{"id":"https://openalex.org/W1760637495","doi":"https://doi.org/10.1152/ajpgi.1994.266.4.g647","title":"Brush-border tyrosine phosphorylation stimulates ileal neutral NaCl absorption and brush-border Na(+)-H+ exchange","display_name":"Brush-border tyrosine phosphorylation stimulates ileal neutral NaCl absorption and brush-border Na(+)-H+ exchange","publication_year":1994,"publication_date":"1994-04-01","ids":{"openalex":"https://openalex.org/W1760637495","doi":"https://doi.org/10.1152/ajpgi.1994.266.4.g647","mag":"1760637495","pmid":"https://pubmed.ncbi.nlm.nih.gov/8179002"},"language":"en","primary_location":{"id":"doi:10.1152/ajpgi.1994.266.4.g647","doi":"https://doi.org/10.1152/ajpgi.1994.266.4.g647","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpgi.1994.266.4.g647","pdf_url":null,"source":{"id":"https://openalex.org/S34136372","display_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","issn_l":"0193-1857","issn":["0193-1857","1522-1547"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320155","host_organization_name":"American Physiological Society","host_organization_lineage":["https://openalex.org/P4310320155"],"host_organization_lineage_names":["American Physiological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044859707","display_name":"Mark Donowitz","orcid":"https://orcid.org/0000-0003-0477-8824","observed_orcids":["https://orcid.org/0000-0003-0477-8824"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Donowitz","raw_affiliation_strings":["Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113605824","display_name":"J. L. Montgomery","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. L. Montgomery","raw_affiliation_strings":["Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113508015","display_name":"M. S. Walker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. S. Walker","raw_affiliation_strings":["Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"last","author":{"id":null,"display_name":"M. E. Cohen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. E. Cohen","raw_affiliation_strings":["Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Johns Hopkins University School of Medicine,Baltimore, Maryland 21205","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4246,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.50720216,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"266","issue":"4","first_page":"G647","last_page":"G656"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tyrosine-phosphorylation","display_name":"tyrosine phosphorylation","score":0.8740000128746033},{"id":"https://openalex.org/keywords/tyrosine-kinase","display_name":"tyrosine kinase","score":0.8240000009536743},{"id":"https://openalex.org/keywords/genistein","display_name":"genistein","score":0.8849999904632568},{"id":"https://openalex.org/keywords/genistin","display_name":"genistin","score":0.8019999861717224},{"id":"https://openalex.org/keywords/epidermal-growth-factor","display_name":"epidermal growth factor","score":0.9639999866485596},{"id":"https://openalex.org/keywords/a23187","display_name":"A23187","score":0.9210000038146973},{"id":"https://openalex.org/keywords/camp","display_name":"cyclic AMP","score":0.8989999890327454},{"id":"https://openalex.org/keywords/intestinal-epithelial-cells","display_name":"intestinal epithelial cells","score":0.7360000014305115},{"id":"https://openalex.org/keywords/chloride-secretion","display_name":"chloride secretion","score":0.6669999957084656},{"id":"https://openalex.org/keywords/short-circuit-current","display_name":"short-circuit current","score":0.7440000176429749},{"id":"https://openalex.org/keywords/signal-transduction","display_name":"signal transduction","score":0.7279999852180481},{"id":"https://openalex.org/keywords/brush-border-membrane-vesicles","display_name":"brush border membrane vesicles","score":0.8240000009536743},{"id":"https://openalex.org/keywords/rabbit-ileum","display_name":"rabbit ileum","score":0.7649999856948853}],"concepts":[{"id":"https://openalex.org/C2778298627","wikidata":"https://www.wikidata.org/wiki/Q415957","display_name":"Genistein","level":2,"score":0.8974955081939697},{"id":"https://openalex.org/C5426402","wikidata":"https://www.wikidata.org/wiki/Q2462753","display_name":"Brush border","level":4,"score":0.7526541948318481},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5705035924911499},{"id":"https://openalex.org/C2778820342","wikidata":"https://www.wikidata.org/wiki/Q906415","display_name":"Tyrosine-kinase inhibitor","level":3,"score":0.49795961380004883},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.46925613284111023},{"id":"https://openalex.org/C42362537","wikidata":"https://www.wikidata.org/wiki/Q87099740","display_name":"Tyrosine kinase","level":3,"score":0.45448777079582214},{"id":"https://openalex.org/C2777504804","wikidata":"https://www.wikidata.org/wiki/Q5533221","display_name":"Genistin","level":4,"score":0.4468280076980591},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.38958466053009033},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.37713488936424255},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.36829674243927},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3583265244960785},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3075527548789978},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.15877020359039307},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.09027692675590515},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.0},{"id":"https://openalex.org/C2780955279","wikidata":"https://www.wikidata.org/wiki/Q408732","display_name":"Daidzein","level":3,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000001","descriptor_name":"Calcimycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001692","descriptor_name":"Biological Transport","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007082","descriptor_name":"Ileum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007408","descriptor_name":"Intestinal Absorption","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D007529","descriptor_name":"Isoflavones","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008871","descriptor_name":"Microvilli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011505","descriptor_name":"Protein-Tyrosine Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012704","descriptor_name":"Serous Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":"Q000493","qualifier_name":"pharmacokinetics","is_major_topic":true},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015124","descriptor_name":"8-Bromo Cyclic Adenosine Monophosphate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017923","descriptor_name":"Sodium-Hydrogen Exchangers","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D017923","descriptor_name":"Sodium-Hydrogen Exchangers","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019833","descriptor_name":"Genistein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1152/ajpgi.1994.266.4.g647","doi":"https://doi.org/10.1152/ajpgi.1994.266.4.g647","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpgi.1994.266.4.g647","pdf_url":null,"source":{"id":"https://openalex.org/S34136372","display_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","issn_l":"0193-1857","issn":["0193-1857","1522-1547"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320155","host_organization_name":"American Physiological Society","host_organization_lineage":["https://openalex.org/P4310320155"],"host_organization_lineage_names":["American Physiological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","raw_type":"journal-article"},{"id":"pmid:8179002","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8179002","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7142867022","display_name":"Diarrheal Disease: Physiologic Approach to Treatment","funder_award_id":"R01DK026523","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2031928941","https://openalex.org/W2091301887","https://openalex.org/W2036632248","https://openalex.org/W2416432777","https://openalex.org/W2219409747","https://openalex.org/W296347082","https://openalex.org/W2274018045","https://openalex.org/W2058839243","https://openalex.org/W2134766531","https://openalex.org/W2080547929"],"abstract_inverted_index":{"The":[0],"drug":[1,101],"genistein,":[2],"a":[3,12,40,100,191,225,295],"tyrosine":[4],"(Tyr)":[5],"kinase":[6,110,193,232,298],"inhibitor,":[7],"was":[8],"used":[9],"to":[10,63,76,105,242],"define":[11],"role":[13],"for":[14,173],"Tyr":[15,109,192,227,231,261,297],"phosphorylation":[16],"in":[17,26,170,196,235,251,270,282,301,305],"regulation":[18,132],"of":[19,43,74,133,140,145,238,255,272,285,294],"basal":[20],"and":[21,52,144,179,203,276,280],"stimulated":[22,121],"neutral":[23,69,134,200,244,273],"NaCl":[24,70,122,135,201,210,245,274,286],"absorption":[25,202,211,246,275],"rabbit":[27],"ileum.":[28],"Brush-border":[29],"vesicles":[30,38,78],"contain":[31],"Tyr-phosphorylated":[32],"peptides.":[33],"Genistein":[34],"freeze-thawed":[35],"into":[36],"the":[37,56,64,138,151,162,168,217,221,236,252,291],"caused":[39,154],"concentration-dependent":[41],"inhibition":[42,284],"at":[44],"least":[45],"three":[46,175],"peptides":[47],"with":[48,55,102],"M(r)":[49],"111,000,":[50],"83,000,":[51],"80,000.":[53],"Studied":[54],"Ussing":[57],"chamber-voltage":[58],"clamp":[59],"technique,":[60],"genistein":[61,75,106,125],"added":[62],"ileal":[65,81,199],"mucosal":[66,116,184],"surface":[67],"inhibited":[68,84,182],"absorption.":[71,123,287],"Direct":[72],"addition":[73],"brush-border":[77,85,204,226,230,277,296],"made":[79],"from":[80,216],"villus":[82],"cells":[83],"Na(+)-H+":[86,205,278],"exchange":[87,279],"but":[88,107,114,126,148,220,247],"not":[89,96,115,127,149,249],"D-glucose-stimulated":[90],"Na+":[91],"uptake.":[92],"These":[93,186,288],"effects":[94,165,254],"were":[95,181],"duplicated":[97],"by":[98,155,167,183,212],"genistin,":[99],"similar":[103],"structure":[104],"lacking":[108],"inhibiting":[111,137],"properties.":[112],"Serosal":[113],"epidermal":[117],"growth":[118],"factor":[119],"(EGF)":[120],"Mucosal":[124],"genistin":[128],"also":[129,223,281],"altered":[130],"second-messenger":[131],"absorption,":[136],"effect":[139,214,222],"Ca2+":[141,239],"ionophore":[142,240],"A-23187":[143,241],"serosal":[146,218],"EGF":[147,208],"affecting":[150],"transport":[152,253],"changes":[153],"8-bromoadenosine":[156],"3',5'-cyclic":[157],"monophosphate":[158],"(8-BrcAMP).":[159],"In":[160],"contrast,":[161],"Cl":[163],"secretory":[164],"indicated":[166],"increase":[169],"short-circuit":[171],"current":[172],"all":[174],"agents,":[176],"A-23187,":[177],"EGF,":[178],"8-BrcAMP,":[180],"genistein.":[185],"results":[187],"suggest":[188],"that":[189,260],"1)":[190],"is":[194,233,248,268],"involved":[195,234,250,269,300],"basally":[197],"stimulating":[198],"exchange;":[206],"2)":[207],"stimulates":[209],"an":[213],"exerted":[215],"surface,":[219],"involves":[224],"kinase;":[228],"3)":[229],"ability":[237],"inhibit":[243],"cAMP.":[256],"This":[257],"study":[258],"suggests":[259],"kinase(s)":[262],"acting":[263],"over":[264],"short":[265],"time":[266],"periods":[267],"stimulation":[271],"Ca(2+)-induced":[283],"studies":[289],"represent":[290],"first":[292],"example":[293],"being":[299],"short-term":[302],"signal":[303],"transduction":[304],"intestinal":[306],"epithelial":[307],"cells.":[308]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}