{"id":"https://openalex.org/W1769319829","doi":"https://doi.org/10.1007/978-1-62703-992-5_11","title":"Human B Cell Immortalization for Monoclonal Antibody Production","display_name":"Human B Cell Immortalization for Monoclonal Antibody Production","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W1769319829","doi":"https://doi.org/10.1007/978-1-62703-992-5_11","mag":"1769319829","pmid":"https://pubmed.ncbi.nlm.nih.gov/24515466"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-62703-992-5_11","doi":"https://doi.org/10.1007/978-1-62703-992-5_11","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-992-5_11","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4253160580","display_name":"Monoclonal Antibodies"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085391088","display_name":"Joyce S Hui\u2010Yuen","orcid":"https://orcid.org/0000-0002-0545-906X","observed_orcids":["https://orcid.org/0000-0002-0545-906X"]},"institutions":[{"id":"https://openalex.org/I4210087560","display_name":"Stony Brook School","ror":"https://ror.org/003wx9n05","country_code":"US","type":"education","lineage":["https://openalex.org/I4210087560"]},{"id":"https://openalex.org/I4210102711","display_name":"Stony Brook Medicine","ror":"https://ror.org/01882y777","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210102711","https://openalex.org/I59553526"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joyce Hui-Yuen","raw_affiliation_strings":["Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I4210087560","https://openalex.org/I4210102711","https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110007035","display_name":"Siva Rama Krishna Koganti","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210087560","display_name":"Stony Brook School","ror":"https://ror.org/003wx9n05","country_code":"US","type":"education","lineage":["https://openalex.org/I4210087560"]},{"id":"https://openalex.org/I4210102711","display_name":"Stony Brook Medicine","ror":"https://ror.org/01882y777","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210102711","https://openalex.org/I59553526"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Siva Koganti","raw_affiliation_strings":["Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I4210087560","https://openalex.org/I4210102711","https://openalex.org/I59553526"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015173460","display_name":"Sumita Bhaduri\u2010McIntosh","orcid":"https://orcid.org/0000-0003-2946-9497","observed_orcids":["https://orcid.org/0000-0003-2946-9497"]},"institutions":[{"id":"https://openalex.org/I4210087560","display_name":"Stony Brook School","ror":"https://ror.org/003wx9n05","country_code":"US","type":"education","lineage":["https://openalex.org/I4210087560"]},{"id":"https://openalex.org/I4210102711","display_name":"Stony Brook Medicine","ror":"https://ror.org/01882y777","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210102711","https://openalex.org/I59553526"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sumita Bhaduri-McIntosh","raw_affiliation_strings":["Department of Molecular Genetics and Microbiology, Stony Brook University School of Medicine, Stony Brook, NY, USA","Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Genetics and Microbiology, Stony Brook University School of Medicine, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I59553526"]},{"raw_affiliation_string":"Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I4210087560","https://openalex.org/I4210102711","https://openalex.org/I59553526"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.9524,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.91153295,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"1131","issue":null,"first_page":"183","last_page":"189"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7577999830245972,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7577999830245972,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":0.14869999885559082,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.027300000190734863,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/b-cell-immortalization","display_name":"B cell immortalization","score":0.8700000047683716},{"id":"https://openalex.org/keywords/epstein-barr-virus","display_name":"Epstein-Barr virus","score":0.8740000128746033},{"id":"https://openalex.org/keywords/lymphoblastoid-cell-lines","display_name":"lymphoblastoid cell lines","score":0.984000027179718},{"id":"https://openalex.org/keywords/monoclonal-antibody-production","display_name":"monoclonal antibody production","score":0.8790000081062317},{"id":"https://openalex.org/keywords/peripheral-blood","display_name":"peripheral blood","score":0.5989999771118164},{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.7670000195503235},{"id":"https://openalex.org/keywords/antigen-presentation","display_name":"antigen presentation","score":0.8769999742507935}],"concepts":[{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.8529062867164612},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6210966110229492},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5687568783760071},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5473624467849731},{"id":"https://openalex.org/C113842279","wikidata":"https://www.wikidata.org/wiki/Q1873857","display_name":"Lymphoblast","level":3,"score":0.5465517044067383},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5344812870025635},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5120120644569397},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5051172375679016},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4234750270843506},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.42257189750671387},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3755865693092346},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07595205307006836}],"mesh":[{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001402","descriptor_name":"B-Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001402","descriptor_name":"B-Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001402","descriptor_name":"B-Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-62703-992-5_11","doi":"https://doi.org/10.1007/978-1-62703-992-5_11","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-992-5_11","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:24515466","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24515466","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5901681335","display_name":null,"funder_award_id":"K12HD001401","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G8052269359","display_name":"Yale University Clinical and Translational Science Award Program","funder_award_id":"UL1RR024139","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G8494880723","display_name":"Early cellular immune responses to EBV","funder_award_id":"K08AI062732","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1979401674","https://openalex.org/W1998383538","https://openalex.org/W2018716777","https://openalex.org/W2039515480","https://openalex.org/W2061654110","https://openalex.org/W2065327430","https://openalex.org/W2067477865","https://openalex.org/W2079910001","https://openalex.org/W2116458521","https://openalex.org/W2119231028","https://openalex.org/W2129738847","https://openalex.org/W2180633678","https://openalex.org/W2207636101"],"related_works":["https://openalex.org/W2073601989","https://openalex.org/W2042208882","https://openalex.org/W1497704670","https://openalex.org/W2375216456","https://openalex.org/W2085444160","https://openalex.org/W2160559213","https://openalex.org/W2415105881","https://openalex.org/W2092159986","https://openalex.org/W2947566581","https://openalex.org/W1966371995"],"abstract_inverted_index":{"Infection":[0],"of":[1,27,35],"primary":[2],"B":[3],"lymphocytes":[4],"with":[5,56],"Epstein-Barr":[6],"virus":[7],"gives":[8],"rise":[9],"to":[10,29,39,49],"growth-transformed":[11],"and":[12,38,59],"immortalized":[13],"lymphoblastoid":[14],"cell":[15],"lines":[16],"(LCL)":[17],"in":[18,32],"vitro.":[19],"Among":[20],"their":[21],"many":[22],"applications":[23],"is":[24],"the":[25],"use":[26],"LCL":[28,51],"present":[30],"antigens":[31],"a":[33,47],"variety":[34],"immunologic":[36],"assays":[37],"generate":[40,50],"human":[41],"monoclonal":[42],"antibodies.":[43],"This":[44],"chapter":[45],"describes":[46],"method":[48],"from":[52],"donor":[53],"peripheral":[54],"blood":[55],"rapid":[57],"immortalization":[58],"cryopreservation":[60],"times.":[61]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-15T10:00:46.086208","created_date":"2025-10-10T00:00:00"}