{"id":"https://openalex.org/W1776350144","doi":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","title":"Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolation of strain-specific Rhizobium DNA sequences","display_name":"Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolation of strain-specific Rhizobium DNA sequences","publication_year":1992,"publication_date":"1992-07-01","ids":{"openalex":"https://openalex.org/W1776350144","doi":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","mag":"1776350144","pmid":"https://pubmed.ncbi.nlm.nih.gov/1637166","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/195771"},"language":"en","primary_location":{"id":"doi:10.1128/aem.58.7.2296-2301.1992","doi":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","pdf_url":"https://aem.asm.org/content/aem/58/7/2296.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://aem.asm.org/content/aem/58/7/2296.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026962542","display_name":"Anthony J. Bjourson","orcid":"https://orcid.org/0000-0002-1105-7147","observed_orcids":["https://orcid.org/0000-0002-1105-7147"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A J Bjourson","raw_affiliation_strings":["Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Belfast"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Belfast","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111868829","display_name":"C. Stone","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C E Stone","raw_affiliation_strings":["Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Belfast"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Belfast","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103702050","display_name":"James E Cooper","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J E Cooper","raw_affiliation_strings":["Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Belfast"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Belfast","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.9245,"has_fulltext":false,"cited_by_count":55,"citation_normalized_percentile":{"value":0.9663789,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"58","issue":"7","first_page":"2296","last_page":"2301"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11470","display_name":"Legume Nitrogen Fixing Symbiosis","score":0.9067000150680542,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11470","display_name":"Legume Nitrogen Fixing Symbiosis","score":0.9067000150680542,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.03920000046491623,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.02319999970495701,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rhizobium-leguminosarum-bv-trifolii","display_name":"Rhizobium leguminosarum bv. trifolii","score":0.9980000257492065},{"id":"https://openalex.org/keywords/uracil-dna-glycosylase","display_name":"uracil-DNA glycosylase","score":0.9480000138282776},{"id":"https://openalex.org/keywords/hybridization-specificity","display_name":"hybridization specificity","score":0.5550000071525574},{"id":"https://openalex.org/keywords/bacterial-genomics","display_name":"bacterial genomics","score":0.3269999921321869}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6224355697631836},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5968354940414429},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.583267331123352},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5168136358261108},{"id":"https://openalex.org/C52001828","wikidata":"https://www.wikidata.org/wiki/Q2902493","display_name":"Primer dimer","level":5,"score":0.499359130859375},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.4714829921722412},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.45148852467536926},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.4258957505226135},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26012781262397766},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.23655551671981812},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.23521903157234192},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09925833344459534},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005784","descriptor_name":"Gene Amplification","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016961","descriptor_name":"Rhizobium leguminosarum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.58.7.2296-2301.1992","doi":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","pdf_url":"https://aem.asm.org/content/aem/58/7/2296.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:88655","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC195771","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:195771","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/195771","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},{"id":"pmid:1637166","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1637166","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1128/aem.58.7.2296-2301.1992","doi":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.58.7.2296-2301.1992","pdf_url":"https://aem.asm.org/content/aem/58/7/2296.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1776350144.pdf"},"referenced_works_count":13,"referenced_works":["https://openalex.org/W1937050425","https://openalex.org/W1963668220","https://openalex.org/W1970716023","https://openalex.org/W1971026904","https://openalex.org/W1974119162","https://openalex.org/W1996391238","https://openalex.org/W1997710054","https://openalex.org/W2016481229","https://openalex.org/W2016740801","https://openalex.org/W2030397711","https://openalex.org/W2096611980","https://openalex.org/W2116346773","https://openalex.org/W2144634347"],"related_works":["https://openalex.org/W2281216608","https://openalex.org/W1937050425","https://openalex.org/W179821032","https://openalex.org/W1505770969","https://openalex.org/W2079653867","https://openalex.org/W2400315286","https://openalex.org/W2001776107","https://openalex.org/W1494732622","https://openalex.org/W2077641874","https://openalex.org/W141270020"],"abstract_inverted_index":{"A":[0],"novel":[1],"subtraction":[2],"hybridization":[3],"procedure,":[4],"incorporating":[5],"a":[6,20,39,66,91,140,180],"combination":[7],"of":[8,22,48,56,90,96,118,169,196],"four":[9],"separation":[10],"strategies,":[11],"was":[12,36],"developed":[13],"to":[14,38,65,77,173,178,193],"isolate":[15],"unique":[16],"DNA":[17,28,51,81,107,121,128,159,197],"sequences":[18,98,132,176,200],"from":[19,29,52,160,201],"strain":[21,162],"Rhizobium":[23],"leguminosarum":[24],"bv.":[25],"trifolii.":[26],"Sau3A-digested":[27],"this":[30],"strain,":[31,35],"i.e.,":[32],"the":[33,57,97,101,164,194],"probe":[34,141,181],"ligated":[37,64],"linker":[40],"and":[41,71,82,147,177,198,204],"hybridized":[42,183],"in":[43,100,151,163],"solution":[44],"with":[45,126,139,145,185],"an":[46],"excess":[47],"pooled":[49],"subtracter":[50,106,120,165],"seven":[53],"other":[54],"strains":[55],"same":[58],"biovar":[59],"which":[60,108,182],"had":[61],"been":[62],"restricted,":[63],"different,":[67],"biotinylated,":[68],"subtracter-specific":[69],"linker,":[70],"amplified":[72,134],"by":[73,87,113,124,135],"polymerase":[74,136],"chain":[75,137],"reaction":[76,138],"incorporate":[78],"dUTP.":[79],"Subtracter":[80],"subtracter-probe":[83],"hybrids":[84],"were":[85,122,133,171],"removed":[86,123],"phenol-chloroform":[88,114],"extraction":[89],"streptavidin-biotin-DNA":[92],"complex.":[93],"NENSORB":[94],"chromatography":[95],"remaining":[99,131],"aqueous":[102],"layer":[103],"captured":[104],"biotinylated":[105],"may":[109],"have":[110],"escaped":[111],"removal":[112],"treatment.":[115],"Any":[116],"traces":[117],"contaminating":[119],"digestion":[125],"uracil":[127],"glycosylase.":[129],"Finally,":[130],"strain-specific":[142],"primer,":[143],"labelled":[144],"32P,":[146],"tested":[148],"for":[149],"specificity":[150],"dot":[152],"blot":[153],"hybridizations":[154],"against":[155],"total":[156],"genomic":[157],"target":[158,187],"each":[161],"pool.":[166],"Two":[167],"rounds":[168],"subtraction-amplification":[170],"sufficient":[172],"remove":[174],"cross-hybridizing":[175],"give":[179],"only":[184],"homologous":[186],"DNA.":[188],"The":[189],"method":[190],"is":[191],"applicable":[192],"isolation":[195],"RNA":[199],"both":[202],"procaryotic":[203],"eucaryotic":[205],"cells.":[206]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}