{"id":"https://openalex.org/W1669966701","doi":null,"title":"Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.","display_name":"Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.","publication_year":2008,"publication_date":"2008-04-01","ids":{"openalex":"https://openalex.org/W1669966701","mag":"1669966701","pmid":"https://pubmed.ncbi.nlm.nih.gov/19137093","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2361164"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2361164","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2361164","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2361164","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049259283","display_name":"John Gordon Bruno","orcid":"https://orcid.org/0000-0003-2745-2624","observed_orcids":["https://orcid.org/0000-0003-2745-2624"]},"institutions":[{"id":"https://openalex.org/I4210110010","display_name":"Operational Technologies Corporation (United States)","ror":"https://ror.org/01txpgc43","country_code":"US","type":"company","lineage":["https://openalex.org/I4210110010"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John G Bruno","raw_affiliation_strings":["Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX 78229, USA. john.bruno@otcorp.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX 78229, USA. john.bruno@otcorp.com","institution_ids":["https://openalex.org/I4210110010"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005240451","display_name":"Maria P. Carrillo","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Maria P Carrillo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5068575582","display_name":"Taylor Phillips","orcid":"https://orcid.org/0009-0004-3447-7584","observed_orcids":["https://orcid.org/0009-0004-3447-7584"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Taylor Phillips","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.896,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":{"value":0.68535863,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"19","issue":"2","first_page":"109","last_page":"15"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9833999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9833999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/foot-and-mouth-disease","display_name":"foot-and-mouth disease","score":0.800000011920929},{"id":"https://openalex.org/keywords/dna-aptamers","display_name":"DNA aptamers","score":0.9559999704360962},{"id":"https://openalex.org/keywords/rapid-virus-detection","display_name":"rapid virus detection","score":0.39899998903274536}],"concepts":[{"id":"https://openalex.org/C128972844","wikidata":"https://www.wikidata.org/wiki/Q418722","display_name":"Aptamer","level":2,"score":0.8937551975250244},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.8434196710586548},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.8042139410972595},{"id":"https://openalex.org/C2776759294","wikidata":"https://www.wikidata.org/wiki/Q2833068","display_name":"Alexa Fluor","level":3,"score":0.7738338708877563},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6362906694412231},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.577505350112915},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.48372724652290344},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4235283434391022},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.33266663551330566},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3021193742752075},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.2601715326309204},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.11921900510787964},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09189453721046448},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004196","descriptor_name":"Disease Outbreaks","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005536","descriptor_name":"Foot-and-Mouth Disease","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D005536","descriptor_name":"Foot-and-Mouth Disease","qualifier_ui":"Q000453","qualifier_name":"epidemiology","is_major_topic":false},{"descriptor_ui":"D005536","descriptor_name":"Foot-and-Mouth Disease","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029544","descriptor_name":"Foot-and-Mouth Disease Virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D029544","descriptor_name":"Foot-and-Mouth Disease Virus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D031541","descriptor_name":"Fluorescence Resonance Energy Transfer","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D036022","descriptor_name":"Capsid Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D052156","descriptor_name":"SELEX Aptamer Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:2361164","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2361164","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},{"id":"pmid:19137093","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19137093","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biomolecular techniques : JBT","raw_type":"Evaluation Study"},{"id":"mag:1669966701","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19137093","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2361164","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2361164","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W3145257564"],"related_works":["https://openalex.org/W4404828822","https://openalex.org/W7127299429","https://openalex.org/W7155065481","https://openalex.org/W7170231844","https://openalex.org/W4309565717","https://openalex.org/W2051371200","https://openalex.org/W2744266873","https://openalex.org/W4309740002","https://openalex.org/W4294344084","https://openalex.org/W4389224151"],"abstract_inverted_index":{"We":[0],"sought":[1],"to":[2,65,88],"develop":[3],"a":[4,76,89],"novel":[5],"competitive":[6,138],"fluorescence":[7],"resonance":[8],"energy":[9],"transfer":[10],"(FRET)-aptamer-based":[11],"strategy":[12],"for":[13,154],"detection":[14],"of":[15,35,72,91,94,146,157],"foot-and-mouth":[16],"(FMD)":[17],"disease":[18],"within":[19,82],"minutes.":[20],"A":[21,116],"14-amino-acid":[22],"peptide":[23],"from":[24,102],"the":[25,67,73,103,137,144,147,152,158],"VP1":[26],"structural":[27],"protein,":[28],"which":[29],"is":[30],"conserved":[31],"among":[32],"16":[33],"strains":[34],"O-serotype":[36],"FMD":[37,50,95],"virus,":[38],"was":[39,80,86],"synthesized":[40],"and":[41,63,85,118,134],"labeled":[42,54,124],"with":[43,55,125],"Black":[44],"Hole":[45],"Quencher-2":[46],"(BHQ-2)":[47],"dye.":[48],"Polyclonal":[49],"DNA":[51],"aptamers":[52,99],"were":[53,100,109,122],"Alexa":[56,126],"Fluor":[57,127],"546-14-dUTP":[58,128],"by":[59,129],"polymerase":[60,131],"chain":[61,132],"reaction":[62,133],"allowed":[64],"bind":[66],"BHQ-2-peptide":[68],"conjugate.":[69],"Following":[70],"purification":[71],"FRET-aptamer-peptide":[74],"complex,":[75],"\"lights":[77],"off\"":[78],"response":[79],"observed":[81],"10":[83],"minutes":[84],"sensitive":[87],"level":[90],"25-250":[92],"ng/mL":[93],"peptide.":[96],"Ten":[97],"candidate":[98,121],"sequenced":[101],"polyclonal":[104,148],"family.":[105],"The":[106],"aptamer":[107,120,159],"candidates":[108],"screened":[110],"in":[111,136],"an":[112],"enzyme-based":[113],"plate":[114],"assay.":[115],"high-":[117],"low-affinity":[119],"each":[123],"asymmetric":[130],"used":[135],"FRET":[139,149],"assay,":[140],"but":[141],"neither":[142],"matched":[143],"sensitivity":[145],"response,":[150],"indicating":[151],"need":[153],"further":[155],"screening":[156],"library.":[160]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}