{"id":"https://openalex.org/W166198506","doi":"https://doi.org/10.1023/a:1006876420980","title":"Flow cytometric characteristics of subchloroplast particles prepared by the action of various detergents","display_name":"Flow cytometric characteristics of subchloroplast particles prepared by the action of various detergents","publication_year":1997,"publication_date":"1997-09-01","ids":{"openalex":"https://openalex.org/W166198506","doi":"https://doi.org/10.1023/a:1006876420980","mag":"166198506"},"language":"en","primary_location":{"id":"doi:10.1023/a:1006876420980","doi":"https://doi.org/10.1023/a:1006876420980","is_oa":true,"landing_page_url":"https://doi.org/10.1023/a:1006876420980","pdf_url":"http://ps.ueb.cas.cz/doi/10.1023/A:1006876420980.pdf","source":{"id":"https://openalex.org/S201514771","display_name":"Photosynthetica","issn_l":"0300-3604","issn":["0300-3604","1573-9058"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","oarl","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310314217","host_organization_name":"Institute of Experimental Botany of the Czech Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310314217","https://openalex.org/P4310314171"],"host_organization_lineage_names":["Institute of Experimental Botany of the Czech Academy of Sciences","Czech Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Photosynthetica","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://ps.ueb.cas.cz/doi/10.1023/A:1006876420980.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088370916","display_name":"Na\u010fa Wilhelmov\u00e1","orcid":"https://orcid.org/0000-0002-5546-5513","observed_orcids":["https://orcid.org/0000-0002-5546-5513"]},"institutions":[{"id":"https://openalex.org/I202391551","display_name":"Czech Academy of Sciences","ror":"https://ror.org/053avzc18","country_code":"CZ","type":"government","lineage":["https://openalex.org/I202391551"]},{"id":"https://openalex.org/I4210156025","display_name":"Czech Academy of Sciences, Institute of Experimental Botany","ror":"https://ror.org/057br4398","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210156025"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"N. Wilhelmov\u00e1","raw_affiliation_strings":["Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha\u00a06, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha\u00a06, Czech Republic","institution_ids":["https://openalex.org/I202391551","https://openalex.org/I4210156025"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050504086","display_name":"S. Van\u011bk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"S. Van\u011bk","raw_affiliation_strings":["Department of Clinical Haematology of the Faculty Hospital of Charles University, Praha 2, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Haematology of the Faculty Hospital of Charles University, Praha 2, Czech Republic","institution_ids":["https://openalex.org/I21250087"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110117589","display_name":"Ji\u0159\u00ed Wilhelm","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"J. Wilhelm","raw_affiliation_strings":["2nd Medical School, Charles University, Praha 5, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2nd Medical School, Charles University, Praha 5, Czech Republic","institution_ids":["https://openalex.org/I21250087"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3140,"currency":"USD","value_usd":3140},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00879038,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"34","issue":"3","first_page":"447","last_page":"464"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.9315999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.9315999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.06310000270605087,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/subchloroplast-particles","display_name":"subchloroplast particles","score":0.8479999899864197},{"id":"https://openalex.org/keywords/common-bean","display_name":"Phaseolus vulgaris","score":0.8820000290870667},{"id":"https://openalex.org/keywords/vesicle-size-distribution","display_name":"vesicle size distribution","score":0.7770000100135803},{"id":"https://openalex.org/keywords/particle-density","display_name":"particle density","score":0.7549999952316284},{"id":"https://openalex.org/keywords/triton-x-100","display_name":"Triton X-100","score":0.8389999866485596},{"id":"https://openalex.org/keywords/digitonin","display_name":"digitonin","score":0.9160000085830688},{"id":"https://openalex.org/keywords/cetylpyridinium-chloride","display_name":"cetylpyridinium chloride","score":0.8759999871253967},{"id":"https://openalex.org/keywords/sodium-dodecyl-sulfate","display_name":"sodium dodecyl sulfate","score":0.8740000128746033},{"id":"https://openalex.org/keywords/percoll-gradient","display_name":"Percoll density gradient","score":0.6230000257492065}],"concepts":[{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.7643715143203735},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6437921524047852},{"id":"https://openalex.org/C101692577","wikidata":"https://www.wikidata.org/wiki/Q337889","display_name":"Thylakoid","level":4,"score":0.6289735436439514},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5819180607795715},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5322876572608948},{"id":"https://openalex.org/C2778920010","wikidata":"https://www.wikidata.org/wiki/Q410968","display_name":"Digitonin","level":3,"score":0.4882190525531769},{"id":"https://openalex.org/C187530423","wikidata":"https://www.wikidata.org/wiki/Q7140503","display_name":"Particle size","level":2,"score":0.4510103166103363},{"id":"https://openalex.org/C48679268","wikidata":"https://www.wikidata.org/wiki/Q3375169","display_name":"Percoll","level":3,"score":0.4382343292236328},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3830193281173706},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.349764347076416},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.24228736758232117},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22917836904525757},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14180243015289307},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.10162389278411865},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1023/a:1006876420980","doi":"https://doi.org/10.1023/a:1006876420980","is_oa":true,"landing_page_url":"https://doi.org/10.1023/a:1006876420980","pdf_url":"http://ps.ueb.cas.cz/doi/10.1023/A:1006876420980.pdf","source":{"id":"https://openalex.org/S201514771","display_name":"Photosynthetica","issn_l":"0300-3604","issn":["0300-3604","1573-9058"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","oarl","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310314217","host_organization_name":"Institute of Experimental Botany of the Czech Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310314217","https://openalex.org/P4310314171"],"host_organization_lineage_names":["Institute of Experimental Botany of the Czech Academy of Sciences","Czech Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Photosynthetica","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1023/a:1006876420980","doi":"https://doi.org/10.1023/a:1006876420980","is_oa":true,"landing_page_url":"https://doi.org/10.1023/a:1006876420980","pdf_url":"http://ps.ueb.cas.cz/doi/10.1023/A:1006876420980.pdf","source":{"id":"https://openalex.org/S201514771","display_name":"Photosynthetica","issn_l":"0300-3604","issn":["0300-3604","1573-9058"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","oarl","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310314217","host_organization_name":"Institute of Experimental Botany of the Czech Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310314217","https://openalex.org/P4310314171"],"host_organization_lineage_names":["Institute of Experimental Botany of the Czech Academy of Sciences","Czech Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Photosynthetica","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W166198506.pdf"},"referenced_works_count":7,"referenced_works":["https://openalex.org/W128312175","https://openalex.org/W652760414","https://openalex.org/W1974907033","https://openalex.org/W2016466877","https://openalex.org/W2019962788","https://openalex.org/W2075028972","https://openalex.org/W2145582285"],"related_works":["https://openalex.org/W652760414","https://openalex.org/W2153507437","https://openalex.org/W2242109477","https://openalex.org/W1988302426","https://openalex.org/W2090155040","https://openalex.org/W1992772016","https://openalex.org/W2021023243","https://openalex.org/W2262178477","https://openalex.org/W2077087019","https://openalex.org/W2187534576"],"abstract_inverted_index":{"Agranal":[0],"thylakoid":[1],"membranes":[2],"from":[3,158],"leaves":[4],"of":[5,70,72,101,121,132,139,141,173,175],"Phaseolus":[6],"vulgaris":[7],"L.":[8],"were":[9,42,96,147],"fragmented":[10],"using":[11],"seven":[12],"distinct":[13],"detergents:":[14],"digitonin,":[15],"Triton":[16],"X-100,":[17],"cetylpyridinium":[18],"chloride,":[19],"sodium":[20],"dodecylsulfate,":[21],"and":[22,26,51,84,145],"Zwittergents":[23],"3-12,":[24],"3-14,":[25],"3-16,":[27],"differing":[28],"in":[29,90,99,124],"chemical":[30],"composition":[31],"and/or":[32],"electric":[33],"charges.":[34],"Subchloroplast":[35],"particles":[36,95,140],"separated":[37],"on":[38,127],"a":[39,59,108,118],"Percoll":[40],"gradient":[41],"examined":[43],"by":[44,58,178],"flow":[45],"cytometry":[46],"to":[47],"determine":[48],"their":[49,85],"size":[50,54,75,86,110,144],"shape.":[52],"Vesicle":[53],"was":[55,87,156],"also":[56],"determined":[57],"haematological":[60],"analyzer,":[61],"which":[62],"produced":[63],"comparable":[64],"results.":[65],"Individual":[66],"green":[67],"bands":[68],"consisted":[69],"vesicles":[71,105],"fairly":[73],"wide":[74],"distribution.":[76],"Simple":[77],"direct":[78],"proportionality":[79],"between":[80],"the":[81,112,122,142,159,171],"particle":[82],"density":[83,146],"not":[88,148],"observed":[89],"any":[91],"case,":[92],"nevertheless,":[93],"bigger":[94],"more":[97],"abundant":[98],"fractions":[100],"higher":[102],"density.":[103],"Some":[104],"had":[106],"even":[107],"larger":[109],"than":[111],"original":[113],"thylakoids.":[114],"This":[115],"might":[116],"reflect":[117],"specific":[119],"action":[120],"detergents":[123],"low":[125],"concentrations":[126],"agranal":[128],"membranes,":[129],"with":[130],"incorporation":[131],"detergent":[133],"molecules":[134],"into":[135],"vesicles.":[136],"Inner":[137],"structures":[138],"same":[143],"necessarily":[149],"identical,":[150],"but":[151],"represented":[152],"several":[153],"populations,":[154],"as":[155],"apparent":[157],"side":[160],"scatter":[161],"analysis.":[162],"Flow":[163],"cytometric":[164],"analysis":[165],"can":[166],"thus":[167],"be":[168],"used":[169],"for":[170],"investigation":[172],"mechanisms":[174],"membrane":[176],"fragmentation":[177],"detergents.":[179]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}