{"id":"https://openalex.org/W176884152","doi":null,"title":"Protective effect of caffeine against high sugar-induced transcription of microRNAs and consequent gene silencing: a study using lenses of galactosemic mice.","display_name":"Protective effect of caffeine against high sugar-induced transcription of microRNAs and consequent gene silencing: a study using lenses of galactosemic mice.","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W176884152","mag":"176884152","pmid":"https://pubmed.ncbi.nlm.nih.gov/23441122","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3580991"},"language":"en","primary_location":{"id":"pmh:oai:europepmc.org:2578863","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC3580991","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/articles/PMC3580991","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111400173","display_name":"Shambhu D. Varma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shambhu D Varma","raw_affiliation_strings":["Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA. svarm001@umaryland.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA. svarm001@umaryland.edu","institution_ids":["https://openalex.org/I126744593"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004230977","display_name":"\u0421. \u0418. \u041a\u043e\u0432\u0442\u0443\u043d","orcid":"https://orcid.org/0000-0002-5492-882X","observed_orcids":["https://orcid.org/0000-0002-5492-882X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Svitlana Kovtun","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.0077,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.73555545,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"19","issue":"15","first_page":"493","last_page":"500"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.6934000253677368,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.6934000253677368,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11480","display_name":"Connexins and lens biology","score":0.2012999951839447,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12533","display_name":"Aldose Reductase and Taurine","score":0.01269999984651804,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/caffeine","display_name":"caffeine","score":1.0},{"id":"https://openalex.org/keywords/microrna","display_name":"microRNA","score":0.5220000147819519},{"id":"https://openalex.org/keywords/cataract","display_name":"cataract","score":0.7229999899864197},{"id":"https://openalex.org/keywords/lens","display_name":"lens","score":0.878000020980835},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.9490000009536743},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8069999814033508},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.8859999775886536},{"id":"https://openalex.org/keywords/galactosemia","display_name":"galactosemia","score":0.7440000176429749},{"id":"https://openalex.org/keywords/protective-effect","display_name":"protective effect","score":0.414000004529953}],"concepts":[{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.7722502946853638},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.7117683291435242},{"id":"https://openalex.org/C2778500429","wikidata":"https://www.wikidata.org/wiki/Q60235","display_name":"Caffeine","level":2,"score":0.6296916007995605},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6020894646644592},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5403260588645935},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5352652072906494},{"id":"https://openalex.org/C2776151105","wikidata":"https://www.wikidata.org/wiki/Q898814","display_name":"Oxidative stress","level":2,"score":0.44289225339889526},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.441912442445755},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40799158811569214},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34809979796409607},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3287731409072876},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.30069226026535034},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.17035943269729614}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002110","descriptor_name":"Caffeine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002386","descriptor_name":"Cataract","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002386","descriptor_name":"Cataract","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002386","descriptor_name":"Cataract","qualifier_ui":"Q000517","qualifier_name":"prevention & control","is_major_topic":false},{"descriptor_ui":"D004195","descriptor_name":"Disease Models, Animal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005690","descriptor_name":"Galactose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005693","descriptor_name":"Galactosemias","qualifier_ui":"Q000150","qualifier_name":"complications","is_major_topic":true},{"descriptor_ui":"D005693","descriptor_name":"Galactosemias","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005693","descriptor_name":"Galactosemias","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007908","descriptor_name":"Lens, Crystalline","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D007908","descriptor_name":"Lens, Crystalline","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008815","descriptor_name":"Mice, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D018384","descriptor_name":"Oxidative Stress","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"pmh:oai:europepmc.org:2578863","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC3580991","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:23441122","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23441122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3580991","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3580991/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},{"id":"mag:176884152","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23441122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null},{"id":"mag:756997496","doi":null,"is_oa":false,"landing_page_url":"https://iovs.arvojournals.org/article.aspx?articleid=2147707","pdf_url":null,"source":{"id":"https://openalex.org/S76304953","display_name":"Investigative Ophthalmology & Visual Science","issn_l":"0146-0404","issn":["0146-0404","1552-5783"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310321950","host_organization_name":"Cadmus Press","host_organization_lineage":["https://openalex.org/P4310321950"],"host_organization_lineage_names":["Cadmus Press"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Investigative Ophthalmology & Visual Science","raw_type":null}],"best_oa_location":{"id":"pmh:oai:europepmc.org:2578863","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC3580991","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[{"score":0.8999999761581421,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":103,"referenced_works":["https://openalex.org/W76961235","https://openalex.org/W92638324","https://openalex.org/W1498591505","https://openalex.org/W1498683119","https://openalex.org/W1515108121","https://openalex.org/W1517504372","https://openalex.org/W1523013418","https://openalex.org/W1805975457","https://openalex.org/W1874460601","https://openalex.org/W1910188440","https://openalex.org/W1964520813","https://openalex.org/W1968664427","https://openalex.org/W1971389338","https://openalex.org/W1978337436","https://openalex.org/W1980543376","https://openalex.org/W1983680438","https://openalex.org/W1987330691","https://openalex.org/W1992968751","https://openalex.org/W1995006312","https://openalex.org/W1998163977","https://openalex.org/W2000444665","https://openalex.org/W2003888115","https://openalex.org/W2005767645","https://openalex.org/W2009631046","https://openalex.org/W2011684607","https://openalex.org/W2012862746","https://openalex.org/W2013117701","https://openalex.org/W2015423537","https://openalex.org/W2015497016","https://openalex.org/W2019023722","https://openalex.org/W2020267609","https://openalex.org/W2024586219","https://openalex.org/W2025699701","https://openalex.org/W2030920510","https://openalex.org/W2031816239","https://openalex.org/W2033327522","https://openalex.org/W2034293061","https://openalex.org/W2037141750","https://openalex.org/W2039115551","https://openalex.org/W2051403674","https://openalex.org/W2052875009","https://openalex.org/W2059240823","https://openalex.org/W2060640359","https://openalex.org/W2062925001","https://openalex.org/W2066001559","https://openalex.org/W2068553992","https://openalex.org/W2071164257","https://openalex.org/W2072160272","https://openalex.org/W2074012960","https://openalex.org/W2075610353","https://openalex.org/W2077408537","https://openalex.org/W2078651468","https://openalex.org/W2083381199","https://openalex.org/W2084085461","https://openalex.org/W2084180808","https://openalex.org/W2084972185","https://openalex.org/W2086834797","https://openalex.org/W2087124521","https://openalex.org/W2088824478","https://openalex.org/W2090070787","https://openalex.org/W2092601519","https://openalex.org/W2092713330","https://openalex.org/W2093279030","https://openalex.org/W2099130114","https://openalex.org/W2099525065","https://openalex.org/W2101059550","https://openalex.org/W2104014840","https://openalex.org/W2106891590","https://openalex.org/W2107033535","https://openalex.org/W2108072925","https://openalex.org/W2108931848","https://openalex.org/W2114170469","https://openalex.org/W2115050468","https://openalex.org/W2128115589","https://openalex.org/W2128822428","https://openalex.org/W2130979840","https://openalex.org/W2131726212","https://openalex.org/W2134274931","https://openalex.org/W2137360803","https://openalex.org/W2138664009","https://openalex.org/W2139346977","https://openalex.org/W2140423650","https://openalex.org/W2143959688","https://openalex.org/W2145356433","https://openalex.org/W2147924866","https://openalex.org/W2148039314","https://openalex.org/W2158586551","https://openalex.org/W2160907352","https://openalex.org/W2162674813","https://openalex.org/W2165764599","https://openalex.org/W2168377537","https://openalex.org/W2169659025","https://openalex.org/W2172189733","https://openalex.org/W2201344815","https://openalex.org/W2221759143","https://openalex.org/W2273533551","https://openalex.org/W2275393226","https://openalex.org/W2339175250","https://openalex.org/W2363073682","https://openalex.org/W4237272779","https://openalex.org/W4248932937","https://openalex.org/W4285719527","https://openalex.org/W4300124633"],"related_works":["https://openalex.org/W1498591505","https://openalex.org/W2142289389","https://openalex.org/W7099764796","https://openalex.org/W7095716516","https://openalex.org/W4403026251","https://openalex.org/W2264775881","https://openalex.org/W2170215115","https://openalex.org/W1458220671","https://openalex.org/W7120650405","https://openalex.org/W2583081782"],"abstract_inverted_index":{"PURPOSE:":[0],"Previous":[1],"studies":[2],"have":[3],"shown":[4],"that":[5],"caffeine":[6,200,208],"prevents":[7],"the":[8,36,39,79,84,127,137,142,146,163,181,204],"formation":[9],"of":[10,23,38,41,96,148,188,207,218],"cataracts":[11],"induced":[12],"by":[13,51,98,174],"a":[14,61,66,112],"high-galactose":[15],"diet":[16,64,67],"and":[17,48,83,87,93,193,198,221],"consequent":[18,222],"oxidative":[19],"stress.":[20],"The":[21,166],"objective":[22],"this":[24,30],"study":[25],"was":[26,172],"to":[27,45,136,160,212,215],"investigate":[28],"if":[29],"protective":[31],"effect":[32,206],"is":[33],"reflected":[34],"in":[35,111,141,156,162],"attenuation":[37],"transcription":[40],"microRNAs":[42],"(miRNAs)":[43],"known":[44],"induce":[46],"apoptosis":[47],"cell":[49],"death":[50],"gene":[52,223],"silencing.":[53,224],"METHODS:":[54],"Young":[55],"CD-1":[56],"mice":[57],"were":[58,81,106,129,153],"fed":[59],"either":[60],"normal":[62,164],"laboratory":[63],"or":[65,72],"containing":[68],"25%":[69],"galactose":[70],"with":[71,109],"without":[73],"1%":[74],"caffeine.":[75,175],"One":[76],"week":[77],"later,":[78],"animals":[80],"euthanized,":[82],"lenses":[85,128,158],"isolated":[86],"promptly":[88],"processed":[89],"for":[90,131,180],"RNA":[91],"isolation":[92],"subsequent":[94],"preparation":[95],"cDNAs":[97,105],"reverse":[99],"transcriptase":[100],"reaction.":[101],"Mature":[102],"miRNA":[103,138],"(miR)-specific":[104],"then":[107],"quantified":[108],"PCR":[110,119],"96-well":[113],"microRNA-specific":[114],"cassette":[115,143],"using":[116],"an":[117],"ABI7900HT":[118],"machine.":[120],"RESULTS:":[121],"As":[122],"expected":[123],"from":[124],"previous":[125],"studies,":[126],"positive":[130],"all":[132],"84":[133],"miRs":[134,152,220],"corresponding":[135],"probes":[139],"present":[140],"wells.":[144],"However,":[145],"levels":[147],"at":[149],"least":[150],"19":[151],"significantly":[154],"elevated":[155],"galactosemic":[157],"compared":[159],"those":[161],"lenses.":[165],"majority":[167],"are":[168],"proapoptotic.":[169],"Such":[170],"elevation":[171,187,217],"inhibited":[173],"This":[176],"has":[177],"been":[178],"demonstrated":[179],"first":[182],"time.":[183],"CONCLUSIONS:":[184],"Since":[185],"aberrant":[186],"miRNAs":[189],"silences":[190],"various":[191],"genes":[192],"consequently":[194],"deactivates":[195],"protein":[196],"translation,":[197],"since":[199],"downregulates":[201],"such":[202],"aberration,":[203],"beneficial":[205],"could":[209],"be":[210],"attributed":[211],"its":[213],"ability":[214],"suppress":[216],"toxic":[219]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}