{"id":"https://openalex.org/W1674052188","doi":null,"title":"Exogenous expression of human SGLT1 exhibits aggregations in sodium dodecyl sulfate polyacrylamide gel electrophoresis.","display_name":"Exogenous expression of human SGLT1 exhibits aggregations in sodium dodecyl sulfate polyacrylamide gel electrophoresis.","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W1674052188","mag":"1674052188","pmid":"https://pubmed.ncbi.nlm.nih.gov/23724167","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3665917"},"language":"en","primary_location":{"id":"pmh:oai:europepmc.org:2662244","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3665917","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3665917","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058076484","display_name":"Wei\u2010Chien Huang","orcid":"https://orcid.org/0000-0001-6467-8716","observed_orcids":["https://orcid.org/0000-0001-6467-8716"]},"institutions":[{"id":"https://openalex.org/I143578492","display_name":"Asia University","ror":"https://ror.org/038a1tp19","country_code":"TW","type":"education","lineage":["https://openalex.org/I143578492"]},{"id":"https://openalex.org/I184693016","display_name":"China Medical University","ror":"https://ror.org/00v408z34","country_code":"TW","type":"education","lineage":["https://openalex.org/I184693016"]},{"id":"https://openalex.org/I4210126829","display_name":"China Medical University Hospital","ror":"https://ror.org/0368s4g32","country_code":"TW","type":"healthcare","lineage":["https://openalex.org/I4210126829"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Wei-Chien Huang","raw_affiliation_strings":["Center for Molecular Medicine, China Medical University Hospital Taichung 404, Taiwan ; Graduate Institute of Cancer Biology, China Medical University Taichung 404, Taiwan ; the Ph.D. program for Cancer Biology and Drug Discovery, China Medical University Taichung 404, Taiwan ; Department of Biotechnology, Asia University Taichung 413, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Molecular Medicine, China Medical University Hospital Taichung 404, Taiwan ; Graduate Institute of Cancer Biology, China Medical University Taichung 404, Taiwan ; the Ph.D. program for Cancer Biology and Drug Discovery, China Medical University Taichung 404, Taiwan ; Department of Biotechnology, Asia University Taichung 413, Taiwan","institution_ids":["https://openalex.org/I143578492","https://openalex.org/I184693016","https://openalex.org/I4210126829"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110050369","display_name":"Sheng-Chieh Hsu","orcid":"https://orcid.org/0000-0003-2637-1136","observed_orcids":["https://orcid.org/0000-0003-2637-1136"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sheng-Chie Hsu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047317233","display_name":"Shyh-Jer Huang","orcid":"https://orcid.org/0000-0002-6457-2003","observed_orcids":["https://orcid.org/0000-0002-6457-2003"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shyh-Jer Huang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057593261","display_name":"Yun\u2010Ju Chen","orcid":"https://orcid.org/0000-0002-0659-2110","observed_orcids":["https://orcid.org/0000-0002-0659-2110"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yun-Ju Chen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109475493","display_name":"Yu-Chun Hsiao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yu-Chun Hsiao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100370310","display_name":"Weihua Zhang","orcid":"https://orcid.org/0000-0002-4165-5260","observed_orcids":["https://orcid.org/0000-0002-4165-5260"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Weihua Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110018505","display_name":"Isaiah J. Fidler","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Isaiah J Fidler","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5076834432","display_name":"Mien\u2010Chie Hung","orcid":"https://orcid.org/0000-0003-4317-4740","observed_orcids":["https://orcid.org/0000-0003-4317-4740"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mien-Chie Hung","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3299,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.5277283,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"5","issue":"4","first_page":"441","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.37279999256134033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.37279999256134033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.3425000011920929,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.032999999821186066,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sglt1","display_name":"SGLT1","score":0.9919999837875366},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.7950000166893005},{"id":"https://openalex.org/keywords/western-blot-detection","display_name":"Western blot detection","score":0.5830000042915344},{"id":"https://openalex.org/keywords/glucose-uptake","display_name":"glucose uptake","score":0.8199999928474426},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.6380000114440918},{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.7620000243186951},{"id":"https://openalex.org/keywords/fusion-tags","display_name":"fusion tags","score":0.3970000147819519}],"concepts":[{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.7329240441322327},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.5283104777336121},{"id":"https://openalex.org/C161573976","wikidata":"https://www.wikidata.org/wiki/Q420277","display_name":"Glucose transporter","level":3,"score":0.5035964846611023},{"id":"https://openalex.org/C2777866211","wikidata":"https://www.wikidata.org/wiki/Q5572349","display_name":"Glucose uptake","level":3,"score":0.4724600315093994},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.4582192003726959},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.4547497630119324},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4447932243347168},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.4426155686378479},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.42582905292510986},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.4225842356681824},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4200100898742676},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32471245527267456},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2435280680656433},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1690237820148468},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.10123240947723389},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.1006685197353363},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:europepmc.org:2662244","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3665917","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:23724167","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23724167","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American journal of translational research","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3665917","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3665917/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Transl Res","raw_type":"Text"},{"id":"mag:1674052188","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23724167","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:europepmc.org:2662244","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3665917","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7043661956","display_name":"Growth Factor Receptor in Breast Cancer Progression and Metabolic Regulation","funder_award_id":"P01CA099031","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W48239396","https://openalex.org/W1484764833","https://openalex.org/W1522571942","https://openalex.org/W1901783123","https://openalex.org/W1978697833","https://openalex.org/W1980832022","https://openalex.org/W1983298232","https://openalex.org/W1987412156","https://openalex.org/W1988293842","https://openalex.org/W1996507796","https://openalex.org/W1999159723","https://openalex.org/W2006700297","https://openalex.org/W2007169088","https://openalex.org/W2009307442","https://openalex.org/W2014560547","https://openalex.org/W2016066705","https://openalex.org/W2022737510","https://openalex.org/W2028976116","https://openalex.org/W2043680485","https://openalex.org/W2046775283","https://openalex.org/W2053439872","https://openalex.org/W2059247506","https://openalex.org/W2063986131","https://openalex.org/W2089016384","https://openalex.org/W2091674644","https://openalex.org/W2094882473","https://openalex.org/W2096246094","https://openalex.org/W2100447481","https://openalex.org/W2105526739","https://openalex.org/W2128788229","https://openalex.org/W2143890940","https://openalex.org/W2144538410","https://openalex.org/W2149542756","https://openalex.org/W2426421382","https://openalex.org/W2468861408","https://openalex.org/W4243903519"],"related_works":["https://openalex.org/W2122064456","https://openalex.org/W1042127682","https://openalex.org/W2745799264","https://openalex.org/W1635144248","https://openalex.org/W1502257229","https://openalex.org/W2129621420","https://openalex.org/W2888856323","https://openalex.org/W2067898969","https://openalex.org/W2026214578","https://openalex.org/W2753412903"],"abstract_inverted_index":{"Sodium/glucose":[0],"co-transporter":[1],"1":[2],"(SGLT1),":[3],"which":[4,81,92,140],"actively":[5],"and":[6,22,24,47,118,144,157],"energy-dependently":[7],"uptakes":[8],"glucose,":[9],"plays":[10],"critical":[11],"roles":[12],"in":[13,105,128],"the":[14,45,58,88,113,124,134,154,161,178,190,237],"development":[15],"of":[16,49,53,57,150,160,181,192,207,215],"various":[17],"diseases":[18],"including":[19],"diabetes":[20],"mellitus":[21],"cancer,":[23],"has":[25,40],"been":[26,41],"viewed":[27],"as":[28],"a":[29,83],"promising":[30],"therapeutic":[31],"target":[32],"for":[33,85],"these":[34],"diseases.":[35],"Protein-protein":[36],"interaction":[37],"with":[38,165],"EGFR":[39,151],"shown":[42],"to":[43,61,231],"regulate":[44],"expression":[46,52],"activity":[48,156,180],"SGLT1.":[50,162],"Exogenous":[51],"SGLT1":[54,68,93,103,136,183,193,216],"is":[55,69,94,109],"one":[56],"essential":[59],"approaches":[60],"characterize":[62],"its":[63,77,171,175,186,227,232],"functions;":[64],"however,":[65],"exogenously":[66],"expressed":[67],"not":[70,174,196,222],"firmly":[71],"detectable":[72,225],"by":[73,91,198,205,235],"Western":[74,129],"blot":[75,130],"at":[76,123,170,226],"calculated":[78,125],"molecular":[79,89,126],"weight,":[80],"creates":[82],"hurdle":[84],"further":[86],"understanding":[87],"events":[90],"regulated.":[95],"In":[96],"this":[97],"study,":[98],"we":[99],"demonstrated":[100],"that":[101,133],"exogenous":[102,182,213],"functions":[104],"glucose-uptake":[106,155,179],"normally":[107,219],"but":[108,173,201,220],"consistently":[110,224],"detected":[111],"near":[112],"interface":[114],"between":[115],"stacking":[116],"gel":[117,120],"running":[119],"rather":[121],"than":[122],"weight":[127,229],"analysis,":[131],"suggesting":[132],"overexpressed":[135],"forms":[137],"SDS-resistant":[138,238],"aggregates,":[139],"cannot":[141],"be":[142,223],"denatured":[143],"effectively":[145],"separated":[146],"on":[147],"SDS-PAGE.":[148],"Co-expression":[149],"enhances":[152],"both":[153],"protein":[158,187],"level":[159],"However,":[163],"fusion":[164],"Flag":[166],"or":[167],"HA":[168],"tag":[169],"carboxy-":[172],"amino-terminus":[176],"abolished":[177],"without":[184],"affecting":[185],"level.":[188],"Furthermore,":[189],"solubility":[191],"aggregates":[194],"was":[195,202],"affected":[197],"other":[199],"detergents":[200],"partially":[203],"improved":[204],"inhibition":[206],"o-link":[208],"glycosylation.":[209],"These":[210],"findings":[211],"suggested":[212],"overexpression":[214],"can":[217],"function":[218],"may":[221],"formula":[228],"due":[230],"gel-shift":[233],"behavior":[234],"forming":[236],"aggregates.":[239]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}