{"id":"https://openalex.org/W1823977813","doi":null,"title":"Hypoxia protects human corneal endothelium from tertiary butyl hydroperoxide and paraquat-induced cell death in vitro.","display_name":"Hypoxia protects human corneal endothelium from tertiary butyl hydroperoxide and paraquat-induced cell death in vitro.","publication_year":2007,"publication_date":"2007-03-01","ids":{"openalex":"https://openalex.org/W1823977813","mag":"1823977813","pmid":"https://pubmed.ncbi.nlm.nih.gov/17327479","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2643029"},"language":"en","primary_location":{"id":"pmid:17327479","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17327479","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental biology and medicine (Maywood, N.J.)","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2643029/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Qiang Cheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210119109","display_name":"Indiana University Bloomington","ror":"https://ror.org/02k40bc56","country_code":"US","type":"education","lineage":["https://openalex.org/I4210119109","https://openalex.org/I592451"]},{"id":"https://openalex.org/I592451","display_name":"Indiana University","ror":"https://ror.org/01kg8sb98","country_code":"US","type":"education","lineage":["https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Qiang Cheng","raw_affiliation_strings":["School of Optometry, Indiana University, Bloomington, IN 47405, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Optometry, Indiana University, Bloomington, IN 47405, USA","institution_ids":["https://openalex.org/I4210119109","https://openalex.org/I592451"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113795559","display_name":"Tracy T. Nguyen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tracy Nguyen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078445072","display_name":"Hongxin Song","orcid":"https://orcid.org/0000-0002-3653-6654","observed_orcids":["https://orcid.org/0000-0002-3653-6654","https://orcid.org/0000-0003-0325-3532"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hongxin Song","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5081947536","display_name":"Joseph A. Bonanno","orcid":"https://orcid.org/0000-0002-3842-7646","observed_orcids":["https://orcid.org/0000-0002-3842-7646"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Bonanno","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9494,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.75660802,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"232","issue":"3","first_page":"445","last_page":"53"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10563","display_name":"Corneal surgery and disorders","score":0.26440000534057617,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10563","display_name":"Corneal surgery and disorders","score":0.26440000534057617,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11828","display_name":"Corneal Surgery and Treatments","score":0.16940000653266907,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.10329999774694443,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hypoxia","display_name":"hypoxia","score":0.8429999947547913},{"id":"https://openalex.org/keywords/corneal-endothelium","display_name":"corneal endothelium","score":0.9620000123977661},{"id":"https://openalex.org/keywords/cell-death","display_name":"cell death","score":0.6830000281333923},{"id":"https://openalex.org/keywords/tertiary-butyl-hydroperoxide","display_name":"tertiary butyl hydroperoxide","score":0.9399999976158142},{"id":"https://openalex.org/keywords/paraquat","display_name":"paraquat","score":0.9990000128746033},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.9679999947547913},{"id":"https://openalex.org/keywords/mitochondrial-membrane-potential","display_name":"mitochondrial membrane potential","score":0.8450000286102295},{"id":"https://openalex.org/keywords/hypoxic-preconditioning","display_name":"hypoxic preconditioning","score":0.6380000114440918},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.45100000500679016}],"concepts":[{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.810970664024353},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.7373940348625183},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.6880135536193848},{"id":"https://openalex.org/C7836513","wikidata":"https://www.wikidata.org/wiki/Q1641506","display_name":"Hypoxia (environmental)","level":3,"score":0.6818759441375732},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.592960774898529},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5041016340255737},{"id":"https://openalex.org/C88634738","wikidata":"https://www.wikidata.org/wiki/Q24769543","display_name":"Annexin","level":3,"score":0.4532616138458252},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.44428551197052},{"id":"https://openalex.org/C2778589691","wikidata":"https://www.wikidata.org/wiki/Q3429577","display_name":"Rhodamine 123","level":4,"score":0.4417325258255005},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.41908693313598633},{"id":"https://openalex.org/C2776527163","wikidata":"https://www.wikidata.org/wiki/Q409669","display_name":"Paraquat","level":2,"score":0.4151993989944458},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4143100082874298},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4141855239868164},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.34785181283950806},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.33647477626800537},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2933271825313568},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.27250373363494873},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2568809688091278},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1449424922466278},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.0},{"id":"https://openalex.org/C133936738","wikidata":"https://www.wikidata.org/wiki/Q643839","display_name":"Multiple drug resistance","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004728","descriptor_name":"Endothelium, Corneal","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D004728","descriptor_name":"Endothelium, Corneal","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D004728","descriptor_name":"Endothelium, Corneal","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009707","descriptor_name":"Nucleosomes","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010269","descriptor_name":"Paraquat","qualifier_ui":"Q000633","qualifier_name":"toxicity","is_major_topic":true},{"descriptor_ui":"D015687","descriptor_name":"Cell Hypoxia","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D016923","descriptor_name":"Cell Death","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D016923","descriptor_name":"Cell Death","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D020122","descriptor_name":"tert-Butylhydroperoxide","qualifier_ui":"Q000633","qualifier_name":"toxicity","is_major_topic":true},{"descriptor_ui":"D042783","descriptor_name":"Endothelial Cells","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D042783","descriptor_name":"Endothelial Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D053078","descriptor_name":"Membrane Potential, Mitochondrial","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:17327479","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17327479","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental biology and medicine (Maywood, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2643029","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2643029/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Exp Biol Med (Maywood)","raw_type":"Text"},{"id":"mag:1823977813","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17327479","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2643029","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2643029/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Exp Biol Med (Maywood)","raw_type":"Text"},"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1327750461","display_name":"Regulation of pHi and Fluid Flux in Corneal Endothelium","funder_award_id":"R01EY008834","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G8432106566","display_name":"VISION RESEARCH AT INDIANA UNIVERSITY","funder_award_id":"R24EY016661","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W28004443","https://openalex.org/W1486527445","https://openalex.org/W1491901751","https://openalex.org/W1499316180","https://openalex.org/W1509592700","https://openalex.org/W1963904966","https://openalex.org/W1975416756","https://openalex.org/W1976881265","https://openalex.org/W1981013532","https://openalex.org/W1981936226","https://openalex.org/W1981940320","https://openalex.org/W1990189820","https://openalex.org/W1990958187","https://openalex.org/W2010855741","https://openalex.org/W2013643349","https://openalex.org/W2020316909","https://openalex.org/W2025060790","https://openalex.org/W2025455078","https://openalex.org/W2030319693","https://openalex.org/W2032478715","https://openalex.org/W2039598802","https://openalex.org/W2053804184","https://openalex.org/W2055456981","https://openalex.org/W2063110724","https://openalex.org/W2064271041","https://openalex.org/W2067977003","https://openalex.org/W2068428297","https://openalex.org/W2070732456","https://openalex.org/W2078532650","https://openalex.org/W2084609653","https://openalex.org/W2093099337","https://openalex.org/W2094985040","https://openalex.org/W2097294766","https://openalex.org/W2100248067","https://openalex.org/W2106745270","https://openalex.org/W2107739410","https://openalex.org/W2113999599","https://openalex.org/W2136137056","https://openalex.org/W2137383541","https://openalex.org/W2139576132","https://openalex.org/W2143718543","https://openalex.org/W2147929482","https://openalex.org/W2151767757","https://openalex.org/W2155018176","https://openalex.org/W2156462017","https://openalex.org/W2165347702","https://openalex.org/W2318560859","https://openalex.org/W4254283855"],"related_works":["https://openalex.org/W2766142871","https://openalex.org/W2025060790","https://openalex.org/W3113921339","https://openalex.org/W2097697539","https://openalex.org/W2076240373","https://openalex.org/W2800802884","https://openalex.org/W2086096373","https://openalex.org/W2437184925","https://openalex.org/W1973843131","https://openalex.org/W2021194772"],"abstract_inverted_index":{"To":[0],"develop":[1],"new":[2],"and":[3,8,33,56,70,76,96,100,113,123,134,139,188,210,237,247],"safe":[4],"approaches":[5],"to":[6,41,89,162,186,213],"protect":[7,42,228],"preserve":[9],"the":[10,13,36,170,191,215],"function":[11],"of":[12,25,67,115,132,205],"human":[14],"corneal":[15],"endothelium":[16],"(HCE),":[17],"we":[18],"have":[19,34],"established":[20],"an":[21,29],"in":[22,107,137,142,201],"vitro":[23],"model":[24],"cell":[26,31,97,105,206,232,245],"loss":[27],"using":[28],"HCE":[30,43,104,229],"line":[32],"examined":[35],"potential":[37,81],"for":[38],"hypoxia":[39,165,196,225,244],"conditioning":[40],"from":[44],"death":[45,60,106,207,233],"induced":[46,103,192],"by":[47,63,158,182,235],"two":[48],"ROS":[49],"generating":[50],"cytotoxins":[51],"(tertiary":[52],"butyl":[53],"hydroperoxide":[54],"[tBHP]":[55],"paraquat":[57],"[PQ]).":[58],"Cell":[59],"was":[61,83,250],"assessed":[62],"flow":[64],"cytometric":[65],"analysis":[66],"Annexin-V":[68],"(An)":[69],"propidium":[71],"iodide":[72],"(PI)":[73],"double":[74],"staining":[75],"oligonucleosome":[77],"production.":[78],"Mitochondrial":[79],"membrane":[80],"(MMP)":[82],"measured":[84],"with":[85,111,119],"JC-1":[86],"fluorescent":[87],"dye":[88],"determine":[90],"possible":[91],"associations":[92],"between":[93,243],"MMP":[94,193,217,248],"preservation":[95,249],"survival.":[98],"PQ":[99,135,185],"tBHP":[101,122,133,161,236],"both":[102],"a":[108,202],"dose-dependent":[109],"manner,":[110],"48%":[112],"32%":[114],"An(+)":[116,155,179],"cells":[117,156,180,230],"observed":[118],"60":[120,159],"microM":[121,125,160,184],"500":[124,183],"PQ,":[126,238],"respectively.":[127,144],"In":[128,219],"addition,":[129],"this":[130],"level":[131],"resulted":[136,200],"66%":[138],"49%":[140],"decreases":[141],"MMP,":[143],"Hypoxia":[145,173],"(0.6%":[146],"+/-":[147],"0.1%":[148],"oxygen)":[149],"pretreatment":[150,209],"(8":[151],"hrs)":[152,176,199],"significantly":[153],"reduced":[154,178],"caused":[157,181,234],"15%,":[163],"whereas":[164],"had":[166],"no":[167,240],"effect":[168],"on":[169],"decreased":[171],"MMP.":[172],"pretreatments":[174],"(24":[175,198],"slightly":[177],"25%":[187],"completely":[189],"prevented":[190],"reduction.":[194],"Conversely,":[195],"posttreatment":[197],"greater":[203],"inhibition":[204],"than":[208],"yet":[211],"failed":[212],"prevent":[214],"PQ-induced":[216],"collapse.":[218],"conclusion,":[220],"these":[221],"results":[222],"suggest":[223],"that":[224],"can":[226],"sufficiently":[227],"against":[231],"although":[239],"direct":[241],"link":[242],"protection":[246],"observed.":[251]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}