{"id":"https://openalex.org/W182346995","doi":"https://doi.org/10.1007/978-1-61779-998-3_9","title":"Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitative PCR","display_name":"Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitative PCR","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W182346995","doi":"https://doi.org/10.1007/978-1-61779-998-3_9","mag":"182346995","pmid":"https://pubmed.ncbi.nlm.nih.gov/22941600","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4422392"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-61779-998-3_9","doi":"https://doi.org/10.1007/978-1-61779-998-3_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-998-3_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W637943795","display_name":"DNA Repair Protocols"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4422392","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028109597","display_name":"Amy M. Furda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amy M. Furda","raw_affiliation_strings":["Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004684164","display_name":"Amanda S. Bess","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amanda Smith Bess","raw_affiliation_strings":["Nicholas School of the Environment, Duke University, Durham, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nicholas School of the Environment, Duke University, Durham, NC, USA","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015141183","display_name":"Joel N. Meyer","orcid":"https://orcid.org/0000-0003-1219-0983","observed_orcids":["https://orcid.org/0000-0003-1219-0983"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joel N. Meyer","raw_affiliation_strings":["Nicholas School of the Environment, Duke University, Durham, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nicholas School of the Environment, Duke University, Durham, NC, USA","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055919829","display_name":"Bennett Van Houten","orcid":"https://orcid.org/0000-0002-4009-2478","observed_orcids":["https://orcid.org/0000-0002-4009-2478"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]},{"id":"https://openalex.org/I4210128526","display_name":"UPMC Hillman Cancer Center","ror":"https://ror.org/03bw34a45","country_code":"US","type":"facility","lineage":["https://openalex.org/I170201317","https://openalex.org/I4210128526"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Bennett Van Houten","raw_affiliation_strings":["Department of Pharmacology and Chemical Biology, Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Chemical Biology, Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, USA","institution_ids":["https://openalex.org/I170201317","https://openalex.org/I4210128526"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5055919829"],"corresponding_institution_ids":["https://openalex.org/I170201317","https://openalex.org/I4210128526"],"apc_list":null,"apc_paid":null,"fwci":14.3173,"has_fulltext":false,"cited_by_count":101,"citation_normalized_percentile":{"value":0.99055763,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":100},"biblio":{"volume":"920","issue":null,"first_page":"111","last_page":"132"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.8657000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.8657000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.12770000100135803,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11359","display_name":"Effects of Radiation Exposure","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-damage","display_name":"DNA damage","score":0.8840000033378601},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.8949999809265137},{"id":"https://openalex.org/keywords/mitochondrial-dna-damage","display_name":"mitochondrial DNA damage","score":0.8029999732971191},{"id":"https://openalex.org/keywords/mitochondrial-dna-copy-number","display_name":"mitochondrial DNA copy number","score":0.9070000052452087},{"id":"https://openalex.org/keywords/nuclear-dna-damage","display_name":"nuclear DNA damage","score":0.7739999890327454},{"id":"https://openalex.org/keywords/caenorhabditis-elegans","display_name":"Caenorhabditis elegans","score":0.8659999966621399},{"id":"https://openalex.org/keywords/drosophila-melanogaster","display_name":"Drosophila melanogaster","score":1.0},{"id":"https://openalex.org/keywords/fundulus-heteroclitus","display_name":"Fundulus heteroclitus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/zebrafish","display_name":"zebrafish","score":0.9179999828338623},{"id":"https://openalex.org/keywords/animal-cell-culture","display_name":"animal cell culture","score":0.37599998712539673}],"concepts":[{"id":"https://openalex.org/C24586158","wikidata":"https://www.wikidata.org/wiki/Q27075","display_name":"Mitochondrial DNA","level":3,"score":0.7079789638519287},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7063087224960327},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6454272270202637},{"id":"https://openalex.org/C115960442","wikidata":"https://www.wikidata.org/wiki/Q769863","display_name":"Nuclear DNA","level":4,"score":0.579643964767456},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.5179694890975952},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.5086660981178284},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47272318601608276},{"id":"https://openalex.org/C2778944004","wikidata":"https://www.wikidata.org/wiki/Q91703","display_name":"Caenorhabditis elegans","level":3,"score":0.4720355272293091},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.40811413526535034},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3461494445800781},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22669166326522827}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004249","descriptor_name":"DNA Damage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011786","descriptor_name":"Quality Control","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1007/978-1-61779-998-3_9","doi":"https://doi.org/10.1007/978-1-61779-998-3_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-998-3_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:22941600","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22941600","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:3385524","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4422392","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4422392","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4422392","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:3385524","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4422392","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.504800021648407,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5299955960","display_name":"The Role of Mitochondrial DNA Damage in Neurodegeneration","funder_award_id":"R01ES017540","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W55405400","https://openalex.org/W1650754258","https://openalex.org/W1832212699","https://openalex.org/W1964357298","https://openalex.org/W1966940551","https://openalex.org/W1970810396","https://openalex.org/W1975369096","https://openalex.org/W1979369962","https://openalex.org/W1984803750","https://openalex.org/W1987010975","https://openalex.org/W1989681525","https://openalex.org/W1991030758","https://openalex.org/W1991883957","https://openalex.org/W1994113086","https://openalex.org/W1995435242","https://openalex.org/W2007697976","https://openalex.org/W2014903992","https://openalex.org/W2018719587","https://openalex.org/W2033064838","https://openalex.org/W2058108015","https://openalex.org/W2061083265","https://openalex.org/W2069611158","https://openalex.org/W2069736060","https://openalex.org/W2075247739","https://openalex.org/W2084586873","https://openalex.org/W2086154401","https://openalex.org/W2088084771","https://openalex.org/W2089257213","https://openalex.org/W2092969978","https://openalex.org/W2114243404","https://openalex.org/W2135272501","https://openalex.org/W2135426914","https://openalex.org/W2135700152","https://openalex.org/W2136905638","https://openalex.org/W2137018925","https://openalex.org/W2138268741","https://openalex.org/W2142596193","https://openalex.org/W2158780541","https://openalex.org/W4235271617"],"related_works":["https://openalex.org/W55405400","https://openalex.org/W2092969978","https://openalex.org/W2266621146","https://openalex.org/W2803478805","https://openalex.org/W1612859952","https://openalex.org/W2075247739","https://openalex.org/W2528248212","https://openalex.org/W2014903992","https://openalex.org/W2394856543","https://openalex.org/W2140952201"],"abstract_inverted_index":{"This":[0],"chapter":[1],"was":[2],"written":[3],"as":[4,23,25,29],"a":[5,181],"guide":[6],"to":[7,88,172],"using":[8,170],"the":[9,16,51,81,132,136,140,152,165,185],"long-amplicon":[10],"quantitative":[11],"PCR":[12],"(QPCR)":[13],"assay":[14,83,137,173,187],"for":[15,151],"measurement":[17,154],"of":[18,40,131,135,145,155,169,184],"DNA":[19,90,157,174],"damage":[20,91,175],"in":[21,50,95,176,192],"mammalian":[22],"well":[24],"nonmammalian":[26],"species":[27,39],"such":[28],"Caenorhabditis":[30],"elegans":[31],"(nematodes),":[32],"Drosophila":[33],"melanogaster":[34],"(fruit":[35],"flies),":[36],"and":[37,44,63,71,92,97,104,142,160,167,179],"two":[38],"fish":[41],"(Fundulus":[42],"heteroclitus":[43],"Danio":[45],"rerio).":[46],"Since":[47],"its":[48,190],"development":[49],"early":[52],"1990s":[53],"(Kalinowski":[54],"et":[55,115,123],"al.,":[56,116,124],"Nucleic":[57],"Acids":[58],"Res":[59,67,127],"20:3485-3494,":[60],"1992;":[61],"Salazar":[62],"Van":[64,72,105],"Houten,":[65,73,106],"Mutat":[66],"385:139-149,":[68],"1997;":[69,113],"Yakes":[70],"Proc":[74,107],"Natl":[75,108],"Acad":[76,109],"Sci":[77,110],"USA":[78,111],"94:514-519,":[79,112],"1997),":[80],"QPCR":[82,171,186],"has":[84],"been":[85],"widely":[86],"used":[87],"measure":[89],"repair":[93],"kinetics":[94],"nuclear":[96],"mitochondrial":[98,146,156],"genomes":[99],"after":[100],"genotoxin":[101],"exposure":[102],"(Yakes":[103],"Santos":[114],"J":[117],"Biol":[118],"Chem":[119],"278:1728-1734,":[120],"2003;":[121],"Mandavilli":[122],"Mol":[125],"Brain":[126],"133:215-223,":[128],"2005).":[129],"One":[130],"main":[133],"strengths":[134],"is":[138,148],"that":[139,188],"labor-intensive":[141],"artifact-generating":[143],"step":[144],"isolation":[147],"not":[149],"needed":[150],"accurate":[153],"copy":[158],"number":[159],"damage.":[161],"Below":[162],"we":[163],"present":[164],"advantages":[166],"limitations":[168],"animal":[177,195],"cells":[178],"provide":[180],"detailed":[182],"protocol":[183],"integrates":[189],"usage":[191],"newly":[193],"developed":[194],"systems.":[196]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":11},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":9},{"year":2016,"cited_by_count":10},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":14},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
