{"id":"https://openalex.org/W178762106","doi":null,"title":"Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency.","display_name":"Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency.","publication_year":2000,"publication_date":"2000-04-01","ids":{"openalex":"https://openalex.org/W178762106","mag":"178762106","pmid":"https://pubmed.ncbi.nlm.nih.gov/10805248","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1189592"},"language":"en","primary_location":{"id":"pmh:oai:europepmc.org:86365","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC1189592","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC1189592","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"C Simard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"C Simard","raw_affiliation_strings":["Retrovirology Centre of Expertise, Canadian Food Inspection Agency, Charlottetown, Prince Edward Island. simardc@em.agr.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Retrovirology Centre of Expertise, Canadian Food Inspection Agency, Charlottetown, Prince Edward Island. simardc@em.agr.ca","institution_ids":["https://openalex.org/I1307613440"]}]},{"author_position":"middle","author":{"id":null,"display_name":"S Richardson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Richardson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"P Dixon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Dixon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"C B\u00e9langer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C B\u00e9langer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"P Maxwell","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Maxwell","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4445,"has_fulltext":false,"cited_by_count":44,"citation_normalized_percentile":{"value":0.58208545,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"64","issue":"2","first_page":"101","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.004600000102072954,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-leukosis","display_name":"bovine leukosis","score":0.9909999966621399},{"id":"https://openalex.org/keywords/bovine-leukemia-virus","display_name":"bovine leukemia virus","score":0.9980000257492065},{"id":"https://openalex.org/keywords/elisa","display_name":"ELISA","score":0.7540000081062317},{"id":"https://openalex.org/keywords/agar-gel-immunodiffusion","display_name":"agar gel immunodiffusion","score":0.8980000019073486},{"id":"https://openalex.org/keywords/serological-diagnosis","display_name":"serological diagnosis","score":0.8820000290870667},{"id":"https://openalex.org/keywords/p24-core-protein","display_name":"p24 core protein","score":0.9980000257492065},{"id":"https://openalex.org/keywords/diagnostic-test-comparison","display_name":"diagnostic test comparison","score":0.6340000033378601},{"id":"https://openalex.org/keywords/cattle","display_name":"cattle","score":0.39899998903274536},{"id":"https://openalex.org/keywords/antibody-detection-sensitivity","display_name":"antibody detection sensitivity","score":0.6010000109672546}],"concepts":[{"id":"https://openalex.org/C80020023","wikidata":"https://www.wikidata.org/wiki/Q12730368","display_name":"Ouchterlony double immunodiffusion","level":4,"score":0.8642722368240356},{"id":"https://openalex.org/C2778645089","wikidata":"https://www.wikidata.org/wiki/Q895365","display_name":"Bovine leukemia virus","level":3,"score":0.7650289535522461},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5749682188034058},{"id":"https://openalex.org/C196955773","wikidata":"https://www.wikidata.org/wiki/Q256632","display_name":"Immunodiffusion","level":3,"score":0.5622396469116211},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5226404666900635},{"id":"https://openalex.org/C184112429","wikidata":"https://www.wikidata.org/wiki/Q476174","display_name":"Serial dilution","level":3,"score":0.500361442565918},{"id":"https://openalex.org/C45189115","wikidata":"https://www.wikidata.org/wiki/Q502159","display_name":"Serology","level":3,"score":0.4797281324863434},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3548460602760315},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3531523644924164},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.351076602935791},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.2557453513145447},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.22077476978302002},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1557619273662567},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000362","descriptor_name":"Agar","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001909","descriptor_name":"Leukemia Virus, Bovine","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002418","descriptor_name":"Cattle Diseases","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D002418","descriptor_name":"Cattle Diseases","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false},{"descriptor_ui":"D005779","descriptor_name":"Immunodiffusion","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005779","descriptor_name":"Immunodiffusion","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012698","descriptor_name":"Serologic Tests","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016583","descriptor_name":"Enzootic Bovine Leukosis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D016583","descriptor_name":"Enzootic Bovine Leukosis","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false}],"locations_count":4,"locations":[{"id":"pmh:oai:europepmc.org:86365","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC1189592","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:1189592","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1189592","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Can J Vet Res","raw_type":"Text"},{"id":"pmid:10805248","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10805248","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","raw_type":"Comparative Study"},{"id":"mag:178762106","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10805248","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:europepmc.org:86365","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC1189592","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[{"score":0.5917999744415283,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W244770800","https://openalex.org/W842394465","https://openalex.org/W1441754332","https://openalex.org/W1503032535","https://openalex.org/W1510678933","https://openalex.org/W1525207864","https://openalex.org/W1893667475","https://openalex.org/W1987280988","https://openalex.org/W2013993948","https://openalex.org/W2015624892","https://openalex.org/W2017121001","https://openalex.org/W2023474849","https://openalex.org/W2026170025","https://openalex.org/W2031632680","https://openalex.org/W2049155667","https://openalex.org/W2068009655","https://openalex.org/W2093768446","https://openalex.org/W2128270376","https://openalex.org/W2139053144","https://openalex.org/W2144770343","https://openalex.org/W2160387407","https://openalex.org/W2302284150","https://openalex.org/W2411691225","https://openalex.org/W2416663885","https://openalex.org/W2432806393"],"related_works":["https://openalex.org/W191780380","https://openalex.org/W2416663885","https://openalex.org/W1973600217","https://openalex.org/W2003065714","https://openalex.org/W2132515530","https://openalex.org/W2319653517","https://openalex.org/W2068009655","https://openalex.org/W1798769933","https://openalex.org/W1503032535","https://openalex.org/W4361861219"],"abstract_inverted_index":{"Four":[0],"commercially":[1],"available":[2],"bovine":[3,187],"leukemia":[4],"virus":[5],"(BLV)-ELISA":[6],"kits":[7,43,69,119],"from":[8],"Europe":[9],"or":[10],"the":[11,17,26,46,55,67,80,91,113,116,127,154,173,184],"United":[12],"States":[13],"were":[14,39,61,109,120,132],"compared":[15],"to":[16,86,122,141,167],"agar":[18],"gel":[19],"immunodiffusion":[20],"(AGID)":[21],"test":[22,95,129,181],"officially":[23,92],"approved":[24,93],"by":[25],"Canadian":[27],"Food":[28],"Inspection":[29],"Agency":[30],"(CFIA).":[31],"A":[32],"total":[33],"of":[34,99,134,160,186],"1200":[35],"cattle":[36],"serum":[37,107],"samples":[38,108,138,164],"used.":[40],"Three":[41],"ELISA":[42,68,76,118],"based":[44,78],"on":[45,79,112,170],"envelope":[47],"glycoprotein":[48],"(gp51)":[49],"gave":[50],"an":[51,179],"excellent":[52],"correlation":[53],"with":[54],"AGID":[56,94,104,128,155],"test.":[57],"The":[58,75],"kappa":[59],"values":[60],"0.998,":[62],"0.984,":[63],"and":[64,72,96,149],"0.986":[65],"for":[66,183],"#1,":[70],"#2,":[71],"#3,":[73],"respectively.":[74],"kit":[77,156],"p24":[81],"core":[82],"protein":[83],"was":[84,157,176],"found":[85,121],"be":[87,123],"less":[88],"sensitive":[89,125],"than":[90,126],"detected":[97],"5.13%":[98],"false":[100],"negatives.":[101],"Forty":[102],"BLV":[103],"strongly":[105],"positive":[106],"diluted.":[110],"Based":[111,169],"dilution":[114],"experiment,":[115],"gp51":[117,174],"more":[124],"kits.":[130],"They":[131],"capable":[133,159],"detecting":[135,161],"antibodies":[136,162],"in":[137,163,189],"diluted":[139,165],"up":[140,166],"1/5000":[142],"(kit":[143,147,151],"#1),":[144],"1/20":[145],"800":[146],"#2)":[148],"1/4000":[150],"#3),":[152],"whereas":[153],"only":[158],"1/100.":[168],"these":[171],"observations,":[172],"BLV-ELISA":[175],"recognized":[177],"as":[178],"official":[180],"method":[182],"serodiagnosis":[185],"leukosis":[188],"Canada.":[190]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}