{"id":"https://openalex.org/W176996418","doi":"https://doi.org/10.1021/ja043900e","title":"Characterization of tylM3/tylM2 and mydC/mycB Pairs Required for Efficient Glycosyltransfer in Macrolide Antibiotic Biosynthesis","display_name":"Characterization of tylM3/tylM2 and mydC/mycB Pairs Required for Efficient Glycosyltransfer in Macrolide Antibiotic Biosynthesis","publication_year":2004,"publication_date":"2004-12-01","ids":{"openalex":"https://openalex.org/W176996418","doi":"https://doi.org/10.1021/ja043900e","mag":"176996418","pmid":"https://pubmed.ncbi.nlm.nih.gov/15612702"},"language":"he","primary_location":{"id":"doi:10.1021/ja043900e","doi":"https://doi.org/10.1021/ja043900e","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja043900e","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051788322","display_name":"Charles E. Melan\u00e7on","orcid":"https://orcid.org/0000-0002-4236-3868","observed_orcids":["https://orcid.org/0000-0002-4236-3868"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles E. Melan\u00e7on","raw_affiliation_strings":["Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073248392","display_name":"Haruko Takahashi","orcid":"https://orcid.org/0000-0002-8607-796X","observed_orcids":["https://orcid.org/0000-0002-8607-796X"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haruko Takahashi","raw_affiliation_strings":["Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084628633","display_name":"Hung\u2010wen Liu","orcid":"https://orcid.org/0000-0001-8953-4794","observed_orcids":["https://orcid.org/0000-0001-8953-4794"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hung-wen Liu","raw_affiliation_strings":["Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.933,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.89408411,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"126","issue":"51","first_page":"16726","last_page":"16727"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/macrolide-biosynthesis","display_name":"macrolide synthesis","score":0.8019999861717224},{"id":"https://openalex.org/keywords/glycosyltransferases","display_name":"glycosyltransferases","score":0.7960000038146973},{"id":"https://openalex.org/keywords/streptomyces-venezuelae","display_name":"Streptomyces venezuelae","score":0.9039999842643738},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.871999979019165},{"id":"https://openalex.org/keywords/anthracycline-biosynthesis","display_name":"anthracycline biosynthesis","score":0.8339999914169312},{"id":"https://openalex.org/keywords/natural-product-biosynthesis","display_name":"natural product biosynthesis","score":0.6079999804496765}],"concepts":[{"id":"https://openalex.org/C161019410","wikidata":"https://www.wikidata.org/wiki/Q67201373","display_name":"Glycosyltransferase","level":3,"score":0.949015736579895},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7635865211486816},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6762078404426575},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.5668396949768066},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.5340648293495178},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.5228232145309448},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.485245943069458},{"id":"https://openalex.org/C2779920670","wikidata":"https://www.wikidata.org/wiki/Q1144013","display_name":"Streptomyces","level":3,"score":0.4777785539627075},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4705263376235962},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3284044861793518},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2402067482471466},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.20542612671852112},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.14619126915931702},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13474449515342712}],"mesh":[{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D008847","descriptor_name":"Micromonospora","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008847","descriptor_name":"Micromonospora","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008847","descriptor_name":"Micromonospora","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016695","descriptor_name":"Glycosyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016695","descriptor_name":"Glycosyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016930","descriptor_name":"Saccharopolyspora","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D016930","descriptor_name":"Saccharopolyspora","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016930","descriptor_name":"Saccharopolyspora","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018942","descriptor_name":"Macrolides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/ja043900e","doi":"https://doi.org/10.1021/ja043900e","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja043900e","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:15612702","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15612702","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1876139105","display_name":"C-C & C-N Bond Formation in Unusual Sugar Biosynthesis","funder_award_id":"R01GM054346","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G2802058731","display_name":null,"funder_award_id":"GM 54346","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G2973449470","display_name":null,"funder_award_id":"GM35906","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W1964271204","https://openalex.org/W1995995599","https://openalex.org/W2008030704","https://openalex.org/W2048848065","https://openalex.org/W2092327186","https://openalex.org/W2101967572"],"related_works":["https://openalex.org/W2043590759","https://openalex.org/W1936237592","https://openalex.org/W2048063010","https://openalex.org/W2071939251","https://openalex.org/W2048848065","https://openalex.org/W2395927655","https://openalex.org/W2124106662","https://openalex.org/W2260539383","https://openalex.org/W2072717287","https://openalex.org/W2151509947"],"abstract_inverted_index":{"The":[0],"heterologous":[1],"expression":[2,73],"of":[3,10],"tylM3":[4],"and":[5,23,25,88],"mydC,":[6],"two":[7],"homologous":[8],"genes":[9,16,30],"previously":[11],"unknown":[12],"function,":[13],"along":[14],"with":[15],"encoding":[17],"their":[18],"respective":[19],"partner":[20],"glycosyltransferases,":[21],"tylM2":[22],"mycB,":[24],"the":[26,33,37,44,54,60,79],"necessary":[27],"sugar":[28],"biosynthesis":[29],"significantly":[31],"enhances":[32],"glycosyltransferase":[34,95],"activity":[35],"in":[36,42,86,111,114],"engineered":[38],"Streptomyces":[39],"venezuelae":[40],"host":[41],"which":[43],"native":[45],"glycosyltransferase,":[46],"desVII,":[47],"has":[48],"been":[49],"inactivated.":[50],"Both":[51],"glycosyltransferases":[52],"accept":[53],"endogenous":[55],"12-membered":[56],"macrolide,":[57,64],"10-deoxymethynolide,":[58],"or":[59],"exogenously":[61],"fed":[62],"16-membered":[63],"tylactone.":[65],"Five":[66],"new":[67,108],"compounds":[68],"were":[69],"generated":[70],"using":[71],"this":[72],"system.":[74],"This":[75],"work":[76],"suggests":[77],"that":[78],"13":[80],"other":[81],"known":[82],"TylM3/MydC/DesVIII":[83],"homologues":[84],"found":[85],"macrolide":[87],"anthracycline":[89],"antibiotic":[90],"clusters":[91],"likely":[92],"function":[93],"as":[94,98],"auxiliary":[96],"proteins":[97],"well.":[99],"These":[100],"findings":[101],"will":[102],"greatly":[103],"assist":[104],"endeavors":[105],"to":[106],"generate":[107],"natural":[109],"products":[110],"these":[112],"pathways":[113],"a":[115],"combinatorial":[116],"fashion.":[117]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}