{"id":"https://openalex.org/W17065246","doi":null,"title":"Catalytic properties of tryptophanless recombinant horseradish peroxidase.","display_name":"Catalytic properties of tryptophanless recombinant horseradish peroxidase.","publication_year":2000,"publication_date":"2000-05-01","ids":{"openalex":"https://openalex.org/W17065246","mag":"17065246","pmid":"https://pubmed.ncbi.nlm.nih.gov/10851036"},"language":"en","primary_location":{"id":"pmid:10851036","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10851036","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry. Biokhimiia","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5020827862","display_name":"\u041e. \u0412. \u0418\u0433\u043d\u0430\u0442\u0435\u043d\u043a\u043e","orcid":"https://orcid.org/0000-0002-6353-2552","observed_orcids":["https://orcid.org/0000-0002-6353-2552"]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"O V Ignatenko","raw_affiliation_strings":["Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042379176","display_name":"Irina G. Gazaryan","orcid":"https://orcid.org/0000-0002-9670-5579","observed_orcids":["https://orcid.org/0000-0002-9670-5579"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I G Gazaryan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035287899","display_name":"E. A. Mareeva","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E A Mareeva","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113239881","display_name":"Tatiana A. Chubar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T A Chubar","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007907628","display_name":"V. A. Fechina","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V A Fechina","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081160340","display_name":"Pavel A. Savitsky","orcid":"https://orcid.org/0000-0001-8584-7750","observed_orcids":["https://orcid.org/0000-0001-8584-7750"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P A Savitsky","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110014986","display_name":"A. Rojkova","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A M Rojkova","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5035777057","display_name":"V. I. Tishkov","orcid":"https://orcid.org/0000-0002-6997-7229","observed_orcids":["https://orcid.org/0000-0002-6997-7229"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V I Tishkov","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.00163236,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"65","issue":"5","first_page":"583","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.7695000171661377,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.7695000171661377,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11136","display_name":"Enzyme-mediated dye degradation","score":0.05620000138878822,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.05260000005364418,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/horseradish-peroxidase","display_name":"horseradish peroxidase","score":0.9769999980926514},{"id":"https://openalex.org/keywords/tryptophan-residues","display_name":"tryptophan residues","score":0.7720000147819519},{"id":"https://openalex.org/keywords/recombinant-enzyme","display_name":"recombinant enzyme","score":0.6790000200271606},{"id":"https://openalex.org/keywords/abts-oxidation","display_name":"ABTS oxidation","score":0.6629999876022339},{"id":"https://openalex.org/keywords/guaiacol-oxidation","display_name":"guaiacol oxidation","score":0.9980000257492065},{"id":"https://openalex.org/keywords/o-phenylenediamine-oxidation","display_name":"o-phenylenediamine oxidation","score":0.996999979019165},{"id":"https://openalex.org/keywords/reaction-kinetics","display_name":"reaction kinetics","score":0.6269999742507935},{"id":"https://openalex.org/keywords/enzyme-substrate-complex","display_name":"enzyme-substrate complex","score":0.7799999713897705},{"id":"https://openalex.org/keywords/electron-transfer","display_name":"electron transfer","score":0.7749999761581421},{"id":"https://openalex.org/keywords/product-dissociation","display_name":"product dissociation","score":0.6330000162124634},{"id":"https://openalex.org/keywords/heme-peroxidase","display_name":"heme peroxidase","score":0.6990000009536743}],"concepts":[{"id":"https://openalex.org/C2780199054","wikidata":"https://www.wikidata.org/wiki/Q412403","display_name":"Guaiacol","level":3,"score":0.9473038911819458},{"id":"https://openalex.org/C2777676563","wikidata":"https://www.wikidata.org/wiki/Q413665","display_name":"Horseradish peroxidase","level":3,"score":0.8912081718444824},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8359716534614563},{"id":"https://openalex.org/C2781031786","wikidata":"https://www.wikidata.org/wiki/Q287582","display_name":"ABTS","level":4,"score":0.7991839647293091},{"id":"https://openalex.org/C162008176","wikidata":"https://www.wikidata.org/wiki/Q419305","display_name":"Peroxidase","level":3,"score":0.6649919152259827},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.49185872077941895},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.49003875255584717},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.474881112575531},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.46548476815223694},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.37473931908607483},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.34452205896377563},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.32492536306381226},{"id":"https://openalex.org/C2778004101","wikidata":"https://www.wikidata.org/wiki/Q133948","display_name":"Antioxidant","level":2,"score":0.08431357145309448},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.07443439960479736},{"id":"https://openalex.org/C2781401929","wikidata":"https://www.wikidata.org/wiki/Q5205953","display_name":"DPPH","level":3,"score":0.05387762188911438}],"mesh":[{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:10851036","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10851036","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry. Biokhimiia","raw_type":"Journal Article"},{"id":"mag:17065246","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10851036","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W1965493010"],"related_works":["https://openalex.org/W2413346898","https://openalex.org/W2085347147","https://openalex.org/W2010897529","https://openalex.org/W2086371387","https://openalex.org/W1965493010","https://openalex.org/W1970431680","https://openalex.org/W2134256910","https://openalex.org/W4205412867","https://openalex.org/W2036227691","https://openalex.org/W3005550540"],"abstract_inverted_index":{"Heme-containing":[0],"plant":[1],"peroxidases":[2],"(EC":[3],"1.11.1.7)":[4],"contain":[5],"a":[6],"highly":[7],"conserved":[8],"single":[9,107],"tryptophan":[10],"residue.":[11],"Its":[12],"replacement":[13],"with":[14,80],"Phe":[15],"in":[16,28,43,61,110,119],"recombinant":[17,40],"horseradish":[18,41],"peroxidase":[19,42],"(rHRP)":[20],"increased":[21],"the":[22,25,44,62,68,81,87,90,94,103,106],"stability":[23],"of":[24,34,46,105,112,124],"mutant":[26,39],"enzyme":[27,82],"acid":[29],"media.":[30],"The":[31,99],"kinetic":[32,120],"properties":[33],"native,":[35],"wild-type,":[36],"and":[37,52,114,116,127],"W117F":[38],"reactions":[45],"ammonium":[47],"2,":[48],"2;-azino-bis(3-ethylbenzthiazoline-6-sulfonate)":[49],"(ABTS),":[50],"guaiacol,":[51,126],"o-phenylenediamine":[53],"oxidation":[54,111,123],"are":[55],"very":[56],"similar.":[57],"However,":[58],"significant":[59],"changes":[60],"reaction":[63],"rate":[64],"constant":[65],"characteristic":[66],"for":[67,122],"monomolecular":[69],"rate-limiting":[70],"step":[71],"ascribed":[72],"either":[73],"to":[74,89],"product":[75],"dissociation":[76],"from":[77,86],"its":[78],"complex":[79,96],"or":[83],"electron":[84],"transfer":[85],"substrate":[88],"active":[91],"site":[92],"within":[93],"Michaelis":[95],"were":[97],"observed.":[98],"data":[100],"indirectly":[101],"indicate":[102],"participation":[104],"Trp":[108],"residue":[109],"ABTS":[113],"guaiacol":[115],"possible":[117],"differences":[118],"mechanisms":[121],"ABTS,":[125],"o-phenylenediamine.":[128]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
