{"id":"https://openalex.org/W1787656802","doi":"https://doi.org/10.1042/bj3290579","title":"Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization","display_name":"Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization","publication_year":1998,"publication_date":"1998-02-01","ids":{"openalex":"https://openalex.org/W1787656802","doi":"https://doi.org/10.1042/bj3290579","mag":"1787656802","pmid":"https://pubmed.ncbi.nlm.nih.gov/9445385","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1219079"},"language":"en","primary_location":{"id":"doi:10.1042/bj3290579","doi":"https://doi.org/10.1042/bj3290579","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj3290579","pdf_url":"https://portlandpress.com/biochemj/article-pdf/329/3/579/630890/bj3290579.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/329/3/579/630890/bj3290579.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040239237","display_name":"Ran Taube","orcid":"https://orcid.org/0000-0002-2062-4537","observed_orcids":["https://orcid.org/0000-0002-2062-4537"]},"institutions":[{"id":"https://openalex.org/I16391192","display_name":"Tel Aviv University","ror":"https://ror.org/04mhzgx49","country_code":"IL","type":"education","lineage":["https://openalex.org/I16391192"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Ran TAUBE","raw_affiliation_strings":["Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel","institution_ids":["https://openalex.org/I16391192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113596473","display_name":"Shoshana Loya","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16391192","display_name":"Tel Aviv University","ror":"https://ror.org/04mhzgx49","country_code":"IL","type":"education","lineage":["https://openalex.org/I16391192"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Shoshana LOYA","raw_affiliation_strings":["Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel","institution_ids":["https://openalex.org/I16391192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009627802","display_name":"Orna Avidan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16391192","display_name":"Tel Aviv University","ror":"https://ror.org/04mhzgx49","country_code":"IL","type":"education","lineage":["https://openalex.org/I16391192"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Orna AVIDAN","raw_affiliation_strings":["Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel","institution_ids":["https://openalex.org/I16391192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062884138","display_name":"Michal Perach","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16391192","display_name":"Tel Aviv University","ror":"https://ror.org/04mhzgx49","country_code":"IL","type":"education","lineage":["https://openalex.org/I16391192"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Michal PERACH","raw_affiliation_strings":["Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel","institution_ids":["https://openalex.org/I16391192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049821570","display_name":"Amnon Hizi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16391192","display_name":"Tel Aviv University","ror":"https://ror.org/04mhzgx49","country_code":"IL","type":"education","lineage":["https://openalex.org/I16391192"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Amnon HIZI","raw_affiliation_strings":["Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel","institution_ids":["https://openalex.org/I16391192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I16391192"],"apc_list":null,"apc_paid":null,"fwci":2.2608,"has_fulltext":true,"cited_by_count":51,"citation_normalized_percentile":{"value":0.88209064,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"329","issue":"3","first_page":"579","last_page":"587"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10526","display_name":"HIV/AIDS drug development and treatment","score":0.8125,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10526","display_name":"HIV/AIDS drug development and treatment","score":0.8125,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.06379999965429306,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":0.030700000002980232,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mouse-mammary-tumor-virus","display_name":"mouse mammary tumor virus","score":0.9860000014305115},{"id":"https://openalex.org/keywords/reverse-transcriptase","display_name":"reverse transcriptase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/rnase-h-activity","display_name":"RNase H activity","score":0.6420000195503235},{"id":"https://openalex.org/keywords/dna-dependent-dna-polymerase","display_name":"DNA-dependent DNA polymerase","score":0.7490000128746033},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.4129999876022339}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6837332248687744},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6196524500846863},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.5910558700561523},{"id":"https://openalex.org/C131140672","wikidata":"https://www.wikidata.org/wiki/Q2031164","display_name":"Processivity","level":4,"score":0.5861085653305054},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.5799673795700073},{"id":"https://openalex.org/C2779777497","wikidata":"https://www.wikidata.org/wiki/Q1775519","display_name":"Mouse mammary tumor virus","level":3,"score":0.5593789219856262},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5507732629776001},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5139076709747314},{"id":"https://openalex.org/C199491388","wikidata":"https://www.wikidata.org/wiki/Q1869477","display_name":"Long terminal repeat","level":4,"score":0.49044105410575867},{"id":"https://openalex.org/C2910803801","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"RNA-Directed DNA Polymerase","level":5,"score":0.467792809009552},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4619915783405304},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4567834138870239},{"id":"https://openalex.org/C69766542","wikidata":"https://www.wikidata.org/wiki/Q3430179","display_name":"RNase H","level":5,"score":0.45433172583580017},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.4411817789077759},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.3524063527584076},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.16058146953582764},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1233205497264862}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002413","descriptor_name":"Cations, Divalent","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002458","descriptor_name":"Cell Fractionation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D004259","descriptor_name":"DNA-Directed DNA Polymerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008324","descriptor_name":"Mammary Tumor Virus, Mouse","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010443","descriptor_name":"Peptide Chain Termination, Translational","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D011189","descriptor_name":"Potassium Chloride","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012194","descriptor_name":"RNA-Directed DNA Polymerase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D012194","descriptor_name":"RNA-Directed DNA Polymerase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012194","descriptor_name":"RNA-Directed DNA Polymerase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012194","descriptor_name":"RNA-Directed DNA Polymerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012194","descriptor_name":"RNA-Directed DNA Polymerase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D016914","descriptor_name":"Ribonuclease H","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018894","descriptor_name":"Reverse Transcriptase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D019384","descriptor_name":"Nucleic Acid Synthesis Inhibitors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1042/bj3290579","doi":"https://doi.org/10.1042/bj3290579","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj3290579","pdf_url":"https://portlandpress.com/biochemj/article-pdf/329/3/579/630890/bj3290579.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1219079","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1219079","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:1219546","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1219546","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:9445385","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9445385","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"},{"id":"pmid:9622477","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9622477","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1042/bj3290579","doi":"https://doi.org/10.1042/bj3290579","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj3290579","pdf_url":"https://portlandpress.com/biochemj/article-pdf/329/3/579/630890/bj3290579.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1787656802.pdf","grobid_xml":"https://content.openalex.org/works/W1787656802.grobid-xml"},"referenced_works_count":56,"referenced_works":["https://openalex.org/W1484874812","https://openalex.org/W1492821293","https://openalex.org/W1493770836","https://openalex.org/W1495845650","https://openalex.org/W1504602313","https://openalex.org/W1531897207","https://openalex.org/W1543700786","https://openalex.org/W1549365118","https://openalex.org/W1581989954","https://openalex.org/W1582222346","https://openalex.org/W1584699951","https://openalex.org/W1600557458","https://openalex.org/W1624440538","https://openalex.org/W1632335268","https://openalex.org/W1738676508","https://openalex.org/W1787656802","https://openalex.org/W1801632833","https://openalex.org/W1888137339","https://openalex.org/W1895447826","https://openalex.org/W1903358508","https://openalex.org/W1965649387","https://openalex.org/W1970106134","https://openalex.org/W1980099789","https://openalex.org/W1983640004","https://openalex.org/W1988462426","https://openalex.org/W1989791734","https://openalex.org/W1991738184","https://openalex.org/W2000557169","https://openalex.org/W2001641409","https://openalex.org/W2008954911","https://openalex.org/W2009581957","https://openalex.org/W2027258995","https://openalex.org/W2029693258","https://openalex.org/W2036196796","https://openalex.org/W2038073731","https://openalex.org/W2043441365","https://openalex.org/W2058563055","https://openalex.org/W2059454895","https://openalex.org/W2066828278","https://openalex.org/W2081347685","https://openalex.org/W2089471930","https://openalex.org/W2091660763","https://openalex.org/W2093174110","https://openalex.org/W2094468496","https://openalex.org/W2128635872","https://openalex.org/W2148698673","https://openalex.org/W2166782710","https://openalex.org/W2210162472","https://openalex.org/W2323778292","https://openalex.org/W2344170192","https://openalex.org/W2398736662","https://openalex.org/W2415729395","https://openalex.org/W2549143424","https://openalex.org/W4206980014","https://openalex.org/W6600300406","https://openalex.org/W6638054014"],"related_works":["https://openalex.org/W2004130828","https://openalex.org/W2018283849","https://openalex.org/W2017471601","https://openalex.org/W1969683340","https://openalex.org/W2086739450","https://openalex.org/W2022938299","https://openalex.org/W2013246668","https://openalex.org/W4283165997","https://openalex.org/W2148263450","https://openalex.org/W3216602089"],"abstract_inverted_index":{"We":[0],"have":[1,192],"constructed":[2],"a":[3,24,27,84,92,114,119,147,184,189,220,261],"plasmid":[4],"that":[5,249,268],"induces":[6],"in":[7,49,77],"bacteria":[8],"the":[9,42,45,50,53,63,70,106,126,206,211,242,256],"synthesis":[10,265],"of":[11,18,44,52,144,156,163,167,176,198,205,209,214,234,250,263,269],"an":[12,57],"enzymically":[13,111],"active":[14,112],"reverse":[15],"transcriptase":[16],"(RT)":[17],"mouse":[19],"mammary":[20],"tumour":[21],"virus":[22],"(MMTV),":[23],"retrovirus":[25],"with":[26,83,203],"typical":[28],"B-type":[29],"morphology.":[30],"The":[31,80,195],"highest":[32],"catalytic":[33,196],"activity":[34],"was":[35,60,87],"detected":[36],"only":[37],"when":[38],"27":[39],"residues":[40],"from":[41],"C-terminus":[43],"protease":[46],"were":[47,201],"included":[48],"N-terminus":[51],"recombinant":[54,81,127,257],"RT,":[55],"after":[56,117],"extra":[58],"deoxyadenosine":[59],"added":[61],"between":[62],"pro":[64],"and":[65,132,181,187,237,252],"pol":[66],"genes":[67],"to":[68,89,183,188,223,230],"overcome":[69],"-1":[71],"frameshift":[72],"event":[73],"(which":[74,227],"occurs":[75],"naturally":[76],"virus-infected":[78],"cells).":[79],"protein":[82],"six-histidine":[85],"tag":[86],"purified":[88,107],"homogeneity":[90],"by":[91,100],"two-column":[93],"purification":[94],"procedure,":[95],"Ni2+":[96],"nitriloacetic":[97],"acid/agarose":[98],"followed":[99],"carboxymethyl-Sepharose":[101],"chromatography.":[102],"Unlike":[103],"most":[104],"RTs,":[105],"MMTV":[108,128,199,217,258],"RT":[109,129,200,208,218,259],"is":[110],"as":[113,137,139,161,241,246,248],"monomer":[115],"even":[116],"binding":[118],"DNA":[120,134,164,168,264],"substrate.":[121],"Like":[122],"all":[123,143],"RTs":[124,236],"studied,":[125],"possesses":[130],"RNA-dependent":[131],"DNA-dependent":[133],"polymerase":[135],"activities":[136],"well":[138],"RNase":[140],"H":[141],"activity,":[142],"which":[145],"show":[146],"preference":[148],"for":[149],"Mg2+":[150],"over":[151],"Mn2+":[152],"ions.":[153],"Other":[154],"features":[155],"these":[157],"enzymic":[158],"activities,":[159],"such":[160],"extension":[162],"primers,":[165],"processivity":[166,262],"synthesis,":[169],"pH":[170],"dependence,":[171],"steady-state":[172],"kinetic":[173],"constants,":[174],"effects":[175],"Na+":[177],"or":[178],"K+":[179],"ions":[180],"sensitivity":[182,222],"thiol-specific":[185],"reagent":[186],"zinc":[190],"chelator,":[191],"been":[193],"evaluated.":[194],"properties":[197],"compared":[202],"those":[204],"well-studied":[207],"HIV-1,":[210],"causative":[212],"agent":[213],"AIDS.":[215],"Interestingly,":[216],"exhibits":[219],"high":[221,247],"nucleoside":[224],"triphosphate":[225],"analogues":[226],"are":[228,238],"known":[229],"be":[231],"potent":[232],"inhibitors":[233],"HIV":[235],"being":[239],"used":[240],"major":[243],"anti-AIDS":[244],"drugs),":[245],"HIV-1":[251,270],"HIV-2":[253],"RTs.":[254],"Furthermore":[255],"shows":[260],"higher":[266],"than":[267],"RT.":[271]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}