","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210153930"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Zhanyi Lin","raw_affiliation_strings":["Guangdong Cardiovascular Institute Guangdong Provincial People\u2019s Hospital 91 Dongchuan Road Guangzhou 210080 China, People\u2019s Republic of","Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guangdong Cardiovascular Institute Guangdong Provincial People\u2019s Hospital 91 Dongchuan Road Guangzhou 210080 China, People\u2019s Republic of","institution_ids":["https://openalex.org/I4210153930"]},{"raw_affiliation_string":"Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","institution_ids":["https://openalex.org/I19648265"]},{"raw_affiliation_string":"Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435","institution_ids":["https://openalex.org/I19648265"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041825352","display_name":"Wenfeng Zhang","orcid":"https://orcid.org/0000-0002-6113-0285","observed_orcids":["https://orcid.org/0000-0002-6113-0285"]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wenfeng Zhang","raw_affiliation_strings":["Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","institution_ids":["https://openalex.org/I19648265"]},{"raw_affiliation_string":"Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435","institution_ids":["https://openalex.org/I19648265"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051623155","display_name":"Mary P. Key","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mary key","raw_affiliation_strings":["Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","institution_ids":["https://openalex.org/I19648265"]},{"raw_affiliation_string":"Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435","institution_ids":["https://openalex.org/I19648265"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102206830","display_name":"Shuguang Lin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210153930","display_name":"Guangdong Provincial People's Hospital","ror":"https://ror.org/045kpgw45","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210153930"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shuguang Lin","raw_affiliation_strings":["Guangdong Cardiovascular Institute Guangdong Provincial People\u2019s Hospital 91 Dongchuan Road Guangzhou 210080 China, People\u2019s Republic of"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guangdong Cardiovascular Institute Guangdong Provincial People\u2019s Hospital 91 Dongchuan Road Guangzhou 210080 China, People\u2019s Republic of","institution_ids":["https://openalex.org/I4210153930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000755272","display_name":"Alex F. Chen","orcid":"https://orcid.org/0000-0002-9792-0916","observed_orcids":["https://orcid.org/0000-0002-9792-0916"]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alex F. Chen","raw_affiliation_strings":["Pharmacology and Neurology, Michigan State University College of Human Medicine, B403 Life Sciences Building, East Lansing, MI, 48824"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology and Neurology, Michigan State University College of Human Medicine, B403 Life Sciences Building, East Lansing, MI, 48824","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101734796","display_name":"Yanfang Chen","orcid":"https://orcid.org/0000-0003-4153-4669","observed_orcids":["https://orcid.org/0000-0003-4153-4669","https://orcid.org/0009-0001-6388-2851","https://orcid.org/0000-0003-1982-6243"]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yanfang Chen","raw_affiliation_strings":["Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","institution_ids":["https://openalex.org/I19648265"]},{"raw_affiliation_string":"Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435","institution_ids":["https://openalex.org/I19648265"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103333777","display_name":"Mariana Morris","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mariana Morris","raw_affiliation_strings":["Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology &amp; Toxicology Wright State University Boonshoft School of Medicine 3640 Colonel Glenn Highway Dayton OH 45435","institution_ids":["https://openalex.org/I19648265"]},{"raw_affiliation_string":"Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, 3640 Colonel Glenn Highway, Dayton, OH, 45435","institution_ids":["https://openalex.org/I19648265"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.2234,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.62477116,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"6","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11001","display_name":"Renin-Angiotensin System Studies","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11001","display_name":"Renin-Angiotensin System Studies","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14486","display_name":"Cardiovascular, Neuropeptides, and Oxidative Stress Research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/angiotensin-converting-enzyme-2","display_name":"angiotensin-converting enzyme 2","score":0.9580000042915344},{"id":"https://openalex.org/keywords/dorsal-vagal-complex","display_name":"dorsal vagal complex","score":0.9449999928474426},{"id":"https://openalex.org/keywords/brainstem","display_name":"brainstem","score":0.9819999933242798},{"id":"https://openalex.org/keywords/shrna","display_name":"shRNA","score":0.6129999756813049},{"id":"https://openalex.org/keywords/adenoviral-gene-transfer","display_name":"adenoviral gene transfer","score":0.6700000166893005},{"id":"https://openalex.org/keywords/receptor-mrna-expression","display_name":"receptor mRNA expression","score":0.7820000052452087},{"id":"https://openalex.org/keywords/at1b-receptor","display_name":"AT1B receptor","score":0.8360000252723694},{"id":"https://openalex.org/keywords/ace1","display_name":"ACE1","score":0.9779999852180481},{"id":"https://openalex.org/keywords/blood-pressure-regulation","display_name":"blood pressure regulation","score":0.8930000066757202},{"id":"https://openalex.org/keywords/autonomic-function","display_name":"autonomic function","score":0.9739999771118164}],"concepts":[{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.9158594012260437},{"id":"https://openalex.org/C2908929049","wikidata":"https://www.wikidata.org/wiki/Q65963433","display_name":"Angiotensin II","level":3,"score":0.6545698642730713},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.6055757999420166},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.548723578453064},{"id":"https://openalex.org/C198710026","wikidata":"https://www.wikidata.org/wiki/Q898218","display_name":"Renin\u2013angiotensin system","level":3,"score":0.5134297609329224},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.5097913146018982},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.4999396800994873},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.48026394844055176},{"id":"https://openalex.org/C551621295","wikidata":"https://www.wikidata.org/wiki/Q182228","display_name":"Brainstem","level":2,"score":0.42784371972084045},{"id":"https://openalex.org/C104849204","wikidata":"https://www.wikidata.org/wiki/Q24778533","display_name":"Angiotensin receptor","level":4,"score":0.42438194155693054},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40331047773361206},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3929661512374878},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.24609339237213135},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.18426629900932312},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1172497570514679},{"id":"https://openalex.org/C84393581","wikidata":"https://www.wikidata.org/wiki/Q82642","display_name":"Blood pressure","level":2,"score":0.11165615916252136},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.057956188917160034}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.21.6.a890","doi":"https://doi.org/10.1096/fasebj.21.6.a890","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.21.6.a890","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1486361117","https://openalex.org/W798991114","https://openalex.org/W2101713927","https://openalex.org/W3174174687","https://openalex.org/W2279932595","https://openalex.org/W3175822425","https://openalex.org/W1978933131","https://openalex.org/W1972878509","https://openalex.org/W2356072728","https://openalex.org/W1975658214"],"abstract_inverted_index":{"Angiotensin":[0],"(Ang)":[1],"AT1a":[2,29,36,138,190,193],"receptors":[3,110],"and":[4,37,125,131,186,212],"Ang":[5,120,122,137],"converting":[6],"enzyme":[7],"2(ACE2)":[8],"are":[9,111],"expressed":[10],"in":[11,43,61,70,207],"the":[12,33,54,71,146,163,205],"brainstem":[13,197],"dorsal":[14],"vagal":[15],"complex":[16],"(DVC).":[17],"Studies":[18],"were":[19,58,99],"conducted":[20],"using":[21,60,128],"adenovirus":[22],"mediated":[23],"inference":[24],"small":[25],"hairpin":[26],"RNA":[27],"for":[28,89,119],"(Ad\u2010AT1a\u2010shRNA)":[30],"to":[31,113],"evaluate":[32],"interactions":[34,203],"between":[35,204],"ACE2.":[38],"We":[39],"used":[40],"a":[41,66,90,106],"system":[42],"which":[44,109],"Ad\u2010AT1a\u2010shRNA":[45,73,135,150,164,173,183],"or":[46,74],"its":[47],"Ad\u2010LacZ":[48,75],"control":[49,147,208],"was":[50,83,117,167],"injected":[51],"bilaterally":[52],"into":[53],"DVC.":[55],"mRNA":[56,96,139,157,160],"levels":[57],"measured":[59],"situ":[62],"hybridization":[63],"(ISH).":[64],"Using":[65],"double":[67],"cannulae":[68],"inserted":[69],"DVC,":[72],"(both":[76],"at":[77,101,108],"1x":[78],"10":[79,103],"9":[80],"cpu/ml,":[81],"200nl)":[82],"injected.":[84],"This":[85],"injection":[86],"method":[87],"allows":[88],"side":[91,93,148,166],"by":[92,140,169],"comparison":[94],"of":[95,209],"expression.":[97,158],"Brainstems":[98],"collected":[100],"day":[102],"after":[104],"treatment,":[105],"time":[107],"known":[112],"be":[114],"decreased.":[115],"ISH":[116],"performed":[118],"AT1a,":[121],"AT1b,":[123],"ACE1,":[124],"ACE2,":[126],"monitored":[127],"phosphor":[129],"imaging":[130],"autoradiography.":[132],"Results":[133],"showed:":[134],"decreased":[136],"61.2":[141],"\u00b1":[142],"6.8%":[143],"compared":[144],"with":[145],"(p<0.001).":[149],"had":[151,174],"no":[152,175],"effect":[153,176],"on":[154,162,177],"AT1b":[155],"receptor":[156,191],"ACE2":[159,198],"expression":[161],"treated":[165],"reduced":[168],"29.0\u00b114.5":[170],"%":[171],"(P<0.01).":[172],"ACE1.":[178],"These":[179],"results":[180],"demonstrate":[181],"that":[182],"can":[184],"site\u2010":[185],"subtype\u2010":[187],"specifically":[188],"silence":[189],"mRNA.":[192],"may":[194],"also":[195],"modulate":[196],"expression,":[199],"perhaps":[200],"reflecting":[201],"important":[202],"systems":[206],"blood":[210],"pressure":[211],"autonomic":[213],"function.":[214]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}